| Definition | Burkholderia thailandensis E264 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_007651 |
| Length | 3,809,201 |
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The map label for this gene is gshA-1 [H]
Identifier: 83719941
GI number: 83719941
Start: 91752
End: 93398
Strand: Direct
Name: gshA-1 [H]
Synonym: BTH_I0087
Alternate gene names: 83719941
Gene position: 91752-93398 (Clockwise)
Preceding gene: 83721464
Following gene: 83718701
Centisome position: 2.41
GC content: 67.82
Gene sequence:
>1647_bases ATGCCGCGGATCGCCGAGCCCGCACTTGCATCAATGCCGAACAACATGACTTCCCGCCAGACAGACTTGTTGCAGCAACG CCTTGCCGTGTTGAGCACGAGCCCGACGCGCGAGCAGCTGCCTGACGGCCTGCGCGGCATCGAGAAGGAAAGCCTGCGCG TGACGCGCGACGGGATGATCGCGTTCACGCCGCACCCGCGCGCGCTCGGCTCGGCGCTCACGCATCCGTCGCTCACGACC GATTATTCGGAGGCGCTCGTCGAGCTGATCACGCCTGCGGAGCGCGACGCCGCGACGACGCTCGAGCGGCTCGACGAACT GCATCGCTTCGTCTACGCGTCGATCGGCGACGAGATGCTGTGGACGGATTCGATGCCGGGCCTGCTGCCGGCCGACGACG AAATTCCGATCGCCGACTACGGCACGTCGAACATCGGGCGGCTGAAGACCGTTTACCGGCGCGGCCTCGCGTATCGCTAC GGGCGCACGATGCAGTGCATCGCGGGGATTCACTACAACTATTCGCTGAGCGAGGAAGTGTGGCGGCGGCTGCACGCGGA GGAGGGTTCGACCGCGAGCATCGTCGATTACCAGTCGGAGCGCTATCTCGCGCTGATCCGCAATTTCCGTCGCACGAGCT GGCTGCTGATGTACCTGTTCGGCGCGTCGCCCGCGCTCGATCTGGGTTTCCTGCGCGGCCGGGCGCACAAGCTCGACACG TTCGACGCCGCGACGCTGTATCGCCCGCACGCGACGAGCCTGCGGATGAGCGACCTCGGCTACTCGAACACGACTGCGCA GGCCGCGCTGCAGGCCGACTACAACACGCTGTCCGGCTATCTCGACGCGCTGTCGAAGGCGGTCAGCGAGCCGTATCCGC CGTACGAGGCGATCGGCACGCACCGCGACGGCGAGTGGATTCAGATCAACACGAACGTGCTGCAGATCGAGAACGAGTTC TACTCGACGATCCGGCCGAAGCGCGTCACGTATTCGGGCGAGCGGCCGCTTCACGCGCTTGCGTCGCGCGGCGTCCAGTA CATCGAGGTGCGCTGTCTCGACATCGATCCGTTCGAGCCGACGGGCATCGCGCTCGACACCGCGCGCTTTCTCGACGCGT ACCTGCTCGTCTGCGCGCTCGACGAGAGCGCGCCGCTCACGTGCGACGCGTATCGCGAGTCGAATGCGAACTTCGGCCGC GTGACGATGGAGGGGCGCAAGCCGGGGCTCGAGCTCGCGCGCAACGGCGCGGCCGTGCCGATGCGCACATGGGCCGACGA GATCTTCGCGCAGATCGAAGCCGCGGGCCGCGTGCTCGACGACATCCGCGGCGGCGACGCGCATGCGCGCGCGATCGCCG CGCAGCGCGCGAAGCTCGACGATCCGGAGCTCACGTCTTCCGCGCGCGTGCTGCGCGCGATGCGCGAGAACGGGCAGTCG TTCCTCGAGTTCGCACGCGCGCAAAGCGCCGCGCATGCGGCGTACTTCCGCGGCCGCCCGCTCGACGAGGCCGCGACGCG CGATGCGCAGGCGCTCGCCGAGCGTTCGCTCGCCGAGCAGGCCGAGCTCGAAGGCAAGAACGCCGGATCGTTCGACGCGT TCGTCGCCGCGTATCGCGCATATACGTTGAACCGCTTCAGCGTGTGA
Upstream 100 bases:
>100_bases CGCGCGAGTCGTCAGGCAGCGCCGAAGGGCAGGTCAAGCGAGCCTTTCGGCGAGATGTTAAAGTGCCCGCTACTGTCAAA TCCCGCCAGGTGATCCGCGC
Downstream 100 bases:
>100_bases CGAGCGGCGCGGGCGTTACGGCTGCGCGTCGAGGAAGTCGCGCGCGTTGCATGCGTCGGCGGTGATGCGCAGCGTTTGCA GCGCGCGTTTCTCGATGCGG
Product: glutamate--cysteine ligase
Products: NA
Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]
Number of amino acids: Translated: 548; Mature: 547
Protein sequence:
>548_residues MPRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTT DYSEALVELITPAERDAATTLERLDELHRFVYASIGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRY GRTMQCIAGIHYNYSLSEEVWRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEF YSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGR VTMEGRKPGLELARNGAAVPMRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV
Sequences:
>Translated_548_residues MPRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTT DYSEALVELITPAERDAATTLERLDELHRFVYASIGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRY GRTMQCIAGIHYNYSLSEEVWRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEF YSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGR VTMEGRKPGLELARNGAAVPMRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV >Mature_547_residues PRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTD YSEALVELITPAERDAATTLERLDELHRFVYASIGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYG RTMQCIAGIHYNYSLSEEVWRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDTF DAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFY STIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRV TMEGRKPGLELARNGAAVPMRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQSF LEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV
Specific function: Glutathione biosynthesis; first step. [C]
COG id: COG2918
COG function: function code H; Gamma-glutamylcysteine synthetase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1789044, Length=521, Percent_Identity=41.2667946257198, Blast_Score=381, Evalue=1e-107,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR007370 - InterPro: IPR006334 [H]
Pfam domain/function: PF04262 Glu_cys_ligase [H]
EC number: =6.3.2.2 [H]
Molecular weight: Translated: 60941; Mature: 60810
Theoretical pI: Translated: 5.54; Mature: 5.54
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMI CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEE AFTPHPRALGSALTHPSLTTDYSEALVELITPAERDAATTLERLDELHRFVYASIGDEML EECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE WTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLSEEV ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHH WRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGT CCHHHEECCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC HRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEP CCCCCEEEEECCEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCC TGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLELARNGAAVP CCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCHHHHCCCCCCC MRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRA HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH YTLNRFSV HHHHHCCC >Mature Secondary Structure PRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMI CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEE AFTPHPRALGSALTHPSLTTDYSEALVELITPAERDAATTLERLDELHRFVYASIGDEML EECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE WTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLSEEV ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHH WRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGT CCHHHEECCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC HRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEP CCCCCEEEEECCEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCC TGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLELARNGAAVP CCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCHHHHCCCCCCC MRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRA HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH YTLNRFSV HHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA