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The map label for this gene is def-2 [H]

Identifier: 83719851

GI number: 83719851

Start: 2292392

End: 2292925

Strand: Direct

Name: def-2 [H]

Synonym: BTH_I2023

Alternate gene names: 83719851

Gene position: 2292392-2292925 (Clockwise)

Preceding gene: 83720010

Following gene: 161723148

Centisome position: 60.18

GC content: 65.54

Gene sequence:

>534_bases
ATGATTCGCGAGATTCTGAAGATGGGCGATCCGCGTCTGCTGGAAGTCGCCCGGCCGGTGGAGTCGTTCAATACGCCGGA
GTTGCACGCGCTCGTCGCGGACATGTTCGAGACGATGCATCACGCGAACGGCGCGGGACTCGCCGCGCCGCAAATCGGCG
TCGGGCTGCAGGTGATCATCTTCGGCTTCGGCAGCAGCGAGCGCTACCCGGAGGCGCCGCCCGTGCCGGAGACCGTGCTA
GTCAATCCGAGCGTCGAATATCTGCCGCCCGACATGGAGGACGGTTGGGAGGGCTGCCTGTCGGTGCCGGGGCTGCGCGG
CGTCGTGAGCCGCTACCGGCGCGTGCGCTACAGCGGCTTCGACCAGTACGGCGCGAAGCTCGAGCGGATCGCGGAGGGCT
TTCATGCGCGGGTCGTCCAGCATGAGTACGACCACCTGATCGGCAAGCTCTATCCGATGCGGATCACCGATTTCTCGAAG
TTCGGCTTTACCGACGTGCTGTTCCCGGGGCTCGACGCGCAAGCGGACGATTGA

Upstream 100 bases:

>100_bases
CGGGCGCCGACGCGGGCAGCAAGCTCGCGAAAGCCGAGGAGCTCGGCATTCCGGTGCTGGACGAAGCAGGCATGCACAAA
CTTCTGGAGGGCCATGCGCG

Downstream 100 bases:

>100_bases
CGCGGCGGGCGCGCGCGCAGGCGAAGCGGAAAAAATCGAAACGCCCGCGTGCGTTTCCGTGCGAGGTCCGCCGGCGCAGG
CCGGGGCTCAAGGCGCAACG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIREILKMGDPRLLEVARPVESFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVIIFGFGSSERYPEAPPVPETVL
VNPSVEYLPPDMEDGWEGCLSVPGLRGVVSRYRRVRYSGFDQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSK
FGFTDVLFPGLDAQADD

Sequences:

>Translated_177_residues
MIREILKMGDPRLLEVARPVESFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVIIFGFGSSERYPEAPPVPETVL
VNPSVEYLPPDMEDGWEGCLSVPGLRGVVSRYRRVRYSGFDQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSK
FGFTDVLFPGLDAQADD
>Mature_177_residues
MIREILKMGDPRLLEVARPVESFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVIIFGFGSSERYPEAPPVPETVL
VNPSVEYLPPDMEDGWEGCLSVPGLRGVVSRYRRVRYSGFDQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSK
FGFTDVLFPGLDAQADD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=34.8571428571429, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=40.9395973154362, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645728, Length=182, Percent_Identity=33.5164835164835, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=36.6863905325444, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19733; Mature: 19733

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILKMGDPRLLEVARPVESFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVII
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGSSERYPEAPPVPETVLVNPSVEYLPPDMEDGWEGCLSVPGLRGVVSRYRRVRYSGF
EECCCCCCCCCCCCCCCEEEECCCHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
DQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSKFGFTDVLFPGLDAQADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MIREILKMGDPRLLEVARPVESFNTPELHALVADMFETMHHANGAGLAAPQIGVGLQVII
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGSSERYPEAPPVPETVLVNPSVEYLPPDMEDGWEGCLSVPGLRGVVSRYRRVRYSGF
EECCCCCCCCCCCCCCCEEEECCCHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
DQYGAKLERIAEGFHARVVQHEYDHLIGKLYPMRITDFSKFGFTDVLFPGLDAQADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]