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The map label for this gene is hisZ [H]

Identifier: 83719752

GI number: 83719752

Start: 2521480

End: 2522628

Strand: Direct

Name: hisZ [H]

Synonym: BTH_I2244

Alternate gene names: 83719752

Gene position: 2521480-2522628 (Clockwise)

Preceding gene: 83719083

Following gene: 83719766

Centisome position: 66.19

GC content: 69.63

Gene sequence:

>1149_bases
ATGTCGACCTGGTTACTTCCCGAGAATATTGCCGACGTGCTGCCGTCGGAGGCGCGCAAGATCGAGGAGCTGCGCCGCAG
GCTGCTCGACCGCTTTCGTTCGTACGGCTACGAAATGGTCATGCCGCCGCTGCTCGAGTATCTGGAGTCGTTGCTGACGA
GCGGCGGCAACGAGCTGCGCCTGCGCACGTTCAAGCTCGTCGATCAGGTGTCGGGCCGCACGCTCGGCTTGCGCGCCGAC
ATGACGCCGCAGGTCGCGCGCATCGACGCACACCTGCTGAACCGCCAGGGCGTGACGCGCCTCTGCTACGCGGGTCCGGT
GCTGCATACGCGCCCTCGCGGGCTGCACGCGAGTCGCGAGCAGTTGCAGATCGGCGCGGAAATCTACGGGCACGCGGGCC
TCGAGGCCGATCAGGAAATCCAGCAACTGATGCTCGACGCGCTGCATCTGGCTGGTCTGAAGAAAATCCGGCTCGACCTC
TGCCACGCGGGGGTGCTGGCCGCGCTCTTCGCGCGCGATGCGGCCGCGGCCGGGCGCGGCGAGGCGCTCTACGAAGCGCT
CGCGGGCAAGGACGTGCCGCGCCTGAACGAGCTGACCGACGATCTGGGTGCCGATACGCGCGCCGCGCTGCGCGCACTGC
CGCGCCTGTACGGCGACGCGTCGGTGCTCGACGAGGCGCGCCGTCAGTTGCCGGCGCTGCCCGAGATCGCCCGCGCGCTC
GACGATCTCGCGCATCTCGCCTCGCAAGTGAAGGACGCCGAAGTCGCGATCGATCTCGCCGATCTGCGCGGCTACGCGTA
CCACAGCGGCGCGATGTTCGCCGCATACGTCGACGGCGTGCCGAACGCGGTCGCGCATGGCGGCCGCTACGATCACGTCG
GTCAGGCATATGGGCGCGCGCGGCCGGCGACGGGCTTTTCGCTCGATTTGCGCGAAATCGCGCGAATTTCGCCGGTCGAG
GCGCGCGGCGCCGCGATTCTCGCGCCGTGGAAGCAGGATGACGCGTTGCGCGCGGCGGTGGGGGCGCTGCGCGACGCGGG
CGAGGTCGTGATCCAGGCGCTGCCGGGGCACGATCACGTGCTCGACGAATTCGCATGCGATCGCGCGCTTGTCGAGCGTG
ACGGCGCGTGGGTGGTCGAGCCCCGGTGA

Upstream 100 bases:

>100_bases
CGGCGCCGCCCGGCCATCCGCCGGGGCGCCGGGCGCCGAGCCGATTCCAATCCGCGGCGCGCGTCGCGCACGCCGCGTTT
CCTGTCGTAGGACCGCAACG

Downstream 100 bases:

>100_bases
ACGCGCCGCGCCGCCGGGCTTCCGATGCGCGTCCAGCGAGCAATGTCGTTGAAGCGAAACGGAAAAATTCGGAACCGGCG
GCGAACCTAGGTAGAATACG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSTWLLPENIADVLPSEARKIEELRRRLLDRFRSYGYEMVMPPLLEYLESLLTSGGNELRLRTFKLVDQVSGRTLGLRAD
MTPQVARIDAHLLNRQGVTRLCYAGPVLHTRPRGLHASREQLQIGAEIYGHAGLEADQEIQQLMLDALHLAGLKKIRLDL
CHAGVLAALFARDAAAAGRGEALYEALAGKDVPRLNELTDDLGADTRAALRALPRLYGDASVLDEARRQLPALPEIARAL
DDLAHLASQVKDAEVAIDLADLRGYAYHSGAMFAAYVDGVPNAVAHGGRYDHVGQAYGRARPATGFSLDLREIARISPVE
ARGAAILAPWKQDDALRAAVGALRDAGEVVIQALPGHDHVLDEFACDRALVERDGAWVVEPR

Sequences:

>Translated_382_residues
MSTWLLPENIADVLPSEARKIEELRRRLLDRFRSYGYEMVMPPLLEYLESLLTSGGNELRLRTFKLVDQVSGRTLGLRAD
MTPQVARIDAHLLNRQGVTRLCYAGPVLHTRPRGLHASREQLQIGAEIYGHAGLEADQEIQQLMLDALHLAGLKKIRLDL
CHAGVLAALFARDAAAAGRGEALYEALAGKDVPRLNELTDDLGADTRAALRALPRLYGDASVLDEARRQLPALPEIARAL
DDLAHLASQVKDAEVAIDLADLRGYAYHSGAMFAAYVDGVPNAVAHGGRYDHVGQAYGRARPATGFSLDLREIARISPVE
ARGAAILAPWKQDDALRAAVGALRDAGEVVIQALPGHDHVLDEFACDRALVERDGAWVVEPR
>Mature_381_residues
STWLLPENIADVLPSEARKIEELRRRLLDRFRSYGYEMVMPPLLEYLESLLTSGGNELRLRTFKLVDQVSGRTLGLRADM
TPQVARIDAHLLNRQGVTRLCYAGPVLHTRPRGLHASREQLQIGAEIYGHAGLEADQEIQQLMLDALHLAGLKKIRLDLC
HAGVLAALFARDAAAAGRGEALYEALAGKDVPRLNELTDDLGADTRAALRALPRLYGDASVLDEARRQLPALPEIARALD
DLAHLASQVKDAEVAIDLADLRGYAYHSGAMFAAYVDGVPNAVAHGGRYDHVGQAYGRARPATGFSLDLREIARISPVEA
RGAAILAPWKQDDALRAAVGALRDAGEVVIQALPGHDHVLDEFACDRALVERDGAWVVEPR

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41708; Mature: 41577

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWLLPENIADVLPSEARKIEELRRRLLDRFRSYGYEMVMPPLLEYLESLLTSGGNELR
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
LRTFKLVDQVSGRTLGLRADMTPQVARIDAHLLNRQGVTRLCYAGPVLHTRPRGLHASRE
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCHH
QLQIGAEIYGHAGLEADQEIQQLMLDALHLAGLKKIRLDLCHAGVLAALFARDAAAAGRG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EALYEALAGKDVPRLNELTDDLGADTRAALRALPRLYGDASVLDEARRQLPALPEIARAL
HHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
DDLAHLASQVKDAEVAIDLADLRGYAYHSGAMFAAYVDGVPNAVAHGGRYDHVGQAYGRA
HHHHHHHHHHCHHHHEEEHHHHCCHHEECCCCHHHHHCCCCHHHHCCCCCCHHHHHHCCC
RPATGFSLDLREIARISPVEARGAAILAPWKQDDALRAAVGALRDAGEVVIQALPGHDHV
CCCCCCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
LDEFACDRALVERDGAWVVEPR
HHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
STWLLPENIADVLPSEARKIEELRRRLLDRFRSYGYEMVMPPLLEYLESLLTSGGNELR
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
LRTFKLVDQVSGRTLGLRADMTPQVARIDAHLLNRQGVTRLCYAGPVLHTRPRGLHASRE
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCHH
QLQIGAEIYGHAGLEADQEIQQLMLDALHLAGLKKIRLDLCHAGVLAALFARDAAAAGRG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EALYEALAGKDVPRLNELTDDLGADTRAALRALPRLYGDASVLDEARRQLPALPEIARAL
HHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
DDLAHLASQVKDAEVAIDLADLRGYAYHSGAMFAAYVDGVPNAVAHGGRYDHVGQAYGRA
HHHHHHHHHHCHHHHEEEHHHHCCHHEECCCCHHHHHCCCCHHHHCCCCCCHHHHHHCCC
RPATGFSLDLREIARISPVEARGAAILAPWKQDDALRAAVGALRDAGEVVIQALPGHDHV
CCCCCCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
LDEFACDRALVERDGAWVVEPR
HHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA