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The map label for this gene is amiC [H]

Identifier: 83719750

GI number: 83719750

Start: 831305

End: 832849

Strand: Reverse

Name: amiC [H]

Synonym: BTH_I0722

Alternate gene names: 83719750

Gene position: 832849-831305 (Counterclockwise)

Preceding gene: 83720658

Following gene: 83721669

Centisome position: 21.86

GC content: 69.58

Gene sequence:

>1545_bases
ATGTTGATCAAACCGTTCCGCTCGATCGAATCGGCGGCCACCGCGACCCATAACTGGCGACGCCGGCAGATCCTGCGCGC
CGGCGCGTCGACGCTCGTGCTCGGGCTCGCGCTGCCGCGCCTCGCGCACGCGAGCTCCGTGCTCGGCGTGCGCGTGTGGC
CCGCGCGCGACTACACGCGCGTGACGATCGAATCGGATCAGCCGCTGCAGAACACGCAGCAACTGCTGCAAGGGCCGGAC
CGGCTCGTCGTCGACCTGAACGGCCTCGAGCTCGACCAGGCGCTGCGCGACCTCGTGTCGAAGATCGCGCCGAACGATCC
GCAGATCCAGTCGGTGCGGGTCGGCCAGTATCAGCCGCATGTCGTGCGGATGGTGTTCGACCTGAAGGGCTCGGTGAAGC
CGCAGGTGTTCACGCTGCCGCCCGTCGGCACCTACAAGTACCGGCTCGTGTTCGACCTGTATCCGGCCGTCGCGCCCGAC
CCGCTGTCCGACCTGATCGCGCAGACGGAGCGCAAGGAACAGCAGTTGAACGATACGCTGCGCGCGCAGCAGGCGCAGCC
GCCGACGGCCGCGCTCAACGGCCCGACCGCCGCGCCGCCCGCCGCGACCGACAACAGCGATGCGTTCTTCCAGCGCTTCG
CGCAGAACGGCACGCCGCCCGCGCACTCGCCGCGGCCGGCGCCCGCCAATCCGCCCGTCGCGCAGACGGCACCGCCCGCC
GCGCCGCAGCGGCCCGCGGTCAAGCCGCCGCCCGTCGTCGCGCGCAAGAACGACGACGATTCCGGCGACGCCGACACCTA
CGCGTTCACCGCGCCGAAGTCCGGCCGCGGCACCGTGCGCCTGCTGACGGTCGCGATCGACCCGGGCCACGGCGGCGAGG
ACCCGGGCGCGATCGGCGGCGCCGGCACGTACGAGAAGCACATCGCGCTCGACATCGCGAAGAAGCTGCGCGCGAAGATC
GACGCGGCGCCCAACATGCGCGCGATGATGACGCGCGACGCCGACTTCTTCGTCCCGCTGAACGTGCGCGTGCAGAAGGC
GCGGCGCGTCGGCGCGGATCTGTTCGTATCGATTCACGCGGATGCGTTCACGACGCCGTCGGCGCGCGGCTCGTCGGTGT
TCGCGCTGTCGGACCACGGCGCGTCGAGCGCGGCCGCGCGCTGGCTCGCGAACAAGGAGAACTCGTCGGACCTGATCGGC
GGGATCAACATCAAGACGCAGGACGTCTCGGTGAACCGCGCGCTCTTCGACATGTCGACGACCGCGCAGATCCGCGATTC
GCTGCGCTACGGCAATTACGTGCTGCGCGAAGTCGGCGGCATCAACAAGCTGCACAAGGGCTCGGTCGAGCAGGCGGGGT
TCGCGGTGCTGAAAGCGCCCGACATTCCGTCGATCCTCGTCGAGACCGCGTTCATCAGCAATCCGGACGAAGAGCGGCGG
CTCAACGATGACGCGTATCGCGACCAGATGGCCGACGCGATCTTCCGCGGGATCAAGCGCTATTTCGCGGCGAATCCGCC
GCTCGCGAAGAGCCGGATGACGTAA

Upstream 100 bases:

>100_bases
TTGGCGTGCCGGATCTCGTGTTCTCGCTCGACGTGGACGGCGAAGGCCGCCGCCTCACCGCCCGGGCGTACAGCGCATCA
GGAAAGGCATGTCTCGAAAG

Downstream 100 bases:

>100_bases
CCCGCGCGCGCGGCGCGGCGACGCCGTGGCCGCCGCGCCCCGAGTGCCGCCTTCCGGCTCGCGCGAGCGCGGGCCGGCCC
TACCCCGCCATCACGACGCG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLALPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNTQQLLQGPD
RLVVDLNGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQQLNDTLRAQQAQPPTAALNGPTAAPPAATDNSDAFFQRFAQNGTPPAHSPRPAPANPPVAQTAPPA
APQRPAVKPPPVVARKNDDDSGDADTYAFTAPKSGRGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIALDIAKKLRAKI
DAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGASSAAARWLANKENSSDLIG
GINIKTQDVSVNRALFDMSTTAQIRDSLRYGNYVLREVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERR
LNDDAYRDQMADAIFRGIKRYFAANPPLAKSRMT

Sequences:

>Translated_514_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLALPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNTQQLLQGPD
RLVVDLNGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQQLNDTLRAQQAQPPTAALNGPTAAPPAATDNSDAFFQRFAQNGTPPAHSPRPAPANPPVAQTAPPA
APQRPAVKPPPVVARKNDDDSGDADTYAFTAPKSGRGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIALDIAKKLRAKI
DAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGASSAAARWLANKENSSDLIG
GINIKTQDVSVNRALFDMSTTAQIRDSLRYGNYVLREVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERR
LNDDAYRDQMADAIFRGIKRYFAANPPLAKSRMT
>Mature_514_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLALPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNTQQLLQGPD
RLVVDLNGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQQLNDTLRAQQAQPPTAALNGPTAAPPAATDNSDAFFQRFAQNGTPPAHSPRPAPANPPVAQTAPPA
APQRPAVKPPPVVARKNDDDSGDADTYAFTAPKSGRGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIALDIAKKLRAKI
DAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGASSAAARWLANKENSSDLIG
GINIKTQDVSVNRALFDMSTTAQIRDSLRYGNYVLREVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERR
LNDDAYRDQMADAIFRGIKRYFAANPPLAKSRMT

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=264, Percent_Identity=52.6515151515151, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=48.2758620689655, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=41.4847161572052, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 55678; Mature: 55678

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLALPRLAHASSVLGVRVWPARDYTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEE
VTIESDQPLQNTQQLLQGPDRLVVDLNGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPH
EEECCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
VVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQQLNDTL
HEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RAQQAQPPTAALNGPTAAPPAATDNSDAFFQRFAQNGTPPAHSPRPAPANPPVAQTAPPA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
APQRPAVKPPPVVARKNDDDSGDADTYAFTAPKSGRGTVRLLTVAIDPGHGGEDPGAIGG
CCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCC
AGTYEKHIALDIAKKLRAKIDAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHA
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCHHHHHHHHCCCEEEEEEEC
DAFTTPSARGSSVFALSDHGASSAAARWLANKENSSDLIGGINIKTQDVSVNRALFDMST
CCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEEECCCCHHHHHCCCCH
TAQIRDSLRYGNYVLREVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERR
HHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHH
LNDDAYRDQMADAIFRGIKRYFAANPPLAKSRMT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLALPRLAHASSVLGVRVWPARDYTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEE
VTIESDQPLQNTQQLLQGPDRLVVDLNGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPH
EEECCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
VVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQQLNDTL
HEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RAQQAQPPTAALNGPTAAPPAATDNSDAFFQRFAQNGTPPAHSPRPAPANPPVAQTAPPA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
APQRPAVKPPPVVARKNDDDSGDADTYAFTAPKSGRGTVRLLTVAIDPGHGGEDPGAIGG
CCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCC
AGTYEKHIALDIAKKLRAKIDAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHA
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCHHHHHHHHCCCEEEEEEEC
DAFTTPSARGSSVFALSDHGASSAAARWLANKENSSDLIGGINIKTQDVSVNRALFDMST
CCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEEECCCCHHHHHCCCCH
TAQIRDSLRYGNYVLREVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERR
HHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHH
LNDDAYRDQMADAIFRGIKRYFAANPPLAKSRMT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]