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The map label for this gene is ppsA [H]

Identifier: 83719736

GI number: 83719736

Start: 2319503

End: 2321902

Strand: Direct

Name: ppsA [H]

Synonym: BTH_I2046

Alternate gene names: 83719736

Gene position: 2319503-2321902 (Clockwise)

Preceding gene: 83720815

Following gene: 83719889

Centisome position: 60.89

GC content: 64.96

Gene sequence:

>2400_bases
ATGACTAACGCAGCAAACGTCGCAAAGGATCAGGCGTATGTAATTCCGTTCGAGCAGTTGCGAATGACCGATGTCGAAAT
CGTCGGCGGCAAGAATGCGTCGCTCGGCGAGATGATCAGCCAACTGGCCGAGGCAGGCGTGCGCGTGCCCACCGGTTTCG
CAACGACCGCGCTCGCATTCCGCGATTTTCTCCAGCACAACAACCTCACCGAACGCATCGCGCAACGTCTCGAGTCGCTC
GACGTCGACGACGTGAAGGCGCTCGCCGAAGCCGGTGCCGAGATCCGCAAGTGGATCGTCGACGCGCCGCTGCAGCCGCG
CCTCGAGCAGGAAATCCGCTCGCAGTTCGAGATCCTGAAGAACGGCTCGCCGGGCGAGCTGTCGTTCGCCGTGCGCTCGT
CCGCGACCGCGGAAGATCTGCCCGACGCATCGTTCGCCGGTCAGCAGGAGTCGTATCTGAACGTCGTCGGCATCGAGGAT
GTGCTCGACCGGATGAAGCACGTGTTCGCGTCGCTGTACAACGACCGCGCGATCTCGTACCGCGTGCACAAGGGCTTCAC
GCACGCCGAGGTCGCGCTGTCGGCAGGCGTGCAGCGGATGGTGCGCTCGGACGTCGGCGCGGCCGGCGTGATGTTCACGA
TCGACACCGAATCGGGCTTCAAGGACGCCGTGTTCATCACCGCGAGCTACGGCCTGGGCGAAACCGTCGTGCAGGGCGCG
GTGAACCCGGACGAGTTCTATGTGTTCAAGACGACGCTCGAGCAGGACAAGTACCCGATCATCCGCCGCTCGATCGGCTC
GAAGCTGATCAAGATGGAGTTCACGCAGCCGGGCGAGCCGGGCCGCGTGAAGACGGTCGACGTGCCGCACGAGCAGCGCA
ACCGCTATTCGATCACCGACGACGACGTGATCGAGCTCGCGAAGTACGCGGTCATCATCGAGAAGCACTACCAGCGTCCG
ATGGACATCGAGTGGGGCAAGGACGGCCGCGACGGCAAGATCTTCATCCTGCAGGCGCGCCCCGAAACGGTGAAGAGCCA
GGCGCACGGCAAGGTCGAGCAGCGTTTCAAGCTGAAGGGCCAGTCGCAGGTGCTCGCGACGGGCCGCGCGATCGGCCAGA
AGATCGGCGCGGGCCCCGTGCGCGTGATTCACGATCCGTCGGAGATGGAGCGCGTGCAACCGGGCGACGTGCTCGTCGCC
GACATGACCGACCCGAACTGGGAGCCGGTGATGAAGCGCGCGTCCGCGATCGTCACGAACCGCGGCGGCCGCACCTGCCA
CGCGGCGATCATCGCGCGCGAGCTCGGCGTGCCGGCGGTGGTCGGTTGCGGCGACGCGACCGACGTGCTGAAGGACGGCT
CGCTCGTCACCGTGTCGTGCGCGGAAGGCGACGAAGGCAAGATCTACGACGGCCTGCTCGAGACCGAGGTGTCCGAGGTG
CAGCGCGGCGAGCTGCCGAGCGTGCCGGTCAAGATCATGATGAACGTCGGCAACCCGCAACTCGCGTTCGACTTCTCGCA
ACTGCCGAATGCCGGTGTCGGCCTCGCGCGCCTCGAGTTCATCATCAACAACAACATCGGCGTGCATCCGAAGGCGATCC
TCGAGTATCCGAACATCGACGCCGACCTGAAGAAGGCGGTCGAGAGCGTCGCGCGCGGCCACGCGTCGCCGCGCGCGTTC
TACGTGGACAAGCTGACGGAAGGCATCGCGACGATCGCGGCGGCGTTCTATCCGAAGCCCGTGATCGTGCGCCTGTCCGA
CTTCAAGTCGAACGAGTACAAGAAGCTGATCGGCGGCTCGCGCTACGAGCCGGACGAGGAAAACCCGATGCTGGGCTTCC
GCGGCGCATCGCGCTACATCGCCGAGGACTTCGCGCAGGCGTTCGAGATGGAGTGCATGGCGCTCAAGCGCGTTCGCGAC
GAGATGGGCCTGACCAACGTCGAGATCATGGTGCCGTTCGTGCGCACCGTGAAGCAGGCGGAGCGCGTCGTCGGCCTGCT
CGGGAAGTTCGGCCTGAAGCGCGGCGACAACGGCCTGCGCCTCATCATGATGTGCGAAGTGCCGTCGAACGCGATTCTCG
CCGAAGAGTTCCTGCAGCACTTCGACGGCTTCTCGATCGGCTCGAACGACCTGACGCAGCTGACGCTCGGCCTCGATCGC
GACTCGGGCATGGAACTGCTCGCCGTCGACTTCGACGAACGCGATCCGGCCGTGAAGTTCATGTTGAAGCGCGCGATCGA
CACGTGCCGCAAGCTCGACAAGTACGTCGGCATCTGCGGTCAGGGTCCGTCCGATCACCCGGATTTCGCGAAGTGGCTCG
CCGACGAAGGCATCGCTTCGATCTCGCTGAACCCGGACACGGTCATCGAGACGTGGCAGGCGCTCGCCGCGAAGCAGTAA

Upstream 100 bases:

>100_bases
TTATCCGACAGGTTGTGCAATGCGCGTCCCGCTTTTTGCAAGCGGCCGCGCTCCGAGCCCGTTGCACGATCGAGCATTTT
TTTCACACTTAGGGGCTTGT

Downstream 100 bases:

>100_bases
TTCGCGGTAATCGCCGGTCCGGGCGGCGGGCGTCGATCGCCGTCCGGATGAAGGTGAATTGCAAGGTTCGTGCTATAAAC
ACCCCGGTAACGCCGGGGTG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAFRDFLQHNNLTERIAQRLESL
DVDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRSQFEILKNGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIED
VLDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITASYGLGETVVQGA
VNPDEFYVFKTTLEQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDDDVIELAKYAVIIEKHYQRP
MDIEWGKDGRDGKIFILQARPETVKSQAHGKVEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIHDPSEMERVQPGDVLVA
DMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGSLVTVSCAEGDEGKIYDGLLETEVSEV
QRGELPSVPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGHASPRAF
YVDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAQAFEMECMALKRVRD
EMGLTNVEIMVPFVRTVKQAERVVGLLGKFGLKRGDNGLRLIMMCEVPSNAILAEEFLQHFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAVKFMLKRAIDTCRKLDKYVGICGQGPSDHPDFAKWLADEGIASISLNPDTVIETWQALAAKQ

Sequences:

>Translated_799_residues
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAFRDFLQHNNLTERIAQRLESL
DVDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRSQFEILKNGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIED
VLDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITASYGLGETVVQGA
VNPDEFYVFKTTLEQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDDDVIELAKYAVIIEKHYQRP
MDIEWGKDGRDGKIFILQARPETVKSQAHGKVEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIHDPSEMERVQPGDVLVA
DMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGSLVTVSCAEGDEGKIYDGLLETEVSEV
QRGELPSVPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGHASPRAF
YVDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAQAFEMECMALKRVRD
EMGLTNVEIMVPFVRTVKQAERVVGLLGKFGLKRGDNGLRLIMMCEVPSNAILAEEFLQHFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAVKFMLKRAIDTCRKLDKYVGICGQGPSDHPDFAKWLADEGIASISLNPDTVIETWQALAAKQ
>Mature_798_residues
TNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAFRDFLQHNNLTERIAQRLESLD
VDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRSQFEILKNGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIEDV
LDRMKHVFASLYNDRAISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITASYGLGETVVQGAV
NPDEFYVFKTTLEQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITDDDVIELAKYAVIIEKHYQRPM
DIEWGKDGRDGKIFILQARPETVKSQAHGKVEQRFKLKGQSQVLATGRAIGQKIGAGPVRVIHDPSEMERVQPGDVLVAD
MTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGSLVTVSCAEGDEGKIYDGLLETEVSEVQ
RGELPSVPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGHASPRAFY
VDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAQAFEMECMALKRVRDE
MGLTNVEIMVPFVRTVKQAERVVGLLGKFGLKRGDNGLRLIMMCEVPSNAILAEEFLQHFDGFSIGSNDLTQLTLGLDRD
SGMELLAVDFDERDPAVKFMLKRAIDTCRKLDKYVGICGQGPSDHPDFAKWLADEGIASISLNPDTVIETWQALAAKQ

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=784, Percent_Identity=64.7959183673469, Blast_Score=1046, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=399, Percent_Identity=24.5614035087719, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=32.2916666666667, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI48994992, Length=406, Percent_Identity=24.8768472906404, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=31.2236286919831, Blast_Score=100, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87860; Mature: 87729

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAF
CCCCCCCCCCCEEEEEHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RDFLQHNNLTERIAQRLESLDVDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRSQFEILK
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIEDVLDRMKHVFASLYNDRAISY
CCCCCCEEEEEECCCCHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEE
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITASYGLGETVVQGA
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECCCCHHHHHCC
VNPDEFYVFKTTLEQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITD
CCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHCCCCCCCC
DDVIELAKYAVIIEKHYQRPMDIEWGKDGRDGKIFILQARPETVKSQAHGKVEQRFKLKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHCCC
QSQVLATGRAIGQKIGAGPVRVIHDPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTN
CHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
RGGRTCHAAIIARELGVPAVVGCGDATDVLKDGSLVTVSCAEGDEGKIYDGLLETEVSEV
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
QRGELPSVPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNID
HCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCHHEEEEEECCCCCCCCHHHEECCCCC
ADLKKAVESVARGHASPRAFYVDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
RYEPDEENPMLGFRGASRYIAEDFAQAFEMECMALKRVRDEMGLTNVEIMVPFVRTVKQA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
ERVVGLLGKFGLKRGDNGLRLIMMCEVPSNAILAEEFLQHFDGFSIGSNDLTQLTLGLDR
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
DSGMELLAVDFDERDPAVKFMLKRAIDTCRKLDKYVGICGQGPSDHPDFAKWLADEGIAS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEE
ISLNPDTVIETWQALAAKQ
EECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNAANVAKDQAYVIPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAF
CCCCCCCCCCEEEEEHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RDFLQHNNLTERIAQRLESLDVDDVKALAEAGAEIRKWIVDAPLQPRLEQEIRSQFEILK
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NGSPGELSFAVRSSATAEDLPDASFAGQQESYLNVVGIEDVLDRMKHVFASLYNDRAISY
CCCCCCEEEEEECCCCHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEE
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDAVFITASYGLGETVVQGA
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECCCCHHHHHCC
VNPDEFYVFKTTLEQDKYPIIRRSIGSKLIKMEFTQPGEPGRVKTVDVPHEQRNRYSITD
CCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHCCCCCCCC
DDVIELAKYAVIIEKHYQRPMDIEWGKDGRDGKIFILQARPETVKSQAHGKVEQRFKLKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHCCC
QSQVLATGRAIGQKIGAGPVRVIHDPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTN
CHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
RGGRTCHAAIIARELGVPAVVGCGDATDVLKDGSLVTVSCAEGDEGKIYDGLLETEVSEV
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
QRGELPSVPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNID
HCCCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCHHEEEEEECCCCCCCCHHHEECCCCC
ADLKKAVESVARGHASPRAFYVDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
RYEPDEENPMLGFRGASRYIAEDFAQAFEMECMALKRVRDEMGLTNVEIMVPFVRTVKQA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
ERVVGLLGKFGLKRGDNGLRLIMMCEVPSNAILAEEFLQHFDGFSIGSNDLTQLTLGLDR
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
DSGMELLAVDFDERDPAVKFMLKRAIDTCRKLDKYVGICGQGPSDHPDFAKWLADEGIAS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEE
ISLNPDTVIETWQALAAKQ
EECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]