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The map label for this gene is thlA [H]

Identifier: 83719693

GI number: 83719693

Start: 2544338

End: 2545522

Strand: Direct

Name: thlA [H]

Synonym: BTH_I2261

Alternate gene names: 83719693

Gene position: 2544338-2545522 (Clockwise)

Preceding gene: 83718975

Following gene: 83720860

Centisome position: 66.79

GC content: 69.03

Gene sequence:

>1185_bases
ATGCAACGCGAAGTAGTGGTGGTGAGCGGCGTACGCACCGCGATCGGCGATTTCGGCGGCAGCCTGAAGGATTTCGCGCC
GACCGAGCTCGGTGCGCGCGTGGTGCGCGAGGTGTTGTCGCGCGCGCAGGTGTCGGGCGACGAAGTCGGCCACGTCGTGT
TCGGCAACGTCGTGCACACGGAACCGAAGGACATGTATCTCGCGCGCGTCGCGGCGATCAACGGTGGCGTCGCGCAGCAC
GCGCCGGCGCTGACGCTCAACCGGCTGTGCGGCTCGGGCCTGCAGGCGATCGTGTCGGCCGCGCAGGGCGTGCTGCTCGG
CGATGCGGACATCGCGGTCGCGGGCGGCGCGGAGAACATGAGCCGCGCGCCGTATGCGGTGCCGTCCGCGCGCTTCGGCC
AGCGCATGGGCGATGCGAAGCTCGTCGACATGATGATCGGCGCGCTCAACGATCCGTTCCAGTCGATTCACATGGGCGTG
ACGGCGGAGAACGTCGCGGCGAAGTACGGGATCACGCGCGACGCGCAGGACGCGCTCGCGCTCGAATCGCACCGGCGCGC
GTCGCATGCGACGAAGTCCGGCTACTTCAAGGAGCAGATCCTGCCGATCGAGATCGCGTCGCGCAAGGGCGCGGTGGTGT
TCGACGCCGACGAACACGTGCGCCACGACGCGTCGCTCGACGATTTCACGAAGCTCAAGCCCGTGTTCGTCAAGGAGAAC
GGCACGGTGACGGCGGGCAATGCATCGGGCATCAACGATGCGGCCGCCGCGGTCGTGCTGATGGAGCGCAGCGTCGCCGA
GAAGCGCGGCGCGAAGCCGCTCGCGCGCCTCGTCTCGTATGCGCATGCGGGCGTCGATCCGGCGTACATGGGCATCGGTC
CCGTGCCCGCGACGAAGAAGGCGCTCGAGCGCGCGGGCATCACGGTCGCCGATCTCGACGTGATCGAGGCAAACGAGGCG
TTTGCCGCGCAGGCGTGCGCGGTGTCGAACGAGCTCGGCCTCGATCCGGCGAAGGTCAACCCGAACGGCTCGGGCATCTC
GCTCGGTCATCCGATCGGCGCGACGGGCGCGCTCATCACCGTGAAGGCGCTCTACGAGCTGCAGCGCATCGGCGGGCGCT
ACGCGCTCGTCACGATGTGCATCGGCGGCGGGCAGGGTATCGCCGCGGTGTTCGAGCGTCTGTGA

Upstream 100 bases:

>100_bases
ATGCAACGCGCGAATCCGACGCGTGATCCGAATGCACGACGCGCGCGATCGCGCTCGCGCGCGCCATCGGTTTTCATCAA
GAGAAGAAAGGGAGGCAACG

Downstream 100 bases:

>100_bases
CGAGAGGGCCGCGCAGTTGAAGCTGATCACCGGAATCCGCGGCGCGGCGCTCGCCGCCGCGCTCGCGACCGCGACCGGGC
CTGCGTTCGCGCAGTCTTCT

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQH
APALTLNRLCGSGLQAIVSAAQGVLLGDADIAVAGGAENMSRAPYAVPSARFGQRMGDAKLVDMMIGALNDPFQSIHMGV
TAENVAAKYGITRDAQDALALESHRRASHATKSGYFKEQILPIEIASRKGAVVFDADEHVRHDASLDDFTKLKPVFVKEN
GTVTAGNASGINDAAAAVVLMERSVAEKRGAKPLARLVSYAHAGVDPAYMGIGPVPATKKALERAGITVADLDVIEANEA
FAAQACAVSNELGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGIAAVFERL

Sequences:

>Translated_394_residues
MQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQH
APALTLNRLCGSGLQAIVSAAQGVLLGDADIAVAGGAENMSRAPYAVPSARFGQRMGDAKLVDMMIGALNDPFQSIHMGV
TAENVAAKYGITRDAQDALALESHRRASHATKSGYFKEQILPIEIASRKGAVVFDADEHVRHDASLDDFTKLKPVFVKEN
GTVTAGNASGINDAAAAVVLMERSVAEKRGAKPLARLVSYAHAGVDPAYMGIGPVPATKKALERAGITVADLDVIEANEA
FAAQACAVSNELGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGIAAVFERL
>Mature_394_residues
MQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQH
APALTLNRLCGSGLQAIVSAAQGVLLGDADIAVAGGAENMSRAPYAVPSARFGQRMGDAKLVDMMIGALNDPFQSIHMGV
TAENVAAKYGITRDAQDALALESHRRASHATKSGYFKEQILPIEIASRKGAVVFDADEHVRHDASLDDFTKLKPVFVKEN
GTVTAGNASGINDAAAAVVLMERSVAEKRGAKPLARLVSYAHAGVDPAYMGIGPVPATKKALERAGITVADLDVIEANEA
FAAQACAVSNELGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGIAAVFERL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=45.0381679389313, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=45.5242966751918, Blast_Score=351, Evalue=6e-97,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=41.6666666666667, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI4557237, Length=395, Percent_Identity=39.2405063291139, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=33.8823529411765, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=31.4578005115089, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=49.4974874371859, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=46.1734693877551, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=43.8118811881188, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=39.1959798994975, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=35.9413202933985, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=46.0358056265985, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=33.8422391857506, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=398, Percent_Identity=34.1708542713568, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.4964539007092, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=26.9135802469136, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=37.7171215880893, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=36.2282878411911, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=46.6836734693878, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=42.8571428571429, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=40.4580152671756, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=31.2206572769953, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40910; Mature: 40910

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGDEVGHVVFGNVVHT
CCCCEEEECCHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEC
EPKDMYLARVAAINGGVAQHAPALTLNRLCGSGLQAIVSAAQGVLLGDADIAVAGGAENM
CCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEECCCCCC
SRAPYAVPSARFGQRMGDAKLVDMMIGALNDPFQSIHMGVTAENVAAKYGITRDAQDALA
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCHHHHHH
LESHRRASHATKSGYFKEQILPIEIASRKGAVVFDADEHVRHDASLDDFTKLKPVFVKEN
HHHHHHHHHHHHCCCHHHHEEEEEEECCCCCEEECCHHHHHCCCCCCHHHCCCEEEEECC
GTVTAGNASGINDAAAAVVLMERSVAEKRGAKPLARLVSYAHAGVDPAYMGIGPVPATKK
CEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHCCCCCCCHHHH
ALERAGITVADLDVIEANEAFAAQACAVSNELGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEEECEEEECCHHHHHHHHHHHHHCCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRIGGRYALVTMCIGGGQGIAAVFERL
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGDEVGHVVFGNVVHT
CCCCEEEECCHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEC
EPKDMYLARVAAINGGVAQHAPALTLNRLCGSGLQAIVSAAQGVLLGDADIAVAGGAENM
CCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEECCCCCC
SRAPYAVPSARFGQRMGDAKLVDMMIGALNDPFQSIHMGVTAENVAAKYGITRDAQDALA
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCHHHHHH
LESHRRASHATKSGYFKEQILPIEIASRKGAVVFDADEHVRHDASLDDFTKLKPVFVKEN
HHHHHHHHHHHHCCCHHHHEEEEEEECCCCCEEECCHHHHHCCCCCCHHHCCCEEEEECC
GTVTAGNASGINDAAAAVVLMERSVAEKRGAKPLARLVSYAHAGVDPAYMGIGPVPATKK
CEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHCCCCCCCHHHH
ALERAGITVADLDVIEANEAFAAQACAVSNELGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEEECEEEECCHHHHHHHHHHHHHCCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRIGGRYALVTMCIGGGQGIAAVFERL
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]