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The map label for this gene is dmlR [H]

Identifier: 83719671

GI number: 83719671

Start: 2259586

End: 2260569

Strand: Direct

Name: dmlR [H]

Synonym: BTH_I1996

Alternate gene names: 83719671

Gene position: 2259586-2260569 (Clockwise)

Preceding gene: 83720420

Following gene: 83719653

Centisome position: 59.32

GC content: 68.6

Gene sequence:

>984_bases
TTGATCTTTTACTTTTCGATATCTAATCTGGAGCGACTTCAGATTCGACGGGGTGGCCGCGTCGCGCCGCCCGAACATGG
GATGGATCGCTTCAAACAGATCGAAACGTTTGCCGCGGTCGCGGCAAAGGGCAGCCTGTCGGCGGCCGCGCACGCGGAAG
GGGTCGCGCCCGCGATCATCGGTCGCCGGCTCGACGCGCTCGAGGAGCGGCTCGGCGTGAAGCTGCTCGTGCGCACGACG
CGCAAGCTCACGCTGACGTTCGAAGGCTCGGCGTTCCTCGAGGACTGCCAGCGGATCATCAACGACATGCAGAACGCCGA
GGCAAGCGTGTCCGCGGGCGGCGTGAAGGCGAGCGGGCACCTGCGGGTGTCGGCGCCCGCGGGCTTCGGCCGGCGCCACG
TCGCGCCGCTCGTGCCCGCGTTCACGGGCGCGCATCCGGACGTGTCGGTCACGCTCGATCTGTCCGACCGGATGGTCGAC
CTCGTCAACGAAGGGTTCGATTGCGCGGTGCGGCTGGGCGAGCTGCCCGATTCGTCGCTCGTGTCGCTGAAGCTCGGCGA
GAACCGGCGCGTGTGTGTCGCGTCGCCCGCGTATCTCGCGCGCGCGGGCATGCCCGAGACGCTCGACGATCTCGCGCGCC
ACAACTGCCTCGCGCTCGCGGCGAATGCGAACCAGCAGCGCGGCTGGACCTTCGTCGACGATGGCAAGGTGGTGTCGATC
CGCGTGTCCGGCACGATGGAGTGCTCGGACGGCGCGGTGCTGCACGAATGGTGCCTCGAGGGGCATGGCCTGGCCTGGCG
TTCGTGGTGGGAAGTCGGCGCGGACATCGCGGCGGGCCGGCTCGTGAGCGTGCTCGACGCGTTCGCCGCGCCGCCGATCG
GCATTCACGCGGTGTTTCCGCAGCGCCGGCACCTGCCGCTCAGGGTCCGGCTCTTTCTCGATTTTCTGAAGCACACGTAC
GAGCGCCCCGGCTACTGGGGATGA

Upstream 100 bases:

>100_bases
AGGCGGGCGGGGTGCCGGGCATGACGTTGCGCTCCACGATCAGAGGTCGAAGTCGATTCTATTAGTGATGACTTGAACTA
AAAAAGACAGTAATCATCAC

Downstream 100 bases:

>100_bases
CGCCCGCGCGGCGGCGCTCGCGGGCGCCGCATGCGCGGCGTCTTCGCGCGCGGCTTCGGTGCGAGATCCTTGCGCCGGAT
TCGTACCCGAATTCGCGCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIFYFSISNLERLQIRRGGRVAPPEHGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTT
RKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPAFTGAHPDVSVTLDLSDRMVD
LVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPETLDDLARHNCLALAANANQQRGWTFVDDGKVVSI
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTY
ERPGYWG

Sequences:

>Translated_327_residues
MIFYFSISNLERLQIRRGGRVAPPEHGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTT
RKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPAFTGAHPDVSVTLDLSDRMVD
LVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPETLDDLARHNCLALAANANQQRGWTFVDDGKVVSI
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTY
ERPGYWG
>Mature_327_residues
MIFYFSISNLERLQIRRGGRVAPPEHGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTT
RKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPAFTGAHPDVSVTLDLSDRMVD
LVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPETLDDLARHNCLALAANANQQRGWTFVDDGKVVSI
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDFLKHTY
ERPGYWG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=35.8620689655172, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=31.1188811188811, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=33.2203389830508, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=30.6896551724138, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.0353356890459, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.2682926829268, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=28.2828282828283, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=28.8, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786448, Length=277, Percent_Identity=26.7148014440433, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=23.9057239057239, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35610; Mature: 35610

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFYFSISNLERLQIRRGGRVAPPEHGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAII
CEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHH
GRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGH
HHHHHHHHHHHCEEEEEEECEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
LRVSAPAGFGRRHVAPLVPAFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSL
EEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCE
VSLKLGENRRVCVASPAYLARAGMPETLDDLARHNCLALAANANQQRGWTFVDDGKVVSI
EEEEECCCCEEEEECCHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCEEECCCEEEEE
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPIGIHAVFP
EEECEEECCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECC
QRRHLPLRVRLFLDFLKHTYERPGYWG
CCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFYFSISNLERLQIRRGGRVAPPEHGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAII
CEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHH
GRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGH
HHHHHHHHHHHCEEEEEEECEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
LRVSAPAGFGRRHVAPLVPAFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSL
EEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCE
VSLKLGENRRVCVASPAYLARAGMPETLDDLARHNCLALAANANQQRGWTFVDDGKVVSI
EEEEECCCCEEEEECCHHHHHCCCCHHHHHHHHCCEEEEEECCCCCCCCEEECCCEEEEE
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPIGIHAVFP
EEECEEECCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECC
QRRHLPLRVRLFLDFLKHTYERPGYWG
CCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]