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The map label for this gene is hupA [H]

Identifier: 83719600

GI number: 83719600

Start: 5354

End: 5734

Strand: Direct

Name: hupA [H]

Synonym: BTH_I0004

Alternate gene names: 83719600

Gene position: 5354-5734 (Clockwise)

Preceding gene: NA

Following gene: 83720377

Centisome position: 0.14

GC content: 60.37

Gene sequence:

>381_bases
TTGCGCCGTGCCGTTGCCACACACTATAGACGGCACGGTTCGCGCACGTATAATCGGCGCGTTCGCGCTGTTCGTGTCAA
CCTAACCTGGAAAGGAACCTTAATGAACAAACAGGAACTGATCGACGCCGTCGCCGCCCAGACGGGCGCCAGCAAGGCTC
AAACCGGCGAAACGCTGGACACGCTCCTCGAAGTGATCAAGAAGGCTGTGTCGAAAGGCGACTCAGTTCAACTGATCGGC
TTCGGCAGCTTCGGTTCGGGCAAGCGCGCAGCGCGCACCGGCCGTAACCCCAAGACGGGCGAGACCATCAAGATCCCGGC
GGCCAAGACGGTCAAGTTCACGGCTGGCAAGGCGTTCAAGGACGCAGTGAACAAGCGCTGA

Upstream 100 bases:

>100_bases
CCCTTGTCGTTATTGGGTTTGCGCTGCGCTATCATAGCAAGCGCCGTATCGCGGAGCTGCCAGGAGGCAAACCCGGCGGT
GCGTTGCGCGGCGCAATACT

Downstream 100 bases:

>100_bases
AACGAGCTGTTCGCGAACAAAAAACCCGCTGGTTTCAGCGGGTTTTTTCTTTTCGGCGCGTTTTTTTTTGCCGCGCCGCA
TGGCCTTTTTCAATCTTTGA

Product: DNA-binding protein HU-alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MRRAVATHYRRHGSRTYNRRVRAVRVNLTWKGTLMNKQELIDAVAAQTGASKAQTGETLDTLLEVIKKAVSKGDSVQLIG
FGSFGSGKRAARTGRNPKTGETIKIPAAKTVKFTAGKAFKDAVNKR

Sequences:

>Translated_126_residues
MRRAVATHYRRHGSRTYNRRVRAVRVNLTWKGTLMNKQELIDAVAAQTGASKAQTGETLDTLLEVIKKAVSKGDSVQLIG
FGSFGSGKRAARTGRNPKTGETIKIPAAKTVKFTAGKAFKDAVNKR
>Mature_126_residues
MRRAVATHYRRHGSRTYNRRVRAVRVNLTWKGTLMNKQELIDAVAAQTGASKAQTGETLDTLLEVIKKAVSKGDSVQLIG
FGSFGSGKRAARTGRNPKTGETIKIPAAKTVKFTAGKAFKDAVNKR

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=53.3333333333333, Blast_Score=97, Evalue=4e-22,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=50.561797752809, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=37.3626373626374, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=37.0786516853933, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 13743; Mature: 13743

Theoretical pI: Translated: 11.96; Mature: 11.96

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAVATHYRRHGSRTYNRRVRAVRVNLTWKGTLMNKQELIDAVAAQTGASKAQTGETLD
CCCHHHHHHHHCCCCCHHCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
TLLEVIKKAVSKGDSVQLIGFGSFGSGKRAARTGRNPKTGETIKIPAAKTVKFTAGKAFK
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCEEECCCCCEEEECCCHHHH
DAVNKR
HHHCCC
>Mature Secondary Structure
MRRAVATHYRRHGSRTYNRRVRAVRVNLTWKGTLMNKQELIDAVAAQTGASKAQTGETLD
CCCHHHHHHHHCCCCCHHCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
TLLEVIKKAVSKGDSVQLIGFGSFGSGKRAARTGRNPKTGETIKIPAAKTVKFTAGKAFK
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCEEECCCCCEEEECCCHHHH
DAVNKR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]