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The map label for this gene is aes [H]

Identifier: 83719598

GI number: 83719598

Start: 2427897

End: 2428880

Strand: Direct

Name: aes [H]

Synonym: BTH_I2149

Alternate gene names: 83719598

Gene position: 2427897-2428880 (Clockwise)

Preceding gene: 83719036

Following gene: 83720509

Centisome position: 63.74

GC content: 69.41

Gene sequence:

>984_bases
ATGTTTCGCCGTTTGCCCTTGACGATGCCGCTGAACCCGAAGATCGCGCAGGTGCTCGACATGATCGAGCGCGCAAAACG
TCCCGATTATCACGAACAGACGCCCGCGCAGGCGCGCGCGGCGTACGAGAAGAGCGCGCCGATCCTCGACGTCGCGCCCG
CGCCGATGTTTTCGATCGAAGAGCTGCGCGTGCCGTCGCGCGACGGCGGCGCGTTCGGCGCGCGGCTCTATCTGCCCGTC
GAGCCGAGTCTCGCCGAGCCGCTGCCCGCGCTCGTCTACTATCACGGCGGCGGCTTCACGGTCGGCAGCGTGAACACGCA
CGACGCACTGTGCCGGATGTTCGCGCGCGATGCGCATTGCGCGGTGCTGTCGGTCGATTACCGGCTTGCGCCGGAGCACA
AGTTTCCGACGGCGGTCGACGACGCGGAGGACGCGCTCGTGTGGCTGCACGCGCATGCGTCGCGCTTCGGGATCGATCAG
GCGCGGCTCGCGGTCGGCGGCGACAGCGCGGGCGGCACGCTCGCGACGGTGTGCGCGGTGCTCGCGCGCGAGCGCGGGAT
CGCGCTCGCGCTGCAACTGCTGGTCTATCCGGGCACGACGGGGCATCAGCAGACCGCATCGCACGCGCGGCTCGCGAAGG
GTTATTTGCTGTCGGCGGATACGATCCAGTGGTTTTTCGACCATTACGTGCGCGACGCGTCGGATCGCGACGACTGGCGC
TTCGCGCCGCTCGACGGCACGCGCGGCGCGCCGTCGTTCGAGCGCGTCGCGCCCGCGTGGATCGCGACGGCGGAGTACGA
TCCGCTATCCGACGAGGGGGACGCGTACGCGGACAAGCTGCGCGCGGCGGGCAACCGGGTGACGCTTGTCGCGTACGCGG
GGATGATTCACGAGTTTTTCAAGATGGGCGGCTTCGTGCCGGAAGTCAGGCTTGCGCATGCGGACGCGGCGGGCGCGTTG
CGCGCGGCATTCGACGGCGCTTGA

Upstream 100 bases:

>100_bases
TTCGGCAAGCAGCTCGTCGCGCTCGTCTGACGCGTCGTGGCATCATACGGCGCTTGTCCGGGCGCTTTCGCCGGCGCGCT
CGCGCCGCTCGCCTGGCTGT

Downstream 100 bases:

>100_bases
GCGCGGGCGATTCGACGCGGCGCGCAATCCGCGGGACGGGACGTGCGTCATGGGGCGCATGAAATACCGGGCGGCCCGCC
GCGGCGCGTGGCGGCTTGCA

Product: esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFRRLPLTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSIEELRVPSRDGGAFGARLYLPV
EPSLAEPLPALVYYHGGGFTVGSVNTHDALCRMFARDAHCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDQ
ARLAVGGDSAGGTLATVCAVLARERGIALALQLLVYPGTTGHQQTASHARLAKGYLLSADTIQWFFDHYVRDASDRDDWR
FAPLDGTRGAPSFERVAPAWIATAEYDPLSDEGDAYADKLRAAGNRVTLVAYAGMIHEFFKMGGFVPEVRLAHADAAGAL
RAAFDGA

Sequences:

>Translated_327_residues
MFRRLPLTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSIEELRVPSRDGGAFGARLYLPV
EPSLAEPLPALVYYHGGGFTVGSVNTHDALCRMFARDAHCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDQ
ARLAVGGDSAGGTLATVCAVLARERGIALALQLLVYPGTTGHQQTASHARLAKGYLLSADTIQWFFDHYVRDASDRDDWR
FAPLDGTRGAPSFERVAPAWIATAEYDPLSDEGDAYADKLRAAGNRVTLVAYAGMIHEFFKMGGFVPEVRLAHADAAGAL
RAAFDGA
>Mature_327_residues
MFRRLPLTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSIEELRVPSRDGGAFGARLYLPV
EPSLAEPLPALVYYHGGGFTVGSVNTHDALCRMFARDAHCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDQ
ARLAVGGDSAGGTLATVCAVLARERGIALALQLLVYPGTTGHQQTASHARLAKGYLLSADTIQWFFDHYVRDASDRDDWR
FAPLDGTRGAPSFERVAPAWIATAEYDPLSDEGDAYADKLRAAGNRVTLVAYAGMIHEFFKMGGFVPEVRLAHADAAGAL
RAAFDGA

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=273, Percent_Identity=26.7399267399267, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI157041239, Length=110, Percent_Identity=39.0909090909091, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI61966717, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI68299767, Length=302, Percent_Identity=26.4900662251656, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI68051721, Length=276, Percent_Identity=25.7246376811594, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI21328446, Length=122, Percent_Identity=33.6065573770492, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786682, Length=304, Percent_Identity=28.6184210526316, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996133, Length=291, Percent_Identity=27.1477663230241, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=264, Percent_Identity=29.5454545454545, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001146, Length=152, Percent_Identity=37.5, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540028, Length=149, Percent_Identity=36.241610738255, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24656084, Length=102, Percent_Identity=37.2549019607843, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24656076, Length=102, Percent_Identity=37.2549019607843, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130169, Length=102, Percent_Identity=37.2549019607843, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 35501; Mature: 35501

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRLPLTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSIE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHH
ELRVPSRDGGAFGARLYLPVEPSLAEPLPALVYYHGGGFTVGSVNTHDALCRMFARDAHC
HHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCE
AVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDQARLAVGGDSAGGTLATVCAV
EEEEEEEEECCCCCCCCCCCCCCCEEEEEEEHHHHCCCCHHEEEECCCCCCCHHHHHHHH
LARERGIALALQLLVYPGTTGHQQTASHARLAKGYLLSADTIQWFFDHYVRDASDRDDWR
HHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCEEECHHHHHHHHHHHHHCCCCCCCCE
FAPLDGTRGAPSFERVAPAWIATAEYDPLSDEGDAYADKLRAAGNRVTLVAYAGMIHEFF
EECCCCCCCCCCHHHHCCHHEEECCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHH
KMGGFVPEVRLAHADAAGALRAAFDGA
HHCCCCCCCEEECCCHHHHHHHHCCCC
>Mature Secondary Structure
MFRRLPLTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSIE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHH
ELRVPSRDGGAFGARLYLPVEPSLAEPLPALVYYHGGGFTVGSVNTHDALCRMFARDAHC
HHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCE
AVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDQARLAVGGDSAGGTLATVCAV
EEEEEEEEECCCCCCCCCCCCCCCEEEEEEEHHHHCCCCHHEEEECCCCCCCHHHHHHHH
LARERGIALALQLLVYPGTTGHQQTASHARLAKGYLLSADTIQWFFDHYVRDASDRDDWR
HHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCEEECHHHHHHHHHHHHHCCCCCCCCE
FAPLDGTRGAPSFERVAPAWIATAEYDPLSDEGDAYADKLRAAGNRVTLVAYAGMIHEFF
EECCCCCCCCCCHHHHCCHHEEECCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHH
KMGGFVPEVRLAHADAAGALRAAFDGA
HHCCCCCCCEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA