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The map label for this gene is dksA [H]

Identifier: 83719514

GI number: 83719514

Start: 197522

End: 197938

Strand: Reverse

Name: dksA [H]

Synonym: BTH_I0166

Alternate gene names: 83719514

Gene position: 197938-197522 (Counterclockwise)

Preceding gene: 83721300

Following gene: 284159403

Centisome position: 5.2

GC content: 60.91

Gene sequence:

>417_bases
ATGACGAAGAAACTCTTGACCGAAGCCGAAATCCTGAAGATGAGCGACAAGGATTACATGAATGAGGATCAGCTCGCCTT
CTTCAAAAATCGGCTCGAACAATTGCAGGCGGAAATTCTCCGCAATGCGGGCCAGACGACCGAGAATCTGCGCGAAACGG
TGATCGTGCCGGACCCTGCCGATCGCGCGACGATCGAGGAAGAGCACGCGCTCGAACTGCGCACGCGCGATCGCGAACGC
AAGCTTCTCAAGAAGGTCCAGCAGTCGCTCGCCCGCATCGAATCGGGCGACTACGGCTGGTGCGAGGAAACGGGCGAGCC
GATCGGCATCCCGCGTCTGCTCGCACGTCCGACGGCCACGCTGTCGCTCGAGGCGCAGGAGCGCCGCGAGTTGCGTCAGA
AGCTGTTCGGCGACTGA

Upstream 100 bases:

>100_bases
CCCGCCACAGCGAATGGCAAACAATTGCAAGGGCTGTTGCGGGTAATCCCAAAGTGCGGGCAATATCCATGGATTCGCCG
CACATTGAAGAAGCAAGCAG

Downstream 100 bases:

>100_bases
CACTCGCCCGGCGCGGCCCGCCTTCCGCGCCTCACACGCTTTGTCCAAAGCGCGCGGCCTGCCGCGCGCTTTTGTTTTTC
CGCCGCCGCTTCGCTTTCCG

Product: DnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MTKKLLTEAEILKMSDKDYMNEDQLAFFKNRLEQLQAEILRNAGQTTENLRETVIVPDPADRATIEEEHALELRTRDRER
KLLKKVQQSLARIESGDYGWCEETGEPIGIPRLLARPTATLSLEAQERRELRQKLFGD

Sequences:

>Translated_138_residues
MTKKLLTEAEILKMSDKDYMNEDQLAFFKNRLEQLQAEILRNAGQTTENLRETVIVPDPADRATIEEEHALELRTRDRER
KLLKKVQQSLARIESGDYGWCEETGEPIGIPRLLARPTATLSLEAQERRELRQKLFGD
>Mature_137_residues
TKKLLTEAEILKMSDKDYMNEDQLAFFKNRLEQLQAEILRNAGQTTENLRETVIVPDPADRATIEEEHALELRTRDRERK
LLKKVQQSLARIESGDYGWCEETGEPIGIPRLLARPTATLSLEAQERRELRQKLFGD

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=118, Percent_Identity=46.6101694915254, Blast_Score=106, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 16010; Mature: 15879

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLLTEAEILKMSDKDYMNEDQLAFFKNRLEQLQAEILRNAGQTTENLRETVIVPDPA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCC
DRATIEEEHALELRTRDRERKLLKKVQQSLARIESGDYGWCEETGEPIGIPRLLARPTAT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHCCCCC
LSLEAQERRELRQKLFGD
EEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKLLTEAEILKMSDKDYMNEDQLAFFKNRLEQLQAEILRNAGQTTENLRETVIVPDPA
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCC
DRATIEEEHALELRTRDRERKLLKKVQQSLARIESGDYGWCEETGEPIGIPRLLARPTAT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHCCCCC
LSLEAQERRELRQKLFGD
EEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555902 [H]