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The map label for this gene is mrcA [H]

Identifier: 83719498

GI number: 83719498

Start: 3471966

End: 3474359

Strand: Direct

Name: mrcA [H]

Synonym: BTH_I3029

Alternate gene names: 83719498

Gene position: 3471966-3474359 (Clockwise)

Preceding gene: 83720146

Following gene: 83721058

Centisome position: 91.15

GC content: 66.46

Gene sequence:

>2394_bases
ATGCAATCCACGAATCCTACGTCCCCGCCTCCCCCGCCCGAGTCGCGCCGCCGCCCGATGTGGCTGAAGCTCGTGCTCGG
CGTCGCAGGCGTGTGCGTGGCGCTCGCGGTCTGCGTCGGGCTCGTGCTCGGCTACGCGCTCGTCGTCGCGTCGCCGAACA
TGCCGTCGCTGGACGCGCTGACCGACTACCGCCCGAAGGTGCCGCTGCGCATCTACACGGCGGATCACGTGCTGATCGGC
GAATTCGGCGAGGAGCGCCGGGACATCGTCCGTCTGCAGGACGTGCCCGATTCGCTGAAGAAAGCGGTGCTCGCGATCGA
GGATGCGCGCTTCTACGAACACGGCGGCGTCGATCTGACGGGCATCATCCGCGCCGGCATCGTCGCGCTGACGAACGGCC
ACGCGACGCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTCGAGCGAGAAGACATACACGCGC
AAGATCTACGAGATGCTGCTCGCGTACCGGATCGAGCGTGCGCTCACGAAAGATCAGATTCTCGAGGTCTACATGAATCA
GATCTATCTGGGCCAGCGCGCCTATGGCTTCGCCGCCGCCGCGCACGCGTACTTCAACAAGGACCTGAAGGACATCACGC
TCGCCGAGGCCGCGATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCATACAACCCGGTCGCGAATCCGAAACGCGCGAAG
GTCCGCCAGCAATACATCCTGCAGCGGATGCTCGAGCTGAACTTCATCACGCGCGAGCAGTACGACGAGGCGCTCGCCGA
GCCGCTTGTCGTCAAGAGCCCGAGCCGCGAATACAGCGTGCACGCGGAATACGTCGCCGAAATGGTTCGGCAGATGATGT
ATGCGCAATATAAGGACGAGGCGTACACGCGCGGGCTGAACGTCGTCACGACGATCGATTCCGCAGACCAGACGCTCGCG
TACCGCGCGCTGCGAAAGGGCCTGATGACGTACGACCGGCGCCACGGCTATCGCGGCCCCGAAGGCTTCATCGAGCTGCC
CGCGAGCCAGGACGACCGCGAGCAGGCGATCGACGACGCGCTCGTCGACCATCCGGATAACGGCGAGTTGGTCGCGGCCG
TCGTCACCGAAACCGGCCCGCGACAGATTCGCGTCGTGTTCTCGAACGGCAGCGACGCGACGGTGAGCGGCGACGATCTG
CGCTTCGCCGCAAGCTCGCTGTCGGCGAGCGCGCAGCCGAACAAGCGGATCCGCCCCGGCGCGATCGTGCGCGTGTTCAA
GAACGACGACGGCTCGTGGGCGATCGGGCAACTGCCGCAGATCGAGGGCGCGTTCATTTCGATCGTTCCGCAGGACGGCG
CGATCCGCTCACTGATCGGCGGGTTCGACTTCAACAAGAACAAGTTCAACCACGTGACGCAGGCGTGGCGGCAGCCGGGT
TCGTCGTTCAAGCCGTTCATTTATTCGGCGTCGCTCGACAAGGGGCTCGGCCCCGCGACGATCATCAACGACGGCCCGCT
CTATTTCAGCGCCGCCGAGACGGGCGGCCAGCCGTGGGAGCCGAAAAACTACGGCGGCGGCTTCGAGGGCCCGATGTCGA
TGCGCGTCGCGCTGCAGCGCTCGCGCAACCTGGTATCGATTCGCATCCTGAATCACATCGGCCCGAAATACGCGCAGCAA
TACATCACGCGCTTCGGCTTCGACGCGGATCGCCACCCGGCCTATCTGCCGATGGCGCTCGGCGCGGGCCTCGTCACGCC
GCTGCAGATGGCGGGCGCGTTCTCGGTGTTCGCCAACGGCGGCTACCGGGTCAATCCGTATCTGATCGCCGAAGTCACCG
ATCCGAGCGGCAACGTCGTCGCGCGCGCGCAGCCGCTCGTCGCCGAGCAGAACGCGCCGCGCGCGATCGACGCGCGCAAC
GCGTACGTGATGAACAGCCTGTTGCAAAGCGTCGCGCAACGCGGCACCGGCGCGCGCTCGAACGTGCTGAAACGCACCGA
CCTGGCCGGCAAGACCGGCACGACGAACGACTCGCGCGACGCATGGTTCGGCGGCTATCAGCACACGCTCGCGGCGATCG
CGTGGATCGGCTACGACAACCCGCGCAGCCTCGGCGATCGCGAAACCGGCGGCGGCCTCGCGCTGCCGGTGTGGATCGAC
TACATGGGCCCCGCGCTCAAGAATGTGCCGGAATACAAAATGCCGATGCCCGACGGCGTCACGACGCTCGGCGGCGAGTT
GTACTTCGACCAGTTCACGCCGGGCCGCGGCTTCGTCTCGACAGTCGGCGTGAACCTGCCGGCCGCGCCGCCCGATTCCG
CGAGCGCCGTGCCCGGGCATGTCGACGAGCAGGAGAAGCAGGACATCATGAATCTGTTCCGCGGCGGCAACTGA

Upstream 100 bases:

>100_bases
TCGGCGCGCCGCCAGGCCGCGCCGGTTCCCGCCCGGAACCCTTCCGGCGTCCGGCAAAACCGGGCAGCTATAATCGCGGG
ACTGTTTTTCTGGTGTGTCT

Downstream 100 bases:

>100_bases
GCATCGTTCGCGCGCCACAGCGAAAAAGCGGCCTTTCTGGCCGCTTTTTTCATATGGGGCTCGATCAGGCTGCGATCGCG
TCCGTGTGACACACGCTCAC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MQSTNPTSPPPPPESRRRPMWLKLVLGVAGVCVALAVCVGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIG
EFGEERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTR
KIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAK
VRQQYILQRMLELNFITREQYDEALAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLA
YRALRKGLMTYDRRHGYRGPEGFIELPASQDDREQAIDDALVDHPDNGELVAAVVTETGPRQIRVVFSNGSDATVSGDDL
RFAASSLSASAQPNKRIRPGAIVRVFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPG
SSFKPFIYSASLDKGLGPATIINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQ
YITRFGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANGGYRVNPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDARN
AYVMNSLLQSVAQRGTGARSNVLKRTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVWID
YMGPALKNVPEYKMPMPDGVTTLGGELYFDQFTPGRGFVSTVGVNLPAAPPDSASAVPGHVDEQEKQDIMNLFRGGN

Sequences:

>Translated_797_residues
MQSTNPTSPPPPPESRRRPMWLKLVLGVAGVCVALAVCVGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIG
EFGEERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTR
KIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAK
VRQQYILQRMLELNFITREQYDEALAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLA
YRALRKGLMTYDRRHGYRGPEGFIELPASQDDREQAIDDALVDHPDNGELVAAVVTETGPRQIRVVFSNGSDATVSGDDL
RFAASSLSASAQPNKRIRPGAIVRVFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPG
SSFKPFIYSASLDKGLGPATIINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQ
YITRFGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANGGYRVNPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDARN
AYVMNSLLQSVAQRGTGARSNVLKRTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVWID
YMGPALKNVPEYKMPMPDGVTTLGGELYFDQFTPGRGFVSTVGVNLPAAPPDSASAVPGHVDEQEKQDIMNLFRGGN
>Mature_797_residues
MQSTNPTSPPPPPESRRRPMWLKLVLGVAGVCVALAVCVGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIG
EFGEERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTR
KIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAK
VRQQYILQRMLELNFITREQYDEALAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLA
YRALRKGLMTYDRRHGYRGPEGFIELPASQDDREQAIDDALVDHPDNGELVAAVVTETGPRQIRVVFSNGSDATVSGDDL
RFAASSLSASAQPNKRIRPGAIVRVFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPG
SSFKPFIYSASLDKGLGPATIINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQ
YITRFGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANGGYRVNPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDARN
AYVMNSLLQSVAQRGTGARSNVLKRTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVWID
YMGPALKNVPEYKMPMPDGVTTLGGELYFDQFTPGRGFVSTVGVNLPAAPPDSASAVPGHVDEQEKQDIMNLFRGGN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=805, Percent_Identity=38.2608695652174, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=38.1526104417671, Blast_Score=149, Evalue=9e-37,
Organism=Escherichia coli, GI1788867, Length=228, Percent_Identity=32.8947368421053, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789601, Length=178, Percent_Identity=34.2696629213483, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 87168; Mature: 87168

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTNPTSPPPPPESRRRPMWLKLVLGVAGVCVALAVCVGLVLGYALVVASPNMPSLDAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TDYRPKVPLRIYTADHVLIGEFGEERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLT
HCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
GIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQI
HHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVYMNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAK
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
VRQQYILQRMLELNFITREQYDEALAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHH
AYTRGLNVVTTIDSADQTLAYRALRKGLMTYDRRHGYRGPEGFIELPASQDDREQAIDDA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHH
LVDHPDNGELVAAVVTETGPRQIRVVFSNGSDATVSGDDLRFAASSLSASAQPNKRIRPG
HHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEECCCCHHHHHHHHCCCCCCCCCCCCC
AIVRVFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPG
EEEEEEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
SSFKPFIYSASLDKGLGPATIINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQR
CCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRNLVSIRILNHIGPKYAQQYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANG
CCCEEEEEEHHHCCHHHHHHHHHHHCCCCCCCCCEEHHHHCCCHHHHHHHHHHHEEEECC
GYRVNPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDARNAYVMNSLLQSVAQRGTGARS
CEEECCEEEEEEECCCCCEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
NVLKRTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVWID
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEHHHH
YMGPALKNVPEYKMPMPDGVTTLGGELYFDQFTPGRGFVSTVGVNLPAAPPDSASAVPGH
HHCHHHHCCCCCCCCCCCCCHHCCCEEEEECCCCCCCCHHEECCCCCCCCCCCCCCCCCC
VDEQEKQDIMNLFRGGN
CCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSTNPTSPPPPPESRRRPMWLKLVLGVAGVCVALAVCVGLVLGYALVVASPNMPSLDAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TDYRPKVPLRIYTADHVLIGEFGEERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLT
HCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
GIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQI
HHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVYMNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAK
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
VRQQYILQRMLELNFITREQYDEALAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHH
AYTRGLNVVTTIDSADQTLAYRALRKGLMTYDRRHGYRGPEGFIELPASQDDREQAIDDA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHH
LVDHPDNGELVAAVVTETGPRQIRVVFSNGSDATVSGDDLRFAASSLSASAQPNKRIRPG
HHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEECCCCHHHHHHHHCCCCCCCCCCCCC
AIVRVFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPG
EEEEEEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
SSFKPFIYSASLDKGLGPATIINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQR
CCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRNLVSIRILNHIGPKYAQQYITRFGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANG
CCCEEEEEEHHHCCHHHHHHHHHHHCCCCCCCCCEEHHHHCCCHHHHHHHHHHHEEEECC
GYRVNPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDARNAYVMNSLLQSVAQRGTGARS
CEEECCEEEEEEECCCCCEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
NVLKRTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDNPRSLGDRETGGGLALPVWID
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEHHHH
YMGPALKNVPEYKMPMPDGVTTLGGELYFDQFTPGRGFVSTVGVNLPAAPPDSASAVPGH
HHCHHHHCCCCCCCCCCCCCHHCCCEEEEECCCCCCCCHHEECCCCCCCCCCCCCCCCCC
VDEQEKQDIMNLFRGGN
CCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA