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The map label for this gene is rlmI [H]

Identifier: 83719434

GI number: 83719434

Start: 199711

End: 200982

Strand: Reverse

Name: rlmI [H]

Synonym: BTH_I0169

Alternate gene names: 83719434

Gene position: 200982-199711 (Counterclockwise)

Preceding gene: 83720176

Following gene: 83719451

Centisome position: 5.28

GC content: 70.52

Gene sequence:

>1272_bases
ATGCATACCGTTACGCTCAAGCCGTCGAAAGACAAATCCCTGCTGCGCCGCCATCCGTGGGTCTACGCGAACGCGATCGA
CCGCGTCGACGGCAAGCCCGCGCCCGGCGCGACCGTCATCGTGCGCGCGCACGACGGGCGCTTCCTCGCGCGCGCCGCCT
ACAGCCCGCATTCGCAGATCCGGCTGCGCGTATGGAGCTTCGACGAGAACGAGCCGATCGACCATGCGTTCTTCAAGCGG
CGCGTGCAGCGCGCGGTCGCGCATCGCCGCGCGATGATCGCGGGCACGGGCGCGGTGCGGCTCGTGTTCGGCGAGGCGGA
CGGGCTGCCGGGGCTCATCGTCGATCACTACGTCGCCGCGCCGATCCCCGCCGGCGACGCAGCCGCGTGCGCGGCCGAAG
GCGGTGCGACGCAGCAGGCCGAGCCTGCCGAAGGCGCGGGCCGCGGCCAGCTCGTCTGCCAGTTCATGGCGGCGGGCGTC
GAGCACTGGAAGGACGCGATCGTCGCGGCGCTCGTCGCGGCCACGGGCTGCCCGAACGTCTACGAACGCTCGGACGTGTC
GATCCGCGAGAAGGAAGGGCTCGAGCAGACGACGGGCGTGCTCGCGGGCGACGCGCCGCCCGACACGCTGATCGCGAACG
AGAACGGCGTGCTGTATCACGTCGACGTGCGCAACGGCCACAAGACGGGCTTCTACGTCGACCAGCGCGAGAACCGCGCG
CTCGTCGCGCAGTACGCGCGCGATCGCGACGTGCTGAACTGCTTCTGCTACACGGGCGGCTTCTCGCTCGCGGCGCTCAA
GGGCGGCGCGAAGCGGGTCGTGTCGATCGATTCGTCGGGCGACGCGCTCGCGCTCGCGCAGCGCAACGTCGCCGCGAACG
GCTTCGACGCCGCGCGCGCGCAATGGCTCGACGCCGACGCGTTCAAGACGCTGCGCCGCCTCGTCGACGAAGGCGAGCGC
TTCGACCTGATCGTGCTCGATCCGCCGAAGTTCGCGCCGACGCGCGACAGCGTCGATCGCGCGGCGCGCGCGTACAAGGA
CATCAACCTGAGCGGCTTCAAGCTGCTGCGTCCGGGCGGCCTGCTGTTCACGTACTCGTGCTCGGGCGCGATCGACATGG
ACCTGTTCCAGAAGATCGTCGCGGGCGCGGCGGCCGACGCGAAGGTCGACGCGCGCATCCTCAAGCGGCTCGGCGCGGGC
GTCGACCACCCGCTCCTGACCGCCTTCCCCGAAGGCGAATATCTGAAGGGGCTGCTGTTGCAAATCGCGTGA

Upstream 100 bases:

>100_bases
GCGAAAAAGCGCGACTGACGCGCGCCGCCGCGCTCGCCGAATCGCTCTGAATCAACGAATCACATTCCGCCGCGCCCCGC
GCGGCCATTTCGATCCCGAC

Downstream 100 bases:

>100_bases
TCGCCCCGATCTGTGCGGGCGGCGCTGAGCGAGCCCGCGCCAGGCCGGCCGTCCGGCCGACAGCGGGGGCTTGACAGATA
TGTTTCAATAAATGGCTGAG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MHTVTLKPSKDKSLLRRHPWVYANAIDRVDGKPAPGATVIVRAHDGRFLARAAYSPHSQIRLRVWSFDENEPIDHAFFKR
RVQRAVAHRRAMIAGTGAVRLVFGEADGLPGLIVDHYVAAPIPAGDAAACAAEGGATQQAEPAEGAGRGQLVCQFMAAGV
EHWKDAIVAALVAATGCPNVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQRENRA
LVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARAQWLDADAFKTLRRLVDEGER
FDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFKLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAG
VDHPLLTAFPEGEYLKGLLLQIA

Sequences:

>Translated_423_residues
MHTVTLKPSKDKSLLRRHPWVYANAIDRVDGKPAPGATVIVRAHDGRFLARAAYSPHSQIRLRVWSFDENEPIDHAFFKR
RVQRAVAHRRAMIAGTGAVRLVFGEADGLPGLIVDHYVAAPIPAGDAAACAAEGGATQQAEPAEGAGRGQLVCQFMAAGV
EHWKDAIVAALVAATGCPNVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQRENRA
LVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARAQWLDADAFKTLRRLVDEGER
FDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFKLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAG
VDHPLLTAFPEGEYLKGLLLQIA
>Mature_423_residues
MHTVTLKPSKDKSLLRRHPWVYANAIDRVDGKPAPGATVIVRAHDGRFLARAAYSPHSQIRLRVWSFDENEPIDHAFFKR
RVQRAVAHRRAMIAGTGAVRLVFGEADGLPGLIVDHYVAAPIPAGDAAACAAEGGATQQAEPAEGAGRGQLVCQFMAAGV
EHWKDAIVAALVAATGCPNVYERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQRENRA
LVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARAQWLDADAFKTLRRLVDEGER
FDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFKLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAG
VDHPLLTAFPEGEYLKGLLLQIA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=417, Percent_Identity=43.6450839328537, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1787181, Length=213, Percent_Identity=29.1079812206573, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45549; Mature: 45549

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTVTLKPSKDKSLLRRHPWVYANAIDRVDGKPAPGATVIVRAHDGRFLARAAYSPHSQI
CCEEECCCCCCHHHHHHCCCEEECHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCEE
RLRVWSFDENEPIDHAFFKRRVQRAVAHRRAMIAGTGAVRLVFGEADGLPGLIVDHYVAA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCCHHHHHHHHC
PIPAGDAAACAAEGGATQQAEPAEGAGRGQLVCQFMAAGVEHWKDAIVAALVAATGCPNV
CCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQRENRA
CCCCCCCCHHHCCCHHHHCEEECCCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCCCH
LVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARA
HHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCHHHH
QWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFKLLRPGG
HHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCC
LLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLTAFPEGEYLKGLLL
EEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHH
QIA
CCC
>Mature Secondary Structure
MHTVTLKPSKDKSLLRRHPWVYANAIDRVDGKPAPGATVIVRAHDGRFLARAAYSPHSQI
CCEEECCCCCCHHHHHHCCCEEECHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCEE
RLRVWSFDENEPIDHAFFKRRVQRAVAHRRAMIAGTGAVRLVFGEADGLPGLIVDHYVAA
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCCHHHHHHHHC
PIPAGDAAACAAEGGATQQAEPAEGAGRGQLVCQFMAAGVEHWKDAIVAALVAATGCPNV
CCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YERSDVSIREKEGLEQTTGVLAGDAPPDTLIANENGVLYHVDVRNGHKTGFYVDQRENRA
CCCCCCCCHHHCCCHHHHCEEECCCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCCCH
LVAQYARDRDVLNCFCYTGGFSLAALKGGAKRVVSIDSSGDALALAQRNVAANGFDAARA
HHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCHHHH
QWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFKLLRPGG
HHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCC
LLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLTAFPEGEYLKGLLL
EEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHH
QIA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA