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The map label for this gene is rumA [H]

Identifier: 83719402

GI number: 83719402

Start: 2505596

End: 2507092

Strand: Direct

Name: rumA [H]

Synonym: BTH_I2229

Alternate gene names: 83719402

Gene position: 2505596-2507092 (Clockwise)

Preceding gene: 83720902

Following gene: 83719421

Centisome position: 65.78

GC content: 66.13

Gene sequence:

>1497_bases
ATGTTCCAACTGATGAGTCGCATCGTCGAATCTCCGTTGCATGGCGGCCGTTCGTCTACAATCTCGCCTTTCCCCACAAT
CGTTCGTCAGGAAAAGCTGGTGTCAGAAGCCGTCCCTCTCTCCACACCCGGCGCGTCGCATGCGGGCGCCGCAACCGATC
GCGCCCCGGTTCTCGATATCGATTCGCTCGACATGGAAGCGCGCGGCGTCGGCCGTGCCGTCGACGAAAACGGCGAACCC
GGCAAGGTGATTTTCGTCGAGGGCGCGTTGCCCGGCGAACGCGTCACCTATTCGAGTTTCCGCCGCAAGCCGACCTACGA
ACAGGCGCAGGTCGTCGATATTCTGCGCCCGAGCGTGATGCGCACGCGGCCGAAATGCGCATTTTTCGGCACATGCGGCG
GCTGTTCGATGCAGCATCTCGACATGCGCGCGCAGGTCGCGATCAAGCAGCGCGTGCTCGAGGACAACCTGTGGCATCTC
GCGAAGCTGCGCGCCGAGGCGATGTTCGCGCCGATTCACGGCCCGTCGTGGGGCTACCGCTATCGTGCCCGCCTCACCGT
GCGCCACGTCGCGAAGAAGGGCGGCGTGCTCGTCGGCTTCCACGAGAAGAAGAGCAGCTACGTCGCCGACATGACGAGCT
GCGAGGTGCTGCCGCCGCACGTGTCCGCGATGCTGGTGCCGCTGCGCCGGCTCGTCGAGCAATTGTCGATCCGCGATCGC
ATGCCGCAGATCGAGCTTGCGGTCGGCTCGCAGGTGACGGCGCTCGTGCTGCGCGTGCTCGAGCCGATCAACGCGGCCGA
CGAGACGCTCCTGCGCGAATTCGCGGACGCGCATCGCGTGCAGTTCTGGCTGCAGCCGAAAGGGCCGGACACGGTCGCGC
CGTTCTATCCGCTCGACGTGCAGCTCGACTACACGCTGCCCGAATTCGGGATCCGGATGCCGTTCAAGCCGACCGATTTC
ACGCAGGTCAACCACCAGATCAACCGCGTGCTCGTCGGTCGGGCGCTGCGCCTGCTCGCGCCCGAGCGCGGCGACCGCGT
GCTCGATCTGTTCTGCGGGATCGGCAACTTCACGCTGCCGCTCGCGCGGCTCGCTCGCGAGGTGGTCGGGATCGAGGGCA
GCGAGACGCTGACGTCGCGCGCGCTCGCGAATGCAAAGGAAAACGGCGTCGACGGGCATACGTCGTTCGCGAGCCGCAAT
CTGTTCGAGGTGACGGCCGACGATCTGCACGCGCTCGGCGCATTCGACAAATTCCTGGTCGATCCGCCGCGCGAAGGCGC
GCTCGCGGTGTCGAAGGCGCTCGCCGAGATCGCGCAGAGCGGCGAAGGGCCGCTGCCCGCGCGGATCGTCTACGTGTCGT
GCAATCCGTCGACGCTCGCACGAGACGCGGGTCTCCTCGTGCACGAGGCAGGTTATCGGCTGAAGGGCGCGGGCGTCGTC
AACATGTTCCCGCACACGTCGCATGTCGAATCGATCGCGCTCTTCGAGCGTGACTGA

Upstream 100 bases:

>100_bases
GACTTCCTGCATGCATAGCACGTCGAAATCGCCGAGGCCGCGCGCGGCGTCGATAGTGCGCGCGAGGTCGACCGTGCCGT
TCGCGTCCCGTCCCCATTGG

Downstream 100 bases:

>100_bases
CGCGAGATCCGGGCAGCGCGCACATAAAAAAACCGGCCCGAGGGCCGGTTTTTTCGTGATGCGCGCAGACGTGCGTGCCG
CCGGGCTTCAGTTGCGGTTG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MFQLMSRIVESPLHGGRSSTISPFPTIVRQEKLVSEAVPLSTPGASHAGAATDRAPVLDIDSLDMEARGVGRAVDENGEP
GKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVMRTRPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHL
AKLRAEAMFAPIHGPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDR
MPQIELAVGSQVTALVLRVLEPINAADETLLREFADAHRVQFWLQPKGPDTVAPFYPLDVQLDYTLPEFGIRMPFKPTDF
TQVNHQINRVLVGRALRLLAPERGDRVLDLFCGIGNFTLPLARLAREVVGIEGSETLTSRALANAKENGVDGHTSFASRN
LFEVTADDLHALGAFDKFLVDPPREGALAVSKALAEIAQSGEGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVV
NMFPHTSHVESIALFERD

Sequences:

>Translated_498_residues
MFQLMSRIVESPLHGGRSSTISPFPTIVRQEKLVSEAVPLSTPGASHAGAATDRAPVLDIDSLDMEARGVGRAVDENGEP
GKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVMRTRPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHL
AKLRAEAMFAPIHGPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDR
MPQIELAVGSQVTALVLRVLEPINAADETLLREFADAHRVQFWLQPKGPDTVAPFYPLDVQLDYTLPEFGIRMPFKPTDF
TQVNHQINRVLVGRALRLLAPERGDRVLDLFCGIGNFTLPLARLAREVVGIEGSETLTSRALANAKENGVDGHTSFASRN
LFEVTADDLHALGAFDKFLVDPPREGALAVSKALAEIAQSGEGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVV
NMFPHTSHVESIALFERD
>Mature_498_residues
MFQLMSRIVESPLHGGRSSTISPFPTIVRQEKLVSEAVPLSTPGASHAGAATDRAPVLDIDSLDMEARGVGRAVDENGEP
GKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVMRTRPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHL
AKLRAEAMFAPIHGPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDR
MPQIELAVGSQVTALVLRVLEPINAADETLLREFADAHRVQFWLQPKGPDTVAPFYPLDVQLDYTLPEFGIRMPFKPTDF
TQVNHQINRVLVGRALRLLAPERGDRVLDLFCGIGNFTLPLARLAREVVGIEGSETLTSRALANAKENGVDGHTSFASRN
LFEVTADDLHALGAFDKFLVDPPREGALAVSKALAEIAQSGEGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVV
NMFPHTSHVESIALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=199, Percent_Identity=30.6532663316583, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI51173878, Length=199, Percent_Identity=30.6532663316583, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=36.5470852017937, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1787083, Length=402, Percent_Identity=24.6268656716418, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790403, Length=359, Percent_Identity=23.6768802228412, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666579, Length=211, Percent_Identity=32.2274881516588, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 54675; Mature: 54675

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLMSRIVESPLHGGRSSTISPFPTIVRQEKLVSEAVPLSTPGASHAGAATDRAPVLDI
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEC
DSLDMEARGVGRAVDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVM
CCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHH
RTRPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYR
HCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEE
YRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDR
EEEEHHHHHHHHHCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
MPQIELAVGSQVTALVLRVLEPINAADETLLREFADAHRVQFWLQPKGPDTVAPFYPLDV
CCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEE
QLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPERGDRVLDLFCGIGNFTLP
EEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHH
LARLAREVVGIEGSETLTSRALANAKENGVDGHTSFASRNLFEVTADDLHALGAFDKFLV
HHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCHHHHCCCEEEECHHHHHHHHHHHHHHC
DPPREGALAVSKALAEIAQSGEGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVV
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCEEEECCCCEEECCCEE
NMFPHTSHVESIALFERD
EECCCCCCHHHEEEEECC
>Mature Secondary Structure
MFQLMSRIVESPLHGGRSSTISPFPTIVRQEKLVSEAVPLSTPGASHAGAATDRAPVLDI
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEC
DSLDMEARGVGRAVDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVM
CCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHH
RTRPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYR
HCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEE
YRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDR
EEEEHHHHHHHHHCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
MPQIELAVGSQVTALVLRVLEPINAADETLLREFADAHRVQFWLQPKGPDTVAPFYPLDV
CCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEE
QLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPERGDRVLDLFCGIGNFTLP
EEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHH
LARLAREVVGIEGSETLTSRALANAKENGVDGHTSFASRNLFEVTADDLHALGAFDKFLV
HHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCHHHHCCCEEEECHHHHHHHHHHHHHHC
DPPREGALAVSKALAEIAQSGEGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVV
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCEEEECCCCEEECCCEE
NMFPHTSHVESIALFERD
EECCCCCCHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA