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The map label for this gene is ilvE-1 [H]

Identifier: 83719372

GI number: 83719372

Start: 757771

End: 758694

Strand: Reverse

Name: ilvE-1 [H]

Synonym: BTH_I0660

Alternate gene names: 83719372

Gene position: 758694-757771 (Counterclockwise)

Preceding gene: 83719737

Following gene: 83721399

Centisome position: 19.92

GC content: 65.04

Gene sequence:

>924_bases
ATGTCAATGGCCGATCGCGACGGCAAGATTTGGATGGACGGCAAGCTGATCGAATGGCGCGACGCCAAGATCCACGTGCT
GACCCACACGCTGCACTACGGCATGGGCGTCTTCGAGGGCGTGCGCGCGTACAAGACGGCCGACGGCGGCACGGCGATCT
TCCGCCTGAAAGAGCACACGAAGCGCCTGCTGAACTCGGCGAAGATCTTCCAGATGGACGTGCCGTTCGACCAGGAAACG
CTCGAGGCCGCGCAGCGCGACGTCGTGCGCGAGAACAAGCTCGAGTCGTGCTACCTGCGCCCGATCATCTGGATCGGCTC
GGAGAAGCTCGGCGTGTCGGCCAAGGGCAACACGATCCACGTCGCGATCGCCGCGTGGCCGTGGGGCGCGTACCTTGGCG
AGGAAGGCCTCGCGAAGGGCATCCGCGTGAAGACCTCGTCGTTCACGCGCCATCACGTGAACGTGTCGATGGTGCGCGCG
AAGGCGTCCGGCTGGTACGTGAATTCGATCCTCGCGAACCAGGAAGCGACGGCCGACGGCTACGACGAGGCGCTGCTGCT
CGACGTCGACGGCTATGTGTCCGAAGGCTCCGGCGAGAACTTCTTCCTCGTGAACCGCGGCAAGCTCTACACGCCGGATC
TCGCGTCGTGCCTCGACGGCATCACGCGCGACACGGTCATCACGCTCGCGAAGGAAGCCGGCATCGAAGTGATCGAGAAG
CGCATCACGCGCGACGAGGTCTACACGGCCGACGAGGCGTTCTTCACCGGCACCGCGGCCGAAGTCACGCCGATCCGCGA
GCTCGACAACCGCACGATCGGCAGCGGCGCGCGCGGCCCGATCACCGAAAAGCTGCAAAGCGCGTTCTTCGACGTCGTCA
ACGGCAAGAGCGCGAAGCACGCGGACTGGCTCACGAAGATCTGA

Upstream 100 bases:

>100_bases
CACGGACAAAAACGCTAAAATGAGCGTCTTTGCGACGCGGAGCCGTTTCGAGCCCCGCTCGCCCCTTCTGCGCATCCCCG
CATCCACTAGGAGAAGACCT

Downstream 100 bases:

>100_bases
GCCCGAATGCGGCGCGGCGCCACGCCGGCGCGCGCCGCTCGCCATCGCCATAACGAGAAAGCCCCATGAGTGAAATCAAG
GAAATGCCGCTCATCGAACT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLKEHTKRLLNSAKIFQMDVPFDQET
LEAAQRDVVRENKLESCYLRPIIWIGSEKLGVSAKGNTIHVAIAAWPWGAYLGEEGLAKGIRVKTSSFTRHHVNVSMVRA
KASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLASCLDGITRDTVITLAKEAGIEVIEK
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPITEKLQSAFFDVVNGKSAKHADWLTKI

Sequences:

>Translated_307_residues
MSMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLKEHTKRLLNSAKIFQMDVPFDQET
LEAAQRDVVRENKLESCYLRPIIWIGSEKLGVSAKGNTIHVAIAAWPWGAYLGEEGLAKGIRVKTSSFTRHHVNVSMVRA
KASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLASCLDGITRDTVITLAKEAGIEVIEK
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPITEKLQSAFFDVVNGKSAKHADWLTKI
>Mature_306_residues
SMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLKEHTKRLLNSAKIFQMDVPFDQETL
EAAQRDVVRENKLESCYLRPIIWIGSEKLGVSAKGNTIHVAIAAWPWGAYLGEEGLAKGIRVKTSSFTRHHVNVSMVRAK
ASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLASCLDGITRDTVITLAKEAGIEVIEKR
ITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPITEKLQSAFFDVVNGKSAKHADWLTKI

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=335, Percent_Identity=27.1641791044776, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI48994963, Length=300, Percent_Identity=47, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17565728, Length=280, Percent_Identity=27.5, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322608, Length=327, Percent_Identity=26.605504587156, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322002, Length=263, Percent_Identity=27.7566539923954, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24641779, Length=286, Percent_Identity=25.8741258741259, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34016; Mature: 33885

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLKEHT
CCCCCCCCEEEECCEEEEECCCEEEEEEEHHHHCCHHHHHHHHEECCCCCEEEEEEHHHH
KRLLNSAKIFQMDVPFDQETLEAAQRDVVRENKLESCYLRPIIWIGSEKLGVSAKGNTIH
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECEEEEECCCCCCEEECCCEEE
VAIAAWPWGAYLGEEGLAKGIRVKTSSFTRHHVNVSMVRAKASGWYVNSILANQEATADG
EEEEECCCHHHHCCHHHHCCEEEEECCCEEEEEEEEEEEEECCCEEEEHHHCCCCCCCCC
YDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLASCLDGITRDTVITLAKEAGIEVIEK
CCCEEEEEECCCEECCCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHCHHHHHH
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPITEKLQSAFFDVVNGKSAKH
HHHHHHHEECCCCEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
ADWLTKI
CHHHHCC
>Mature Secondary Structure 
SMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLKEHT
CCCCCCCEEEECCEEEEECCCEEEEEEEHHHHCCHHHHHHHHEECCCCCEEEEEEHHHH
KRLLNSAKIFQMDVPFDQETLEAAQRDVVRENKLESCYLRPIIWIGSEKLGVSAKGNTIH
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECEEEEECCCCCCEEECCCEEE
VAIAAWPWGAYLGEEGLAKGIRVKTSSFTRHHVNVSMVRAKASGWYVNSILANQEATADG
EEEEECCCHHHHCCHHHHCCEEEEECCCEEEEEEEEEEEEECCCEEEEHHHCCCCCCCCC
YDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLASCLDGITRDTVITLAKEAGIEVIEK
CCCEEEEEECCCEECCCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHCHHHHHH
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPITEKLQSAFFDVVNGKSAKH
HHHHHHHEECCCCEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
ADWLTKI
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]