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The map label for this gene is alkJ [H]

Identifier: 83719292

GI number: 83719292

Start: 226893

End: 228539

Strand: Direct

Name: alkJ [H]

Synonym: BTH_I0194

Alternate gene names: 83719292

Gene position: 226893-228539 (Clockwise)

Preceding gene: 83720416

Following gene: 83719579

Centisome position: 5.96

GC content: 68.85

Gene sequence:

>1647_bases
ATGCAGTACGACTACATTATCGTTGGAGCCGGCTCGGGCGGCTCGAGCCTCGCGGGGCGTCTGGCCGACGCGTGCCCGGA
TGCGACGATCGCGCTGATCGAAGCGGGCGGTCATACCGAACGCAATCTGCTCGTCAACATGCCGGTGGGGATCGCCGCGC
TCGTGCCGTTCAAGCTGGGCACGAACTACGGCTACGAAACGGTGCCGCAGCCGGGCCTCGGCGGGCGCCGCGGCTATCAG
CCCCGCGGCCGCGGGCTCGGCGGCTCGAGCGCGATCAACGCGATGATCTACACGCGCGGCCATCCGCTCGACTACGACGA
GTGGGAGCAGCTCGGCTGCACCGGCTGGGGATGGCGCGACGTGCTGCCGTACTTTCGCCGCGCCGAAGGCAACGAGCGCG
GCGCGAACGAATGGCACGGCGCGGACGGCCCGCTCACGGTGTCCGATCTGCGCTTCCGTAATCCGTTCTCCGAACGATTC
ATCGCGGCCGCGCACGAGGCCGGCTATCCGCTGAACGACGACTTCAACGGCGAGCGCCAGGAAGGCGTCGGCTTCTACCA
GGTCACGCATCGCGACGGCTCGCGCTGCAGCGTCGCGCGTGCGTACGTGTACGGCCGCACGCGGCCGAACCTGCACGTGA
TCGTCGATGCGACGGTGCTGCGCGTCGTGTTCGACGGCAAGCGCGCCACGGGCGTCGAGCTTGCGCGCGGCGGGCGCGTC
GAAAAGCTCGATGCGCGCGCGGAAGTGATTTTGTCCGCCGGCGCGTTCAATACGCCGCAATTGCTGATGTGCTCGGGCGT
CGGCCCCGCCGCGCAACTGCGCCGCCACGGCATCGCGCTCGTGCACGACGCGCCCGATGTCGGCCAGAACCTGATCGATC
ACATCGATTTCATCGTCAACAAGCGCGTGAATTCGTCGGAGCTCGTCGGCATCTGCTTGCGCGGGATCGCGAAGATGACG
CCCGCGCTGTTCAGCTATCTGTCCAGGCGCCGCGGGATGATGACGAGCAACGTCGCCGAGGCGGGCGGCTTCATCAAGAG
CGCGCCCGGACTCGACCGTCCCGATCTGCAATTGCACTTCTGCACCGCGCTTGTCGACGACCACAACCGCAACATGCACT
GGGGCTTCGGCTATTCGCTTCACGTGTGCGCGTTGCGGCCGAAGAGCCGCGGCAACGTCGCGCTCGCGAGCAGCGACGCG
CGCGTCGCGCCGCTCATCGATCCGCGCTTTTTCAGCGACGAACGCGATCTCGATCTGCTCGTCACGGGCGCGAAGGCGAT
GCGCAGGATTCTGTCGGCCGCGTCGCTCGCGTCGCAGGGCGGGCGCGAACTGTATACCGATCCGGGCGACACCGACGCGC
AGCTGCGCGCCGCGATCGTCGCGCATGCGGACACGATCTACCACCCGGTCGGCACGTGCCGGATGGGCGCCGACGCGCGC
GCGGTCGTCGATCCGCAATTGCGCGTGAAAGGGGTGGACGGGCTGCGGATCGTCGATGCGTCGGTGATGCCGACGCTGAT
CGGCGGCAACACGAATGCGCCGACCGTGATGATCGGCGAGCGCGCGGCGGATTTCATCGTGGCCGCGCGCAACGGGCAGG
CCGTGCCCGCGCCTCGCGAGCGGCTCGCGGCGGCGCACGGCGGCTGA

Upstream 100 bases:

>100_bases
TTTGCGTTCCTAAAATGCGCGAACATTACAAGCCTGATCCGGCTGTCTCGATTGCGATTCCAGGCTCCGTTGCGCGGATG
ACAAAACAGGGAGACGTGAC

Downstream 100 bases:

>100_bases
TGCGCGCCGCCGCGCGGGACGTCGCGGGCGCGGCCGTCAGGCGAACGTGTCGACCATCCCGACGCTGCGTCGTGTCACGC
CGCCGGAAGAGACCGCCGAG

Product: GMC family oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MQYDYIIVGAGSGGSSLAGRLADACPDATIALIEAGGHTERNLLVNMPVGIAALVPFKLGTNYGYETVPQPGLGGRRGYQ
PRGRGLGGSSAINAMIYTRGHPLDYDEWEQLGCTGWGWRDVLPYFRRAEGNERGANEWHGADGPLTVSDLRFRNPFSERF
IAAAHEAGYPLNDDFNGERQEGVGFYQVTHRDGSRCSVARAYVYGRTRPNLHVIVDATVLRVVFDGKRATGVELARGGRV
EKLDARAEVILSAGAFNTPQLLMCSGVGPAAQLRRHGIALVHDAPDVGQNLIDHIDFIVNKRVNSSELVGICLRGIAKMT
PALFSYLSRRRGMMTSNVAEAGGFIKSAPGLDRPDLQLHFCTALVDDHNRNMHWGFGYSLHVCALRPKSRGNVALASSDA
RVAPLIDPRFFSDERDLDLLVTGAKAMRRILSAASLASQGGRELYTDPGDTDAQLRAAIVAHADTIYHPVGTCRMGADAR
AVVDPQLRVKGVDGLRIVDASVMPTLIGGNTNAPTVMIGERAADFIVAARNGQAVPAPRERLAAAHGG

Sequences:

>Translated_548_residues
MQYDYIIVGAGSGGSSLAGRLADACPDATIALIEAGGHTERNLLVNMPVGIAALVPFKLGTNYGYETVPQPGLGGRRGYQ
PRGRGLGGSSAINAMIYTRGHPLDYDEWEQLGCTGWGWRDVLPYFRRAEGNERGANEWHGADGPLTVSDLRFRNPFSERF
IAAAHEAGYPLNDDFNGERQEGVGFYQVTHRDGSRCSVARAYVYGRTRPNLHVIVDATVLRVVFDGKRATGVELARGGRV
EKLDARAEVILSAGAFNTPQLLMCSGVGPAAQLRRHGIALVHDAPDVGQNLIDHIDFIVNKRVNSSELVGICLRGIAKMT
PALFSYLSRRRGMMTSNVAEAGGFIKSAPGLDRPDLQLHFCTALVDDHNRNMHWGFGYSLHVCALRPKSRGNVALASSDA
RVAPLIDPRFFSDERDLDLLVTGAKAMRRILSAASLASQGGRELYTDPGDTDAQLRAAIVAHADTIYHPVGTCRMGADAR
AVVDPQLRVKGVDGLRIVDASVMPTLIGGNTNAPTVMIGERAADFIVAARNGQAVPAPRERLAAAHGG
>Mature_548_residues
MQYDYIIVGAGSGGSSLAGRLADACPDATIALIEAGGHTERNLLVNMPVGIAALVPFKLGTNYGYETVPQPGLGGRRGYQ
PRGRGLGGSSAINAMIYTRGHPLDYDEWEQLGCTGWGWRDVLPYFRRAEGNERGANEWHGADGPLTVSDLRFRNPFSERF
IAAAHEAGYPLNDDFNGERQEGVGFYQVTHRDGSRCSVARAYVYGRTRPNLHVIVDATVLRVVFDGKRATGVELARGGRV
EKLDARAEVILSAGAFNTPQLLMCSGVGPAAQLRRHGIALVHDAPDVGQNLIDHIDFIVNKRVNSSELVGICLRGIAKMT
PALFSYLSRRRGMMTSNVAEAGGFIKSAPGLDRPDLQLHFCTALVDDHNRNMHWGFGYSLHVCALRPKSRGNVALASSDA
RVAPLIDPRFFSDERDLDLLVTGAKAMRRILSAASLASQGGRELYTDPGDTDAQLRAAIVAHADTIYHPVGTCRMGADAR
AVVDPQLRVKGVDGLRIVDASVMPTLIGGNTNAPTVMIGERAADFIVAARNGQAVPAPRERLAAAHGG

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=537, Percent_Identity=41.8994413407821, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI1786503, Length=547, Percent_Identity=40.5850091407678, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17532301, Length=549, Percent_Identity=38.0692167577413, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI45549471, Length=573, Percent_Identity=37.347294938918, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI45551458, Length=572, Percent_Identity=37.2377622377622, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24642042, Length=573, Percent_Identity=38.2198952879581, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24642048, Length=579, Percent_Identity=36.9602763385147, Blast_Score=318, Evalue=8e-87,
Organism=Drosophila melanogaster, GI24642059, Length=575, Percent_Identity=35.8260869565217, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24642037, Length=575, Percent_Identity=36.8695652173913, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24642055, Length=585, Percent_Identity=34.3589743589744, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24642039, Length=579, Percent_Identity=37.4784110535406, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24642035, Length=582, Percent_Identity=36.0824742268041, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24650267, Length=576, Percent_Identity=35.5902777777778, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI17137792, Length=564, Percent_Identity=32.9787234042553, Blast_Score=278, Evalue=7e-75,
Organism=Drosophila melanogaster, GI18859995, Length=579, Percent_Identity=35.5785837651123, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI18859993, Length=580, Percent_Identity=32.5862068965517, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=30.1393728222997, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24645930, Length=580, Percent_Identity=29.6551724137931, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24642057, Length=590, Percent_Identity=30.1694915254237, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58896; Mature: 58896

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYDYIIVGAGSGGSSLAGRLADACPDATIALIEAGGHTERNLLVNMPVGIAALVPFKLG
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEEEEEEC
TNYGYETVPQPGLGGRRGYQPRGRGLGGSSAINAMIYTRGHPLDYDEWEQLGCTGWGWRD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHCCCCCCCHHH
VLPYFRRAEGNERGANEWHGADGPLTVSDLRFRNPFSERFIAAAHEAGYPLNDDFNGERQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
EGVGFYQVTHRDGSRCSVARAYVYGRTRPNLHVIVDATVLRVVFDGKRATGVELARGGRV
HCCEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCHHCCCCE
EKLDARAEVILSAGAFNTPQLLMCSGVGPAAQLRRHGIALVHDAPDVGQNLIDHIDFIVN
EECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
KRVNSSELVGICLRGIAKMTPALFSYLSRRRGMMTSNVAEAGGFIKSAPGLDRPDLQLHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCCCCCCEEEE
CTALVDDHNRNMHWGFGYSLHVCALRPKSRGNVALASSDARVAPLIDPRFFSDERDLDLL
EEEECCCCCCCEEEECCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEE
VTGAKAMRRILSAASLASQGGRELYTDPGDTDAQLRAAIVAHADTIYHPVGTCRMGADAR
EECHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCC
AVVDPQLRVKGVDGLRIVDASVMPTLIGGNTNAPTVMIGERAADFIVAARNGQAVPAPRE
EEECCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCHH
RLAAAHGG
HHHHCCCC
>Mature Secondary Structure
MQYDYIIVGAGSGGSSLAGRLADACPDATIALIEAGGHTERNLLVNMPVGIAALVPFKLG
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEEEEEEC
TNYGYETVPQPGLGGRRGYQPRGRGLGGSSAINAMIYTRGHPLDYDEWEQLGCTGWGWRD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHCCCCCCCHHH
VLPYFRRAEGNERGANEWHGADGPLTVSDLRFRNPFSERFIAAAHEAGYPLNDDFNGERQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
EGVGFYQVTHRDGSRCSVARAYVYGRTRPNLHVIVDATVLRVVFDGKRATGVELARGGRV
HCCEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCHHCCCCE
EKLDARAEVILSAGAFNTPQLLMCSGVGPAAQLRRHGIALVHDAPDVGQNLIDHIDFIVN
EECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
KRVNSSELVGICLRGIAKMTPALFSYLSRRRGMMTSNVAEAGGFIKSAPGLDRPDLQLHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCCCCCCEEEE
CTALVDDHNRNMHWGFGYSLHVCALRPKSRGNVALASSDARVAPLIDPRFFSDERDLDLL
EEEECCCCCCCEEEECCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEE
VTGAKAMRRILSAASLASQGGRELYTDPGDTDAQLRAAIVAHADTIYHPVGTCRMGADAR
EECHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCC
AVVDPQLRVKGVDGLRIVDASVMPTLIGGNTNAPTVMIGERAADFIVAARNGQAVPAPRE
EEECCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCHH
RLAAAHGG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]