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The map label for this gene is radA [H]

Identifier: 83719278

GI number: 83719278

Start: 2272805

End: 2274181

Strand: Direct

Name: radA [H]

Synonym: BTH_I2008

Alternate gene names: 83719278

Gene position: 2272805-2274181 (Clockwise)

Preceding gene: 83720249

Following gene: 83718894

Centisome position: 59.67

GC content: 68.12

Gene sequence:

>1377_bases
ATGGCGAAGCAGAAGACGGTTTTCGTCTGCACCGAGTGCGGCGGGCAGACGCCGAAGTGGCAGGGGCAATGCCCGTCGTG
CCAAGCATGGAACACTCTCGTCGAATCGGTGGAGAGCGCGCCGTCCGCGCACCGCTTCCAGTCGCTCGCCAAGCGCGCGC
CCGTGCAGCGGCTCGCCGACATCGAGGCGGCCGACGTGCCGCGCTTCTCGACCGGGATCGGCGAATTCGACCGGGTGCTG
GGCGGCGGGCTCGTCGCGGGCGGGGTCGTGCTGATCGGCGGCGATCCGGGGATTGGCAAGTCGACGCTGCTGTTGCAGTC
GCTCGCGCAGATCGCGAGCGAGCGGCCGGCGCTCTATATCAGCGGCGAGGAATCGGGCGCGCAGATCGCGCTGCGCGCGC
AGCGGCTCGCGCTTCTCGAAGGCGGCGCGAGCGCGGCCGACCTGAAGCTCCTCGCCGAGATCCAGCTCGAGAAAATCCAG
GCGACGATCGACGCCGAGCGGCCCGACGTCGCGGTCATCGACTCGATCCAGACGATCTACTCGGAGGCGCTGACGTCGGC
GCCCGGCTCGGTCGCGCAAGTGCGCGAATGCGCGGCGCAATTGACGCGCATCGCGAAGCAATCGGGCACGGCGATCATCA
TGGTCGGCCACGTGACGAAAGAGGGCAATCTCGCGGGGCCGCGCGTGCTCGAGCACATCGTCGACACCGTGCTGTACTTC
GAAGGCGATACGCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAACTCGGCGTGTT
CGCAATGACCGAGCGCGGCTTGCGCGGCGTCGCGAATCCGTCCGCGCTGTTCCTGTCGCAGCACACGGAGGTCGTGCCGG
GCTCGTGCGTGCTCGTCACGCAGGAGGGCACGCGGCCGCTGCTCGTCGAGGTGCAGGCGCTTGTCGATACCGCGAACGTG
CCGAATCCGCGCCGGCTCGCGGTCGGTCTCGAACAGAACCGGCTCGCGATGCTGCTCGCCGTGCTGCACCGGCATGCAGG
CATCGCGTGCTTCGATCAGGACGTGTTCCTGAACGCGGTCGGCGGCGTGAAGATCACCGAGCCTGCCGCCGATCTCGCGG
TGCTGCTCGCGATCCACTCGTCGATGCGTAACAAACCGTTGCCAAAGGGTCTGATCGTATTCGGCGAAGTCGGACTCGCG
GGCGAGATCCGGCCGTCGCCGCGCGGGCAGGAGCGCCTGCGCGAGGCCGCGAAGCTTGGCTTCACGACCGCGCTGATTCC
GAAGGCGAATGCGCCGAAACAGCCGATCGACGGGCTTCGCGTGCACGCAGTCGAGCGCATCGAGCAGGCGATCGACCAGA
TCCGCGTGCTCGAATGA

Upstream 100 bases:

>100_bases
CCGATCGACGACGTCGCCGCGCGCTGCATGACGGTCGGCTACGAGCTGATGTGCGCGGTCGCGCCGCGCGTGCCGGTGCG
TGCCGAGTAAGGGCGCGCGC

Downstream 100 bases:

>100_bases
GCGCGGCGCTCGGCGGGCGTGTTCCGGCCCGCCGCCTTTCACGCGCGGTCATGCGCGGATCGGGGACGTAAAACCATGTA
ACGTTTTGGTACGCTGATGT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAKQKTVFVCTECGGQTPKWQGQCPSCQAWNTLVESVESAPSAHRFQSLAKRAPVQRLADIEAADVPRFSTGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYISGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQ
ATIDAERPDVAVIDSIQTIYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVTQEGTRPLLVEVQALVDTANV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE

Sequences:

>Translated_458_residues
MAKQKTVFVCTECGGQTPKWQGQCPSCQAWNTLVESVESAPSAHRFQSLAKRAPVQRLADIEAADVPRFSTGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYISGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQ
ATIDAERPDVAVIDSIQTIYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVTQEGTRPLLVEVQALVDTANV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE
>Mature_457_residues
AKQKTVFVCTECGGQTPKWQGQCPSCQAWNTLVESVESAPSAHRFQSLAKRAPVQRLADIEAADVPRFSTGIGEFDRVLG
GGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYISGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQA
TIDAERPDVAVIDSIQTIYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYFE
GDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVTQEGTRPLLVEVQALVDTANVP
NPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLAG
EIRPSPRGQERLREAAKLGFTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=459, Percent_Identity=57.7342047930283, Blast_Score=530, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48736; Mature: 48605

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKTVFVCTECGGQTPKWQGQCPSCQAWNTLVESVESAPSAHRFQSLAKRAPVQRLAD
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHC
IEAADVPRFSTGIGEFDRVLGGGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYI
CCHHCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
SGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQATIDAERPDVAVIDSIQTIY
ECCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
SEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHEEE
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVT
CCCCCHHHHHHHHHHHHCCCHHHCCHHEEHHHCCCCCCCCHHEEEECCCCCCCCCEEEEE
QEGTRPLLVEVQALVDTANVPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAV
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCHHEEEC
GGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLAGEIRPSPRGQERLREAAKLG
CCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
FTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKQKTVFVCTECGGQTPKWQGQCPSCQAWNTLVESVESAPSAHRFQSLAKRAPVQRLAD
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHC
IEAADVPRFSTGIGEFDRVLGGGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYI
CCHHCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
SGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQATIDAERPDVAVIDSIQTIY
ECCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
SEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHEEE
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVT
CCCCCHHHHHHHHHHHHCCCHHHCCHHEEHHHCCCCCCCCHHEEEECCCCCCCCCEEEEE
QEGTRPLLVEVQALVDTANVPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAV
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCHHEEEC
GGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLAGEIRPSPRGQERLREAAKLG
CCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
FTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]