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The map label for this gene is alkJ [H]

Identifier: 83719215

GI number: 83719215

Start: 3794919

End: 3796604

Strand: Reverse

Name: alkJ [H]

Synonym: BTH_I3332

Alternate gene names: 83719215

Gene position: 3796604-3794919 (Counterclockwise)

Preceding gene: 83718489

Following gene: 83720739

Centisome position: 99.67

GC content: 68.98

Gene sequence:

>1686_bases
GTGGCTACCGAACGTACGCTCGAGGGCGAATTCGATTATGTGATCGTCGGCGCCGGCACGGCCGGCTGCGTGCTCGCGAA
CCGGCTCACCGAAGATCCGGACGTGACCGTGCTGCTGCTCGAAGCCGGCGGCAAGGACGACTATCACTGGATTCACATCC
CGGTCGGCTATCTTTACTGCATCGGCAATCCGCGCACCGACTGGCTCTACAAGACCGAGCCCGAAGCGGGCCTGAACGGC
CGCGCGCTGTCGTATCCGCGCGGGCGCGTGCTGGGCGGCTCGTCTTCGATCAACGGCATGATCTACATGCGCGGCCAGCG
CGGCGATTACGACGATTGGGCGCACGCGACGGGCGACGCGGGCTGGTCGTGGGACAGTGTGCTGCCGATCTTCAGGCGCA
GCGAGCATCACCACGCGGGCGCGACCGACGTGCATGGCGCGGGCGGCATGTGGCGCGTCGAGAAGCAGCGGCTGCGCTGG
GAGATTCTCGAGGCGTTCTCGCAGGCCGCGCAGCAAACCGGCATTCCGGCCACCGACGATTTCAATCGCGGCGACAACAC
GGGCGTCGGCTATTTCGAAGTCAATCAGAAGCGCGGCGTCCGCTGGAACGCGTCGAAGGCGTTCCTGCGGCCCGCGCTTG
CGCGGCCGAATCTCACCGTGATCACCGGCGCGCAGGCCGAGCGGCTTGTGTTCGACGGCAAGCGCTGCGCGGGCGTCGAG
TATCTCGGCGGCGGCGCGCCTTTTCTCGCGCGTGCGCGCATCGAAGTGCTGGTCGCGTCGGGCGCGGTGAATTCGCCGCA
ACTGCTCGAATTGTCCGGCATCGGCGACGGCAGCCGGCTGCAGGCGCTCGGCATCGGCGTTGTCGCGGATTTGCGCGGCG
TCGGCGAAAACCTTCAGGATCACTTGCAGTTGCGCATGGCGTTTCGCGTGCGCGGCGTGCGTACGCTGAACACGCTGTCC
GCGCACTGGTGGGGCAAGCTGTGGATCGGCGCGCAGTACGCGTTGATGCAGCGCGGGCCGATGTCGATGGCGCCGTCGCA
GTTGGGCGCGTTCGCGAAATCGGACCCGAACGATCCGGCGCTCACGCGGCCCGATCTCGAATACCACGTGCAGCCGCTGT
CGCTCGAGCGCTTCGGCGAGCCGCTGCATCGCTTCAACGCGTTCACCGCGTCCGTCTGCCATCTGCGGCCGACGTCGCGC
GGCAGCATTCATGCGGTGTCGCCGGACCCGGCGCGCGCGCCGGCGATCGCGCCGAATTATCTGTCGACCGATCACGATCG
ACATGTCGCGGCGAACGCGCTGCGCCTCACGCGCCGGATCGCGGCGGCGCCCGCGCTCGCACGCTATGCGCCCGAGGAAA
TCCTGCCGGGCGCCCAGTATCTGAGCGAAGCGGAGCTGATCGCCGCGGCGGGCGCCGTCGGCACGACGATCTTTCATCCG
GTCGGCACGTGCCGGATGGGCCGCGCGGACGATCCGGACGCGGTTGTCGACAGCCGCCTGCGCGTGCGCGGCGTGACGGG
GCTGCGGATCGTCGATGCATCGGTGATGCCGACGATCACGTCGGGCAACACGAACTCGCCGACGCTGATGATCGCCGAGC
GCGCGAGCGACATGATCCGCGCGGATCGACGCGGCGCACCGGAGCGCGGCGCGGCCGCGAGCGTCGAGGCCGCGCTGCCG
ACGTAA

Upstream 100 bases:

>100_bases
ACGGCGCGCATCGGCGTTCGATGCGACGCTTGGCGCCGAGGGCGCACGACGGCGGTCCGCGCGAACGATCGCCGCAACCC
AATTGAGGAGACGGCAGACC

Downstream 100 bases:

>100_bases
AAGTGACACGCTCCGTTGAAAGAATCGGCAAAGGATCGGCAAAGGATCGGAATGAAAATGCAGCCGAGGGTTAACCCAAA
AGCGAAGCGCGCGATCTCGC

Product: GMC family oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MATERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNG
RALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWAHATGDAGWSWDSVLPIFRRSEHHHAGATDVHGAGGMWRVEKQRLRW
EILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
YLGGGAPFLARARIEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVVADLRGVGENLQDHLQLRMAFRVRGVRTLNTLS
AHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALTRPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSR
GSIHAVSPDPARAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYAPEEILPGAQYLSEAELIAAAGAVGTTIFHP
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGAPERGAAASVEAALP
T

Sequences:

>Translated_561_residues
MATERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNG
RALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWAHATGDAGWSWDSVLPIFRRSEHHHAGATDVHGAGGMWRVEKQRLRW
EILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
YLGGGAPFLARARIEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVVADLRGVGENLQDHLQLRMAFRVRGVRTLNTLS
AHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALTRPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSR
GSIHAVSPDPARAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYAPEEILPGAQYLSEAELIAAAGAVGTTIFHP
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGAPERGAAASVEAALP
T
>Mature_560_residues
ATERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNGR
ALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWAHATGDAGWSWDSVLPIFRRSEHHHAGATDVHGAGGMWRVEKQRLRWE
ILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVEY
LGGGAPFLARARIEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVVADLRGVGENLQDHLQLRMAFRVRGVRTLNTLSA
HWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALTRPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRG
SIHAVSPDPARAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYAPEEILPGAQYLSEAELIAAAGAVGTTIFHPV
GTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGAPERGAAASVEAALPT

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=42.1933085501859, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1786503, Length=559, Percent_Identity=38.819320214669, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17532301, Length=565, Percent_Identity=34.6902654867257, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI45549471, Length=571, Percent_Identity=36.6024518388792, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI45551458, Length=571, Percent_Identity=36.6024518388792, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24642055, Length=576, Percent_Identity=37.5, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24642042, Length=581, Percent_Identity=35.4561101549053, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24642059, Length=568, Percent_Identity=35.7394366197183, Blast_Score=309, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24642048, Length=577, Percent_Identity=34.4887348353553, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24650267, Length=566, Percent_Identity=34.2756183745583, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI18859995, Length=572, Percent_Identity=35.1398601398601, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI17137792, Length=558, Percent_Identity=33.8709677419355, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=35.0262697022767, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=32.8097731239092, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=32.404181184669, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859993, Length=574, Percent_Identity=30.4878048780488, Blast_Score=252, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24642035, Length=303, Percent_Identity=40.2640264026403, Blast_Score=214, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=28.5223367697594, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24642057, Length=602, Percent_Identity=28.9036544850498, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 60642; Mature: 60511

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGKDDYHWIHIPVGYLYC
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEEEEE
IGNPRTDWLYKTEPEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWAHATGDA
CCCCCCCCEEECCCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCCCCHHCCCCCC
GWSWDSVLPIFRRSEHHHAGATDVHGAGGMWRVEKQRLRWEILEAFSQAAQQTGIPATDD
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNRGDNTGVGYFEVNQKRGVRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
CCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCE
YLGGGAPFLARARIEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVVADLRGVGENLQD
EECCCCCHHHHEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHH
HLQLRMAFRVRGVRTLNTLSAHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPA
HHHHHHHHHHCCCHHHHHHHHCCCEEEEECCEEHHHCCCCCCCCHHHHHHHHCCCCCCCC
LTRPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRGSIHAVSPDPARAPAIAPNY
CCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEEEECCCCCCCCCCCCCC
LSTDHDRHVAANALRLTRRIAAAPALARYAPEEILPGAQYLSEAELIAAAGAVGTTIFHP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHCC
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIR
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHH
ADRRGAPERGAAASVEAALPT
HHHCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
ATERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGKDDYHWIHIPVGYLYC
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEEEEE
IGNPRTDWLYKTEPEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWAHATGDA
CCCCCCCCEEECCCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCCCCHHCCCCCC
GWSWDSVLPIFRRSEHHHAGATDVHGAGGMWRVEKQRLRWEILEAFSQAAQQTGIPATDD
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNRGDNTGVGYFEVNQKRGVRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
CCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCE
YLGGGAPFLARARIEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVVADLRGVGENLQD
EECCCCCHHHHEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHH
HLQLRMAFRVRGVRTLNTLSAHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPA
HHHHHHHHHHCCCHHHHHHHHCCCEEEEECCEEHHHCCCCCCCCHHHHHHHHCCCCCCCC
LTRPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRGSIHAVSPDPARAPAIAPNY
CCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEEEECCCCCCCCCCCCCC
LSTDHDRHVAANALRLTRRIAAAPALARYAPEEILPGAQYLSEAELIAAAGAVGTTIFHP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHCC
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIR
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHH
ADRRGAPERGAAASVEAALPT
HHHCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]