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The map label for this gene is wcaJ [H]

Identifier: 83719142

GI number: 83719142

Start: 607836

End: 609221

Strand: Direct

Name: wcaJ [H]

Synonym: BTH_I0535

Alternate gene names: 83719142

Gene position: 607836-609221 (Clockwise)

Preceding gene: 83721346

Following gene: 83719778

Centisome position: 15.96

GC content: 66.45

Gene sequence:

>1386_bases
ATGTTGGGACTATTGAACAAATTGCTCGATGTGGTGCTGGTCGCGGCGGGCGCGCTGCTCGCGCAGATGCTGTACGACGG
CGGCATCCTCGACGTGTCCGACGAGCAGCGCACGGTCATCGCGCTGCTGTGCGCGCTGACGCTGCTGGTCTTTCCCGCCA
TCGGCGTTTATGACGGCGCGCGCGAGCAGATGTCGTATCGCACGCTGTCGCGCGTGCTGCTCGGCTGGCTCGGCGTCGCG
GCGATCGCCGCGGCTTTCATGCTGCTGCTGCAGGGCGACGCCGACGTGTCGCTCGCGTGGCTCGGCCGGACGATGCTGAT
GTCGGGCGCGGTGTTGCTGCTCGGCAAGGCGTCGATCCACGGCGTGCTCAACGGCCTGCGCCGCACGGGCGCGAAGCCGC
GCGCGGTCGCGATCGTCGGCTCCGAAGTCTACGGCCGTGCGGTGCTCGAGCAACTGCGCGCGTCGCCGTCGCACGGCTAC
GAGGTCGCATGCGTGTTCGACGACAGCGTGAGCTCGGCCGATGCCGCCGAGCGGCGCGTGGGCGGCGTGCCCGTCGTCGC
CGATCTGCGCGAGCTGAAGCGCCGCGTGCGCGCGCGCGCGATCAACGAGATCTGGCTTGCGCTGCCGCTGTCGCACGAGC
GTCAGATCCAGCGGATCGTGCGCGAGTTTCGCCACGATTTCGTGAACCTGCGCTTCCTGCCCGACGTGCGCGGGATCACG
TTCTTCAACCGGTCGGTCACGCAGGTCGTCGGCATGCCCGCGATCAATCTCGCGACGAGCCCGCTGTCGATCCCGCAGCT
CTGGCCGAAATTCATCTTCGATCGCCTGTTCGCGCTCGCGGTGCTGATTCCGTTGTCGCCGATTCTCGCGGCGCTCGCGC
TCGCGGTGAAGTTCTCGTCGCCGGGGCCCGTGCTGTTCCGGCAAAAGCGCAAGGGCGTCGACGGCCGCGAGTTCGAGATT
CTGAAATTCCGCACGATGCGCGTGCATGCCGAGGAAGCGGGCGTCGTGCGCCAGGCGTCGCGCAACGATTCGCGGATCAC
GAAGGTCGGCGCGTTCCTGCGCCGCACGTCGCTCGACGAGCTGCCGCAGTTCTTCAACGTGCTGTTCGGCCAGATGTCCG
TCGTCGGTCCGCGTCCGCACGCGATCGAGCACGACGACATCTACAAGGAACTCGTCGACGGCTACATGTACCGCTATCGC
GTGCGTCCCGGCATCACCGGCTGGGCGCAGGTGAACGGTTATCGAGGCGAGACGCGCAAGGTCGAGAAGATGGCCGCGCG
CGTGAAGTTCGATCTTTTCTACATGCAGAACTGGACCTTCTGGTTCGACATCAAGATCATCCTGATCACGCTCGTCAAGG
GATTCGTCGGCCGCAACGCGTTCTGA

Upstream 100 bases:

>100_bases
CGATTCCGGCGGAAGGCCGGCCGCTGCGCGTCGCCGGCTATCACGGCGCGGTGCGCTCGGCCGCTTGAGACGACGGAACA
CGGAACGCGGAGATTCGGTA

Downstream 100 bases:

>100_bases
GCCGGCGCGCGTTCGCTGCGAACGCGCCGCAATCCGCTTCGACGACCACGCTACCAAGGAGAAACGACAAGATGTTCAAG
CCCCTAGCATGGGCGAGCGC

Product: polysaccharide biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLGLLNKLLDVVLVAAGALLAQMLYDGGILDVSDEQRTVIALLCALTLLVFPAIGVYDGAREQMSYRTLSRVLLGWLGVA
AIAAAFMLLLQGDADVSLAWLGRTMLMSGAVLLLGKASIHGVLNGLRRTGAKPRAVAIVGSEVYGRAVLEQLRASPSHGY
EVACVFDDSVSSADAAERRVGGVPVVADLRELKRRVRARAINEIWLALPLSHERQIQRIVREFRHDFVNLRFLPDVRGIT
FFNRSVTQVVGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKFSSPGPVLFRQKRKGVDGREFEI
LKFRTMRVHAEEAGVVRQASRNDSRITKVGAFLRRTSLDELPQFFNVLFGQMSVVGPRPHAIEHDDIYKELVDGYMYRYR
VRPGITGWAQVNGYRGETRKVEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF

Sequences:

>Translated_461_residues
MLGLLNKLLDVVLVAAGALLAQMLYDGGILDVSDEQRTVIALLCALTLLVFPAIGVYDGAREQMSYRTLSRVLLGWLGVA
AIAAAFMLLLQGDADVSLAWLGRTMLMSGAVLLLGKASIHGVLNGLRRTGAKPRAVAIVGSEVYGRAVLEQLRASPSHGY
EVACVFDDSVSSADAAERRVGGVPVVADLRELKRRVRARAINEIWLALPLSHERQIQRIVREFRHDFVNLRFLPDVRGIT
FFNRSVTQVVGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKFSSPGPVLFRQKRKGVDGREFEI
LKFRTMRVHAEEAGVVRQASRNDSRITKVGAFLRRTSLDELPQFFNVLFGQMSVVGPRPHAIEHDDIYKELVDGYMYRYR
VRPGITGWAQVNGYRGETRKVEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF
>Mature_461_residues
MLGLLNKLLDVVLVAAGALLAQMLYDGGILDVSDEQRTVIALLCALTLLVFPAIGVYDGAREQMSYRTLSRVLLGWLGVA
AIAAAFMLLLQGDADVSLAWLGRTMLMSGAVLLLGKASIHGVLNGLRRTGAKPRAVAIVGSEVYGRAVLEQLRASPSHGY
EVACVFDDSVSSADAAERRVGGVPVVADLRELKRRVRARAINEIWLALPLSHERQIQRIVREFRHDFVNLRFLPDVRGIT
FFNRSVTQVVGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKFSSPGPVLFRQKRKGVDGREFEI
LKFRTMRVHAEEAGVVRQASRNDSRITKVGAFLRRTSLDELPQFFNVLFGQMSVVGPRPHAIEHDDIYKELVDGYMYRYR
VRPGITGWAQVNGYRGETRKVEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=468, Percent_Identity=37.8205128205128, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51392; Mature: 51392

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLLNKLLDVVLVAAGALLAQMLYDGGILDVSDEQRTVIALLCALTLLVFPAIGVYDGA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCH
REQMSYRTLSRVLLGWLGVAAIAAAFMLLLQGDADVSLAWLGRTMLMSGAVLLLGKASIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCHHHEEEHHHHH
GVLNGLRRTGAKPRAVAIVGSEVYGRAVLEQLRASPSHGYEVACVFDDSVSSADAAERRV
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHC
GGVPVVADLRELKRRVRARAINEIWLALPLSHERQIQRIVREFRHDFVNLRFLPDVRGIT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCC
FFNRSVTQVVGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKFSS
HHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGPVLFRQKRKGVDGREFEILKFRTMRVHAEEAGVVRQASRNDSRITKVGAFLRRTSLDE
CCCHHHHHHHCCCCCCCEEEEEEEEHEEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHH
LPQFFNVLFGQMSVVGPRPHAIEHDDIYKELVDGYMYRYRVRPGITGWAQVNGYRGETRK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHEECCCCCHHHH
VEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF
HHHHHHHHHHEEEEEECCEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLGLLNKLLDVVLVAAGALLAQMLYDGGILDVSDEQRTVIALLCALTLLVFPAIGVYDGA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCH
REQMSYRTLSRVLLGWLGVAAIAAAFMLLLQGDADVSLAWLGRTMLMSGAVLLLGKASIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCHHHEEEHHHHH
GVLNGLRRTGAKPRAVAIVGSEVYGRAVLEQLRASPSHGYEVACVFDDSVSSADAAERRV
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHC
GGVPVVADLRELKRRVRARAINEIWLALPLSHERQIQRIVREFRHDFVNLRFLPDVRGIT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCC
FFNRSVTQVVGMPAINLATSPLSIPQLWPKFIFDRLFALAVLIPLSPILAALALAVKFSS
HHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGPVLFRQKRKGVDGREFEILKFRTMRVHAEEAGVVRQASRNDSRITKVGAFLRRTSLDE
CCCHHHHHHHCCCCCCCEEEEEEEEHEEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHH
LPQFFNVLFGQMSVVGPRPHAIEHDDIYKELVDGYMYRYRVRPGITGWAQVNGYRGETRK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHEECCCCCHHHH
VEKMAARVKFDLFYMQNWTFWFDIKIILITLVKGFVGRNAF
HHHHHHHHHHEEEEEECCEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]