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The map label for this gene is gspE [C]

Identifier: 83719018

GI number: 83719018

Start: 2810229

End: 2811776

Strand: Reverse

Name: gspE [C]

Synonym: BTH_I2456

Alternate gene names: 83719018

Gene position: 2811776-2810229 (Counterclockwise)

Preceding gene: 83720991

Following gene: 83719955

Centisome position: 73.82

GC content: 70.16

Gene sequence:

>1548_bases
ATGTTCGGCCGGCGTCCGGCGTCTTCGTTCGCGCCCGGCTCAGACGGCGCCGCGCGCGAGTTCGCGGGCGACGCCGCGTC
AGCGGGCGCGGCGCCCGCCGTCGGCCATGCATCGGCGCCAGCCGCGGGCGGTGCGGCGTGCGCGGGCGATCCGGCCGGCG
CTTCGCGCGCGAACCCGCCCGCCGGCGTCTTGCGGGAAAGCGGCGCACCCGACGGCCATGCGCATGCGCCACGCGACGAT
CGCGACGGCGCGGACGACCCCGGTCACGCGCAAGACCACCACGAGGCGCTGATCCGCTCGGAGACGTTCAAGACGATCCG
CACGGTCGTCTTTTCGTCGATGAACATGTCGGCCGCGCTGATGATGTCGCGCGCCGAGGTGCGCGAAGGCATCGAGCAGA
CGGCCGCCGACGTGATCGCGCGCGAGCGCCTGACGGTGACGCTCGCCGAGCAGGCGCTCATCGTCGAGGAGATCCTCAAC
GACATGTTCGGCGTCGGCCCGATCGAGCCGCTCCTCGCGGACGAGCGCGTGACCGACATCCTCGTCAACGGCCCCGACCA
GGTGTACGTCGAGCGCGCGGGCAGGCTCGAGCTCACGCCGCTCAAGTTCCGCGACAACGCGCATGTGATCAACGTCGCGC
AGCGGATCGCGGCGGCCGTCGGGCGGCGCGTCGACGAGAGCAGCCCGATGGTCGACGCGCGGCTCGCGGACGGCAGCCGC
GTGAACGTCGTGCTGCCGCCGATCGCGATCCGCGGCGCGTCGATCTCGATTCGCAAGTTCGCGAAACGCAACATCACGCT
CGCGCGAATGGCGCAGCAGGGCAACCTGTCGCAGGCGATGGTCGAGGTGCTGAAGATCGCGAGCGCGTGCCGTCTGAACA
TCGTGATCTCGGGCGGCACGGGTTCCGGCAAGACGACGCTCCTGAACGCGCTGTCGCACTTCATCGATTCGCACGAGCGC
ATCGTGACGATCGAGGATGCGGCGGAGCTGCAATTGCAGCAGCCGCACGTGGTGAGCCTCGAGACCCGTCCGGAGAACAG
CGAGGGGCTGGGCGGCGTATCGCAGCGCGATCTCGTGCGCAACGCGCTGCGCATGCGCCCCGACCGGATCATCCTCGGCG
AGACGCGCGGCCCGGAGGCGTTCGACGTGCTGCAGGCGATGAACACGGGCCACGACGGCTCGATGACGACGATCCACGCG
AACACGCCGCGCGACGCGATCACGCGCCTCGAGAGCATGGTGATGATGGCCAACGGCAATCTGCCGCTCGTGTCGATTCG
CCGGCAGATCGCGAGCGCGGTGCACATGATCCTGCAGATCGAGCGGATGCGCGACGGCGTGCGGCGTGTCACGCGCGTGA
CCGAGATCGCCGGCATGGAAGGCGACGTCGTGATCACGCAGGATCTGTTCGCGTTCCGCTACGACGCGAGCGCGTTCCAG
GAGCAGGTGCACGGGATGTTCGAATCGTCGTCGCTGCGCCCGGCGTTCGCGCAGCGCGCCGCGTATTACGGCCTCGAGGG
CGCGCTGCTCGGCGCGCTGCAGCCGTGA

Upstream 100 bases:

>100_bases
GCATCGCCACGCTCGCGCAAGGGCTGACGGGGGCCGACGCGGCGAGCGCGTCGCGCCGGCCTTGGTACGCGCGGCTCACC
GGCGCGCGGGGAGCGCGGTG

Downstream 100 bases:

>100_bases
GCGCCGCGGACGTCGTCGCCGTCGGCGCGTTCTTCGCGATCGTCGTCGCGGGCTTCATCGCGCGCGCGTTGCGCGACCTC
GCGCGGCGGCGGCCCGCCGC

Product: component of type IV pilus

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MFGRRPASSFAPGSDGAAREFAGDAASAGAAPAVGHASAPAAGGAACAGDPAGASRANPPAGVLRESGAPDGHAHAPRDD
RDGADDPGHAQDHHEALIRSETFKTIRTVVFSSMNMSAALMMSRAEVREGIEQTAADVIARERLTVTLAEQALIVEEILN
DMFGVGPIEPLLADERVTDILVNGPDQVYVERAGRLELTPLKFRDNAHVINVAQRIAAAVGRRVDESSPMVDARLADGSR
VNVVLPPIAIRGASISIRKFAKRNITLARMAQQGNLSQAMVEVLKIASACRLNIVISGGTGSGKTTLLNALSHFIDSHER
IVTIEDAAELQLQQPHVVSLETRPENSEGLGGVSQRDLVRNALRMRPDRIILGETRGPEAFDVLQAMNTGHDGSMTTIHA
NTPRDAITRLESMVMMANGNLPLVSIRRQIASAVHMILQIERMRDGVRRVTRVTEIAGMEGDVVITQDLFAFRYDASAFQ
EQVHGMFESSSLRPAFAQRAAYYGLEGALLGALQP

Sequences:

>Translated_515_residues
MFGRRPASSFAPGSDGAAREFAGDAASAGAAPAVGHASAPAAGGAACAGDPAGASRANPPAGVLRESGAPDGHAHAPRDD
RDGADDPGHAQDHHEALIRSETFKTIRTVVFSSMNMSAALMMSRAEVREGIEQTAADVIARERLTVTLAEQALIVEEILN
DMFGVGPIEPLLADERVTDILVNGPDQVYVERAGRLELTPLKFRDNAHVINVAQRIAAAVGRRVDESSPMVDARLADGSR
VNVVLPPIAIRGASISIRKFAKRNITLARMAQQGNLSQAMVEVLKIASACRLNIVISGGTGSGKTTLLNALSHFIDSHER
IVTIEDAAELQLQQPHVVSLETRPENSEGLGGVSQRDLVRNALRMRPDRIILGETRGPEAFDVLQAMNTGHDGSMTTIHA
NTPRDAITRLESMVMMANGNLPLVSIRRQIASAVHMILQIERMRDGVRRVTRVTEIAGMEGDVVITQDLFAFRYDASAFQ
EQVHGMFESSSLRPAFAQRAAYYGLEGALLGALQP
>Mature_515_residues
MFGRRPASSFAPGSDGAAREFAGDAASAGAAPAVGHASAPAAGGAACAGDPAGASRANPPAGVLRESGAPDGHAHAPRDD
RDGADDPGHAQDHHEALIRSETFKTIRTVVFSSMNMSAALMMSRAEVREGIEQTAADVIARERLTVTLAEQALIVEEILN
DMFGVGPIEPLLADERVTDILVNGPDQVYVERAGRLELTPLKFRDNAHVINVAQRIAAAVGRRVDESSPMVDARLADGSR
VNVVLPPIAIRGASISIRKFAKRNITLARMAQQGNLSQAMVEVLKIASACRLNIVISGGTGSGKTTLLNALSHFIDSHER
IVTIEDAAELQLQQPHVVSLETRPENSEGLGGVSQRDLVRNALRMRPDRIILGETRGPEAFDVLQAMNTGHDGSMTTIHA
NTPRDAITRLESMVMMANGNLPLVSIRRQIASAVHMILQIERMRDGVRRVTRVTEIAGMEGDVVITQDLFAFRYDASAFQ
EQVHGMFESSSLRPAFAQRAAYYGLEGALLGALQP

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55022; Mature: 55022

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRRPASSFAPGSDGAAREFAGDAASAGAAPAVGHASAPAAGGAACAGDPAGASRANPP
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGVLRESGAPDGHAHAPRDDRDGADDPGHAQDHHEALIRSETFKTIRTVVFSSMNMSAAL
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MMSRAEVREGIEQTAADVIARERLTVTLAEQALIVEEILNDMFGVGPIEPLLADERVTDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHEE
LVNGPDQVYVERAGRLELTPLKFRDNAHVINVAQRIAAAVGRRVDESSPMVDARLADGSR
EECCCCEEEHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCE
VNVVLPPIAIRGASISIRKFAKRNITLARMAQQGNLSQAMVEVLKIASACRLNIVISGGT
EEEEECCEEECCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEEECCC
GSGKTTLLNALSHFIDSHERIVTIEDAAELQLQQPHVVSLETRPENSEGLGGVSQRDLVR
CCCHHHHHHHHHHHHCCCCEEEEEECHHHHEECCCCEEEEECCCCCCCCCCCCHHHHHHH
NALRMRPDRIILGETRGPEAFDVLQAMNTGHDGSMTTIHANTPRDAITRLESMVMMANGN
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEECCC
LPLVSIRRQIASAVHMILQIERMRDGVRRVTRVTEIAGMEGDVVITQDLFAFRYDASAFQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHH
EQVHGMFESSSLRPAFAQRAAYYGLEGALLGALQP
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MFGRRPASSFAPGSDGAAREFAGDAASAGAAPAVGHASAPAAGGAACAGDPAGASRANPP
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGVLRESGAPDGHAHAPRDDRDGADDPGHAQDHHEALIRSETFKTIRTVVFSSMNMSAAL
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MMSRAEVREGIEQTAADVIARERLTVTLAEQALIVEEILNDMFGVGPIEPLLADERVTDI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHEE
LVNGPDQVYVERAGRLELTPLKFRDNAHVINVAQRIAAAVGRRVDESSPMVDARLADGSR
EECCCCEEEHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCE
VNVVLPPIAIRGASISIRKFAKRNITLARMAQQGNLSQAMVEVLKIASACRLNIVISGGT
EEEEECCEEECCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEEECCC
GSGKTTLLNALSHFIDSHERIVTIEDAAELQLQQPHVVSLETRPENSEGLGGVSQRDLVR
CCCHHHHHHHHHHHHCCCCEEEEEECHHHHEECCCCEEEEECCCCCCCCCCCCHHHHHHH
NALRMRPDRIILGETRGPEAFDVLQAMNTGHDGSMTTIHANTPRDAITRLESMVMMANGN
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEECCC
LPLVSIRRQIASAVHMILQIERMRDGVRRVTRVTEIAGMEGDVVITQDLFAFRYDASAFQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHH
EQVHGMFESSSLRPAFAQRAAYYGLEGALLGALQP
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]