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The map label for this gene is ygfZ [C]

Identifier: 83718935

GI number: 83718935

Start: 2430613

End: 2431659

Strand: Reverse

Name: ygfZ [C]

Synonym: BTH_I2152

Alternate gene names: 83718935

Gene position: 2431659-2430613 (Counterclockwise)

Preceding gene: 83720847

Following gene: 83721265

Centisome position: 63.84

GC content: 73.16

Gene sequence:

>1047_bases
ATGAGCACACCGATCGCCTCTCCGGCCGCGCAGGCCGCCGCCCCCACCGCGCTGCCGACGCTGCCCCGTCCCGATGCCGA
CGATTTCGCCGCCGTGCTCGAGCGCGGCGCGTTCGCGGTGCTCGAGCAATTCGGCATCGTCGACGTGACGGGCGCCGACG
CCGCAACGTTCCTGCACGGCCAACTGACGAACGACATCGAGCATCTCGACGCCGCGAGCGCGCGCCTCGCCGGCTACTGC
TCGCCGAAGGGGCGCCTGCTCGCGTCGTTCCTCGCGTGGCGCGCCGGCCATGACGTGCGCCTGCTCGTATCGAAGGACGT
CCAGCCCGCGGTGCAAAAGCGCCTGTCGATGTTCGTGCTGCGCGCGAAGGCGAAGCTCGCCGACGCGGGCGGCACGCACG
TCGCGGTCGGCTTCGCGGGCGACGTGCGCGCCGCGCTGTCGGGCATCTTCGACGCGCTGCCGGACGGCATCCACACGAAG
GTCGACGCGCCCGCCGGCGCGCTCGTGCGGCTGCCCGACGCGGCCGGCCGCGCGCGCTACCTGTGGATCGCCGCGCGCGC
CGAGCTCGACGCGCGCCTGCCCGCGCTCGAAGCGGCGCTGCCGCGCGTGTCGGCCGCCGTCTGGGATTGGCTTGACGTGC
GCGCGGGCGAGCCGCGCGTCACGCTGCCCGCCGTCGAGCAATTCGTCCCGCAGATGGTCAACTTCGACGTGATCGGCGGC
GTCAACTTCCGCAAGGGCTGCTATCCGGGCCAGGAAGTCGTCGCGCGCAGCCAGTACCGGGGCACGATCAAGCGCCGCAC
CGCGCTCGCGCACGTCGCGGTCGGCACCGACGCCGCGCATGCGGGCGTCGAGCTCTATCATTCGGACGATCCCGGTCAGC
CGTGCGGGATGATCGTGAACGCGGCGGCGGCGCCCGAAGGCGGCGTCGACGCGCTCGTCGAGATCAAGCTCGCCGCGCTC
GAAAGCGGATCGGTCCACCTGGCGTCCGCGGGCGGACCGGCGCTCACGTTCCTGCCGCTTCCCTACGCGTTGCCCGTCGA
AGCCTGA

Upstream 100 bases:

>100_bases
CGCACTTCTTCAGTAGGGACATGCGAAACGTGAATCAGGCAAGACCCATATAATACTTGCTCGCCTCTTCCAAAGTCAGG
ATTGACTGTTCCCTCATTCC

Downstream 100 bases:

>100_bases
CGGCGCGTGCGCCGCCGGCCCCGCACGCACCGCGGCGCGCCGTCCGGCCTCACCCAGCGAGGATATCGCCCGATGTGCCT
GATCGTATTCGATTGGCAGC

Product: glycine cleavage T-protein superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSTPIASPAAQAAAPTALPTLPRPDADDFAAVLERGAFAVLEQFGIVDVTGADAATFLHGQLTNDIEHLDAASARLAGYC
SPKGRLLASFLAWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLADAGGTHVAVGFAGDVRAALSGIFDALPDGIHTK
VDAPAGALVRLPDAAGRARYLWIAARAELDARLPALEAALPRVSAAVWDWLDVRAGEPRVTLPAVEQFVPQMVNFDVIGG
VNFRKGCYPGQEVVARSQYRGTIKRRTALAHVAVGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAAL
ESGSVHLASAGGPALTFLPLPYALPVEA

Sequences:

>Translated_348_residues
MSTPIASPAAQAAAPTALPTLPRPDADDFAAVLERGAFAVLEQFGIVDVTGADAATFLHGQLTNDIEHLDAASARLAGYC
SPKGRLLASFLAWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLADAGGTHVAVGFAGDVRAALSGIFDALPDGIHTK
VDAPAGALVRLPDAAGRARYLWIAARAELDARLPALEAALPRVSAAVWDWLDVRAGEPRVTLPAVEQFVPQMVNFDVIGG
VNFRKGCYPGQEVVARSQYRGTIKRRTALAHVAVGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAAL
ESGSVHLASAGGPALTFLPLPYALPVEA
>Mature_347_residues
STPIASPAAQAAAPTALPTLPRPDADDFAAVLERGAFAVLEQFGIVDVTGADAATFLHGQLTNDIEHLDAASARLAGYCS
PKGRLLASFLAWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLADAGGTHVAVGFAGDVRAALSGIFDALPDGIHTKV
DAPAGALVRLPDAAGRARYLWIAARAELDARLPALEAALPRVSAAVWDWLDVRAGEPRVTLPAVEQFVPQMVNFDVIGGV
NFRKGCYPGQEVVARSQYRGTIKRRTALAHVAVGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAALE
SGSVHLASAGGPALTFLPLPYALPVEA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=307, Percent_Identity=27.6872964169381, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 36229; Mature: 36098

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPIASPAAQAAAPTALPTLPRPDADDFAAVLERGAFAVLEQFGIVDVTGADAATFLHG
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCEEECCCCHHHHHHH
QLTNDIEHLDAASARLAGYCSPKGRLLASFLAWRAGHDVRLLVSKDVQPAVQKRLSMFVL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
RAKAKLADAGGTHVAVGFAGDVRAALSGIFDALPDGIHTKVDAPAGALVRLPDAAGRARY
HHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEECCCCCCCEEECCCCCCCEEE
LWIAARAELDARLPALEAALPRVSAAVWDWLDVRAGEPRVTLPAVEQFVPQMVNFDVIGG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCEEEECC
VNFRKGCYPGQEVVARSQYRGTIKRRTALAHVAVGTDAAHAGVELYHSDDPGQPCGMIVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCEEEEECCCCCCCCCCEEE
AAAAPEGGVDALVEIKLAALESGSVHLASAGGPALTFLPLPYALPVEA
CCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
STPIASPAAQAAAPTALPTLPRPDADDFAAVLERGAFAVLEQFGIVDVTGADAATFLHG
CCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCEEECCCCHHHHHHH
QLTNDIEHLDAASARLAGYCSPKGRLLASFLAWRAGHDVRLLVSKDVQPAVQKRLSMFVL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
RAKAKLADAGGTHVAVGFAGDVRAALSGIFDALPDGIHTKVDAPAGALVRLPDAAGRARY
HHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEECCCCCCCEEECCCCCCCEEE
LWIAARAELDARLPALEAALPRVSAAVWDWLDVRAGEPRVTLPAVEQFVPQMVNFDVIGG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCEEEECC
VNFRKGCYPGQEVVARSQYRGTIKRRTALAHVAVGTDAAHAGVELYHSDDPGQPCGMIVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCEEEEECCCCCCCCCCEEE
AAAAPEGGVDALVEIKLAALESGSVHLASAGGPALTFLPLPYALPVEA
CCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA