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The map label for this gene is alkJ [H]

Identifier: 83718932

GI number: 83718932

Start: 57884

End: 59554

Strand: Reverse

Name: alkJ [H]

Synonym: BTH_I0054

Alternate gene names: 83718932

Gene position: 59554-57884 (Counterclockwise)

Preceding gene: 83719542

Following gene: 83719428

Centisome position: 1.56

GC content: 71.15

Gene sequence:

>1671_bases
ATGTCCGCGGCAACCCCCAAAGGCTCGACGGAATTCGACTACATCGTGATCGGCGGCGGCTCGGCCGGCTGCGTCGCCAC
GCACCGGCTCGTCAATGCCGGGCATCGCGTGCTGCTGCTCGAAGCCGGCCCGCCCGACAATTCGTTCTTCGTCCACACGC
CCGCGACGTTCGTGCGCGTGATCGGCACGAAGCGGACCTGGGTCTACGAGACGGAGCCGCAGGCGCACGCGGCCGGCCGG
CGCATGTACGTGCCGCAGGGCCGCACGCTCGGCGGCGGCAGTTCGGTGAACGCGATGGTCTACATCCGCGGCACGCCCGC
CGATTACGACGGCTGGCGCGACGCCGGCTGCGACGGCTGGGGCTGGGACGACGTGCTGCCGTTTTTCCGGCGCGCCGAGC
GCAACCACCGGCTCGCGGGGCCGCTGCACGGCGCCGACGGGCCGCTGCACGTGAGCGACGCGCGCTTTCGTCATCCGCTG
AGCGACGCGTTCGTGCAGGGCGCGCAGGAGTTCGGGCTGCCGTACAACGACGACTTCAACGGCGCGTCGCAGGCGGGCGT
CGGCTTCTATCAGACGACGACGTTCGAAGGCCGGCGCGGCAGCACCGCCGCGACGTATCTCGCCGCCGTCAAGCGCGATC
CGCTGCTGACGATCGAGACCGACGCGTTCGTCACGCGCATCGTGTTCGAGAACGGCGCGGCGGTCGGCGTGCGCTACCGT
GCGCGCGGCGGCGAGGAGCGGCTCGTGCGCGCACGCGCGGAGATCGTGCTTTGCGCGGGCGCGCTCGCGAGCCCGAAGCT
CCTGATGCTGTCGGGCATCGGCCCGGCGGATCAGCTCCGGCAGCACGGCATCGCGGTCGTGCACGATTCGCCCGAGGTCG
GACTCAACTTCCAGGACCATCTCGAAGTGTCGCTGTATGGCCGCGCGCGCGCGCCGATCAGCCTCGCGGGCCAGGACCGC
GGGCTCAACGCGCTGCGCCACGGCATCCAGTACACGCTGTTCCACACGGGGCTCCTCACGTCGAACGTCGTCGAGAGCGG
CGGCTTCGTCGATACCGCGAACGGCGGCCGCCCCGACGTGCAGTTCCACGTGCTGCCCGTGCTCGTCGGCGACGTCGGCC
GCGAGCCGCTCGAAGGGCACGGGATCTCGATCAACCCGTGCTTCCTGCGGCCGAAGTCGCGCGGCACGGTGCGCCTGCGC
AACGCCGATCCGCTCGCGCCGATTCTGTTCGACGGCAATTTCCTCAGTCATCCGGACGATTTCGCGACGCTCGTGCGCGG
GCTTTCGCTCGCGCGCGAGATCATGCGCATGCCGTCGATGTCGAAGGCGATCGCGGGCGAGATGCTGCCGACCGACGGCG
GCCGCGTCGATCTCGACGCATACGTGCGCTCGCACGCGAAGACCGTCTATCACCCGTCGGGCACGTGCCGGATGGGCGGC
GATCCGGGCTCGGTCGTCGATTCGCAGCTGCGCGTGCGCGGCGTCGGCGGGCTGAGGATCTGCGATGCGTCGGTGATGCC
GTCGCTCGTGTCCGGCAACACGAATGCGCCGACGATCATGATCGCCGAGCGCTGCGCCGAATTCATGCTGTCGCCCGCGC
TCGCGCCGAACGCGCGCGCCGGCGCGAGCGCGCCGCGGGCCGCACCGCTGGACGCGGGCCATGCGCGCTGA

Upstream 100 bases:

>100_bases
CGCAGTTTGAATTGATCGATAGCGCACACCGCGCGCACGCCGGCCGATTTCGCCGATAGTGGCAACCTGTCCATCCATCC
GACCCAGCGAGAAGCGACAT

Downstream 100 bases:

>100_bases
GTGTCCCGCGTTCGTCGTTTCGTCGCGCCGTCGGGCAAATCGTCACGATCGCGCTTTCACGTTCATTCGAAGGAGACGTC
AGTCATGCACACGTTCAACG

Product: GMC oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSAATPKGSTEFDYIVIGGGSAGCVATHRLVNAGHRVLLLEAGPPDNSFFVHTPATFVRVIGTKRTWVYETEPQAHAAGR
RMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGWGWDDVLPFFRRAERNHRLAGPLHGADGPLHVSDARFRHPL
SDAFVQGAQEFGLPYNDDFNGASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTIETDAFVTRIVFENGAAVGVRYR
ARGGEERLVRARAEIVLCAGALASPKLLMLSGIGPADQLRQHGIAVVHDSPEVGLNFQDHLEVSLYGRARAPISLAGQDR
GLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDVQFHVLPVLVGDVGREPLEGHGISINPCFLRPKSRGTVRLR
NADPLAPILFDGNFLSHPDDFATLVRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
DPGSVVDSQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPNARAGASAPRAAPLDAGHAR

Sequences:

>Translated_556_residues
MSAATPKGSTEFDYIVIGGGSAGCVATHRLVNAGHRVLLLEAGPPDNSFFVHTPATFVRVIGTKRTWVYETEPQAHAAGR
RMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGWGWDDVLPFFRRAERNHRLAGPLHGADGPLHVSDARFRHPL
SDAFVQGAQEFGLPYNDDFNGASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTIETDAFVTRIVFENGAAVGVRYR
ARGGEERLVRARAEIVLCAGALASPKLLMLSGIGPADQLRQHGIAVVHDSPEVGLNFQDHLEVSLYGRARAPISLAGQDR
GLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDVQFHVLPVLVGDVGREPLEGHGISINPCFLRPKSRGTVRLR
NADPLAPILFDGNFLSHPDDFATLVRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
DPGSVVDSQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPNARAGASAPRAAPLDAGHAR
>Mature_555_residues
SAATPKGSTEFDYIVIGGGSAGCVATHRLVNAGHRVLLLEAGPPDNSFFVHTPATFVRVIGTKRTWVYETEPQAHAAGRR
MYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGWGWDDVLPFFRRAERNHRLAGPLHGADGPLHVSDARFRHPLS
DAFVQGAQEFGLPYNDDFNGASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTIETDAFVTRIVFENGAAVGVRYRA
RGGEERLVRARAEIVLCAGALASPKLLMLSGIGPADQLRQHGIAVVHDSPEVGLNFQDHLEVSLYGRARAPISLAGQDRG
LNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDVQFHVLPVLVGDVGREPLEGHGISINPCFLRPKSRGTVRLRN
ADPLAPILFDGNFLSHPDDFATLVRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGGD
PGSVVDSQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPNARAGASAPRAAPLDAGHAR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=549, Percent_Identity=41.1657559198543, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1786503, Length=556, Percent_Identity=39.9280575539568, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17532301, Length=542, Percent_Identity=33.7638376383764, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24642042, Length=564, Percent_Identity=37.9432624113475, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24642055, Length=569, Percent_Identity=37.9613356766257, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI45551458, Length=564, Percent_Identity=37.2340425531915, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI45549471, Length=564, Percent_Identity=37.2340425531915, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24642048, Length=578, Percent_Identity=35.6401384083045, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24642059, Length=564, Percent_Identity=36.1702127659575, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24650267, Length=567, Percent_Identity=34.2151675485009, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI17137792, Length=554, Percent_Identity=34.2960288808664, Blast_Score=277, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24642039, Length=564, Percent_Identity=34.3971631205674, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI18859995, Length=565, Percent_Identity=33.2743362831858, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=33.4494773519164, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24642037, Length=575, Percent_Identity=34.9565217391304, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI18859993, Length=579, Percent_Identity=34.7150259067358, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642035, Length=310, Percent_Identity=40.3225806451613, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24645930, Length=579, Percent_Identity=28.4974093264249, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24642057, Length=587, Percent_Identity=28.1090289608177, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59354; Mature: 59223

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATPKGSTEFDYIVIGGGSAGCVATHRLVNAGHRVLLLEAGPPDNSFFVHTPATFVRV
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEECHHHHHHE
IGTKRTWVYETEPQAHAAGRRMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGW
ECCCCEEEEECCCCHHHCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GWDDVLPFFRRAERNHRLAGPLHGADGPLHVSDARFRHPLSDAFVQGAQEFGLPYNDDFN
CHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHCCCHHHHHHHHHHHHCCCCCCCCC
GASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTIETDAFVTRIVFENGAAVGVRYR
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECHHEEEEEEECCCCEEEEEEE
ARGGEERLVRARAEIVLCAGALASPKLLMLSGIGPADQLRQHGIAVVHDSPEVGLNFQDH
CCCCHHHHHHHHEEEEEEECCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCHHHC
LEVSLYGRARAPISLAGQDRGLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDV
EEEEEEECCCCCEEECCCCCCHHHHHHCCEEEEEECCHHHHHHHHCCCEEECCCCCCCCE
QFHVLPVLVGDVGREPLEGHGISINPCFLRPKSRGTVRLRNADPLAPILFDGNFLSHPDD
EEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCEEEECCCCCCCCHH
FATLVRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEHHHHHHHCCCEEECCCCCEEECC
DPGSVVDSQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPNARA
CCCCHHCCCEEEECCCCEEEECHHHCCHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCC
GASAPRAAPLDAGHAR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAATPKGSTEFDYIVIGGGSAGCVATHRLVNAGHRVLLLEAGPPDNSFFVHTPATFVRV
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEECHHHHHHE
IGTKRTWVYETEPQAHAAGRRMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGW
ECCCCEEEEECCCCHHHCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GWDDVLPFFRRAERNHRLAGPLHGADGPLHVSDARFRHPLSDAFVQGAQEFGLPYNDDFN
CHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHCCCHHHHHHHHHHHHCCCCCCCCC
GASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTIETDAFVTRIVFENGAAVGVRYR
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECHHEEEEEEECCCCEEEEEEE
ARGGEERLVRARAEIVLCAGALASPKLLMLSGIGPADQLRQHGIAVVHDSPEVGLNFQDH
CCCCHHHHHHHHEEEEEEECCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCHHHC
LEVSLYGRARAPISLAGQDRGLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDV
EEEEEEECCCCCEEECCCCCCHHHHHHCCEEEEEECCHHHHHHHHCCCEEECCCCCCCCE
QFHVLPVLVGDVGREPLEGHGISINPCFLRPKSRGTVRLRNADPLAPILFDGNFLSHPDD
EEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCEEEECCCCCCCCHH
FATLVRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEHHHHHHHCCCEEECCCCCEEECC
DPGSVVDSQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPNARA
CCCCHHCCCEEEECCCCEEEECHHHCCHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCC
GASAPRAAPLDAGHAR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]