Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 83718812

GI number: 83718812

Start: 3442541

End: 3443635

Strand: Reverse

Name: thiE [H]

Synonym: BTH_I3005

Alternate gene names: 83718812

Gene position: 3443635-3442541 (Counterclockwise)

Preceding gene: 83719527

Following gene: 83721569

Centisome position: 90.4

GC content: 74.52

Gene sequence:

>1095_bases
ATGAGCGCCGCGCTGCCCGACGCGTTCTGGCCGCCCGCGGACGAACTCTCCGAGGCCGCCGAGCGGATCCGCGCGGCGCT
CGGCGTGTGGCCGCGGCCCGATGCGCGCACGCGAATCTGCCTCGCGCCGCCCGAGCATCCGCGCGCGGGCGACCTGTGGG
TCGCCGCCGCGGGCGACGCCGACGTGCACATCGCGCGGCTGACCGCGGCGGGCGCGCAGGCGATCGTCATCGATGACGTA
TCGGCGACGCTCCATACGGGCGCGGCGCGCCACGCGCTCGCTTCGCGCGCGCCGCTCGCCGACGACTGGATCGCGGCGCT
CGCCGCCTTTCTCGATTGCGGCTTCGCCGCGCCCGACGCGCTCGTGCTCGCGCTCGCGTGGCGGGACGGTGACGAGGCGC
GCGGCGACGATCCGTGGCCCGTCGATCCGGCGCGCTTTCCGCGCGTGCTCGGCGTGCCGGCCGCGCCCGAGCCCGCGTTC
CCGCCGTGTCCGCAGCGGCTCGGCCTGTATCCGGTGCTGCCGAGCGCCGAATGGGTCGAGCGCGTGCTCGACTGCGGCGT
GCGGACCGTGCAGCTGCGCGTGAAGGACGCGTCGCCCGACGCGCTGCGCGCGGAGGTCGAGCGGGCCGTCGCCGCGGGCC
GCCGTCATCCGGACGCGCGCGTGTTCATCAACGATCACTGGCGGCTCGCGCTCGACGCGGGCGCGTACGGCATCCACCTC
GGCCAGGAGGATCTCGAGACCGCCGAGCTTCGAGCGATCGCGCAGGCCGGCGTGCGGCTCGGCCTGTCGAGCCACGGCTA
TTACGAAATGCTCGTCGCGTTGCAGTTGAAGCCGAGCTATCTCGCGCTCGGCCCGGTGTTCGCGACCGCGACGAAGGCGG
TCGCCGCGCCGCCGCAGGGCCTCGCGCGGCTCGCGCGCTATGCGCGCTTCGCGGGGCCGCAGGCGCCGCTCGTCGCGATC
GGCGGAATCACTGTCGACACACTCGGCGCGGTGCTGGCGGCGGGCGTCGGCAGCGCGGCCGTCGTCAGCGCGATCACGGC
AGCGGCCGATTACCGGGAAGCGATTGTTGCAATGCAGAAAAACTTCGGACGATAA

Upstream 100 bases:

>100_bases
GCGCCGCGTATCTCGCGGGGCCGATGGATGCCCGCGAGAGCGCGCATGCGAGCACGCCCGTCGTCGGCATGCCGTTCTGG
CACCAGGACGGGAGCCACGC

Downstream 100 bases:

>100_bases
TTTGACAATCTCTGACTGGGGCGCCTGAGAAGCCTTCACGACATCATTCCGATGCAGGCCCTATAATTCGGCGTTCTGCG
TATAAGGATTGTCCGCTCCG

Product: thiamin-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSAALPDAFWPPADELSEAAERIRAALGVWPRPDARTRICLAPPEHPRAGDLWVAAAGDADVHIARLTAAGAQAIVIDDV
SATLHTGAARHALASRAPLADDWIAALAAFLDCGFAAPDALVLALAWRDGDEARGDDPWPVDPARFPRVLGVPAAPEPAF
PPCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYGIHL
GQEDLETAELRAIAQAGVRLGLSSHGYYEMLVALQLKPSYLALGPVFATATKAVAAPPQGLARLARYARFAGPQAPLVAI
GGITVDTLGAVLAAGVGSAAVVSAITAAADYREAIVAMQKNFGR

Sequences:

>Translated_364_residues
MSAALPDAFWPPADELSEAAERIRAALGVWPRPDARTRICLAPPEHPRAGDLWVAAAGDADVHIARLTAAGAQAIVIDDV
SATLHTGAARHALASRAPLADDWIAALAAFLDCGFAAPDALVLALAWRDGDEARGDDPWPVDPARFPRVLGVPAAPEPAF
PPCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYGIHL
GQEDLETAELRAIAQAGVRLGLSSHGYYEMLVALQLKPSYLALGPVFATATKAVAAPPQGLARLARYARFAGPQAPLVAI
GGITVDTLGAVLAAGVGSAAVVSAITAAADYREAIVAMQKNFGR
>Mature_363_residues
SAALPDAFWPPADELSEAAERIRAALGVWPRPDARTRICLAPPEHPRAGDLWVAAAGDADVHIARLTAAGAQAIVIDDVS
ATLHTGAARHALASRAPLADDWIAALAAFLDCGFAAPDALVLALAWRDGDEARGDDPWPVDPARFPRVLGVPAAPEPAFP
PCPQRLGLYPVLPSAEWVERVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYGIHLG
QEDLETAELRAIAQAGVRLGLSSHGYYEMLVALQLKPSYLALGPVFATATKAVAAPPQGLARLARYARFAGPQAPLVAIG
GITVDTLGAVLAAGVGSAAVVSAITAAADYREAIVAMQKNFGR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=199, Percent_Identity=56.78391959799, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38243; Mature: 38112

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALPDAFWPPADELSEAAERIRAALGVWPRPDARTRICLAPPEHPRAGDLWVAAAGDA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCC
DVHIARLTAAGAQAIVIDDVSATLHTGAARHALASRAPLADDWIAALAAFLDCGFAAPDA
CEEEEEEECCCCCEEEEECCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
LVLALAWRDGDEARGDDPWPVDPARFPRVLGVPAAPEPAFPPCPQRLGLYPVLPSAEWVE
EEEEEEECCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHHCCCEECCCCHHHHH
RVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYGIHL
HHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCEEEEC
GQEDLETAELRAIAQAGVRLGLSSHGYYEMLVALQLKPSYLALGPVFATATKAVAAPPQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHCCCHHH
LARLARYARFAGPQAPLVAIGGITVDTLGAVLAAGVGSAAVVSAITAAADYREAIVAMQK
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NFGR
HCCC
>Mature Secondary Structure 
SAALPDAFWPPADELSEAAERIRAALGVWPRPDARTRICLAPPEHPRAGDLWVAAAGDA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCC
DVHIARLTAAGAQAIVIDDVSATLHTGAARHALASRAPLADDWIAALAAFLDCGFAAPDA
CEEEEEEECCCCCEEEEECCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
LVLALAWRDGDEARGDDPWPVDPARFPRVLGVPAAPEPAFPPCPQRLGLYPVLPSAEWVE
EEEEEEECCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCHHHCCCEECCCCHHHHH
RVLDCGVRTVQLRVKDASPDALRAEVERAVAAGRRHPDARVFINDHWRLALDAGAYGIHL
HHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCEEEEC
GQEDLETAELRAIAQAGVRLGLSSHGYYEMLVALQLKPSYLALGPVFATATKAVAAPPQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEHHHHHHHHHHHHCCCHHH
LARLARYARFAGPQAPLVAIGGITVDTLGAVLAAGVGSAAVVSAITAAADYREAIVAMQK
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NFGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA