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The map label for this gene is fhlA [H]

Identifier: 83718770

GI number: 83718770

Start: 2571887

End: 2573242

Strand: Direct

Name: fhlA [H]

Synonym: BTH_I2283

Alternate gene names: 83718770

Gene position: 2571887-2573242 (Clockwise)

Preceding gene: 83720931

Following gene: 83719551

Centisome position: 67.52

GC content: 70.58

Gene sequence:

>1356_bases
ATGGATCAGCACGACGTCATCCCGATCGTTCCCGTTTCGCCGCCCGACGTGGGCGTGCTCGTGTCGTACCTCGAACAGGA
CCCGCAACCGATGATCGTGCTCGATCCGTCGTACCGAATCCTCGCCGCGAACGCCGCGTATCAGCGGCAGTTCGGGATTG
CCGGCGAGGCGCACGTCGGGCGGCGCTGCTATCAGGTCTCGCATCATTACGACGTGCCGTGCGATCAGGCGGGCGAGCAT
TGCCCGATGAAGCAGGCGACCGAATCGCGCAGCCTGAACCGCGTGCTGCACATCCATCACACGCCGCGCGGGCCCGAGCA
CGTCGACGTCGAGCTGCGGCCGATCTTCGACGCGCACGGTGCGGTGATCGCGTACGTCGAGCGGCTGACGACCGTGCGCA
GCGCGTCGGCCAAGCCGAGCGGCGAGGGGCTCGTCGGCGGCTCGGATGCGTTCAACGCGGCCCTGTCCGCGCTGCAGCGG
GTTGCACCTTCGACGCTGCCCGTGCTGCTGCTCGGCGAATCGGGCACGGGCAAGGAATTGTTCGCGCGCGCCCTGCACGA
GGCGAGCGCGCGCGCGATGGGGCCGTTCGTCGTGGTCGATTGCTCGGGGATCGCCGAGACGCTGTTCGAGAGCGAACTGT
TCGGCTACGAGAAGGGCGCGTTCACCGGTGCGACCGCGCGCAAGCCCGGCCTCGTGGAGACCGCGCAGGGCGGCACGCTG
TTTCTCGACGAGATCGGCGACGTGCCGCTGTCGATGCAGGTGAAGCTGCTGCGCCTCATCGAATCGGGGACATTCCGGCG
AGTGGGCGGCGTCGAGGTGCTGCGCGCGGATTTCCGGCTCGTCGCGGCGACTCACAAGCCGCTGAAGGCGATGATCGGCG
ATGGGCGGTTCCGGCCGGATCTGTACTACCGGATCAGCGCGTACCCGATTGCGCTGCCTGCGGTGCGCGAGCGGCCGGGC
GACATGCCGCTTCTCGTCGATTCGATCCTGCGCCGGATCGCGGCGCTCGGGCCGGCTGCCGGGCAGCGCTTCACCGTCGC
GCCCGATGCGCTCGCGCGGCTCGAGGCGTACGCGTGGCCGGGCAACATCCGCGAACTGCGCAACGTGCTCGATCGCGCAT
GCCTGCTCACGGACGACGGCGTGATCCGCGTCGAGCACCTGCCCGACGAGGTCGCGCGCGCGGGCGACGCGCGCGAGGAG
GCGGGCGCGTCGGCGAAGCTGTCGGACGACGAGCTCGCGCGGATTGCCCGCGCGTTCGTTGGCACGCGGCGCGCGCTCGC
CGGGCGAGTCGGGATGAGCGAGCGCACGCTGTACCGGCGGCTGCGCGCGCTCGGGATCGCGACGCGGGACGCTTGA

Upstream 100 bases:

>100_bases
CTCCCGGGCGACGATCGTTCAAGTTCGCTGCCGTGAATGGCAGTTTGATGGCAGAATTGGCAGCTACGCGGCCGTGGGCC
GCGCAGCCGGAGCCGAACCC

Downstream 100 bases:

>100_bases
GGGCGGACCCGGGCGGGGCGCGACGGCAAGTGCGCTACATGCGGGCGTCGACGTCCGGTGCCCCTGCGGAGCATTCGTCG
CTCGCGATGCAGTTTGATGC

Product: sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MDQHDVIPIVPVSPPDVGVLVSYLEQDPQPMIVLDPSYRILAANAAYQRQFGIAGEAHVGRRCYQVSHHYDVPCDQAGEH
CPMKQATESRSLNRVLHIHHTPRGPEHVDVELRPIFDAHGAVIAYVERLTTVRSASAKPSGEGLVGGSDAFNAALSALQR
VAPSTLPVLLLGESGTGKELFARALHEASARAMGPFVVVDCSGIAETLFESELFGYEKGAFTGATARKPGLVETAQGGTL
FLDEIGDVPLSMQVKLLRLIESGTFRRVGGVEVLRADFRLVAATHKPLKAMIGDGRFRPDLYYRISAYPIALPAVRERPG
DMPLLVDSILRRIAALGPAAGQRFTVAPDALARLEAYAWPGNIRELRNVLDRACLLTDDGVIRVEHLPDEVARAGDAREE
AGASAKLSDDELARIARAFVGTRRALAGRVGMSERTLYRRLRALGIATRDA

Sequences:

>Translated_451_residues
MDQHDVIPIVPVSPPDVGVLVSYLEQDPQPMIVLDPSYRILAANAAYQRQFGIAGEAHVGRRCYQVSHHYDVPCDQAGEH
CPMKQATESRSLNRVLHIHHTPRGPEHVDVELRPIFDAHGAVIAYVERLTTVRSASAKPSGEGLVGGSDAFNAALSALQR
VAPSTLPVLLLGESGTGKELFARALHEASARAMGPFVVVDCSGIAETLFESELFGYEKGAFTGATARKPGLVETAQGGTL
FLDEIGDVPLSMQVKLLRLIESGTFRRVGGVEVLRADFRLVAATHKPLKAMIGDGRFRPDLYYRISAYPIALPAVRERPG
DMPLLVDSILRRIAALGPAAGQRFTVAPDALARLEAYAWPGNIRELRNVLDRACLLTDDGVIRVEHLPDEVARAGDAREE
AGASAKLSDDELARIARAFVGTRRALAGRVGMSERTLYRRLRALGIATRDA
>Mature_451_residues
MDQHDVIPIVPVSPPDVGVLVSYLEQDPQPMIVLDPSYRILAANAAYQRQFGIAGEAHVGRRCYQVSHHYDVPCDQAGEH
CPMKQATESRSLNRVLHIHHTPRGPEHVDVELRPIFDAHGAVIAYVERLTTVRSASAKPSGEGLVGGSDAFNAALSALQR
VAPSTLPVLLLGESGTGKELFARALHEASARAMGPFVVVDCSGIAETLFESELFGYEKGAFTGATARKPGLVETAQGGTL
FLDEIGDVPLSMQVKLLRLIESGTFRRVGGVEVLRADFRLVAATHKPLKAMIGDGRFRPDLYYRISAYPIALPAVRERPG
DMPLLVDSILRRIAALGPAAGQRFTVAPDALARLEAYAWPGNIRELRNVLDRACLLTDDGVIRVEHLPDEVARAGDAREE
AGASAKLSDDELARIARAFVGTRRALAGRVGMSERTLYRRLRALGIATRDA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=331, Percent_Identity=41.0876132930514, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788550, Length=301, Percent_Identity=41.5282392026578, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790437, Length=250, Percent_Identity=49.2, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=39.039039039039, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=38.7692307692308, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788905, Length=222, Percent_Identity=45.045045045045, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=35.6948228882834, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI87081872, Length=238, Percent_Identity=44.9579831932773, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082152, Length=250, Percent_Identity=40.8, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1786524, Length=320, Percent_Identity=40, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1787583, Length=374, Percent_Identity=33.4224598930481, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789828, Length=312, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48851; Mature: 48851

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQHDVIPIVPVSPPDVGVLVSYLEQDPQPMIVLDPSYRILAANAAYQRQFGIAGEAHVG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHHHCCCCCCHHHH
RRCYQVSHHYDVPCDQAGEHCPMKQATESRSLNRVLHIHHTPRGPEHVDVELRPIFDAHG
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCEEEEEEEECCCCCC
AVIAYVERLTTVRSASAKPSGEGLVGGSDAFNAALSALQRVAPSTLPVLLLGESGTGKEL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
FARALHEASARAMGPFVVVDCSGIAETLFESELFGYEKGAFTGATARKPGLVETAQGGTL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEE
FLDEIGDVPLSMQVKLLRLIESGTFRRVGGVEVLRADFRLVAATHKPLKAMIGDGRFRPD
EEHHHCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHEEECCCCHHHHHHCCCCCCCC
LYYRISAYPIALPAVRERPGDMPLLVDSILRRIAALGPAAGQRFTVAPDALARLEAYAWP
EEEEEECCEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCC
GNIRELRNVLDRACLLTDDGVIRVEHLPDEVARAGDAREEAGASAKLSDDELARIARAFV
CCHHHHHHHHHHHHEECCCCEEEEECCCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHH
GTRRALAGRVGMSERTLYRRLRALGIATRDA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDQHDVIPIVPVSPPDVGVLVSYLEQDPQPMIVLDPSYRILAANAAYQRQFGIAGEAHVG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHHHCCCCCCHHHH
RRCYQVSHHYDVPCDQAGEHCPMKQATESRSLNRVLHIHHTPRGPEHVDVELRPIFDAHG
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCEEEEEEEECCCCCC
AVIAYVERLTTVRSASAKPSGEGLVGGSDAFNAALSALQRVAPSTLPVLLLGESGTGKEL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
FARALHEASARAMGPFVVVDCSGIAETLFESELFGYEKGAFTGATARKPGLVETAQGGTL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEE
FLDEIGDVPLSMQVKLLRLIESGTFRRVGGVEVLRADFRLVAATHKPLKAMIGDGRFRPD
EEHHHCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHEEECCCCHHHHHHCCCCCCCC
LYYRISAYPIALPAVRERPGDMPLLVDSILRRIAALGPAAGQRFTVAPDALARLEAYAWP
EEEEEECCEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCC
GNIRELRNVLDRACLLTDDGVIRVEHLPDEVARAGDAREEAGASAKLSDDELARIARAFV
CCHHHHHHHHHHHHEECCCCEEEEECCCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHH
GTRRALAGRVGMSERTLYRRLRALGIATRDA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]