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The map label for this gene is tuf-2 [H]

Identifier: 83718741

GI number: 83718741

Start: 3521251

End: 3522441

Strand: Reverse

Name: tuf-2 [H]

Synonym: BTH_I3084

Alternate gene names: 83718741

Gene position: 3522441-3521251 (Counterclockwise)

Preceding gene: 83721077

Following gene: 161723146

Centisome position: 92.47

GC content: 63.48

Gene sequence:

>1191_bases
ATGGCCAAGGAAAAGTTTGAGCGGACCAAGCCGCACGTGAACGTAGGTACGATTGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCGGCGATCGCGACGGTTCTGTCGGCGAAGTTCGGCGGCGAAGCGAAGAAGTACGACGAAATCGACGCGGCGC
CGGAAGAAAAGGCGCGCGGCATCACGATCAACACCGCGCACATCGAGTACGAGACGGCGAACCGCCACTACGCGCACGTT
GATTGCCCGGGCCACGCCGACTACGTGAAGAACATGATCACGGGCGCCGCGCAGATGGACGGCGCGATCCTGGTGTGCTC
GGCCGCGGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCGCGTCAGGTCGGCGTGCCGTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTCGACGACGCCGAGCTGCTCGAGCTGGTCGAAATGGAAGTGCGCGAACTGCTGTCGAAG
TACGACTTCCCGGGCGACGACACGCCGATCATCAAGGGTTCGGCGAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTGGG
CGAAGTGGCGATCATGAATCTGGCCGACGCGCTGGACACGTACATCCCGACGCCGGAGCGCGCGGTGGACGGTGCGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGCCGCGGTACGGTGGTGACGGGTCGTGTCGAGCGCGGCGTGATCAAG
GTCGGCGAAGAAATCGAAATCGTCGGTATCAAGGCGACGGCGAAGACGACCTGCACGGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGCCAGGCGGGCGACAACGTCGGTATCCTGCTGCGCGGCACGAAGCGTGAAGACGTGGAGCGCGGCCAGG
TTCTGGCGAAGCCGGGTTCGATCACGCCGCATACGCACTTCACGGCTGAAGTGTACGTGCTGAGCAAGGACGAAGGCGGC
CGCCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGCTCGATCGAGCTGCC
GAAGGACAAGGAAATGGTGATGCCGGGCGACAACGTGTCGATCACGGTGAAGCTGATCGCGCCGATCGCGATGGAAGAAG
GTCTGCGCTTCGCGATCCGCGAAGGCGGCCGTACCGTCGGCGCCGGCGTCGTCGCCAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
GGGAGAGGTCGGCAGTTCGATCCTGCCCATGGGCACCAGCAGGTAGTCGGTGTCTGTTTGCGCGCGGCGCAACATGTGAA
ATTCCTTTCGGGAGTTGAAA

Downstream 100 bases:

>100_bases
GCCAGTTACTCGTTGATCGACAGTTTGGGGGTCGGCGTTTGCCGATCCCAACATGGTCTAGGGGTATAGCTCAACTGGCA
GAGCGTCGGTCTCCAAAACC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIKATAKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIKATAKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGIKATAKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.1065989847716, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=29.9771167048055, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=29.9771167048055, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.4117647058824, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI46094014, Length=444, Percent_Identity=27.4774774774775, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=28.110599078341, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=28.110599078341, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=28.110599078341, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI54607086, Length=331, Percent_Identity=29.607250755287, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4503507, Length=338, Percent_Identity=27.5147928994083, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=300, Percent_Identity=26, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=29.6187683284457, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=30.5454545454545, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789108, Length=323, Percent_Identity=27.5541795665635, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.605459057072, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.4780361757106, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=30.5010893246187, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=30.5010893246187, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=28.6363636363636, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=31.5018315018315, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=26.2295081967213, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=25.9770114942529, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=28.6118980169972, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.961038961039, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=283, Percent_Identity=26.5017667844523, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.4261603375527, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=537, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.4054054054054, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.4054054054054, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=358, Percent_Identity=26.536312849162, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.7619047619048, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.1698113207547, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=29.6875, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.2697201017812, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.2697201017812, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.8134715025907, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=30.1098901098901, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=30.1098901098901, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=29.8901098901099, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=29.8901098901099, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=29.8901098901099, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=29.8901098901099, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=28.3482142857143, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=30.7471264367816, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.1803278688525, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=27.8810408921933, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=27.8810408921933, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 42992; Mature: 42861

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHIEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIKATAKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHIEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIKATAKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA