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The map label for this gene is rrmJ [H]

Identifier: 83718666

GI number: 83718666

Start: 3190609

End: 3191271

Strand: Reverse

Name: rrmJ [H]

Synonym: BTH_I2777

Alternate gene names: 83718666

Gene position: 3191271-3190609 (Counterclockwise)

Preceding gene: 83720127

Following gene: 83718920

Centisome position: 83.78

GC content: 61.54

Gene sequence:

>663_bases
ATGGCAAAGAACCGCTTTAACCAATCCTGGTTGCACGACCACATCAACGACCCGTACGTCAAAATGGCGCAGCGGGAAGG
GTATCGCGCGCGCGCCGCGTACAAGCTGAAGGAAATCGACGAGCAGGACAAGCTGATCCGTCCGGGCCAGGTGATCGTCG
ATCTGGGCGCGGCGCCGGGGAGCTGGAGCCAGTACGCGCGCAACAAGCTCGCGCAGGGCCGGCGGCGCGACGCGGTGCGC
GAAGGCGGCATCGACGGCACGATCATCGCGCTCGACATGTTGCCGATGGAGCCCATCGCCGACGTCCATTTCATCCAGGG
TGACTTCCGCGAGGAGACGGTGCTGCACCAATTGGAGGAGGTCCTCGCCGGGCGCTCGGTCGACCTTGTAATTTCCGACA
TGGCCCCCAACCTGTCCGGCGTGGCGGTCGCGGACGCGGCGCGGATCGAGCATGTCTGCGATCTGGCGCTCGAATTCGCG
CAAAATCATCTGAAACCGGATGGCGCTCTTTTGGTGAAGTGCTTCCACGGCAGCGGCTACAGCCAGATTGTCGAGAAATT
CAAGCACCAATTTAAAACGGTGGCGCCGCGCAAGCCAAAAGCTTCCCGCGACAAATCGTCCGAAACATTTATTTTGGGTC
GGCATCTGAAGCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CGGCTTGGGCCCGTCGGGGCCGTAAGCCGCGCCAATAAGACCCGCATTATCCGCCAAAAGCGCGGCTTCACGAAGCAATC
GGCAGCAATCTCTTCATCGC

Downstream 100 bases:

>100_bases
AGGTGGCATCGCGCGGCAGGATGCGCCGGAGCCCTTGCCAGCCGTGCCGCGCGCCTATCGGCGCGGTAGCGAATCCTTAT
GGCGAGGGGCTGCGGACTGG

Product: FtsJ-like methyltransferase

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLAQGRRRDAVR
EGGIDGTIIALDMLPMEPIADVHFIQGDFREETVLHQLEEVLAGRSVDLVISDMAPNLSGVAVADAARIEHVCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKLPR

Sequences:

>Translated_220_residues
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLAQGRRRDAVR
EGGIDGTIIALDMLPMEPIADVHFIQGDFREETVLHQLEEVLAGRSVDLVISDMAPNLSGVAVADAARIEHVCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKLPR
>Mature_219_residues
AKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLAQGRRRDAVRE
GGIDGTIIALDMLPMEPIADVHFIQGDFREETVLHQLEEVLAGRSVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQ
NHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKLPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=220, Percent_Identity=35.4545454545455, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=29.064039408867, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=31.5270935960591, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=31.5270935960591, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=31.5270935960591, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=43.8423645320197, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17553474, Length=225, Percent_Identity=32.4444444444444, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17554650, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=31.0344827586207, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=31.0344827586207, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=31.0344827586207, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=30.8080808080808, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=133, Percent_Identity=34.5864661654135, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=33.4862385321101, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648639, Length=206, Percent_Identity=32.0388349514563, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.843137254902, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859957, Length=198, Percent_Identity=29.2929292929293, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24718; Mature: 24586

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGRRRDAVREGGIDGTIIALDMLPMEPIADVHFIQGDFREETVLHQLEE
CHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHH
VLAGRSVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKLPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
AKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGRRRDAVREGGIDGTIIALDMLPMEPIADVHFIQGDFREETVLHQLEE
CHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHH
VLAGRSVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKLPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA