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The map label for this gene is gcvA [H]

Identifier: 83718577

GI number: 83718577

Start: 424902

End: 425864

Strand: Reverse

Name: gcvA [H]

Synonym: BTH_I0383

Alternate gene names: 83718577

Gene position: 425864-424902 (Counterclockwise)

Preceding gene: 83718732

Following gene: 83720695

Centisome position: 11.18

GC content: 70.3

Gene sequence:

>963_bases
ATGAGCATTCGTCAATTGCCGATCCTGAACGCGTTGCGCGTGTTCGAGGCCGCTGCGCGGCACGAGAGCTTTTCGCGGGC
GGCCAACGAGCTGTCCGTCACGCACGGCGCGGTCAGCCATCAGATGCGCGCGCTCGAGGCGGAACTCGGCGTGCCGCTGT
TCGTGCGCCACGGCAAGCGGCTCGCGCTGACGGACGCGGGCGGCCGCTACGCGCAGCAGGTGCGCGCCGCGCTCGCGCTG
CTCGCCGACGCGACCCGCGAGATCCGCGCGAGCGACCGCGACAAGCGGCTCGCCGTGTCGACGCTGCCGTCGTTCGCCGC
GCGCTGGATCACGCCGCGAATCGGCCCGTTCATCGAGCAGCATCCGGAAATCGACCTGGAGCTGCGGGCGAGCGATACGC
TCGTCGATTTCGCGCGCGACGACGTCGACGTCGCGATCCGCTTCGGCTACGGCGTCTATCCGGGACTGTACGTCGAGCCG
CTGCTCGACGAGACGTTTTTCCCCGTCTGCGCGCCGACGCTCAACGGCGGCGTGCTACCCGAGACGCCCGCCGATCTCGT
CCGCTATCCGCTGCTGCGCTCGGACGACGAGCTGTGGCGGCCGTGGTTCGACGCAGCGGGCCTCGATACGCTGACCGAGC
CGAAGCGCGGCGTGCTGTACCAGGATTCGTCGAATCTGCTGCAGGCGGCGATCGACGGCCAGGGCATCGCGCTCGTGCGG
CGCTCGCTCGCGATGCCGGAAGTGGCGGCGGGAAGGATCGTGCGGCTGTTCGACATCGCGGGGCCGAGCCCGTGGCACTA
CTTCTTCGTGTGCCCGCCGTCGCTCGCGCAAACGCCGCGCGTGCAGGCGTTCAGGACGTGGCTGCTGGACGAGATCGCGC
GCTTCAAGGTGCTGTGCGCGACGCAGGAGACGCGGCACGCGGCGGCCTACGCGGCCGCGCGTGCGCTCAAGACAGGGGAT
TGA

Upstream 100 bases:

>100_bases
CAGACTCCGACAGAAGGGGCTTGAATGGCTACAATCGTAGTTCTATCAAGGCCTTGCGCAAAACGATCGATTCTCACGTG
CTTGTGAGAAAAACTAACCG

Downstream 100 bases:

>100_bases
GGCTTCTTTTGTTCAGGCCCTGACGGCCGGCCGTCACAGCACGACCTTGACCATCGGATGGCCCGTCAGCGCGCGGTAGA
TGTTGTCGAGCTGCTCCTGG

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSIRQLPILNALRVFEAAARHESFSRAANELSVTHGAVSHQMRALEAELGVPLFVRHGKRLALTDAGGRYAQQVRAALAL
LADATREIRASDRDKRLAVSTLPSFAARWITPRIGPFIEQHPEIDLELRASDTLVDFARDDVDVAIRFGYGVYPGLYVEP
LLDETFFPVCAPTLNGGVLPETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
RSLAMPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQAFRTWLLDEIARFKVLCATQETRHAAAYAAARALKTGD

Sequences:

>Translated_320_residues
MSIRQLPILNALRVFEAAARHESFSRAANELSVTHGAVSHQMRALEAELGVPLFVRHGKRLALTDAGGRYAQQVRAALAL
LADATREIRASDRDKRLAVSTLPSFAARWITPRIGPFIEQHPEIDLELRASDTLVDFARDDVDVAIRFGYGVYPGLYVEP
LLDETFFPVCAPTLNGGVLPETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
RSLAMPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQAFRTWLLDEIARFKVLCATQETRHAAAYAAARALKTGD
>Mature_319_residues
SIRQLPILNALRVFEAAARHESFSRAANELSVTHGAVSHQMRALEAELGVPLFVRHGKRLALTDAGGRYAQQVRAALALL
ADATREIRASDRDKRLAVSTLPSFAARWITPRIGPFIEQHPEIDLELRASDTLVDFARDDVDVAIRFGYGVYPGLYVEPL
LDETFFPVCAPTLNGGVLPETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVRR
SLAMPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQAFRTWLLDEIARFKVLCATQETRHAAAYAAARALKTGD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=42.8082191780822, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=34.7972972972973, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.6122448979592, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=30.3135888501742, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=30.0699300699301, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=37.5757575757576, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081978, Length=264, Percent_Identity=26.5151515151515, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789440, Length=259, Percent_Identity=24.7104247104247, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=142, Percent_Identity=29.5774647887324, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=207, Percent_Identity=25.6038647342995, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789639, Length=262, Percent_Identity=25.1908396946565, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787806, Length=258, Percent_Identity=29.0697674418605, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788748, Length=167, Percent_Identity=29.940119760479, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35411; Mature: 35280

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRQLPILNALRVFEAAARHESFSRAANELSVTHGAVSHQMRALEAELGVPLFVRHGKR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
LALTDAGGRYAQQVRAALALLADATREIRASDRDKRLAVSTLPSFAARWITPRIGPFIEQ
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHH
HPEIDLELRASDTLVDFARDDVDVAIRFGYGVYPGLYVEPLLDETFFPVCAPTLNGGVLP
CCCCEEEEECCCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
ETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
CCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHH
RSLAMPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQAFRTWLLDEIARFKVLCA
HHHCCCCHHHCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
TQETRHAAAYAAARALKTGD
CHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIRQLPILNALRVFEAAARHESFSRAANELSVTHGAVSHQMRALEAELGVPLFVRHGKR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
LALTDAGGRYAQQVRAALALLADATREIRASDRDKRLAVSTLPSFAARWITPRIGPFIEQ
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHH
HPEIDLELRASDTLVDFARDDVDVAIRFGYGVYPGLYVEPLLDETFFPVCAPTLNGGVLP
CCCCEEEEECCCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
ETPADLVRYPLLRSDDELWRPWFDAAGLDTLTEPKRGVLYQDSSNLLQAAIDGQGIALVR
CCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHH
RSLAMPEVAAGRIVRLFDIAGPSPWHYFFVCPPSLAQTPRVQAFRTWLLDEIARFKVLCA
HHHCCCCHHHCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
TQETRHAAAYAAARALKTGD
CHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]