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The map label for this gene is cca [H]

Identifier: 284159408

GI number: 284159408

Start: 269728

End: 271026

Strand: Direct

Name: cca [H]

Synonym: BTH_I0236

Alternate gene names: 284159408

Gene position: 269728-271026 (Clockwise)

Preceding gene: 83718911

Following gene: 83719578

Centisome position: 7.08

GC content: 72.52

Gene sequence:

>1299_bases
ATGGGTCGAGGGCGCGCGCGGCGAGACCCGCGTGATCGACATCTACGGGCCGTCGCGATGAAGATCTACGCGGTGGGCGG
CGCGATTCGCGACGCACTGCTCGGCCTGCCGGTGCGTGATCGCGACTACGTCGTCGTCGGCGCGACGCCCGAGCGGATGG
CCGCGCAAGGCTTCAAGCCGGTCGGCAAGGATTTTCCGGTGTTCCTGCATCCGGACACGCGCGAGGAATACGCGCTCGCG
CGCACCGAGCGCAAGACGGCGGCCGGCTATCACGGCTTCCAGTTCTATTACGCGCCCGACGTCACGCTCGAGCAGGATCT
CGTGCGGCGCGACCTGACGATCAACGCGATGGCGCGCGAAGTGAGCCCGGACGGCGCGCTCGTCGGGCCGGTCGTCGATC
CGTTCGGCGGGCAGGCGGATTTGCGCGCGAAGCTGTTCCGGCACGTCGGCGGCGCGTTCGTCGAGGACCCGGTGCGGGTG
CTGCGCGTCGCGCGCTTCGCCGCGCGCTTCGCCGATTTTGCGGTTGCGCCCGAGACGTCCGCGCTGATGCGCGAGATGGT
CGACGCGGGCGAGGTCGACGCGCTCGTGCCCGAGCGCGTGTGGCAGGAGCTCGCGCGCGGGCTGATGGAAGCGAAGCCGT
CGCGGATGTTTGCGGTGCTGCGCGAGTGCGGCGCGCTCGTGCGAATCCTGCCCGAGATCGACGCGCTCTTCGGCGTGCCG
CAGCGCGCCGACTATCACCCCGAGGTCGACACGGGCGTGCACGTGATGATGGTGATCGATCACGCGGCGAAGCAGGGCTA
TTCGCTGCCGGTGCGCTTCGCGGCGCTCACGCACGACCTCGGCAAGGCGACGACGCCCGCCGACGCGCTGCCGCGCCACA
TCGGCCACGAGGGCCGCAGCGTCGATCTGCTGAAGCCGCTGTGCGAGCGGCTGCGGGTGCCGAACGAGTGCCGCGATCTG
GCGCTCGTCGTCGCGCGCGAGCACGGCAACCTGCATCGGGTGATGGGGATGGGCGCGGCCGCGCTGGTGCGCCTGTTCGA
GCGCGCCGACGCGCTGCGCAAGCCGGCGCGCTTCGCCGAGGCGCTGCAGGCGAGCGAGGCCGACGCGCGCGGCCGGCTCG
GCCTCGAGACGACGTCCTATCCGCAGGCGGAGCGGCTGCGGCAGGCGCTCGTCGCCGCGCGTTCGGTCGATGCGGGTGCG
ATCGCGCAGGGGCTCGCGGGCGAGCCCGCGAAGATCAAGGATGCGGTGCACCGCGCGCGCGTGCGCGCGGTCGCGCAGGC
GACCGGCGTCGCGGAGTAG

Upstream 100 bases:

>100_bases
ACGCGCCCGTCGTGCTGCGCTTCAAGACCTACGCGCCGCCGCTGTCACCGGAGGCGAGCGCGTATGCCGCGCGCGTCGCC
GAGCTGCCGGCCGTGCGCGA

Downstream 100 bases:

>100_bases
GCGGGTGTCGGGGTTGGCGGGTGTCGGGGTTGGCGGGGCAGGCGCCGGCGCGTTCGGTGCGCGCTTGGGCCGGCGGCAAT
GCGCGCGTCGCGCGCACGCC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGRGRARRDPRDRHLRAVAMKIYAVGGAIRDALLGLPVRDRDYVVVGATPERMAAQGFKPVGKDFPVFLHPDTREEYALA
RTERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQADLRAKLFRHVGGAFVEDPVRV
LRVARFAARFADFAVAPETSALMREMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALVRILPEIDALFGVP
QRADYHPEVDTGVHVMMVIDHAAKQGYSLPVRFAALTHDLGKATTPADALPRHIGHEGRSVDLLKPLCERLRVPNECRDL
ALVVAREHGNLHRVMGMGAAALVRLFERADALRKPARFAEALQASEADARGRLGLETTSYPQAERLRQALVAARSVDAGA
IAQGLAGEPAKIKDAVHRARVRAVAQATGVAE

Sequences:

>Translated_432_residues
MGRGRARRDPRDRHLRAVAMKIYAVGGAIRDALLGLPVRDRDYVVVGATPERMAAQGFKPVGKDFPVFLHPDTREEYALA
RTERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQADLRAKLFRHVGGAFVEDPVRV
LRVARFAARFADFAVAPETSALMREMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALVRILPEIDALFGVP
QRADYHPEVDTGVHVMMVIDHAAKQGYSLPVRFAALTHDLGKATTPADALPRHIGHEGRSVDLLKPLCERLRVPNECRDL
ALVVAREHGNLHRVMGMGAAALVRLFERADALRKPARFAEALQASEADARGRLGLETTSYPQAERLRQALVAARSVDAGA
IAQGLAGEPAKIKDAVHRARVRAVAQATGVAE
>Mature_431_residues
GRGRARRDPRDRHLRAVAMKIYAVGGAIRDALLGLPVRDRDYVVVGATPERMAAQGFKPVGKDFPVFLHPDTREEYALAR
TERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQADLRAKLFRHVGGAFVEDPVRVL
RVARFAARFADFAVAPETSALMREMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALVRILPEIDALFGVPQ
RADYHPEVDTGVHVMMVIDHAAKQGYSLPVRFAALTHDLGKATTPADALPRHIGHEGRSVDLLKPLCERLRVPNECRDLA
LVVAREHGNLHRVMGMGAAALVRLFERADALRKPARFAEALQASEADARGRLGLETTSYPQAERLRQALVAARSVDAGAI
AQGLAGEPAKIKDAVHRARVRAVAQATGVAE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=409, Percent_Identity=54.5232273838631, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87081691, Length=238, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47221; Mature: 47090

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRGRARRDPRDRHLRAVAMKIYAVGGAIRDALLGLPVRDRDYVVVGATPERMAAQGFKP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCC
VGKDFPVFLHPDTREEYALARTERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMARE
CCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHH
VSPDGALVGPVVDPFGGQADLRAKLFRHVGGAFVEDPVRVLRVARFAARFADFAVAPETS
CCCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ALMREMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALVRILPEIDALFGVP
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QRADYHPEVDTGVHVMMVIDHAAKQGYSLPVRFAALTHDLGKATTPADALPRHIGHEGRS
CCCCCCCCCCCCCEEEHEEEHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
VDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMGMGAAALVRLFERADALRKPARFAE
CHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ALQASEADARGRLGLETTSYPQAERLRQALVAARSVDAGAIAQGLAGEPAKIKDAVHRAR
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHH
VRAVAQATGVAE
HHHHHHHCCCCC
>Mature Secondary Structure 
GRGRARRDPRDRHLRAVAMKIYAVGGAIRDALLGLPVRDRDYVVVGATPERMAAQGFKP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCC
VGKDFPVFLHPDTREEYALARTERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMARE
CCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHH
VSPDGALVGPVVDPFGGQADLRAKLFRHVGGAFVEDPVRVLRVARFAARFADFAVAPETS
CCCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ALMREMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALVRILPEIDALFGVP
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QRADYHPEVDTGVHVMMVIDHAAKQGYSLPVRFAALTHDLGKATTPADALPRHIGHEGRS
CCCCCCCCCCCCCEEEHEEEHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
VDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMGMGAAALVRLFERADALRKPARFAE
CHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ALQASEADARGRLGLETTSYPQAERLRQALVAARSVDAGAIAQGLAGEPAKIKDAVHRAR
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHH
VRAVAQATGVAE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA