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The map label for this gene is truA

Identifier: 83591242

GI number: 83591242

Start: 2548407

End: 2549147

Strand: Reverse

Name: truA

Synonym: Moth_2426

Alternate gene names: 83591242

Gene position: 2549147-2548407 (Counterclockwise)

Preceding gene: 83591243

Following gene: 83591241

Centisome position: 96.97

GC content: 61.94

Gene sequence:

>741_bases
ATGCCCTGCCTGAAGATTACCCTGGCCTATGACGGCAGCAATTATGCCGGCTGGCAGGTCCAGCCGGAGGCCCACGGTCC
TACTGTTCAAGGTGAGGTAGCCGCCGCTCTAAAACGGTTGACCGGGGAGGAAATAACTCCGGTAGCTGCCGGGCGAACCG
ACGCCGGGGTCCATGCCCGGGGGCAGGTAATCAGCTTTAGTACCCGGGCCCGGATACCCGTGGAACGCTGGCCCCTGGCC
CTGAACAGCGTCTTGCCGGCAGACATTGCCGCCCTGGAGGCGGTGGAGGTAGCGCCGGATTTCCATGCTCGCTATTGCGC
CAAACGAAAATGGTATCGTTATACCATCTATAACAATCGCGTTCCCGATGTTTTCTGCCGCCGTTATAGCTGGCACTTGC
GGCAGCCCCTCGATGTAATAGCCATGGCCCGGGCGGCGGCTTACCTCCAGGGTTGCCACGATTTCCGCTCCTTTTGCGCC
GCCGGTAGCCCGGTGCGCCACTTTGAGCGGCAGGTGCAGCAGGCCAGCGTCAGTCAGAACGGCCCCTTTATCTATTTTGA
CGTCATAGCCGATGGTTTCCTCTACCATATGGTCCGGATTATGGTCGGCACACTGGTGGAGATCGGCCGCCGTCGCCTGG
TGCCGGAAGCTATCCCGGCCATCCTGGCGGCCCGTTCCCGGGAAAAGGCAGGACCGACGGCCCCGGCCCGTGGACTCTGC
CTGGAGCGCGTGGAATACTAA

Upstream 100 bases:

>100_bases
CGTTATTTTACCGGCTGAAGTCCGGCAACTAGCCGGCCGGGCCGGCGTTTTCCCCAGGCCCCTGGTATTTTCCCTTTCAG
CACGCGGAGGATTACTGGCG

Downstream 100 bases:

>100_bases
TTCCTTGACTTGCAAGGTGTTATTGCTTAAAATATGACTGTTGACAGGAACAGGAGGGAAAAAATGTCCACTTTTATGGC
CAAACCCCATGAGGTTGAGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MPCLKITLAYDGSNYAGWQVQPEAHGPTVQGEVAAALKRLTGEEITPVAAGRTDAGVHARGQVISFSTRARIPVERWPLA
LNSVLPADIAALEAVEVAPDFHARYCAKRKWYRYTIYNNRVPDVFCRRYSWHLRQPLDVIAMARAAAYLQGCHDFRSFCA
AGSPVRHFERQVQQASVSQNGPFIYFDVIADGFLYHMVRIMVGTLVEIGRRRLVPEAIPAILAARSREKAGPTAPARGLC
LERVEY

Sequences:

>Translated_246_residues
MPCLKITLAYDGSNYAGWQVQPEAHGPTVQGEVAAALKRLTGEEITPVAAGRTDAGVHARGQVISFSTRARIPVERWPLA
LNSVLPADIAALEAVEVAPDFHARYCAKRKWYRYTIYNNRVPDVFCRRYSWHLRQPLDVIAMARAAAYLQGCHDFRSFCA
AGSPVRHFERQVQQASVSQNGPFIYFDVIADGFLYHMVRIMVGTLVEIGRRRLVPEAIPAILAARSREKAGPTAPARGLC
LERVEY
>Mature_245_residues
PCLKITLAYDGSNYAGWQVQPEAHGPTVQGEVAAALKRLTGEEITPVAAGRTDAGVHARGQVISFSTRARIPVERWPLAL
NSVLPADIAALEAVEVAPDFHARYCAKRKWYRYTIYNNRVPDVFCRRYSWHLRQPLDVIAMARAAAYLQGCHDFRSFCAA
GSPVRHFERQVQQASVSQNGPFIYFDVIADGFLYHMVRIMVGTLVEIGRRRLVPEAIPAILAARSREKAGPTAPARGLCL
ERVEY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=271, Percent_Identity=33.210332103321, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI199559785, Length=274, Percent_Identity=29.5620437956204, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=42.5101214574899, Blast_Score=176, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321112, Length=236, Percent_Identity=29.6610169491525, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922728, Length=264, Percent_Identity=28.030303030303, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_MOOTA (Q2RFT1)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431251.1
- ProteinModelPortal:   Q2RFT1
- SMR:   Q2RFT1
- STRING:   Q2RFT1
- GeneID:   3832177
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2426
- NMPDR:   fig|264732.9.peg.2459
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q2RFT1
- BioCyc:   MTHE264732:MOTH_2426-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27409; Mature: 27278

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCLKITLAYDGSNYAGWQVQPEAHGPTVQGEVAAALKRLTGEEITPVAAGRTDAGVHAR
CCCEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GQVISFSTRARIPVERWPLALNSVLPADIAALEAVEVAPDFHARYCAKRKWYRYTIYNNR
CCEEEECCCCCCCHHHCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCC
VPDVFCRRYSWHLRQPLDVIAMARAAAYLQGCHDFRSFCAAGSPVRHFERQVQQASVSQN
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
GPFIYFDVIADGFLYHMVRIMVGTLVEIGRRRLVPEAIPAILAARSREKAGPTAPARGLC
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
LERVEY
HHHCCC
>Mature Secondary Structure 
PCLKITLAYDGSNYAGWQVQPEAHGPTVQGEVAAALKRLTGEEITPVAAGRTDAGVHAR
CCEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GQVISFSTRARIPVERWPLALNSVLPADIAALEAVEVAPDFHARYCAKRKWYRYTIYNNR
CCEEEECCCCCCCHHHCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCC
VPDVFCRRYSWHLRQPLDVIAMARAAAYLQGCHDFRSFCAAGSPVRHFERQVQQASVSQN
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
GPFIYFDVIADGFLYHMVRIMVGTLVEIGRRRLVPEAIPAILAARSREKAGPTAPARGLC
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
LERVEY
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA