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The map label for this gene is yhcA [H]

Identifier: 83591145

GI number: 83591145

Start: 2445061

End: 2446665

Strand: Reverse

Name: yhcA [H]

Synonym: Moth_2324

Alternate gene names: 83591145

Gene position: 2446665-2445061 (Counterclockwise)

Preceding gene: 83591146

Following gene: 83591144

Centisome position: 93.07

GC content: 59.88

Gene sequence:

>1605_bases
GTGAAAGTTTTGGCCGATCATGAAAGGGAACAATCGGGGGCAGGAGCAGGCCTGCCGGCAGCCGACGGCGAGGTACAGGG
CTCCTGGATTATCCCCGTCCTGGTGGCCCTCATCGGGGCTTTTATGTCCATCCTGGACTCCAGCATCGTCAATGTGGCCA
TCCCGACCATCATGCATGTTTTTAATACCGATACCAGCACGGTCGAGTGGGTGGTCACCATTTACATGCTAGCGCTGGGG
GTTATTGTCCCCTTAAGCGGCTGGCTGGGTGACAAGCTGGGCTTCAAAAAGTTATACGTCATCGCTCTGGTTATATTTAC
CTTCGGGTCACTCCTGTGCACCTTGAGCTGGAACGTCGATTCCCTCATCGCGGCCCGGGTGGTCCAGGCCCTGGGCGGCG
GTTTGATCATGCCCACTACCATGGCCATGATTTATCGTATGGTACCGCGGGAAAGAATTGGCAGCGCCATGGGAGTGCTG
GGGATTGCCCTCTTTGTGGCGCCGGCCATCGGGCCGACCCTGGGCGGCTACCTGGTGGAGTATGTTGACTGGCGCTGGAT
TTTTACCATTAATCTGCCCATCGGGGTGCTGGGGGTGCTGCTTTCCCTGGTCCTCCTGCCAGATTTCCCGGCTGCCGAAG
CGGGCAGGCTGGATATCGGGGGGGCCGTAACGGCGGCGGTAGGCCTTTTTACCCTCCTCCTGGCCCTGAGCAAGGGCGCG
GACTGGGGCTGGACCTCAGAAGCCACCGTCTTCCTGTTTTACACCAGCGCGGTTTCCCTCGGCCTCTTTATTTACCTGGA
ACTTACCTGTGCCAACCCCCTCCTGGAGCTGAGGGTATTCCGCTATCCGGCCTTTACCCTGGCCAATCTCATGGTGGTGG
TAACCACCATTGGCCTTTTTGGCGGCATTTTCTACGTCCCCCTTTTTCTCCAGACCGTCCGCGGCCTGGGAGCTATGGAA
ACGGGTCTGCTGTCCATGCCCGGCGCCCTGGCCTCGGCGCTGATGATGCCGGTAACCGGCCGCCTCTACGACCGCATCGG
CCCCCGCCTGATGGCGGTGACCGGGCTGGTAGTGCTGGCGATAACAACCTATCTCTTTCACTTCTTAGATATCGTTACCC
CCGACAGGGTCATCATTACCTGGCTGATCCTGCGGAGCGTTAGCATGTCTTTTGCCTCCATGCCGGCCCAGACGGCGGCC
CTGGCGGGGCTGCCGCCAGAACTGGTAGGCCGGGCTTCGGCCATGACCAATATTATCAACCGGGTGTCGGGTTCTTTCGG
GATAGCCATCTTGACCTCGATTTTAAATCACCGTACGGCCCTGCACGCTACGCAGCTAGCAAGCCAGATCACAGCGGACA
ACCCGGCCGTTACGGCCTTTTTCCAGCAGGTGGCTCTCTACCTGGGGAGCGGGTCGGCAGCGACCCAGGTGAAGAGCCTG
GGTACCACTTATCTGGCAGGACTGGTGTCCCAGGCGGCTTTTATACGAGGTATTGACGACATTTTTGTCGTGATGACCGG
TTTTGCCCTGGCCGGCGTCCTCCCGGCCTTTTTCCTCCAAAAAGGGCCTGGCGGCGCCCGGCCGGGCTTTGGCGGCGGCG
AGTAA

Upstream 100 bases:

>100_bases
GGTGAATTGAGGCATATCTTTAATGCCTTGCCGGAAGACTGCAGCCGGCGTTTGCTGGCTGATCTAGAAGTGCTCAATCA
ATACCTGGAACGGGAAGAAG

Downstream 100 bases:

>100_bases
ATGAGAGGAGTGAGCGGTAGTTGTCAAGGAGGTTAGTAGTCATCCTGGTACTCGTTTTGGCCCTGGCTGGTGCCGGTGCC
ATTGCCTTTTATTACTACTA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKVLADHEREQSGAGAGLPAADGEVQGSWIIPVLVALIGAFMSILDSSIVNVAIPTIMHVFNTDTSTVEWVVTIYMLALG
VIVPLSGWLGDKLGFKKLYVIALVIFTFGSLLCTLSWNVDSLIAARVVQALGGGLIMPTTMAMIYRMVPRERIGSAMGVL
GIALFVAPAIGPTLGGYLVEYVDWRWIFTINLPIGVLGVLLSLVLLPDFPAAEAGRLDIGGAVTAAVGLFTLLLALSKGA
DWGWTSEATVFLFYTSAVSLGLFIYLELTCANPLLELRVFRYPAFTLANLMVVVTTIGLFGGIFYVPLFLQTVRGLGAME
TGLLSMPGALASALMMPVTGRLYDRIGPRLMAVTGLVVLAITTYLFHFLDIVTPDRVIITWLILRSVSMSFASMPAQTAA
LAGLPPELVGRASAMTNIINRVSGSFGIAILTSILNHRTALHATQLASQITADNPAVTAFFQQVALYLGSGSAATQVKSL
GTTYLAGLVSQAAFIRGIDDIFVVMTGFALAGVLPAFFLQKGPGGARPGFGGGE

Sequences:

>Translated_534_residues
MKVLADHEREQSGAGAGLPAADGEVQGSWIIPVLVALIGAFMSILDSSIVNVAIPTIMHVFNTDTSTVEWVVTIYMLALG
VIVPLSGWLGDKLGFKKLYVIALVIFTFGSLLCTLSWNVDSLIAARVVQALGGGLIMPTTMAMIYRMVPRERIGSAMGVL
GIALFVAPAIGPTLGGYLVEYVDWRWIFTINLPIGVLGVLLSLVLLPDFPAAEAGRLDIGGAVTAAVGLFTLLLALSKGA
DWGWTSEATVFLFYTSAVSLGLFIYLELTCANPLLELRVFRYPAFTLANLMVVVTTIGLFGGIFYVPLFLQTVRGLGAME
TGLLSMPGALASALMMPVTGRLYDRIGPRLMAVTGLVVLAITTYLFHFLDIVTPDRVIITWLILRSVSMSFASMPAQTAA
LAGLPPELVGRASAMTNIINRVSGSFGIAILTSILNHRTALHATQLASQITADNPAVTAFFQQVALYLGSGSAATQVKSL
GTTYLAGLVSQAAFIRGIDDIFVVMTGFALAGVLPAFFLQKGPGGARPGFGGGE
>Mature_534_residues
MKVLADHEREQSGAGAGLPAADGEVQGSWIIPVLVALIGAFMSILDSSIVNVAIPTIMHVFNTDTSTVEWVVTIYMLALG
VIVPLSGWLGDKLGFKKLYVIALVIFTFGSLLCTLSWNVDSLIAARVVQALGGGLIMPTTMAMIYRMVPRERIGSAMGVL
GIALFVAPAIGPTLGGYLVEYVDWRWIFTINLPIGVLGVLLSLVLLPDFPAAEAGRLDIGGAVTAAVGLFTLLLALSKGA
DWGWTSEATVFLFYTSAVSLGLFIYLELTCANPLLELRVFRYPAFTLANLMVVVTTIGLFGGIFYVPLFLQTVRGLGAME
TGLLSMPGALASALMMPVTGRLYDRIGPRLMAVTGLVVLAITTYLFHFLDIVTPDRVIITWLILRSVSMSFASMPAQTAA
LAGLPPELVGRASAMTNIINRVSGSFGIAILTSILNHRTALHATQLASQITADNPAVTAFFQQVALYLGSGSAATQVKSL
GTTYLAGLVSQAAFIRGIDDIFVVMTGFALAGVLPAFFLQKGPGGARPGFGGGE

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=490, Percent_Identity=29.3877551020408, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788710, Length=450, Percent_Identity=30.8888888888889, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=31.0344827586207, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=29.0476190476191, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788392, Length=394, Percent_Identity=32.48730964467, Blast_Score=165, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6322958, Length=355, Percent_Identity=23.943661971831, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=322, Percent_Identity=26.0869565217391, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=138, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=404, Percent_Identity=25.2475247524752, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=32.6666666666667, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=350, Percent_Identity=26.2857142857143, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56416; Mature: 56416

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLADHEREQSGAGAGLPAADGEVQGSWIIPVLVALIGAFMSILDSSIVNVAIPTIMHV
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNTDTSTVEWVVTIYMLALGVIVPLSGWLGDKLGFKKLYVIALVIFTFGSLLCTLSWNVD
HCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
SLIAARVVQALGGGLIMPTTMAMIYRMVPRERIGSAMGVLGIALFVAPAIGPTLGGYLVE
HHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
YVDWRWIFTINLPIGVLGVLLSLVLLPDFPAAEAGRLDIGGAVTAAVGLFTLLLALSKGA
HHCEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DWGWTSEATVFLFYTSAVSLGLFIYLELTCANPLLELRVFRYPAFTLANLMVVVTTIGLF
CCCCCCCCEEEEEEHHHHHHHHHHEEEHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
GGIFYVPLFLQTVRGLGAMETGLLSMPGALASALMMPVTGRLYDRIGPRLMAVTGLVVLA
HHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
ITTYLFHFLDIVTPDRVIITWLILRSVSMSFASMPAQTAALAGLPPELVGRASAMTNIIN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHH
RVSGSFGIAILTSILNHRTALHATQLASQITADNPAVTAFFQQVALYLGSGSAATQVKSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
GTTYLAGLVSQAAFIRGIDDIFVVMTGFALAGVLPAFFLQKGPGGARPGFGGGE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVLADHEREQSGAGAGLPAADGEVQGSWIIPVLVALIGAFMSILDSSIVNVAIPTIMHV
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNTDTSTVEWVVTIYMLALGVIVPLSGWLGDKLGFKKLYVIALVIFTFGSLLCTLSWNVD
HCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
SLIAARVVQALGGGLIMPTTMAMIYRMVPRERIGSAMGVLGIALFVAPAIGPTLGGYLVE
HHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
YVDWRWIFTINLPIGVLGVLLSLVLLPDFPAAEAGRLDIGGAVTAAVGLFTLLLALSKGA
HHCEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DWGWTSEATVFLFYTSAVSLGLFIYLELTCANPLLELRVFRYPAFTLANLMVVVTTIGLF
CCCCCCCCEEEEEEHHHHHHHHHHEEEHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
GGIFYVPLFLQTVRGLGAMETGLLSMPGALASALMMPVTGRLYDRIGPRLMAVTGLVVLA
HHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
ITTYLFHFLDIVTPDRVIITWLILRSVSMSFASMPAQTAALAGLPPELVGRASAMTNIIN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHH
RVSGSFGIAILTSILNHRTALHATQLASQITADNPAVTAFFQQVALYLGSGSAATQVKSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
GTTYLAGLVSQAAFIRGIDDIFVVMTGFALAGVLPAFFLQKGPGGARPGFGGGE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]