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The map label for this gene is macA [H]

Identifier: 83591143

GI number: 83591143

Start: 2443089

End: 2444288

Strand: Reverse

Name: macA [H]

Synonym: Moth_2322

Alternate gene names: 83591143

Gene position: 2444288-2443089 (Counterclockwise)

Preceding gene: 83591144

Following gene: 83591142

Centisome position: 92.98

GC content: 62.0

Gene sequence:

>1200_bases
ATGCCAATGTGGGGGAAAAGGGTGGTAGCCCTTTTGCTTGTAGGTGTTCTGGCCCTGGGTGTCGCCGGTTGCGGCGCCAG
GGCCGGGGCCAGCGACCAGGTGACTGTTAAGGCTGTCGCCGCCAGCAAGGGGAAGATGGCGGCGACGGTAGAGGTAACCG
GCGCCCTGGTGCCGGCCCGGACGGCCAATGTGGTCAGCAAACTGTCCGGTCAGGTGACGGCCGTGAAAGCTGATGTCGGC
GACCGGGTGCGGGCCGGCCAGGTCCTGGTGGAGATTGATACCAAGGAGCTCCAGGCCCAGCTCCAGCAGGCCGAGGCTGC
CGTTCGCAGTGTCCAGGATCAGGCGGAACAGGCGCGCATCGGTATGGATTCAGCCCGCGTCGCTATAGCCAATGCCAAGG
TCGCATTGGATGCCGCCCAGAAGTACTACGATCGTATTAAAGCCCTGGCAGATGCCGGCGCGGCGTCCCAGAGCCAGCTG
GACGATGCCCAGACCAAGTTGGACCAGGCTAAAAATGGCTATGAGGCTGCCAACAAACAGTATGAGGCCGCCCAAAAACA
ATATGAAACCGCATCCGGGTCCGGTTTGGCCCAGGCTGAAGCGGCGGTCAACATTATTAAAGTAAATATGAGTAATGCCA
GCATCACCAGTCCCATCACCGGTGTTGTCACCAACCGGAACATAAATCCCGGTGAAATGGCCGCACCCACGAGCCCCCAG
CCCCTGCTGACCATCGCCGATACTTCCACCCTGAAGCTCCAGGGGACAGTCGGCCAGGAGGCAGTCCCCCTGCTGTCAAC
GGGACAGAAGGTGACAGTCACCCTGGACGCCCTGCCGGGCCGGGAGTTTACCGGCACGGTGACCCAGGTGGGCCCGGTGG
CGGCTACCACCGGCCAGCGTTTCCCGGTGGAGATCAGCCTGCCCAACCCGGGCGAGCTGAAGGCCGGCATGACGGCCCGG
GCCGTATTTAAGCTGGCGGCCCCGGAGGGCGTCGTTGTACCCCTTGCTGCTGTTCGCACGGACAACGGCCAGGATTATGT
CTTTGTGGTTAAGAACGGCACGGTAGAGCGGCGGCAGGTTGTTCTGGGCCTGAAAAACGATGAACAGGTGATGATACTTA
AGGGGCTGCAGGCCGGCGAGCAGGTGGCCGTGACCAACGTAGGCGTGCTGCAGGATAAGATGGCAGTGACAGTGGAGTAA

Upstream 100 bases:

>100_bases
TGCTGCCGGGGATGAATGCCACGGTACGCATTCATATTAGATAATTACGGGAAAATATGCGGTTGAACGGCAAGTTATTC
ATTGTGGAGGGGAGCAAGCT

Downstream 100 bases:

>100_bases
TGTTAGTCCAGACGGGATTGACTGGATAATGGGCTAATGTTAGGATGTAAAGTAAAGAAATACTATTGGTAAAACATTTT
TGGTCAAGAGAGGGGAAGGT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPMWGKRVVALLLVGVLALGVAGCGARAGASDQVTVKAVAASKGKMAATVEVTGALVPARTANVVSKLSGQVTAVKADVG
DRVRAGQVLVEIDTKELQAQLQQAEAAVRSVQDQAEQARIGMDSARVAIANAKVALDAAQKYYDRIKALADAGAASQSQL
DDAQTKLDQAKNGYEAANKQYEAAQKQYETASGSGLAQAEAAVNIIKVNMSNASITSPITGVVTNRNINPGEMAAPTSPQ
PLLTIADTSTLKLQGTVGQEAVPLLSTGQKVTVTLDALPGREFTGTVTQVGPVAATTGQRFPVEISLPNPGELKAGMTAR
AVFKLAAPEGVVVPLAAVRTDNGQDYVFVVKNGTVERRQVVLGLKNDEQVMILKGLQAGEQVAVTNVGVLQDKMAVTVE

Sequences:

>Translated_399_residues
MPMWGKRVVALLLVGVLALGVAGCGARAGASDQVTVKAVAASKGKMAATVEVTGALVPARTANVVSKLSGQVTAVKADVG
DRVRAGQVLVEIDTKELQAQLQQAEAAVRSVQDQAEQARIGMDSARVAIANAKVALDAAQKYYDRIKALADAGAASQSQL
DDAQTKLDQAKNGYEAANKQYEAAQKQYETASGSGLAQAEAAVNIIKVNMSNASITSPITGVVTNRNINPGEMAAPTSPQ
PLLTIADTSTLKLQGTVGQEAVPLLSTGQKVTVTLDALPGREFTGTVTQVGPVAATTGQRFPVEISLPNPGELKAGMTAR
AVFKLAAPEGVVVPLAAVRTDNGQDYVFVVKNGTVERRQVVLGLKNDEQVMILKGLQAGEQVAVTNVGVLQDKMAVTVE
>Mature_398_residues
PMWGKRVVALLLVGVLALGVAGCGARAGASDQVTVKAVAASKGKMAATVEVTGALVPARTANVVSKLSGQVTAVKADVGD
RVRAGQVLVEIDTKELQAQLQQAEAAVRSVQDQAEQARIGMDSARVAIANAKVALDAAQKYYDRIKALADAGAASQSQLD
DAQTKLDQAKNGYEAANKQYEAAQKQYETASGSGLAQAEAAVNIIKVNMSNASITSPITGVVTNRNINPGEMAAPTSPQP
LLTIADTSTLKLQGTVGQEAVPLLSTGQKVTVTLDALPGREFTGTVTQVGPVAATTGQRFPVEISLPNPGELKAGMTARA
VFKLAAPEGVVVPLAAVRTDNGQDYVFVVKNGTVERRQVVLGLKNDEQVMILKGLQAGEQVAVTNVGVLQDKMAVTVE

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=359, Percent_Identity=27.8551532033426, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789041, Length=278, Percent_Identity=27.3381294964029, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787013, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789900, Length=310, Percent_Identity=27.0967741935484, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789929, Length=398, Percent_Identity=24.1206030150754, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790519, Length=300, Percent_Identity=23.6666666666667, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41303; Mature: 41171

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMWGKRVVALLLVGVLALGVAGCGARAGASDQVTVKAVAASKGKMAATVEVTGALVPAR
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECEECCCH
TANVVSKLSGQVTAVKADVGDRVRAGQVLVEIDTKELQAQLQQAEAAVRSVQDQAEQARI
HHHHHHHHCCCEEEEEECCCCCEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMDSARVAIANAKVALDAAQKYYDRIKALADAGAASQSQLDDAQTKLDQAKNGYEAANKQ
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
YEAAQKQYETASGSGLAQAEAAVNIIKVNMSNASITSPITGVVTNRNINPGEMAAPTSPQ
HHHHHHHHHCCCCCCCHHHCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PLLTIADTSTLKLQGTVGQEAVPLLSTGQKVTVTLDALPGREFTGTVTQVGPVAATTGQR
CEEEEECCCEEEEEECCCCCCCCHHCCCCEEEEEEECCCCCCCCCCCEECCCEEECCCCC
FPVEISLPNPGELKAGMTARAVFKLAAPEGVVVPLAAVRTDNGQDYVFVVKNGTVERRQV
CEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEEEECCCCEEEEE
VLGLKNDEQVMILKGLQAGEQVAVTNVGVLQDKMAVTVE
EEEECCCCEEEEEECCCCCCEEEEEECCEECCCEEEEEC
>Mature Secondary Structure 
PMWGKRVVALLLVGVLALGVAGCGARAGASDQVTVKAVAASKGKMAATVEVTGALVPAR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECEECCCH
TANVVSKLSGQVTAVKADVGDRVRAGQVLVEIDTKELQAQLQQAEAAVRSVQDQAEQARI
HHHHHHHHCCCEEEEEECCCCCEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMDSARVAIANAKVALDAAQKYYDRIKALADAGAASQSQLDDAQTKLDQAKNGYEAANKQ
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
YEAAQKQYETASGSGLAQAEAAVNIIKVNMSNASITSPITGVVTNRNINPGEMAAPTSPQ
HHHHHHHHHCCCCCCCHHHCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PLLTIADTSTLKLQGTVGQEAVPLLSTGQKVTVTLDALPGREFTGTVTQVGPVAATTGQR
CEEEEECCCEEEEEECCCCCCCCHHCCCCEEEEEEECCCCCCCCCCCEECCCEEECCCCC
FPVEISLPNPGELKAGMTARAVFKLAAPEGVVVPLAAVRTDNGQDYVFVVKNGTVERRQV
CEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEEEECCCCEEEEE
VLGLKNDEQVMILKGLQAGEQVAVTNVGVLQDKMAVTVE
EEEECCCCEEEEEECCCCCCEEEEEECCEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]