Click here to switch to the map view.

The map label for this gene is fumA [H]

Identifier: 83591103

GI number: 83591103

Start: 2393727

End: 2394386

Strand: Reverse

Name: fumA [H]

Synonym: Moth_2282

Alternate gene names: 83591103

Gene position: 2394386-2393727 (Counterclockwise)

Preceding gene: 83591104

Following gene: 83591102

Centisome position: 91.08

GC content: 61.67

Gene sequence:

>660_bases
ATGGAGGTTAGCCATCCAAGCACCCAGCCGGCACCAGGTAGTTCCTTGAAACGCCTGGTAGGACCGGCCGGAGATAATAG
GCGGACGGGGGATTCTCCCATCAGACATCTCCATACACCCCTCAGTGTTGACGATGTCGAGTCCCTCCGGGCCGGCGACC
GGGTCTTAATCAGCGGCGTTATCTACGCCGCCCGGGACAGTGCCCATAAAAGGCTGGTTGAACTCCTGGACCGGGGTGAA
GAACTCCCCGTCGACCTCAAGGGACAGGTTATTTACTACGTCGGCCCTACACCGGCCAGACCCGGCCGGGTTACCGGCGC
GGCCGGGCCCACCACCAGCGGCCGCATGGACCCCTATACCCCGCGGTTAATCGCCGCCACCGGCCTCAAGGGGATGATCG
GCAAGGGTTTCCGCTCACCTGAAGTCAAGAAGGCCCTGGTGGACCATAAGGCTGTCTACTTCGCGGCCGTGGGCGGCGCC
GGAGCTTTAATCGCCAGGTGCATCAAGAAGGCGGAAATAGTGGCCTACCCCGAGCTGGGGCCCGAGGCCCTGCGTGCCCT
GGAGGTCGAGGACCTGCCGGTTACCGTAATCAACGACTGCTACGGCGGGGATCTCTATACTGAAGCCCTGGCCAGGTATA
GTATAAAGGAAGTTCCTTAA

Upstream 100 bases:

>100_bases
AACCGTTCCACCATCGTTCCCGCGGGAATCTGCTCCTTGGAGACCACGGCGATGAAATAGTATTTACTTTCTGGCCATAT
CATAAGGGGGAGGCCTGCAG

Downstream 100 bases:

>100_bases
ACGGTGAATATTATTCTGGCCTGCACTCACCCGGACCATCTTTTTTATTCTGGCTTTAATACATTTAAAGTTGGCCAAAC
AGGCAGGTAACCAATAGAAT

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MEVSHPSTQPAPGSSLKRLVGPAGDNRRTGDSPIRHLHTPLSVDDVESLRAGDRVLISGVIYAARDSAHKRLVELLDRGE
ELPVDLKGQVIYYVGPTPARPGRVTGAAGPTTSGRMDPYTPRLIAATGLKGMIGKGFRSPEVKKALVDHKAVYFAAVGGA
GALIARCIKKAEIVAYPELGPEALRALEVEDLPVTVINDCYGGDLYTEALARYSIKEVP

Sequences:

>Translated_219_residues
MEVSHPSTQPAPGSSLKRLVGPAGDNRRTGDSPIRHLHTPLSVDDVESLRAGDRVLISGVIYAARDSAHKRLVELLDRGE
ELPVDLKGQVIYYVGPTPARPGRVTGAAGPTTSGRMDPYTPRLIAATGLKGMIGKGFRSPEVKKALVDHKAVYFAAVGGA
GALIARCIKKAEIVAYPELGPEALRALEVEDLPVTVINDCYGGDLYTEALARYSIKEVP
>Mature_219_residues
MEVSHPSTQPAPGSSLKRLVGPAGDNRRTGDSPIRHLHTPLSVDDVESLRAGDRVLISGVIYAARDSAHKRLVELLDRGE
ELPVDLKGQVIYYVGPTPARPGRVTGAAGPTTSGRMDPYTPRLIAATGLKGMIGKGFRSPEVKKALVDHKAVYFAAVGGA
GALIARCIKKAEIVAYPELGPEALRALEVEDLPVTVINDCYGGDLYTEALARYSIKEVP

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=160, Percent_Identity=41.875, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787897, Length=156, Percent_Identity=42.3076923076923, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1789443, Length=176, Percent_Identity=32.9545454545455, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 23318; Mature: 23318

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVSHPSTQPAPGSSLKRLVGPAGDNRRTGDSPIRHLHTPLSVDDVESLRAGDRVLISGV
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCEEEEHHH
IYAARDSAHKRLVELLDRGEELPVDLKGQVIYYVGPTPARPGRVTGAAGPTTSGRMDPYT
HHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCC
PRLIAATGLKGMIGKGFRSPEVKKALVDHKAVYFAAVGGAGALIARCIKKAEIVAYPELG
CCEEECCCCHHHHCCCCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHEEECCCCC
PEALRALEVEDLPVTVINDCYGGDLYTEALARYSIKEVP
HHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVSHPSTQPAPGSSLKRLVGPAGDNRRTGDSPIRHLHTPLSVDDVESLRAGDRVLISGV
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCEEEEHHH
IYAARDSAHKRLVELLDRGEELPVDLKGQVIYYVGPTPARPGRVTGAAGPTTSGRMDPYT
HHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCC
PRLIAATGLKGMIGKGFRSPEVKKALVDHKAVYFAAVGGAGALIARCIKKAEIVAYPELG
CCEEECCCCHHHHCCCCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHEEECCCCC
PEALRALEVEDLPVTVINDCYGGDLYTEALARYSIKEVP
HHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]