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The map label for this gene is ykfC [H]

Identifier: 83591045

GI number: 83591045

Start: 2318855

End: 2319784

Strand: Reverse

Name: ykfC [H]

Synonym: Moth_2224

Alternate gene names: 83591045

Gene position: 2319784-2318855 (Counterclockwise)

Preceding gene: 83591046

Following gene: 83591043

Centisome position: 88.25

GC content: 61.18

Gene sequence:

>930_bases
ATGTTTAAGGGTGGGCGTTATATAAGCATCTATTATTTTATTATAGCTCTGGCCATGGTTATAGCAGGGGGATTCCTGTT
GAGCAGGCAGGCGAAAAGGTTGCCGCCACCGGTACAACTTCCCCCCGGGGCGACAACTCCCCGGGCGGAAAGCTGGTATG
TGGGGGTAGCGGTGGCTGACGTGCGGGCTAACCCGGATCAGGGCGCCGAACGGGTTACCCAGGCACTCCTGGGGGATGAG
GTAAAACTCCTGCGGGACGAAGGCGAATGGCTCCAGGGTCAGGTGCCTGATGGTTACATCGGCTGGTTGCAAAAGGGGAA
CCTGGTCAGGGCGACGCCCCCGCTGGCCCGGGACCTGGTGGCCGTCAGGGTGCCCAGGGCCATATTATACAAGGAGCCGG
GGAGCGATGCTCAAGCCGGGGAGGCCTTGCTGGGCACTGACCTGCCCCTGCTGGCGCAAAAGGAGGATTGGCTGGAGGTC
TGGCTGCCGGGCCGCCCGCCAGCCTGGCTTAGCCGGCAGGAAGTCGACCTCTGGCCCGGGGGTCAATTAACGGATAAGCG
TTCCGGTTCGGACGTGATTAAGGTGGCCGAACGGTTGGAGGGGGTGGCCTACCTGTGGGGTGGGGTCAGCCTCTACGGTA
TCGACTGCTCCGGCCTGACCTATATAGCCTATTTCTTAAACGGCGTTAAGCTGCCCCGGGATGCCGACCTGCAGTTTAAA
GTCGGCCGGCCTGTAGCCCGGAAAGACCTGCAGCCCGGCGATCTGGTCTTTTTTAATACCAGTGGCGGAACGCAGCCTAC
CCACGTTGGTATCTATACAGGCAACGGGCAGTTTTTAAACTCACGTTCCCGCCAGGGGGTGGTTGTCAGCCGCCTGGATG
AGCCCTCTTTTAGCGCCGGGTATCTGGGCGCCCGCCGGTACCTGCCGTGA

Upstream 100 bases:

>100_bases
AGCATTTTAAAAATAAAGCCGGAGGTTAGACGGCAAAAAGGGGTATGATAAAAAAGCGGTGACAGTGAGTTTTTGAATCT
TTATCAATAAAGGAGCATAG

Downstream 100 bases:

>100_bases
TATGGGGCCTGGTAGCTGGAACTCAAACCGGCATGTTGCTGTATCATAAATAATATCAAGCTGCATGGCAGGACCACGGG
CTGAATGTTGCCCGTGGCCC

Product: NLP/P60

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MFKGGRYISIYYFIIALAMVIAGGFLLSRQAKRLPPPVQLPPGATTPRAESWYVGVAVADVRANPDQGAERVTQALLGDE
VKLLRDEGEWLQGQVPDGYIGWLQKGNLVRATPPLARDLVAVRVPRAILYKEPGSDAQAGEALLGTDLPLLAQKEDWLEV
WLPGRPPAWLSRQEVDLWPGGQLTDKRSGSDVIKVAERLEGVAYLWGGVSLYGIDCSGLTYIAYFLNGVKLPRDADLQFK
VGRPVARKDLQPGDLVFFNTSGGTQPTHVGIYTGNGQFLNSRSRQGVVVSRLDEPSFSAGYLGARRYLP

Sequences:

>Translated_309_residues
MFKGGRYISIYYFIIALAMVIAGGFLLSRQAKRLPPPVQLPPGATTPRAESWYVGVAVADVRANPDQGAERVTQALLGDE
VKLLRDEGEWLQGQVPDGYIGWLQKGNLVRATPPLARDLVAVRVPRAILYKEPGSDAQAGEALLGTDLPLLAQKEDWLEV
WLPGRPPAWLSRQEVDLWPGGQLTDKRSGSDVIKVAERLEGVAYLWGGVSLYGIDCSGLTYIAYFLNGVKLPRDADLQFK
VGRPVARKDLQPGDLVFFNTSGGTQPTHVGIYTGNGQFLNSRSRQGVVVSRLDEPSFSAGYLGARRYLP
>Mature_309_residues
MFKGGRYISIYYFIIALAMVIAGGFLLSRQAKRLPPPVQLPPGATTPRAESWYVGVAVADVRANPDQGAERVTQALLGDE
VKLLRDEGEWLQGQVPDGYIGWLQKGNLVRATPPLARDLVAVRVPRAILYKEPGSDAQAGEALLGTDLPLLAQKEDWLEV
WLPGRPPAWLSRQEVDLWPGGQLTDKRSGSDVIKVAERLEGVAYLWGGVSLYGIDCSGLTYIAYFLNGVKLPRDADLQFK
VGRPVARKDLQPGDLVFFNTSGGTQPTHVGIYTGNGQFLNSRSRQGVVVSRLDEPSFSAGYLGARRYLP

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=113, Percent_Identity=42.4778761061947, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786421, Length=180, Percent_Identity=32.2222222222222, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788501, Length=113, Percent_Identity=41.5929203539823, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788001, Length=108, Percent_Identity=37.037037037037, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 33790; Mature: 33790

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGGRYISIYYFIIALAMVIAGGFLLSRQAKRLPPPVQLPPGATTPRAESWYVGVAVAD
CCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEE
VRANPDQGAERVTQALLGDEVKLLRDEGEWLQGQVPDGYIGWLQKGNLVRATPPLARDLV
ECCCCCHHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHCCCCEEEECCCHHHHHH
AVRVPRAILYKEPGSDAQAGEALLGTDLPLLAQKEDWLEVWLPGRPPAWLSRQEVDLWPG
HHHCCCEEEEECCCCCHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCC
GQLTDKRSGSDVIKVAERLEGVAYLWGGVSLYGIDCSGLTYIAYFLNGVKLPRDADLQFK
CCCCCCCCCHHHHHHHHHHCCEEEEECCEEEEEECCCCHHHHHHHHHCCCCCCCCCCEEE
VGRPVARKDLQPGDLVFFNTSGGTQPTHVGIYTGNGQFLNSRSRQGVVVSRLDEPSFSAG
CCCCHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCCC
YLGARRYLP
CCCCCCCCC
>Mature Secondary Structure
MFKGGRYISIYYFIIALAMVIAGGFLLSRQAKRLPPPVQLPPGATTPRAESWYVGVAVAD
CCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEE
VRANPDQGAERVTQALLGDEVKLLRDEGEWLQGQVPDGYIGWLQKGNLVRATPPLARDLV
ECCCCCHHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHCCCCEEEECCCHHHHHH
AVRVPRAILYKEPGSDAQAGEALLGTDLPLLAQKEDWLEVWLPGRPPAWLSRQEVDLWPG
HHHCCCEEEEECCCCCHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCC
GQLTDKRSGSDVIKVAERLEGVAYLWGGVSLYGIDCSGLTYIAYFLNGVKLPRDADLQFK
CCCCCCCCCHHHHHHHHHHCCEEEEECCEEEEEECCCCHHHHHHHHHCCCCCCCCCCEEE
VGRPVARKDLQPGDLVFFNTSGGTQPTHVGIYTGNGQFLNSRSRQGVVVSRLDEPSFSAG
CCCCHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCCC
YLGARRYLP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]