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The map label for this gene is gcvH [H]

Identifier: 83591041

GI number: 83591041

Start: 2314109

End: 2314981

Strand: Reverse

Name: gcvH [H]

Synonym: Moth_2218

Alternate gene names: 83591041

Gene position: 2314981-2314109 (Counterclockwise)

Preceding gene: 83591042

Following gene: 83591040

Centisome position: 88.06

GC content: 56.7

Gene sequence:

>873_bases
GTGACTGCAAAGTATGTTATCTTGCCCTGTAACGGCCTGGATAAAGAAGCCGGCTGCCTGGCCCGGGAACTGGCCCTGAA
AATGGCGGCGGCCACCGGGAGCGAGATTATCTGCCCGGTGCTTTACCAGACGGCGCCGTCCCGTTATGCTTCTCTTCTCC
GGGAAGGTAGCCTGGTGGTTATCGACGGCTGCGCCACCCGTTGCGCCAGCCGGATTGCCGCCAACAATAACCTGAAGATT
TACCGCAAGATAACTATGACCGAAGAAGCTAAAAAGAGGGATTATAACCCCGGCCCGGACTGGCGGGTGGGGGCAGGGGC
GGCGGCCTTCGTGGAGGATGTCTGGCGGTCCTGGCAGCCTGTACTCAGGGAACAGGAAGCCGGCCGGGCGCCGGGAGAAA
ACGCCGGCCTTTTTGCCGGCCCGCTGGAATACGCCATCCATCGCCACGATAAGTTTATCTTCCGGGTACCCCTGGAAGGC
TTTTACTTCAACGAGAACGACTGCTGGGTGCAAGTAGAGGGGAACAGGGGCCGCGTAGGCATCAGCGACTACCTGCAGCA
AAACCTTTCCGATATTACCTTTGTCACGCCCCCCGATCCGGGTACGGAAGTGGAGCAATTCGGCGAAATGGGTACCATCG
AGTCGGCCAAAGCGGTTTATGAACTGGTTTCCCCGGTTACAGGCAGGGTGGTGGCCGTTAATGAAGCCATCCTAGAGGCG
CCGGAACTCATCAATGAAAACCCCTATGAAAAGGGCTGGATTACCGAACTGGAACTGACCAATTTTGAGGCTGACCGCGA
ATTTTTACTGGACGGGCGGCGCTATATGGAAGTATTAAAGGAAAAGGTGGCTGATTTCAATGCCGGTAAATAG

Upstream 100 bases:

>100_bases
GCGCCAAGACCTGCGGGCCGGATGCCATTTCCTTTCCCGAAAAATCCCGGGTCCTGGAAGCAATCAAGGCCCTGCGCCAG
GAAGAAGGGGGTAAATAGCC

Downstream 100 bases:

>100_bases
CTCCCGCAAAGTTTACGTCATCCCCTGCAGCGGTATCGGCAAGATGTACGGCCTCCTGGGCCGGGAAGCAGTACTGAAGA
CTGTAAAAGAGCTGCGGCCC

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTAKYVILPCNGLDKEAGCLARELALKMAAATGSEIICPVLYQTAPSRYASLLREGSLVVIDGCATRCASRIAANNNLKI
YRKITMTEEAKKRDYNPGPDWRVGAGAAAFVEDVWRSWQPVLREQEAGRAPGENAGLFAGPLEYAIHRHDKFIFRVPLEG
FYFNENDCWVQVEGNRGRVGISDYLQQNLSDITFVTPPDPGTEVEQFGEMGTIESAKAVYELVSPVTGRVVAVNEAILEA
PELINENPYEKGWITELELTNFEADREFLLDGRRYMEVLKEKVADFNAGK

Sequences:

>Translated_290_residues
MTAKYVILPCNGLDKEAGCLARELALKMAAATGSEIICPVLYQTAPSRYASLLREGSLVVIDGCATRCASRIAANNNLKI
YRKITMTEEAKKRDYNPGPDWRVGAGAAAFVEDVWRSWQPVLREQEAGRAPGENAGLFAGPLEYAIHRHDKFIFRVPLEG
FYFNENDCWVQVEGNRGRVGISDYLQQNLSDITFVTPPDPGTEVEQFGEMGTIESAKAVYELVSPVTGRVVAVNEAILEA
PELINENPYEKGWITELELTNFEADREFLLDGRRYMEVLKEKVADFNAGK
>Mature_289_residues
TAKYVILPCNGLDKEAGCLARELALKMAAATGSEIICPVLYQTAPSRYASLLREGSLVVIDGCATRCASRIAANNNLKIY
RKITMTEEAKKRDYNPGPDWRVGAGAAAFVEDVWRSWQPVLREQEAGRAPGENAGLFAGPLEYAIHRHDKFIFRVPLEGF
YFNENDCWVQVEGNRGRVGISDYLQQNLSDITFVTPPDPGTEVEQFGEMGTIESAKAVYELVSPVTGRVVAVNEAILEAP
ELINENPYEKGWITELELTNFEADREFLLDGRRYMEVLKEKVADFNAGK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=99, Percent_Identity=41.4141414141414, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI89057342, Length=99, Percent_Identity=40.4040404040404, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=35.6589147286822, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507493, Length=130, Percent_Identity=33.8461538461538, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=33.0508474576271, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319272, Length=92, Percent_Identity=35.8695652173913, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17865652, Length=101, Percent_Identity=43.5643564356436, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 32276; Mature: 32145

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKYVILPCNGLDKEAGCLARELALKMAAATGSEIICPVLYQTAPSRYASLLREGSLVV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHHHCCCEEE
IDGCATRCASRIAANNNLKIYRKITMTEEAKKRDYNPGPDWRVGAGAAAFVEDVWRSWQP
EECHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VLREQEAGRAPGENAGLFAGPLEYAIHRHDKFIFRVPLEGFYFNENDCWVQVEGNRGRVG
HHHHHHCCCCCCCCCCEEECHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEECCCCCCC
ISDYLQQNLSDITFVTPPDPGTEVEQFGEMGTIESAKAVYELVSPVTGRVVAVNEAILEA
HHHHHHCCCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHH
PELINENPYEKGWITELELTNFEADREFLLDGRRYMEVLKEKVADFNAGK
HHHHCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAKYVILPCNGLDKEAGCLARELALKMAAATGSEIICPVLYQTAPSRYASLLREGSLVV
CCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHHHCCCEEE
IDGCATRCASRIAANNNLKIYRKITMTEEAKKRDYNPGPDWRVGAGAAAFVEDVWRSWQP
EECHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VLREQEAGRAPGENAGLFAGPLEYAIHRHDKFIFRVPLEGFYFNENDCWVQVEGNRGRVG
HHHHHHCCCCCCCCCCEEECHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEECCCCCCC
ISDYLQQNLSDITFVTPPDPGTEVEQFGEMGTIESAKAVYELVSPVTGRVVAVNEAILEA
HHHHHHCCCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHH
PELINENPYEKGWITELELTNFEADREFLLDGRRYMEVLKEKVADFNAGK
HHHHCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA