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The map label for this gene is xylF [H]

Identifier: 83590852

GI number: 83590852

Start: 2109667

End: 2110812

Strand: Reverse

Name: xylF [H]

Synonym: Moth_2022

Alternate gene names: 83590852

Gene position: 2110812-2109667 (Counterclockwise)

Preceding gene: 83590853

Following gene: 83590851

Centisome position: 80.3

GC content: 44.15

Gene sequence:

>1146_bases
GTGCTAAAAAAGAAGGCTACCAGGATAATTGCCTTGGCTGCAGTTTTAATCCTGCTGGCTATGACCCTTATAGGTTGCGG
TCAAGGCGGAAATTCGAGCAAAAATGGAGGTAATCAGGGCGCTTCGGCTAAAAATGGCGATAAGATAAAGATAGGTCTCT
CAATGGATGATTTACGCCAGGAACGCTGGCAGCATGATAGAGACTTGTTTGTTGCTAAGGCCAAAGAACTCGGAGCTGAA
GTACTGGTACAATCAGCAAACGGTGATGATGCTACCCAGCTTTCCCAGGCTGAGAACCTCATTTCCCAGGGTATTAATGT
CTTGGTTGTAATACCCCATAATGGAGACGCCATGGCCCCTATTGTCGAAGCAGCGCATAAAGCCGGGGTAAAAGTGCTGG
CATATGATCGTTTAATAAGAAATGCAGATGTAGATCTCTATATTTCCTTTGATAATGTCCGCGTTGGTGAATTGCAGGCA
GAGTACCTGACCAAAAAGGTTCCTAAAGGTAAATATTTCCTTATGGGTGGATCGCCTACAGACAACAATGCAAAATTATT
TAGACAGGGACAGATGAATGTTCTTAAACCACTTATTGATAAGGGAGACATTAAGGTTGTTGGCGATCAGTGGGTTAAAG
ATTGGCTGCCGGAAGAGGCCATGAAGATTATGGAAAATGCCCTGACTAGCAATAATAACCAGATTGATGCTGTAGTCGCA
TCTAATGACAGTACTGCTGGGGGAGCTATTCAAGCTTTAGCTGCCCAGAATCTAGCTGGTAAAGTGGCTATTTCTGGCCA
GGACGCAGATCTTGCTGCCTGCCAGCGTATTGTTGAAGGAACTCAATCTATGACTGTTTATAAACCTATTACGACTCTGG
CTACCCGGGCGGCGGAGGTTGCGGTGGCGCTGGCCAAGGGTGAAAATATTGGGGCTAATAATAAAGTAAATAACGGGAAA
ATAGATGTACCCTCAGTGTTACTGACACCCATTATGGTGGATAAAGATAATATGGTCCAGACGGTAATTAAAGATGGTTT
CCATAAACTTGAAATAAAAACGTCCCCAAAGAAAAGTGGCCTAAGCAATAGGCAATCAATTAATTCAATTAGGCAAGAGG
CCACCGTATGGCCTCTTGCCTGTTAA

Upstream 100 bases:

>100_bases
ATCCGGGGGGTATGGATGAATACTACCTAGCGTACCTCTAATTCTAAGAAAAAGATAAATCTGTCTTTAATTCTAAATGT
GAAAAGGGGGAGCTTTAAGA

Downstream 100 bases:

>100_bases
ATTTAAACAAACGCCGAGTCTATTGGGAAAGAAGGGGTCTATTATGGACCACTATATTCTAGAAATGCAGGAAATTACAA
AGCAATTTCCCGGCGTCAAG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLKKKATRIIALAAVLILLAMTLIGCGQGGNSSKNGGNQGASAKNGDKIKIGLSMDDLRQERWQHDRDLFVAKAKELGAE
VLVQSANGDDATQLSQAENLISQGINVLVVIPHNGDAMAPIVEAAHKAGVKVLAYDRLIRNADVDLYISFDNVRVGELQA
EYLTKKVPKGKYFLMGGSPTDNNAKLFRQGQMNVLKPLIDKGDIKVVGDQWVKDWLPEEAMKIMENALTSNNNQIDAVVA
SNDSTAGGAIQALAAQNLAGKVAISGQDADLAACQRIVEGTQSMTVYKPITTLATRAAEVAVALAKGENIGANNKVNNGK
IDVPSVLLTPIMVDKDNMVQTVIKDGFHKLEIKTSPKKSGLSNRQSINSIRQEATVWPLAC

Sequences:

>Translated_381_residues
MLKKKATRIIALAAVLILLAMTLIGCGQGGNSSKNGGNQGASAKNGDKIKIGLSMDDLRQERWQHDRDLFVAKAKELGAE
VLVQSANGDDATQLSQAENLISQGINVLVVIPHNGDAMAPIVEAAHKAGVKVLAYDRLIRNADVDLYISFDNVRVGELQA
EYLTKKVPKGKYFLMGGSPTDNNAKLFRQGQMNVLKPLIDKGDIKVVGDQWVKDWLPEEAMKIMENALTSNNNQIDAVVA
SNDSTAGGAIQALAAQNLAGKVAISGQDADLAACQRIVEGTQSMTVYKPITTLATRAAEVAVALAKGENIGANNKVNNGK
IDVPSVLLTPIMVDKDNMVQTVIKDGFHKLEIKTSPKKSGLSNRQSINSIRQEATVWPLAC
>Mature_381_residues
MLKKKATRIIALAAVLILLAMTLIGCGQGGNSSKNGGNQGASAKNGDKIKIGLSMDDLRQERWQHDRDLFVAKAKELGAE
VLVQSANGDDATQLSQAENLISQGINVLVVIPHNGDAMAPIVEAAHKAGVKVLAYDRLIRNADVDLYISFDNVRVGELQA
EYLTKKVPKGKYFLMGGSPTDNNAKLFRQGQMNVLKPLIDKGDIKVVGDQWVKDWLPEEAMKIMENALTSNNNQIDAVVA
SNDSTAGGAIQALAAQNLAGKVAISGQDADLAACQRIVEGTQSMTVYKPITTLATRAAEVAVALAKGENIGANNKVNNGK
IDVPSVLLTPIMVDKDNMVQTVIKDGFHKLEIKTSPKKSGLSNRQSINSIRQEATVWPLAC

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=307, Percent_Identity=66.7752442996743, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1790192, Length=277, Percent_Identity=24.9097472924188, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40760; Mature: 40760

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKKATRIIALAAVLILLAMTLIGCGQGGNSSKNGGNQGASAKNGDKIKIGLSMDDLRQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHH
ERWQHDRDLFVAKAKELGAEVLVQSANGDDATQLSQAENLISQGINVLVVIPHNGDAMAP
HHHCCCHHHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
IVEAAHKAGVKVLAYDRLIRNADVDLYISFDNVRVGELQAEYLTKKVPKGKYFLMGGSPT
HHHHHHHCCCEEEEHHHHHHCCCEEEEEEECCEEEHHHHHHHHHHHCCCCCEEEECCCCC
DNNAKLFRQGQMNVLKPLIDKGDIKVVGDQWVKDWLPEEAMKIMENALTSNNNQIDAVVA
CCCHHHHHCCCHHHHHHHHCCCCEEEECHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEE
SNDSTAGGAIQALAAQNLAGKVAISGQDADLAACQRIVEGTQSMTVYKPITTLATRAAEV
CCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
AVALAKGENIGANNKVNNGKIDVPSVLLTPIMVDKDNMVQTVIKDGFHKLEIKTSPKKSG
HEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEECCHHHHHHHHHCCCEEEEEECCCCCCC
LSNRQSINSIRQEATVWPLAC
CCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLKKKATRIIALAAVLILLAMTLIGCGQGGNSSKNGGNQGASAKNGDKIKIGLSMDDLRQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHH
ERWQHDRDLFVAKAKELGAEVLVQSANGDDATQLSQAENLISQGINVLVVIPHNGDAMAP
HHHCCCHHHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
IVEAAHKAGVKVLAYDRLIRNADVDLYISFDNVRVGELQAEYLTKKVPKGKYFLMGGSPT
HHHHHHHCCCEEEEHHHHHHCCCEEEEEEECCEEEHHHHHHHHHHHCCCCCEEEECCCCC
DNNAKLFRQGQMNVLKPLIDKGDIKVVGDQWVKDWLPEEAMKIMENALTSNNNQIDAVVA
CCCHHHHHCCCHHHHHHHHCCCCEEEECHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEE
SNDSTAGGAIQALAAQNLAGKVAISGQDADLAACQRIVEGTQSMTVYKPITTLATRAAEV
CCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
AVALAKGENIGANNKVNNGKIDVPSVLLTPIMVDKDNMVQTVIKDGFHKLEIKTSPKKSG
HEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEECCHHHHHHHHHCCCEEEEEECCCCCCC
LSNRQSINSIRQEATVWPLAC
CCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]