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The map label for this gene is cspR [H]

Identifier: 83590714

GI number: 83590714

Start: 1943298

End: 1943765

Strand: Reverse

Name: cspR [H]

Synonym: Moth_1880

Alternate gene names: 83590714

Gene position: 1943765-1943298 (Counterclockwise)

Preceding gene: 83590715

Following gene: 83590712

Centisome position: 73.94

GC content: 59.62

Gene sequence:

>468_bases
ATGCCCTTGCACGTTGTCCTCTATCAGCCGGAAATACCCCAGAACACCGGCAATATTGCCCGTACCTGCGCCCTAACCGG
GACGTTCCTCCATCTGATTCATCCCCTGGGCTTTCGCCTGGATGAGAAACACCTCAAGCGAGCCGGCCTGGATTACTGGG
AGGAAGTAAATATTCTCGAACACAGCACCTGGGCAGCCTTCCGGGAGGCTTATCCCGATGCCAATTGCTACTACCTGTCC
ACCAAGGGTCAGCGCCGTTATACAAAGGTTGATTACCGACCGGATGATTTCCTCGTCTTCGGTCCCGAGACCCGGGGGCT
GCCGGCCAGTATCCTGGAACCGGCGGGCGACAGGGTGCTCCGGGTGCCCATGCGGCCGGGCCTGAGATCCCTGAACCTGG
CCAACAGCGTGGCTATCGTCCTCTACGAAGCCCTGCGCCAGTTGGATTTTCCCGGCCTCCTTCCGTAG

Upstream 100 bases:

>100_bases
ACCCCGCCAGATTGTACCCTCCAGCCCCCATGAAGCTGGAATATAACTCAACCAGCAATCGCCATTTTTAGTAACTATTA
TTATCGAAGGAGTCAGAGTG

Downstream 100 bases:

>100_bases
CCACATGGTGTTACTGCCAGGCGCGGCAGGCATCGTTTACGCCAGTAATTAGAGGCAGGTCGTGCCTTCTCGATAAAAGC
GCCTTCAAAGTCCTTGTTAA

Product: RNA methyltransferase TrmH, group 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MPLHVVLYQPEIPQNTGNIARTCALTGTFLHLIHPLGFRLDEKHLKRAGLDYWEEVNILEHSTWAAFREAYPDANCYYLS
TKGQRRYTKVDYRPDDFLVFGPETRGLPASILEPAGDRVLRVPMRPGLRSLNLANSVAIVLYEALRQLDFPGLLP

Sequences:

>Translated_155_residues
MPLHVVLYQPEIPQNTGNIARTCALTGTFLHLIHPLGFRLDEKHLKRAGLDYWEEVNILEHSTWAAFREAYPDANCYYLS
TKGQRRYTKVDYRPDDFLVFGPETRGLPASILEPAGDRVLRVPMRPGLRSLNLANSVAIVLYEALRQLDFPGLLP
>Mature_154_residues
PLHVVLYQPEIPQNTGNIARTCALTGTFLHLIHPLGFRLDEKHLKRAGLDYWEEVNILEHSTWAAFREAYPDANCYYLST
KGQRRYTKVDYRPDDFLVFGPETRGLPASILEPAGDRVLRVPMRPGLRSLNLANSVAIVLYEALRQLDFPGLLP

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=53.2894736842105, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17661; Mature: 17529

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHVVLYQPEIPQNTGNIARTCALTGTFLHLIHPLGFRLDEKHLKRAGLDYWEEVNILE
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHCCHHH
HSTWAAFREAYPDANCYYLSTKGQRRYTKVDYRPDDFLVFGPETRGLPASILEPAGDRVL
HHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCEEEECCCCCCCCHHHHCCCCCCEE
RVPMRPGLRSLNLANSVAIVLYEALRQLDFPGLLP
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PLHVVLYQPEIPQNTGNIARTCALTGTFLHLIHPLGFRLDEKHLKRAGLDYWEEVNILE
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHCCHHH
HSTWAAFREAYPDANCYYLSTKGQRRYTKVDYRPDDFLVFGPETRGLPASILEPAGDRVL
HHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCEEEECCCCCCCCHHHHCCCCCCEE
RVPMRPGLRSLNLANSVAIVLYEALRQLDFPGLLP
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]