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The map label for this gene is bioB [H]

Identifier: 83590570

GI number: 83590570

Start: 1786255

End: 1787307

Strand: Reverse

Name: bioB [H]

Synonym: Moth_1735

Alternate gene names: 83590570

Gene position: 1787307-1786255 (Counterclockwise)

Preceding gene: 83590571

Following gene: 83590569

Centisome position: 67.99

GC content: 64.96

Gene sequence:

>1053_bases
GTGCGACCGGAATTTGCTGCAAGCTGGCAGCGGGCGGCAGCAGGTGAAGAACTCGATCGTGAAGATATTGTTAATCTCCT
CGCCGCCACCCCCGGTGAAGAGGAGGAAGCCCTCTACCGGCTGGCGGACGGCGTCCGCGCCCGGATGGCGGGGGACGAGG
TCCACCTGCGCGGTGTCATCGAGTTTTCTAATTACTGTCGTCGTCGCTGTTGCTACTGCGGCCTCCGGGCTGATAACGCC
CGGTTGCACCGCTACCGCCTGATGCCGGAGGATATAGTAGCTGTGGCCAGACAGGGAGTAGAGCTGGGCTATGGTACCAT
CGTCCTCCAGTCCGGGGAGGACCCCTGGTATACGGCCCCGGTCCTGGCAGGGATCGTCCGGGAGATTAAAGAAATGGGGG
TGGCCGTGACCCTGTGTGTAGGCGAGCGCTCCCGGGAGGAGTACGCCCTGTGGCGGGAGGCCGGGGCTGACCGCTACCTC
TTGAAACACGAAACGGCCAACGAAGAACTTTACGCCCGCCTGCACCCCGGCATGAGCTGGCAGGAACGCCTCCAATGCCT
CCAGTGGCTGCGGGAACTGGGCTACCAGGTGGGCTCCGGCAACATCATCGGCCTGCCGGGCCAGACCCTGGCCGACCTGG
CCGACGACTTGCTCCTCCTGCGACAGCTGGATGTGGAGATGGCGGGTTTGGGGCCCTTTATCCCCCACCCGGCAACACCC
CTGGCCGGGGAGCCGGCCGGGAGTTTGGAACTAACTTACCGGGTGGTGGCTACGGCCCGCCTGGTCATTCCTTTCGCCCA
CCTGCCGGCCACCACAGCGGTGGGTACCCTGGCGCCCAACGGCCGCCAGAAGGCCCTGCAGCGGGGGGCCAACGTCATCA
TGCCCAACCTGACCCCCACCCGCTACCGGGCCGACTACCAGATCTATCCCAACAAGATTTGTATCAATGAGGGACCGGAG
GATTGCCGCTACTGCCTGGAGGGCATGGTCCGCGCCTTAGGGCGACGCCTGGGCCGGGGACCGGGGCATACCTTGAAGCC
CATTCCTGCTTAG

Upstream 100 bases:

>100_bases
AGATGGGACGACGGACACGATCGGTGCCCTGACAGGTAAACTGGGAAGCCTGCCCGGGGTGAAGGTCCGGGCGGCCCTGA
CGGGGAAGGAGAGGTAAGCT

Downstream 100 bases:

>100_bases
CCAGTTTGGTTTTATTAAACAAAAGTCCGTAAAACGGAGTGAAAACGGGGAAAAAGCGGAGAGGGTCCATATAAACGTTT
TAAGTGTTCTTAAATAGGAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRPEFAASWQRAAAGEELDREDIVNLLAATPGEEEEALYRLADGVRARMAGDEVHLRGVIEFSNYCRRRCCYCGLRADNA
RLHRYRLMPEDIVAVARQGVELGYGTIVLQSGEDPWYTAPVLAGIVREIKEMGVAVTLCVGERSREEYALWREAGADRYL
LKHETANEELYARLHPGMSWQERLQCLQWLRELGYQVGSGNIIGLPGQTLADLADDLLLLRQLDVEMAGLGPFIPHPATP
LAGEPAGSLELTYRVVATARLVIPFAHLPATTAVGTLAPNGRQKALQRGANVIMPNLTPTRYRADYQIYPNKICINEGPE
DCRYCLEGMVRALGRRLGRGPGHTLKPIPA

Sequences:

>Translated_350_residues
MRPEFAASWQRAAAGEELDREDIVNLLAATPGEEEEALYRLADGVRARMAGDEVHLRGVIEFSNYCRRRCCYCGLRADNA
RLHRYRLMPEDIVAVARQGVELGYGTIVLQSGEDPWYTAPVLAGIVREIKEMGVAVTLCVGERSREEYALWREAGADRYL
LKHETANEELYARLHPGMSWQERLQCLQWLRELGYQVGSGNIIGLPGQTLADLADDLLLLRQLDVEMAGLGPFIPHPATP
LAGEPAGSLELTYRVVATARLVIPFAHLPATTAVGTLAPNGRQKALQRGANVIMPNLTPTRYRADYQIYPNKICINEGPE
DCRYCLEGMVRALGRRLGRGPGHTLKPIPA
>Mature_350_residues
MRPEFAASWQRAAAGEELDREDIVNLLAATPGEEEEALYRLADGVRARMAGDEVHLRGVIEFSNYCRRRCCYCGLRADNA
RLHRYRLMPEDIVAVARQGVELGYGTIVLQSGEDPWYTAPVLAGIVREIKEMGVAVTLCVGERSREEYALWREAGADRYL
LKHETANEELYARLHPGMSWQERLQCLQWLRELGYQVGSGNIIGLPGQTLADLADDLLLLRQLDVEMAGLGPFIPHPATP
LAGEPAGSLELTYRVVATARLVIPFAHLPATTAVGTLAPNGRQKALQRGANVIMPNLTPTRYRADYQIYPNKICINEGPE
DCRYCLEGMVRALGRRLGRGPGHTLKPIPA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=285, Percent_Identity=25.9649122807018, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790423, Length=228, Percent_Identity=28.0701754385965, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38828; Mature: 38828

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPEFAASWQRAAAGEELDREDIVNLLAATPGEEEEALYRLADGVRARMAGDEVHLRGVI
CCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
EFSNYCRRRCCYCGLRADNARLHRYRLMPEDIVAVARQGVELGYGTIVLQSGEDPWYTAP
HHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHCCCEECCEEEEEECCCCCCCHHH
VLAGIVREIKEMGVAVTLCVGERSREEYALWREAGADRYLLKHETANEELYARLHPGMSW
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCH
QERLQCLQWLRELGYQVGSGNIIGLPGQTLADLADDLLLLRQLDVEMAGLGPFIPHPATP
HHHHHHHHHHHHHCCEECCCCEEECCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCC
LAGEPAGSLELTYRVVATARLVIPFAHLPATTAVGTLAPNGRQKALQRGANVIMPNLTPT
CCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCCCCEEECCCCCC
RYRADYQIYPNKICINEGPEDCRYCLEGMVRALGRRLGRGPGHTLKPIPA
CEECCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRPEFAASWQRAAAGEELDREDIVNLLAATPGEEEEALYRLADGVRARMAGDEVHLRGVI
CCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
EFSNYCRRRCCYCGLRADNARLHRYRLMPEDIVAVARQGVELGYGTIVLQSGEDPWYTAP
HHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHCCCEECCEEEEEECCCCCCCHHH
VLAGIVREIKEMGVAVTLCVGERSREEYALWREAGADRYLLKHETANEELYARLHPGMSW
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCH
QERLQCLQWLRELGYQVGSGNIIGLPGQTLADLADDLLLLRQLDVEMAGLGPFIPHPATP
HHHHHHHHHHHHHCCEECCCCEEECCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCC
LAGEPAGSLELTYRVVATARLVIPFAHLPATTAVGTLAPNGRQKALQRGANVIMPNLTPT
CCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCCCCEEECCCCCC
RYRADYQIYPNKICINEGPEDCRYCLEGMVRALGRRLGRGPGHTLKPIPA
CEECCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA