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The map label for this gene is thiH

Identifier: 83590569

GI number: 83590569

Start: 1784737

End: 1786140

Strand: Reverse

Name: thiH

Synonym: Moth_1734

Alternate gene names: 83590569

Gene position: 1786140-1784737 (Counterclockwise)

Preceding gene: 83590570

Following gene: 83590568

Centisome position: 67.95

GC content: 60.68

Gene sequence:

>1404_bases
ATGCAACACGGTTATCGCGCCGATTTTATCAATCATGAAGAGATAGAAGGCTACCTGGAAGAAGCTAAACGGGCAACGAG
GGATGTGGCTGTCAGGATTATCGAAAAGGCGCGGGAAGCGAAGGGGCTGGAGCCCTACGAGGTGGCGGTTTTGCTCCAGA
ACGACGATGCGGACGTACGCCGGCGGATGTTTACCGCCGCCCGGGAGATAAAGGAAAAGATCTACGGCCAGCGGATAGTT
CTTTTTGCACCTCTGTACTTCAGCGACTACTGCATTAACAACTGCCGCTACTGCGGCTACCGGCGGGAAAATAAGTTCGA
ACGCCGCCGCCTGGAGCCGGAGGAACTGGAACGGGAGGTGCGCATCCTGGAATCCCTGGGGCATAAGCGCCTGGCCCTGG
AGGCCGGGGAGGATCCCGTCCATTGTCCCCTTGAATATACCCTGGATGTTATTAACCGCATTTACCGCATCACCGAAGCC
AACGGCAGCATCCGGCGGGTAAACGTCAACATCGCGGCGACGACGGTGGATGCCTACAGGCAGTTAAAGGCCGCCGGCAT
CGGCACCTACGTCCTCTTCCAGGAGACCTACCACCGGCCTACTTATGCCTACATGCACCCCGGCGGCCCCAAGGCGGACT
ACGACTGGCACACCACGGCCATGGACCGGGCCATGGAGGGCGGCATCGACGACGTCGGCCTGGGGGTCCTCTTCGGCCTC
TACGATTATAAATTCGAAGTCATGGGCCTGCTCTACCATGCCCGGCACTTGGAGGAGACCTTCGGCGTCGGCCCCCATAC
CATCTCCGTACCGCGCCTGCGGCCGGCCTACAACATTACCCTGGAAAAATTCCCTTACCTGGTTGACGACGAAGATTTTA
AGAAACTGGTGGCCATCATCCGCCTGGCCGTGCCCTATACCGGCATGATCATCTCCACCCGGGAGACGGCGGAGCTCAGG
GCGGAACTCCTGGAGTTGGGCGTTTCCCAGATCAGCGCCGGCTCCTGTACGGGGGTAGGGGGCTATGGCCGTCACTATGC
CGATCAGGAAGACGATATCCCCCAGTTTGAAATCGGCGACCACCGCCACCCCGATGAGGTTATCGGCGACCTCTGCCGGC
GGGGGTATCTCCCCAGCTACTGCACAGCCTGCTACCGCCGCGGCCGCACCGGCGACCGCTTCATGTCCCTGGCCAAAACC
GGGGAGATCCAGCACTGCTGCCAGCCTAACGCCATCCTCACCTTTAAGGAATACTTGCTGGATTATGCCCGCCCGGCTAC
CAGGGAAGTAGGAGAGACAACCATCAGGGAGCACCTGGCCCGGATCCCCAGCCCGGCCATCCGGGCCGAAACGGAACGCC
GCCTGGAGCGCATCGCCGCCGGCGAGCGGGATTTGTATTTCTAG

Upstream 100 bases:

>100_bases
TTAAACAAAAGTCCGTAAAACGGAGTGAAAACGGGGAAAAAGCGGAGAGGGTCCATATAAACGTTTTAAGTGTTCTTAAA
TAGGAATGGAGGAATTTTCC

Downstream 100 bases:

>100_bases
TGCCGCTACCCCCTAACGGCGCTTTAACCCCCCGTCCTTCAGGGCAGGGGGTTTTATCATGGGTGGAGATAAACGGGGGG
CCAGCGTTTGGGTGCCGGTT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MQHGYRADFINHEEIEGYLEEAKRATRDVAVRIIEKAREAKGLEPYEVAVLLQNDDADVRRRMFTAAREIKEKIYGQRIV
LFAPLYFSDYCINNCRYCGYRRENKFERRRLEPEELEREVRILESLGHKRLALEAGEDPVHCPLEYTLDVINRIYRITEA
NGSIRRVNVNIAATTVDAYRQLKAAGIGTYVLFQETYHRPTYAYMHPGGPKADYDWHTTAMDRAMEGGIDDVGLGVLFGL
YDYKFEVMGLLYHARHLEETFGVGPHTISVPRLRPAYNITLEKFPYLVDDEDFKKLVAIIRLAVPYTGMIISTRETAELR
AELLELGVSQISAGSCTGVGGYGRHYADQEDDIPQFEIGDHRHPDEVIGDLCRRGYLPSYCTACYRRGRTGDRFMSLAKT
GEIQHCCQPNAILTFKEYLLDYARPATREVGETTIREHLARIPSPAIRAETERRLERIAAGERDLYF

Sequences:

>Translated_467_residues
MQHGYRADFINHEEIEGYLEEAKRATRDVAVRIIEKAREAKGLEPYEVAVLLQNDDADVRRRMFTAAREIKEKIYGQRIV
LFAPLYFSDYCINNCRYCGYRRENKFERRRLEPEELEREVRILESLGHKRLALEAGEDPVHCPLEYTLDVINRIYRITEA
NGSIRRVNVNIAATTVDAYRQLKAAGIGTYVLFQETYHRPTYAYMHPGGPKADYDWHTTAMDRAMEGGIDDVGLGVLFGL
YDYKFEVMGLLYHARHLEETFGVGPHTISVPRLRPAYNITLEKFPYLVDDEDFKKLVAIIRLAVPYTGMIISTRETAELR
AELLELGVSQISAGSCTGVGGYGRHYADQEDDIPQFEIGDHRHPDEVIGDLCRRGYLPSYCTACYRRGRTGDRFMSLAKT
GEIQHCCQPNAILTFKEYLLDYARPATREVGETTIREHLARIPSPAIRAETERRLERIAAGERDLYF
>Mature_467_residues
MQHGYRADFINHEEIEGYLEEAKRATRDVAVRIIEKAREAKGLEPYEVAVLLQNDDADVRRRMFTAAREIKEKIYGQRIV
LFAPLYFSDYCINNCRYCGYRRENKFERRRLEPEELEREVRILESLGHKRLALEAGEDPVHCPLEYTLDVINRIYRITEA
NGSIRRVNVNIAATTVDAYRQLKAAGIGTYVLFQETYHRPTYAYMHPGGPKADYDWHTTAMDRAMEGGIDDVGLGVLFGL
YDYKFEVMGLLYHARHLEETFGVGPHTISVPRLRPAYNITLEKFPYLVDDEDFKKLVAIIRLAVPYTGMIISTRETAELR
AELLELGVSQISAGSCTGVGGYGRHYADQEDDIPQFEIGDHRHPDEVIGDLCRRGYLPSYCTACYRRGRTGDRFMSLAKT
GEIQHCCQPNAILTFKEYLLDYARPATREVGETTIREHLARIPSPAIRAETERRLERIAAGERDLYF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=375, Percent_Identity=28, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53671; Mature: 53671

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHGYRADFINHEEIEGYLEEAKRATRDVAVRIIEKAREAKGLEPYEVAVLLQNDDADVR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHH
RRMFTAAREIKEKIYGQRIVLFAPLYFSDYCINNCRYCGYRRENKFERRRLEPEELEREV
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHH
RILESLGHKRLALEAGEDPVHCPLEYTLDVINRIYRITEANGSIRRVNVNIAATTVDAYR
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHH
QLKAAGIGTYVLFQETYHRPTYAYMHPGGPKADYDWHTTAMDRAMEGGIDDVGLGVLFGL
HHHHCCCCEEEEEHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
YDYKFEVMGLLYHARHLEETFGVGPHTISVPRLRPAYNITLEKFPYLVDDEDFKKLVAII
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEHHHCCCCCCCHHHHHHHHHH
RLAVPYTGMIISTRETAELRAELLELGVSQISAGSCTGVGGYGRHYADQEDDIPQFEIGD
HHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HRHPDEVIGDLCRRGYLPSYCTACYRRGRTGDRFMSLAKTGEIQHCCQPNAILTFKEYLL
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHH
DYARPATREVGETTIREHLARIPSPAIRAETERRLERIAAGERDLYF
HHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQHGYRADFINHEEIEGYLEEAKRATRDVAVRIIEKAREAKGLEPYEVAVLLQNDDADVR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHH
RRMFTAAREIKEKIYGQRIVLFAPLYFSDYCINNCRYCGYRRENKFERRRLEPEELEREV
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHH
RILESLGHKRLALEAGEDPVHCPLEYTLDVINRIYRITEANGSIRRVNVNIAATTVDAYR
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHH
QLKAAGIGTYVLFQETYHRPTYAYMHPGGPKADYDWHTTAMDRAMEGGIDDVGLGVLFGL
HHHHCCCCEEEEEHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
YDYKFEVMGLLYHARHLEETFGVGPHTISVPRLRPAYNITLEKFPYLVDDEDFKKLVAII
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEHHHCCCCCCCHHHHHHHHHH
RLAVPYTGMIISTRETAELRAELLELGVSQISAGSCTGVGGYGRHYADQEDDIPQFEIGD
HHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HRHPDEVIGDLCRRGYLPSYCTACYRRGRTGDRFMSLAKTGEIQHCCQPNAILTFKEYLL
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHH
DYARPATREVGETTIREHLARIPSPAIRAETERRLERIAAGERDLYF
HHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]