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The map label for this gene is thiC3

Identifier: 83590559

GI number: 83590559

Start: 1767185

End: 1768483

Strand: Reverse

Name: thiC3

Synonym: Moth_1723

Alternate gene names: 83590559

Gene position: 1768483-1767185 (Counterclockwise)

Preceding gene: 83590561

Following gene: 83590558

Centisome position: 67.27

GC content: 58.51

Gene sequence:

>1299_bases
TTGAACCTGATAGAAAGCGCCCGGGCCGGCTTGATTACCCCCGAAATGGAGCAGGTGGCCGTTCAGGAGGGTGTAACTCC
GGAATTCGTGAGGCAGGGTGTGGCTGATGGGACGATAGTCATCCTGCGCAATGCCCGCCGGCAGAACGTCACTCCAGTTG
GTGTCGGTAAAGGGCTGAGGACAAAGGTCAGCGCCAGCGTCGGTTTGTACGGAGAGACGGGCGGTATTGATGTGGAGGTT
GCCAAGATTAAAGCCGCCGTGGAAGCGGGAACGGACGCCATCATGGATCTGAGCGTCAGCGGGGACATCGAGGCCATGCT
TGCGGAAACGCTGGCCGTTTCCCCCAAGCCCGTCGGTACCTTGCCCCTTTACCAGGCCATGGCCGAAGCCGGCAGAAAAT
ACGGTTCTTCCGTTAACATGAGAGATGAAGACTTGTTTGATGTAATTGAACGCCACGCGGCCGCCGGGGTAGACTTTCTG
GCCCTGCACTGCGGGACTACTATGAATATTGTAGAACGCGCCAGAAACGAGGGCCGGATCGATCCTCTGGTAAGCTACGG
GGGTTCCCACCTGATCGGGTGGATGCTGGCGAACCGGAGGGAAAACCCCCTTTATGAACACTTTGACCGGGTTCTTGCGA
TTGCCCGGAAGTACGATGTTACCATCAGCTTTGCCGACGGCATGCGACCGGGATGCCTGGCCGATTCCCTGGATGGCCCC
CAGGTGGAAGAGCTGGTTGTTTTGGGAGAGCTGGTCAGGCGGGCGAGGGAAGCCGGTGTACAGGTGATGGTAAAAGGGCC
GGGTCATGTACCCTTGCAGCAACTAAAGGCGACGGTTGTCCTGGAAAAAAGTCTCTGCCACGGGGCGCCGTATTTTGTCT
TCGGCCCCCTGGTAACAGATATCGCAATCGGTTATGACCATATCAATGCTGCTATCGGGGGTGCCTTGAGCGCCTGGGCG
GGTGCGGAGTTTCTCTGTTATGTAACTGCCGCCGAACATGTGGGGATTCCGGATATTGACCAGGTCCGGGAGGGAGTGAT
TGCCGCTCGCATTGCCGCCCATGCCGCCGACCTGGCCAACGGCCTTACCTGTGCCCGGGAATGGGATCGGGAGCTTTCCC
GGGCGAGAAAAGAACTGGACTGGAAGCGGCAGATTGCTCTCGCCATAGACCCCGAACGGGCGGGAAGGCTGAGAGAAGAA
AGAAGCGACGCCGCGGCGGCGGGATGTGCCATGTGCGGTAAATACTGCGCCATGGAAATCGTATCCAGATACCTGGGCAC
AGCCAGACATACATGTTAG

Upstream 100 bases:

>100_bases
TACCCGGTCATCAAGAAAGCGAAAAAATACTGTGAAGAAGGCAAACATTGCGGCGTAAACCGCTGTTGGAGTTGCAAAAA
TACAGTGGGAGGGAGAATAG

Downstream 100 bases:

>100_bases
GGGGTAGGATAAATGAGCCAGGTTTTAGATGCCCGTGCTGGAAAAATTACTCCGGAAATGGAAAAAGTGGCGGCAGATGA
AAAGGTGGATGTGGAATTTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 3; HMP-P synthase 3; HMP-phosphate synthase 3; HMPP synthase 3; Thiamine biosynthesis protein thiC 3

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNLIESARAGLITPEMEQVAVQEGVTPEFVRQGVADGTIVILRNARRQNVTPVGVGKGLRTKVSASVGLYGETGGIDVEV
AKIKAAVEAGTDAIMDLSVSGDIEAMLAETLAVSPKPVGTLPLYQAMAEAGRKYGSSVNMRDEDLFDVIERHAAAGVDFL
ALHCGTTMNIVERARNEGRIDPLVSYGGSHLIGWMLANRRENPLYEHFDRVLAIARKYDVTISFADGMRPGCLADSLDGP
QVEELVVLGELVRRAREAGVQVMVKGPGHVPLQQLKATVVLEKSLCHGAPYFVFGPLVTDIAIGYDHINAAIGGALSAWA
GAEFLCYVTAAEHVGIPDIDQVREGVIAARIAAHAADLANGLTCAREWDRELSRARKELDWKRQIALAIDPERAGRLREE
RSDAAAAGCAMCGKYCAMEIVSRYLGTARHTC

Sequences:

>Translated_432_residues
MNLIESARAGLITPEMEQVAVQEGVTPEFVRQGVADGTIVILRNARRQNVTPVGVGKGLRTKVSASVGLYGETGGIDVEV
AKIKAAVEAGTDAIMDLSVSGDIEAMLAETLAVSPKPVGTLPLYQAMAEAGRKYGSSVNMRDEDLFDVIERHAAAGVDFL
ALHCGTTMNIVERARNEGRIDPLVSYGGSHLIGWMLANRRENPLYEHFDRVLAIARKYDVTISFADGMRPGCLADSLDGP
QVEELVVLGELVRRAREAGVQVMVKGPGHVPLQQLKATVVLEKSLCHGAPYFVFGPLVTDIAIGYDHINAAIGGALSAWA
GAEFLCYVTAAEHVGIPDIDQVREGVIAARIAAHAADLANGLTCAREWDRELSRARKELDWKRQIALAIDPERAGRLREE
RSDAAAAGCAMCGKYCAMEIVSRYLGTARHTC
>Mature_432_residues
MNLIESARAGLITPEMEQVAVQEGVTPEFVRQGVADGTIVILRNARRQNVTPVGVGKGLRTKVSASVGLYGETGGIDVEV
AKIKAAVEAGTDAIMDLSVSGDIEAMLAETLAVSPKPVGTLPLYQAMAEAGRKYGSSVNMRDEDLFDVIERHAAAGVDFL
ALHCGTTMNIVERARNEGRIDPLVSYGGSHLIGWMLANRRENPLYEHFDRVLAIARKYDVTISFADGMRPGCLADSLDGP
QVEELVVLGELVRRAREAGVQVMVKGPGHVPLQQLKATVVLEKSLCHGAPYFVFGPLVTDIAIGYDHINAAIGGALSAWA
GAEFLCYVTAAEHVGIPDIDQVREGVIAARIAAHAADLANGLTCAREWDRELSRARKELDWKRQIALAIDPERAGRLREE
RSDAAAAGCAMCGKYCAMEIVSRYLGTARHTC

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=449, Percent_Identity=38.3073496659243, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC3_MOOTA (Q2RHR4)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430568.1
- STRING:   Q2RHR4
- GeneID:   3833023
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1723
- NMPDR:   fig|264732.9.peg.1725
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- ProtClustDB:   PRK13352
- BioCyc:   MTHE264732:MOTH_1723-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 46399; Mature: 46399

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIESARAGLITPEMEQVAVQEGVTPEFVRQGVADGTIVILRNARRQNVTPVGVGKGLR
CCCHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCC
TKVSASVGLYGETGGIDVEVAKIKAAVEAGTDAIMDLSVSGDIEAMLAETLAVSPKPVGT
HHHHCCEEECCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCC
LPLYQAMAEAGRKYGSSVNMRDEDLFDVIERHAAAGVDFLALHCGTTMNIVERARNEGRI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCC
DPLVSYGGSHLIGWMLANRRENPLYEHFDRVLAIARKYDVTISFADGMRPGCLADSLDGP
CHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCC
QVEELVVLGELVRRAREAGVQVMVKGPGHVPLQQLKATVVLEKSLCHGAPYFVFGPLVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAIGYDHINAAIGGALSAWAGAEFLCYVTAAEHVGIPDIDQVREGVIAARIAAHAADLAN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLTCAREWDRELSRARKELDWKRQIALAIDPERAGRLREERSDAAAAGCAMCGKYCAMEI
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSRYLGTARHTC
HHHHHHHHCCCC
>Mature Secondary Structure
MNLIESARAGLITPEMEQVAVQEGVTPEFVRQGVADGTIVILRNARRQNVTPVGVGKGLR
CCCHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCC
TKVSASVGLYGETGGIDVEVAKIKAAVEAGTDAIMDLSVSGDIEAMLAETLAVSPKPVGT
HHHHCCEEECCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCC
LPLYQAMAEAGRKYGSSVNMRDEDLFDVIERHAAAGVDFLALHCGTTMNIVERARNEGRI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCC
DPLVSYGGSHLIGWMLANRRENPLYEHFDRVLAIARKYDVTISFADGMRPGCLADSLDGP
CHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCC
QVEELVVLGELVRRAREAGVQVMVKGPGHVPLQQLKATVVLEKSLCHGAPYFVFGPLVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAIGYDHINAAIGGALSAWAGAEFLCYVTAAEHVGIPDIDQVREGVIAARIAAHAADLAN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLTCAREWDRELSRARKELDWKRQIALAIDPERAGRLREERSDAAAAGCAMCGKYCAMEI
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSRYLGTARHTC
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA