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The map label for this gene is thiC2

Identifier: 83590558

GI number: 83590558

Start: 1765892

End: 1767172

Strand: Reverse

Name: thiC2

Synonym: Moth_1722

Alternate gene names: 83590558

Gene position: 1767172-1765892 (Counterclockwise)

Preceding gene: 83590559

Following gene: 83590557

Centisome position: 67.22

GC content: 58.7

Gene sequence:

>1281_bases
ATGAGCCAGGTTTTAGATGCCCGTGCTGGAAAAATTACTCCGGAAATGGAAAAAGTGGCGGCAGATGAAAAGGTGGATGT
GGAATTTGTCCGGGCAGGGGTGGCGGAGGGGACTATTGTAATCCCCCGGAATACCAACCGGAAGGTCCTTAAACCCTGCG
GTATCGGGAGGGGTTTACGTATCAAGGTGAATGCCCTGATCGGGACCTCCAGCGACCGGGATGACCGGCAAATGGAGATG
CGGAAGATCGCTGCGGCCGAGGCGGCAGGGTGTGATTCCTTTATGGATTTAAGCACCGGCGGGGATATCGATGAGATGCG
GCGGCTCACCCTTGCCCACGCCAGGGTTCCGGTAGGCAGCGTGCCCATTTATCAGGCAGCCATCGAAGCCATTGAAAAGC
GGGGCAGTATTGTAGCGATGACGCCGGACGACATGTTTGCGGCCGTGGAAAAACAGGCAAGGGACGGGATAGATTTCATG
GCCATTCACAGCGCCCTGAATTTCGAGATCCTCGAAAGGCTTCAGGCTAGCGGCAGGGTGACCGACATTGTCAGCCGCGG
TGGGGCCTTCCTCACCGGCTGGATGCTGCACAACCAGAAAGAGAATCCCCTTTATGAGCAGTTCGACAGGTTGCTCGAAA
TCTTGCTGAAGTACGATGTCACCCTCAGCCTCGGCGACGCCATTCGTCCGGGTTCTACAGCCGACTCCCTGGACGGGGCC
CAACTGCAGGGAATGATCGTGGCCGGGGAACTGGTCAGGCGCGCCAGGGAAGCCGGCGTGCAGGTTATGGTCGAGGGTCC
GGGACATGTTCCCCTCAACCATGTGGAAACGACAATGAAACTACAGAAAAGCCTGTGCGGGGGCGCGCCTTACTTTATTC
TGGGTACCCTGGCTACTGATGTGGCGCCGGGATATGACCATATCACTGCCGCAATAGGGGGTGCCCTTGCCGGGACGGTT
GGGGCGGATTTTATCTGCTATGTGACACCGGCGGAGCATCTGGGGTTACCAACAGAGCAGGACGTTAAAGAAGGGGTGAT
TGCCGCCCGCATTGCCGCCCATGCCGCCGATCTGGCCAGGGGAAACAGGCAGGCCTGGGAGCGGGATCTGCAAATGGCGC
GGGCGCGGGTCGCCCTCGATGTGGAAAAGCAGATAAGCCTTGCCATTGATCAGGAAAAGGCACGCTCGTTGCTCGACGGT
ACCGGGGAAGACGGGGTTTGTGCTGCCTGTGGGACGAACTGCGCAGCCCTGGTGGCCGCCCGTTATTTCGGGATGAACTG
A

Upstream 100 bases:

>100_bases
CGCGGCGGCGGGATGTGCCATGTGCGGTAAATACTGCGCCATGGAAATCGTATCCAGATACCTGGGCACAGCCAGACATA
CATGTTAGGGGGTAGGATAA

Downstream 100 bases:

>100_bases
GGCGTGAAAACCAGGGGGGAAAATGATGGGATTAAAGCTGGAGGAGACGGTTAAAGGGATCATGCCTGTCAACGATGCCT
GGCGCCGGAAGGCCCGTGAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 2; HMP-P synthase 2; HMP-phosphate synthase 2; HMPP synthase 2; Thiamine biosynthesis protein thiC 2

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSQVLDARAGKITPEMEKVAADEKVDVEFVRAGVAEGTIVIPRNTNRKVLKPCGIGRGLRIKVNALIGTSSDRDDRQMEM
RKIAAAEAAGCDSFMDLSTGGDIDEMRRLTLAHARVPVGSVPIYQAAIEAIEKRGSIVAMTPDDMFAAVEKQARDGIDFM
AIHSALNFEILERLQASGRVTDIVSRGGAFLTGWMLHNQKENPLYEQFDRLLEILLKYDVTLSLGDAIRPGSTADSLDGA
QLQGMIVAGELVRRAREAGVQVMVEGPGHVPLNHVETTMKLQKSLCGGAPYFILGTLATDVAPGYDHITAAIGGALAGTV
GADFICYVTPAEHLGLPTEQDVKEGVIAARIAAHAADLARGNRQAWERDLQMARARVALDVEKQISLAIDQEKARSLLDG
TGEDGVCAACGTNCAALVAARYFGMN

Sequences:

>Translated_426_residues
MSQVLDARAGKITPEMEKVAADEKVDVEFVRAGVAEGTIVIPRNTNRKVLKPCGIGRGLRIKVNALIGTSSDRDDRQMEM
RKIAAAEAAGCDSFMDLSTGGDIDEMRRLTLAHARVPVGSVPIYQAAIEAIEKRGSIVAMTPDDMFAAVEKQARDGIDFM
AIHSALNFEILERLQASGRVTDIVSRGGAFLTGWMLHNQKENPLYEQFDRLLEILLKYDVTLSLGDAIRPGSTADSLDGA
QLQGMIVAGELVRRAREAGVQVMVEGPGHVPLNHVETTMKLQKSLCGGAPYFILGTLATDVAPGYDHITAAIGGALAGTV
GADFICYVTPAEHLGLPTEQDVKEGVIAARIAAHAADLARGNRQAWERDLQMARARVALDVEKQISLAIDQEKARSLLDG
TGEDGVCAACGTNCAALVAARYFGMN
>Mature_425_residues
SQVLDARAGKITPEMEKVAADEKVDVEFVRAGVAEGTIVIPRNTNRKVLKPCGIGRGLRIKVNALIGTSSDRDDRQMEMR
KIAAAEAAGCDSFMDLSTGGDIDEMRRLTLAHARVPVGSVPIYQAAIEAIEKRGSIVAMTPDDMFAAVEKQARDGIDFMA
IHSALNFEILERLQASGRVTDIVSRGGAFLTGWMLHNQKENPLYEQFDRLLEILLKYDVTLSLGDAIRPGSTADSLDGAQ
LQGMIVAGELVRRAREAGVQVMVEGPGHVPLNHVETTMKLQKSLCGGAPYFILGTLATDVAPGYDHITAAIGGALAGTVG
ADFICYVTPAEHLGLPTEQDVKEGVIAARIAAHAADLARGNRQAWERDLQMARARVALDVEKQISLAIDQEKARSLLDGT
GEDGVCAACGTNCAALVAARYFGMN

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=451, Percent_Identity=40.5764966740577, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC2_MOOTA (Q2RHR5)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430567.1
- STRING:   Q2RHR5
- GeneID:   3833022
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1722
- NMPDR:   fig|264732.9.peg.1724
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   RARCETH
- ProtClustDB:   PRK13352
- BioCyc:   MTHE264732:MOTH_1722-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 45581; Mature: 45450

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVLDARAGKITPEMEKVAADEKVDVEFVRAGVAEGTIVIPRNTNRKVLKPCGIGRGLR
CCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCE
IKVNALIGTSSDRDDRQMEMRKIAAAEAAGCDSFMDLSTGGDIDEMRRLTLAHARVPVGS
EEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCC
VPIYQAAIEAIEKRGSIVAMTPDDMFAAVEKQARDGIDFMAIHSALNFEILERLQASGRV
CHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
TDIVSRGGAFLTGWMLHNQKENPLYEQFDRLLEILLKYDVTLSLGDAIRPGSTADSLDGA
HHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCC
QLQGMIVAGELVRRAREAGVQVMVEGPGHVPLNHVETTMKLQKSLCGGAPYFILGTLATD
HHHHEEHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VAPGYDHITAAIGGALAGTVGADFICYVTPAEHLGLPTEQDVKEGVIAARIAAHAADLAR
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GNRQAWERDLQMARARVALDVEKQISLAIDQEKARSLLDGTGEDGVCAACGTNCAALVAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCCCCEEEECCCCHHHHHHH
RYFGMN
HHHCCC
>Mature Secondary Structure 
SQVLDARAGKITPEMEKVAADEKVDVEFVRAGVAEGTIVIPRNTNRKVLKPCGIGRGLR
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCE
IKVNALIGTSSDRDDRQMEMRKIAAAEAAGCDSFMDLSTGGDIDEMRRLTLAHARVPVGS
EEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCC
VPIYQAAIEAIEKRGSIVAMTPDDMFAAVEKQARDGIDFMAIHSALNFEILERLQASGRV
CHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
TDIVSRGGAFLTGWMLHNQKENPLYEQFDRLLEILLKYDVTLSLGDAIRPGSTADSLDGA
HHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCC
QLQGMIVAGELVRRAREAGVQVMVEGPGHVPLNHVETTMKLQKSLCGGAPYFILGTLATD
HHHHEEHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VAPGYDHITAAIGGALAGTVGADFICYVTPAEHLGLPTEQDVKEGVIAARIAAHAADLAR
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GNRQAWERDLQMARARVALDVEKQISLAIDQEKARSLLDGTGEDGVCAACGTNCAALVAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCCCCEEEECCCCHHHHHHH
RYFGMN
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA