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The map label for this gene is dacB [H]

Identifier: 83590204

GI number: 83590204

Start: 1401602

End: 1402759

Strand: Reverse

Name: dacB [H]

Synonym: Moth_1357

Alternate gene names: 83590204

Gene position: 1402759-1401602 (Counterclockwise)

Preceding gene: 83590205

Following gene: 83590203

Centisome position: 53.36

GC content: 56.82

Gene sequence:

>1158_bases
TTGTTCAAGAAGTATGGCCCCCGGTTCCTTGCTGCCGGGGTCTTTTTATTTATTTTCCTTAGCTTTATTAAACCAGGCCG
GGCTGCTGCCCCGGACATTCAGGCCGAGAGTTATGTATTAATGGATTTTCGGACGGGCCAGGTCCTTATGGCCAAGAATC
CCCATGAGCGGAGGCCCCAGGCCATTACCACCAAGATTACCACGGCCATTTTAGCCCTGGAGCGCGGCAACTTGAACGAC
CAGGTTATTGCCAGTAAAAATGCCGCCGAGACGCCGGAGAGTTCTATTTATCTCCAGGAAGGGGAGACCCTGACCCTGGA
GGAGCTTCTCTACGCCCTGCTACTGCGCTCGGCCAATGATGCCGCCGTGGCCATTGCTGAGCATATCGGTGGCAGTGTTG
AAAACTTTGCCCGCATGATGAATGCGAAGGTCCAGGAGATTGGGGCCCGGGATACCCACTATGTTAACCCCCACGGTCTG
ACAGCTCCGGACCACTACTCCTCGGCTTACGATCTGGCCCTTATTGGCCGTTACGCCATGATGAATCCTAAATTTCGGGA
GATTGTCGCTACCCGCCAGCGGATTATCCCCTGGGCCGGCAAACCCTGGCCGCGATTGCTGATCAATGAAAATCGCCTGC
TATGGGGCTATTATGCTTACCCCGGTGCCGATGGGGTAAAGAACGGCTATACTACCCCGGCCGGGCAGGTCCTGGTGGCC
TCGGCCACCAGGGATAACTGGCGGCTCATCGCCGTGGTCATGAAGTCCCCCAATATGTACCGGGAAACCAGTGCCATCCT
CGATTACGGGTTTAATAACTTTCACCAGGTGAAGTTAATGCCTGCCGGCCAGCAGGTAGCCCTGGCCGGTGTCCGCGGCG
GCATAGCAGCAAATATACCTGCCGTCACGGCCGATGACGTCCTGGTGGTCGAGCCAAAGAATGAGACCTGGACCTGGCAG
CAGCGGGTGGAACTTAATCCCGACCTCAATGCGCCGGTCAAGAAGGGGGATAGGATCGGGCGGATAATCTTCACTTCCCA
CGACCAGGAAGTAAGTGTGGACCTGATAGCTGCCGGTGACGTAGCCCCCCGGCCCTGGTGGATGGGCTTCCTGGAAGCCT
TCCTAACAGTCTTCAACCTGCCCTGGTTGCGGTTCTAG

Upstream 100 bases:

>100_bases
GAAGAATAAGTAAACATAAAATTTCTATCCCTGGCAGGGAAAAGGCCCTGCCAGGGCGAATATAAACCTCCCGGAGTAAG
TTTTACAGGGGGTTTCACTC

Downstream 100 bases:

>100_bases
AAAGGCAAGTCCCTTCATAAATCTTACTAACCGAGGTAATTTATGGAGGGATTGCCGGTGGTCAATCTCATCTGGTTGTT
AATGCTCCTGGCAGGTATAG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MFKKYGPRFLAAGVFLFIFLSFIKPGRAAAPDIQAESYVLMDFRTGQVLMAKNPHERRPQAITTKITTAILALERGNLND
QVIASKNAAETPESSIYLQEGETLTLEELLYALLLRSANDAAVAIAEHIGGSVENFARMMNAKVQEIGARDTHYVNPHGL
TAPDHYSSAYDLALIGRYAMMNPKFREIVATRQRIIPWAGKPWPRLLINENRLLWGYYAYPGADGVKNGYTTPAGQVLVA
SATRDNWRLIAVVMKSPNMYRETSAILDYGFNNFHQVKLMPAGQQVALAGVRGGIAANIPAVTADDVLVVEPKNETWTWQ
QRVELNPDLNAPVKKGDRIGRIIFTSHDQEVSVDLIAAGDVAPRPWWMGFLEAFLTVFNLPWLRF

Sequences:

>Translated_385_residues
MFKKYGPRFLAAGVFLFIFLSFIKPGRAAAPDIQAESYVLMDFRTGQVLMAKNPHERRPQAITTKITTAILALERGNLND
QVIASKNAAETPESSIYLQEGETLTLEELLYALLLRSANDAAVAIAEHIGGSVENFARMMNAKVQEIGARDTHYVNPHGL
TAPDHYSSAYDLALIGRYAMMNPKFREIVATRQRIIPWAGKPWPRLLINENRLLWGYYAYPGADGVKNGYTTPAGQVLVA
SATRDNWRLIAVVMKSPNMYRETSAILDYGFNNFHQVKLMPAGQQVALAGVRGGIAANIPAVTADDVLVVEPKNETWTWQ
QRVELNPDLNAPVKKGDRIGRIIFTSHDQEVSVDLIAAGDVAPRPWWMGFLEAFLTVFNLPWLRF
>Mature_385_residues
MFKKYGPRFLAAGVFLFIFLSFIKPGRAAAPDIQAESYVLMDFRTGQVLMAKNPHERRPQAITTKITTAILALERGNLND
QVIASKNAAETPESSIYLQEGETLTLEELLYALLLRSANDAAVAIAEHIGGSVENFARMMNAKVQEIGARDTHYVNPHGL
TAPDHYSSAYDLALIGRYAMMNPKFREIVATRQRIIPWAGKPWPRLLINENRLLWGYYAYPGADGVKNGYTTPAGQVLVA
SATRDNWRLIAVVMKSPNMYRETSAILDYGFNNFHQVKLMPAGQQVALAGVRGGIAANIPAVTADDVLVVEPKNETWTWQ
QRVELNPDLNAPVKKGDRIGRIIFTSHDQEVSVDLIAAGDVAPRPWWMGFLEAFLTVFNLPWLRF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=364, Percent_Identity=32.1428571428571, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787062, Length=362, Percent_Identity=30.3867403314917, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786851, Length=337, Percent_Identity=31.4540059347181, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082054, Length=228, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42902; Mature: 42902

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKYGPRFLAAGVFLFIFLSFIKPGRAAAPDIQAESYVLMDFRTGQVLMAKNPHERRPQ
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCC
AITTKITTAILALERGNLNDQVIASKNAAETPESSIYLQEGETLTLEELLYALLLRSAND
HHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCC
AAVAIAEHIGGSVENFARMMNAKVQEIGARDTHYVNPHGLTAPDHYSSAYDLALIGRYAM
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
MNPKFREIVATRQRIIPWAGKPWPRLLINENRLLWGYYAYPGADGVKNGYTTPAGQVLVA
CCCHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCEEEE
SATRDNWRLIAVVMKSPNMYRETSAILDYGFNNFHQVKLMPAGQQVALAGVRGGIAANIP
ECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCC
AVTADDVLVVEPKNETWTWQQRVELNPDLNAPVKKGDRIGRIIFTSHDQEVSVDLIAAGD
CCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEECC
VAPRPWWMGFLEAFLTVFNLPWLRF
CCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFKKYGPRFLAAGVFLFIFLSFIKPGRAAAPDIQAESYVLMDFRTGQVLMAKNPHERRPQ
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCC
AITTKITTAILALERGNLNDQVIASKNAAETPESSIYLQEGETLTLEELLYALLLRSAND
HHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCC
AAVAIAEHIGGSVENFARMMNAKVQEIGARDTHYVNPHGLTAPDHYSSAYDLALIGRYAM
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
MNPKFREIVATRQRIIPWAGKPWPRLLINENRLLWGYYAYPGADGVKNGYTTPAGQVLVA
CCCHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCEEEE
SATRDNWRLIAVVMKSPNMYRETSAILDYGFNNFHQVKLMPAGQQVALAGVRGGIAANIP
ECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCC
AVTADDVLVVEPKNETWTWQQRVELNPDLNAPVKKGDRIGRIIFTSHDQEVSVDLIAAGD
CCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEECC
VAPRPWWMGFLEAFLTVFNLPWLRF
CCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]