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The map label for this gene is ddl

Identifier: 83589988

GI number: 83589988

Start: 1170723

End: 1171652

Strand: Reverse

Name: ddl

Synonym: Moth_1140

Alternate gene names: 83589988

Gene position: 1171652-1170723 (Counterclockwise)

Preceding gene: 83589989

Following gene: 83589987

Centisome position: 44.57

GC content: 58.17

Gene sequence:

>930_bases
ATGAAAATTGCCGTCCTAATGGGTGGCCCCTCTTCCGAAAGGGAGATTTCTTTAAAAAGCGGGTCTGCCGTTGCCGCCGC
CCTGTCCGGCCTGGGTCACCAGGTGATAACTATAGATCTGAACAGGGAGGTAGTAGCCAGGTTAAAAAACTTCGCCCCCG
ACGTTGTCTTTAACGCCCTCCACGGTAAACCCGGGGAAGACGGTTCTGTCCAGGGCCTGCTGGAGGTCCTGGGCCTGCCT
TATACTGGCAGCCGCGTCCTGGCCAGTGCCATAACAATGGATAAAATTATGACTAAACGCGTCCTGCTCCAGGCCGGTAT
CCCCACCCCCAAATTTTTAGCCTGGACCGGTGCTGAATACGCCACCGGCAAGAAAGAGATAAAGGCGGCGATATTAAAGG
AACTAGGTTTGCCGGTGGTCATTAAGGCCCCGACCCAGGGTTCCACCATTGGCACCTTTATCGTCAGGGAAGAAGGGGAA
CTGGAGCCGGCCATAGCCGGGGCCCTGAAATATGACCTTTCCTTTATGGCCGAAGCCTACCTGGCAGGCCCGGAGATCAC
GGCTGCCGTCCTGGGGAACCGGAAACCCCAGGTCTTGCCTTTAATAGAAATCGTCTCCCATACCGGCTTTTATGATTATC
AGGCCAAGTACACCCCCGGCCTGAGTGATCATATTATCCCGCCCCGATTGCCGGATGACGTCCTGGCAGCAGCTACCTCC
CTGGCCGGCCGGACCTATGCCCTCCTGGGTTGCCGCGGTTTCGCCCGGGTGGATTTTATCGTGGCGGGGGGCCGGGAGCC
CCAGGTCATTGAAGTCAATAGCGTCCCGGGGATGACCGCCACCAGCCTGGTACCGGACGCCGCCCGGGCGGCAGGATTGG
ATTTTCCGGATCTGGTCCAGAAAATCGTCGACCTGGCCCTGGAGCCTTGA

Upstream 100 bases:

>100_bases
CACTTGGGACCATGGGTATTTCTGCTGCCATGCTGGCCGGGTTAAGTAAGCACCAACCGCCGGGCGCCAATATTTCCCCT
GGGGAGGTCTCATTTTTAAG

Downstream 100 bases:

>100_bases
AACCAACCTGAAAATAGTATATCAGTACCATTTTTATCCTCTGCTAATGCCGCCTGGCTGCATGGACGTCTGATTTGCCC
CGGACCTGGGAATGATGGCG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKIAVLMGGPSSEREISLKSGSAVAAALSGLGHQVITIDLNREVVARLKNFAPDVVFNALHGKPGEDGSVQGLLEVLGLP
YTGSRVLASAITMDKIMTKRVLLQAGIPTPKFLAWTGAEYATGKKEIKAAILKELGLPVVIKAPTQGSTIGTFIVREEGE
LEPAIAGALKYDLSFMAEAYLAGPEITAAVLGNRKPQVLPLIEIVSHTGFYDYQAKYTPGLSDHIIPPRLPDDVLAAATS
LAGRTYALLGCRGFARVDFIVAGGREPQVIEVNSVPGMTATSLVPDAARAAGLDFPDLVQKIVDLALEP

Sequences:

>Translated_309_residues
MKIAVLMGGPSSEREISLKSGSAVAAALSGLGHQVITIDLNREVVARLKNFAPDVVFNALHGKPGEDGSVQGLLEVLGLP
YTGSRVLASAITMDKIMTKRVLLQAGIPTPKFLAWTGAEYATGKKEIKAAILKELGLPVVIKAPTQGSTIGTFIVREEGE
LEPAIAGALKYDLSFMAEAYLAGPEITAAVLGNRKPQVLPLIEIVSHTGFYDYQAKYTPGLSDHIIPPRLPDDVLAAATS
LAGRTYALLGCRGFARVDFIVAGGREPQVIEVNSVPGMTATSLVPDAARAAGLDFPDLVQKIVDLALEP
>Mature_309_residues
MKIAVLMGGPSSEREISLKSGSAVAAALSGLGHQVITIDLNREVVARLKNFAPDVVFNALHGKPGEDGSVQGLLEVLGLP
YTGSRVLASAITMDKIMTKRVLLQAGIPTPKFLAWTGAEYATGKKEIKAAILKELGLPVVIKAPTQGSTIGTFIVREEGE
LEPAIAGALKYDLSFMAEAYLAGPEITAAVLGNRKPQVLPLIEIVSHTGFYDYQAKYTPGLSDHIIPPRLPDDVLAAATS
LAGRTYALLGCRGFARVDFIVAGGREPQVIEVNSVPGMTATSLVPDAARAAGLDFPDLVQKIVDLALEP

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=305, Percent_Identity=41.9672131147541, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=31.1614730878187, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_MOOTA (Q2RJD5)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_429997.1
- ProteinModelPortal:   Q2RJD5
- SMR:   Q2RJD5
- STRING:   Q2RJD5
- GeneID:   3833238
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1140
- NMPDR:   fig|264732.9.peg.1122
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   Q2RJD5
- ProtClustDB:   PRK01372
- BioCyc:   MTHE264732:MOTH_1140-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32548; Mature: 32548

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLMGGPSSEREISLKSGSAVAAALSGLGHQVITIDLNREVVARLKNFAPDVVFNAL
CEEEEEECCCCCCCEEEECCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHCCHHHHHHHH
HGKPGEDGSVQGLLEVLGLPYTGSRVLASAITMDKIMTKRVLLQAGIPTPKFLAWTGAEY
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCH
ATGKKEIKAAILKELGLPVVIKAPTQGSTIGTFIVREEGELEPAIAGALKYDLSFMAEAY
HCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LAGPEITAAVLGNRKPQVLPLIEIVSHTGFYDYQAKYTPGLSDHIIPPRLPDDVLAAATS
HCCCCEEEHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
LAGRTYALLGCRGFARVDFIVAGGREPQVIEVNSVPGMTATSLVPDAARAAGLDFPDLVQ
HCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCCHHHHCCCHHHHCCCCHHHHHH
KIVDLALEP
HHHHHHCCC
>Mature Secondary Structure
MKIAVLMGGPSSEREISLKSGSAVAAALSGLGHQVITIDLNREVVARLKNFAPDVVFNAL
CEEEEEECCCCCCCEEEECCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHCCHHHHHHHH
HGKPGEDGSVQGLLEVLGLPYTGSRVLASAITMDKIMTKRVLLQAGIPTPKFLAWTGAEY
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCH
ATGKKEIKAAILKELGLPVVIKAPTQGSTIGTFIVREEGELEPAIAGALKYDLSFMAEAY
HCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LAGPEITAAVLGNRKPQVLPLIEIVSHTGFYDYQAKYTPGLSDHIIPPRLPDDVLAAATS
HCCCCEEEHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
LAGRTYALLGCRGFARVDFIVAGGREPQVIEVNSVPGMTATSLVPDAARAAGLDFPDLVQ
HCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCCHHHHCCCHHHHCCCCHHHHHH
KIVDLALEP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA