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The map label for this gene is nrgA [H]

Identifier: 83589061

GI number: 83589061

Start: 188313

End: 189716

Strand: Reverse

Name: nrgA [H]

Synonym: Moth_0192

Alternate gene names: 83589061

Gene position: 189716-188313 (Counterclockwise)

Preceding gene: 83589064

Following gene: 83589060

Centisome position: 7.22

GC content: 65.88

Gene sequence:

>1404_bases
TTGTTCCTGGCTGCCGGGACCGTCCTGGCCCGGCCGGCGCTGGCGGCAGCCGGGGACGCGGTTGATACCGGCGATACCGC
TTTTATCTTAGTAGCCAGCGCCCTGGTCATGTTGATGACCCCCGGCCTGGCCTTCTTTTACGGCGGTATGGTCCGCCAGA
AGAACGTCCTGGGTATCATCATGCAATGCCTGGCCACTCTGGCCCTTATTTCCGTCCAGTGGGTCCTGTGGGGCTATTCC
CTGGCCTTTGGTCCCGACCATCTCCACCTGCTGGGAGGGCTGGACTGGGCGGGGTTCCGGGGCGTCGGTCTGGAACCCAA
TCCCCTTTACGCCGCCACCATCCCCCACCAGGTATTCGCCATGTTCCAGCTCATGTTCGCAATAATCACCCCGGCCCTGA
TAACCGGTGCCTTTGCCGAGCGAATGCGCTTCCCGGCCTTCCTGCTCTTTACCTTAATGTGGGCTACCCTGGTCTATGAC
CCCCTGGCCCACTGGGTCTGGGGCGCGGGGGGCTGGCTGCGGGAACTCGGCGCCCTGGACTTTGCCGGCGGCACGGTGGT
GCATATCAGCGCCGGCGTGGCCGGGCTGGTCTGCGCCCTGGTCCTGGGACGCCGGCGGGATTACGGCCGGATCCCCCTGC
CGCCCCACAACCTCCCCATGACCGTTTTGGGCGCCGCGCTGTTATGGTTCGGCTGGTTCGGTTTTAATGCCGGCAGCGCC
CTAGGGGCCAACGGTCTGGCCGGTAACGCCTTCGTCACTACCAATACGGCTGCCGGGGCCGCCATCCTGGGCTGGCTGGC
GGTAGAGTGGCTGCTCCACGGCAAACCCACCGCCCTGGGAGCGGCCAGCGGCGCGGTGGCAGGCCTGGTCGCCATCACCC
CGGCGGCCGGTTTCGTCACTCCCCCGGCGGCCCTGGTAATCGGCGGTATCGGCGGCGCCCTTTGTTGCCTGTCAGTGGCC
GTGGCCAAGGTTAAACTGGGCTACGACGATTCCCTGGACGCCTTTGGCTGCCACGGCGTCGGCGGTACCTGGGGGGCCAT
TGCCACCGGCATCTTTGCCACCAAAGCCGTGAACCCCGCCGGGGCCGACGGCCTGCTTTACGGCAACGCCCATTTGCTGG
GGGTACAGGTGGTAGGCGTGGCGGCGGCCTGGATCTTTACCGCCGCAGTGACCTACGGCATTCTCAAGGTCGTCTCCCTG
TTGACACCTCTGCGGGTCACAGCCGAGGAAGAGGAAGCCGGCCTGGATCTCTCCCAGCACGGCGAAAGCGCCTATCCCGA
CACCGGCCTGGCAGCCCTGGAGGTAGCAGAGGGTAAACGGGTTACCGCCCCGGGAGAACTGGCGGTAAATTGGATGGTGA
GCCAGGGTAACCCGGCCGGAACCGGTCTTTTCCGCCAGGAATAA

Upstream 100 bases:

>100_bases
ACTGAAGGAGGGCAATGGTTTGAAGCAAAGGAATGTCAGCGACGTAAGGGGCAGGTCAAACATCCTGCTCCGGCCAGTAG
AAACAGCTTTTTTACTATTA

Downstream 100 bases:

>100_bases
ACGGACAACATAGCCCCGTCGCGGCCCGGTAATCGAACAGCGGCGGGGCTTGTTTATCTCTTCTAGGGTTAGTCCCGTGG
TTTTATAAGGGTATCTGTAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MFLAAGTVLARPALAAAGDAVDTGDTAFILVASALVMLMTPGLAFFYGGMVRQKNVLGIIMQCLATLALISVQWVLWGYS
LAFGPDHLHLLGGLDWAGFRGVGLEPNPLYAATIPHQVFAMFQLMFAIITPALITGAFAERMRFPAFLLFTLMWATLVYD
PLAHWVWGAGGWLRELGALDFAGGTVVHISAGVAGLVCALVLGRRRDYGRIPLPPHNLPMTVLGAALLWFGWFGFNAGSA
LGANGLAGNAFVTTNTAAGAAILGWLAVEWLLHGKPTALGAASGAVAGLVAITPAAGFVTPPAALVIGGIGGALCCLSVA
VAKVKLGYDDSLDAFGCHGVGGTWGAIATGIFATKAVNPAGADGLLYGNAHLLGVQVVGVAAAWIFTAAVTYGILKVVSL
LTPLRVTAEEEEAGLDLSQHGESAYPDTGLAALEVAEGKRVTAPGELAVNWMVSQGNPAGTGLFRQE

Sequences:

>Translated_467_residues
MFLAAGTVLARPALAAAGDAVDTGDTAFILVASALVMLMTPGLAFFYGGMVRQKNVLGIIMQCLATLALISVQWVLWGYS
LAFGPDHLHLLGGLDWAGFRGVGLEPNPLYAATIPHQVFAMFQLMFAIITPALITGAFAERMRFPAFLLFTLMWATLVYD
PLAHWVWGAGGWLRELGALDFAGGTVVHISAGVAGLVCALVLGRRRDYGRIPLPPHNLPMTVLGAALLWFGWFGFNAGSA
LGANGLAGNAFVTTNTAAGAAILGWLAVEWLLHGKPTALGAASGAVAGLVAITPAAGFVTPPAALVIGGIGGALCCLSVA
VAKVKLGYDDSLDAFGCHGVGGTWGAIATGIFATKAVNPAGADGLLYGNAHLLGVQVVGVAAAWIFTAAVTYGILKVVSL
LTPLRVTAEEEEAGLDLSQHGESAYPDTGLAALEVAEGKRVTAPGELAVNWMVSQGNPAGTGLFRQE
>Mature_467_residues
MFLAAGTVLARPALAAAGDAVDTGDTAFILVASALVMLMTPGLAFFYGGMVRQKNVLGIIMQCLATLALISVQWVLWGYS
LAFGPDHLHLLGGLDWAGFRGVGLEPNPLYAATIPHQVFAMFQLMFAIITPALITGAFAERMRFPAFLLFTLMWATLVYD
PLAHWVWGAGGWLRELGALDFAGGTVVHISAGVAGLVCALVLGRRRDYGRIPLPPHNLPMTVLGAALLWFGWFGFNAGSA
LGANGLAGNAFVTTNTAAGAAILGWLAVEWLLHGKPTALGAASGAVAGLVAITPAAGFVTPPAALVIGGIGGALCCLSVA
VAKVKLGYDDSLDAFGCHGVGGTWGAIATGIFATKAVNPAGADGLLYGNAHLLGVQVVGVAAAWIFTAAVTYGILKVVSL
LTPLRVTAEEEEAGLDLSQHGESAYPDTGLAALEVAEGKRVTAPGELAVNWMVSQGNPAGTGLFRQE

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=422, Percent_Identity=47.1563981042654, Blast_Score=338, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17550182, Length=475, Percent_Identity=30.5263157894737, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17550180, Length=436, Percent_Identity=28.8990825688073, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=460, Percent_Identity=26.7391304347826, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=28.7383177570093, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=40.1891252955083, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=37.3205741626794, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=34.9282296650718, Blast_Score=232, Evalue=7e-62,
Organism=Drosophila melanogaster, GI161078314, Length=411, Percent_Identity=32.1167883211679, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 48058; Mature: 48058

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLAAGTVLARPALAAAGDAVDTGDTAFILVASALVMLMTPGLAFFYGGMVRQKNVLGII
CEEECCHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHH
MQCLATLALISVQWVLWGYSLAFGPDHLHLLGGLDWAGFRGVGLEPNPLYAATIPHQVFA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHH
MFQLMFAIITPALITGAFAERMRFPAFLLFTLMWATLVYDPLAHWVWGAGGWLRELGALD
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
FAGGTVVHISAGVAGLVCALVLGRRRDYGRIPLPPHNLPMTVLGAALLWFGWFGFNAGSA
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
LGANGLAGNAFVTTNTAAGAAILGWLAVEWLLHGKPTALGAASGAVAGLVAITPAAGFVT
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEECCCCCCCC
PPAALVIGGIGGALCCLSVAVAKVKLGYDDSLDAFGCHGVGGTWGAIATGIFATKAVNPA
CCHHHEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GADGLLYGNAHLLGVQVVGVAAAWIFTAAVTYGILKVVSLLTPLRVTAEEEEAGLDLSQH
CCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCHHHC
GESAYPDTGLAALEVAEGKRVTAPGELAVNWMVSQGNPAGTGLFRQE
CCCCCCCCCCEEEEECCCCEECCCCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MFLAAGTVLARPALAAAGDAVDTGDTAFILVASALVMLMTPGLAFFYGGMVRQKNVLGII
CEEECCHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHH
MQCLATLALISVQWVLWGYSLAFGPDHLHLLGGLDWAGFRGVGLEPNPLYAATIPHQVFA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHH
MFQLMFAIITPALITGAFAERMRFPAFLLFTLMWATLVYDPLAHWVWGAGGWLRELGALD
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
FAGGTVVHISAGVAGLVCALVLGRRRDYGRIPLPPHNLPMTVLGAALLWFGWFGFNAGSA
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
LGANGLAGNAFVTTNTAAGAAILGWLAVEWLLHGKPTALGAASGAVAGLVAITPAAGFVT
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEECCCCCCCC
PPAALVIGGIGGALCCLSVAVAKVKLGYDDSLDAFGCHGVGGTWGAIATGIFATKAVNPA
CCHHHEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GADGLLYGNAHLLGVQVVGVAAAWIFTAAVTYGILKVVSLLTPLRVTAEEEEAGLDLSQH
CCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCHHHC
GESAYPDTGLAALEVAEGKRVTAPGELAVNWMVSQGNPAGTGLFRQE
CCCCCCCCCCEEEEECCCCEECCCCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]