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The map label for this gene is pnp

Identifier: 83595114

GI number: 83595114

Start: 4334832

End: 4336958

Strand: Direct

Name: pnp

Synonym: Rru_A3785

Alternate gene names: 83595114

Gene position: 4334832-4336958 (Clockwise)

Preceding gene: 83595113

Following gene: 83595115

Centisome position: 99.59

GC content: 64.55

Gene sequence:

>2127_bases
ATGTCTCTGTTCAACGTGTTCCGCAAGGAAATCGAATGGGGCGGGCGCAAGCTGGTCCTCGAGACCGGGCGCATCGCCCG
TCAGGCCGATGGCGCGGTGATGGCCAGCCTGGGCGACACCACGGTGCTGTGCACCGCCGTCGGCGCGAAGACGAAGTCGA
AGTTCGATTTCTTTCCGCTGACCGTGAACTACCAGGAAAAGACGTTCGCCGCCGGTAAGATCCCGGGCGGTTTCTTCAAG
CGCGAGGGTCGTCCGAGCGAGAAGGAAACCCTGGTTTCCCGCCTGATCGACCGGCCGATCCGGCCGCTGTTCGTTCATGG
CTACAAGAACGAAACCCAGCTGGTTTGCACCGTTCTGTGCCACGACCTTGAAAACAACCCCGATATCGTCGCCATCGTCG
GCGCCTCGGCGGCTTTGACCCTGTCGGGCCTGCCCTTCCTCGGCCCCATCGGCGCCGCCCGCGTCGGTCTGATCGACGGC
GCCTTCGTGCTGAACCCGACCCGTGATCAGATGGCCGACTCGGTCCTCGATCTGGTGGTCGCGGGCACCCGCGAAGGCGT
GCTGATGGTCGAATCCGAGGCCCACGAGCTGTCCGAGCAGCAGATGCTCGACGCGGTGATGTTCGGCCACGAGGGCTATC
AGCCCGTCATCGACGCCATCATCGCCCTGGCCGAACAGTGCGCCCGCGAGCCCCGCGCCATCGAGCCGCCGGCCCCCGAG
GCCGAGGCCGTGGCCGCCAAGGTTCGTGAAGTCGGCGAAGCCGGTCTGCGCGACGCCTATAAAGAAGCCGACAAGATGGT
CCGCCACGACAAGGTGGATGCCGTCCGCGACGCCGTGACCGCCGCCTTCGCCGGCGACGACGCCGCCCTCGCCCTGGCCG
GCGCCGCCTTCAAGGATCTGGAGAAGGACATCGTCCGCGGCTCCATCCTTGATACCGGCCTGCGCATCGACGGTCGCGAC
ACCAAGACCGTGCGCCCGATCGAAATCTATCCGGGCATCCTGCCGCGCGCCCATGGTTCGGCGCTGTTCACCCGGGGCGA
GACCCAGGCGCTGGTCACCACCACGCTCGGCACCGGCCAGGATGAGCAGATCATCGACGCCCTGGAAGGCGAATACCGCG
AAAACTTCATGCTGCACTACAACTTCCCCCCCTATTCGGTGGGCGAGGCCGGGCGCATGGGGTCTCCGGGCCGTCGTGAA
ATCGGTCATGGCAAGCTGGCTTGGCGCGCCATCCATCCGATGATGCCGGCCAAGGACGCCTTCCCCTACACCGTCCGCGT
CGTCTCCGAGATCACCGAGTCGAACGGTTCGTCGTCGATGGCCACCGTCTGCGGCACCTCGCTCGCCCTGATGGATGCCG
GCGTGCCGCTCAAGAACGCCGTGGCCGGTATCGCCATGGGCCTGATCAAGGAAGACGAGCGCTTCGCCGTGCTCTCCGAC
ATCCTGGGCGACGAGGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGTACCGCCAATGGTATCACCAGCTTGCAGAT
GGACATCAAGATCACCTCGATCACCAAGGAGATCATGAATGTCGCGCTCAACCAGGCGAAGGACGGGCGCATCCATATCC
TGGGCGAGATGACCAAGGCCCTGGGCAACGCCCGGTCGGACGTGTCCGATTTCGCGCCGCGCATCACCACCATCAAGGTG
CCGCCGCAGAAGGTTCGCGAGGTCATCGGTTCGGGCGGCAAGGTCATCCGCGAGATCACCGAAGTCACCGGCACCAAGAT
CGATATCGAAGACGACGGCACGATCAAGATCGCCTCGGCCGATGCCGAAGCGACCCAGCGCGCCGTCGATTGGATCAAGG
GCATCGTCGCCGAGCCCGAGATTGGCGTGGTCTACACCGGCAAGGTGGTCAAGATCATGGACTTCGGCGCCTTCGTGAAC
TTCCTCGGCACCCGCGACGGTCTCGTCCATATTTCCGAGCTGTCCCAGGACCGGGTCAAGAAGGTCGGCGATGTGGTCAA
TGTCGGCGATCAGGTGAAGGTGAAGTGCGTCGGCTTTGACGATCGCGGCAAGATCAAGCTGTCGATGAAGCAGGTCGATC
AGGTCACCGGCGCCGATTTGTCGACGCAGGCCCCCGCGGAAGAGTAA

Upstream 100 bases:

>100_bases
GGTCTCCGGCCGCCGTGGCATACGGAAGGCTTTCCTTCCGAATCCCCCGGCGGCTGGCGCAATCCGGCGGCGATGGATAC
GAAAAAGGATGAAGAAACGC

Downstream 100 bases:

>100_bases
CAACCCGACCGACGCGCGGAGAAGGCGTGTCCGGCCTTAAGGGAAAGACCATGTCCGACCGCTCTCAGACCGATCGTCCC
GGTCTTTCTGGGACTCCTTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MSLFNVFRKEIEWGGRKLVLETGRIARQADGAVMASLGDTTVLCTAVGAKTKSKFDFFPLTVNYQEKTFAAGKIPGGFFK
REGRPSEKETLVSRLIDRPIRPLFVHGYKNETQLVCTVLCHDLENNPDIVAIVGASAALTLSGLPFLGPIGAARVGLIDG
AFVLNPTRDQMADSVLDLVVAGTREGVLMVESEAHELSEQQMLDAVMFGHEGYQPVIDAIIALAEQCAREPRAIEPPAPE
AEAVAAKVREVGEAGLRDAYKEADKMVRHDKVDAVRDAVTAAFAGDDAALALAGAAFKDLEKDIVRGSILDTGLRIDGRD
TKTVRPIEIYPGILPRAHGSALFTRGETQALVTTTLGTGQDEQIIDALEGEYRENFMLHYNFPPYSVGEAGRMGSPGRRE
IGHGKLAWRAIHPMMPAKDAFPYTVRVVSEITESNGSSSMATVCGTSLALMDAGVPLKNAVAGIAMGLIKEDERFAVLSD
ILGDEDHLGDMDFKVAGTANGITSLQMDIKITSITKEIMNVALNQAKDGRIHILGEMTKALGNARSDVSDFAPRITTIKV
PPQKVREVIGSGGKVIREITEVTGTKIDIEDDGTIKIASADAEATQRAVDWIKGIVAEPEIGVVYTGKVVKIMDFGAFVN
FLGTRDGLVHISELSQDRVKKVGDVVNVGDQVKVKCVGFDDRGKIKLSMKQVDQVTGADLSTQAPAEE

Sequences:

>Translated_708_residues
MSLFNVFRKEIEWGGRKLVLETGRIARQADGAVMASLGDTTVLCTAVGAKTKSKFDFFPLTVNYQEKTFAAGKIPGGFFK
REGRPSEKETLVSRLIDRPIRPLFVHGYKNETQLVCTVLCHDLENNPDIVAIVGASAALTLSGLPFLGPIGAARVGLIDG
AFVLNPTRDQMADSVLDLVVAGTREGVLMVESEAHELSEQQMLDAVMFGHEGYQPVIDAIIALAEQCAREPRAIEPPAPE
AEAVAAKVREVGEAGLRDAYKEADKMVRHDKVDAVRDAVTAAFAGDDAALALAGAAFKDLEKDIVRGSILDTGLRIDGRD
TKTVRPIEIYPGILPRAHGSALFTRGETQALVTTTLGTGQDEQIIDALEGEYRENFMLHYNFPPYSVGEAGRMGSPGRRE
IGHGKLAWRAIHPMMPAKDAFPYTVRVVSEITESNGSSSMATVCGTSLALMDAGVPLKNAVAGIAMGLIKEDERFAVLSD
ILGDEDHLGDMDFKVAGTANGITSLQMDIKITSITKEIMNVALNQAKDGRIHILGEMTKALGNARSDVSDFAPRITTIKV
PPQKVREVIGSGGKVIREITEVTGTKIDIEDDGTIKIASADAEATQRAVDWIKGIVAEPEIGVVYTGKVVKIMDFGAFVN
FLGTRDGLVHISELSQDRVKKVGDVVNVGDQVKVKCVGFDDRGKIKLSMKQVDQVTGADLSTQAPAEE
>Mature_707_residues
SLFNVFRKEIEWGGRKLVLETGRIARQADGAVMASLGDTTVLCTAVGAKTKSKFDFFPLTVNYQEKTFAAGKIPGGFFKR
EGRPSEKETLVSRLIDRPIRPLFVHGYKNETQLVCTVLCHDLENNPDIVAIVGASAALTLSGLPFLGPIGAARVGLIDGA
FVLNPTRDQMADSVLDLVVAGTREGVLMVESEAHELSEQQMLDAVMFGHEGYQPVIDAIIALAEQCAREPRAIEPPAPEA
EAVAAKVREVGEAGLRDAYKEADKMVRHDKVDAVRDAVTAAFAGDDAALALAGAAFKDLEKDIVRGSILDTGLRIDGRDT
KTVRPIEIYPGILPRAHGSALFTRGETQALVTTTLGTGQDEQIIDALEGEYRENFMLHYNFPPYSVGEAGRMGSPGRREI
GHGKLAWRAIHPMMPAKDAFPYTVRVVSEITESNGSSSMATVCGTSLALMDAGVPLKNAVAGIAMGLIKEDERFAVLSDI
LGDEDHLGDMDFKVAGTANGITSLQMDIKITSITKEIMNVALNQAKDGRIHILGEMTKALGNARSDVSDFAPRITTIKVP
PQKVREVIGSGGKVIREITEVTGTKIDIEDDGTIKIASADAEATQRAVDWIKGIVAEPEIGVVYTGKVVKIMDFGAFVNF
LGTRDGLVHISELSQDRVKKVGDVVNVGDQVKVKCVGFDDRGKIKLSMKQVDQVTGADLSTQAPAEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=707, Percent_Identity=39.1796322489392, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI4826690, Length=88, Percent_Identity=54.5454545454545, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI21361576, Length=86, Percent_Identity=44.1860465116279, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=58.5260115606936, Blast_Score=802, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=697, Percent_Identity=33.8593974175036, Blast_Score=359, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17535281, Length=85, Percent_Identity=49.4117647058824, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320850, Length=108, Percent_Identity=39.8148148148148, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=39.6600566572238, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=39.6600566572238, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=39.6600566572238, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=39.4817073170732, Blast_Score=434, Evalue=1e-121,
Organism=Drosophila melanogaster, GI20129977, Length=99, Percent_Identity=42.4242424242424, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_RHORT (Q2RMR6)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428866.1
- ProteinModelPortal:   Q2RMR6
- SMR:   Q2RMR6
- STRING:   Q2RMR6
- GeneID:   3837242
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3785
- NMPDR:   fig|1085.1.peg.1130
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q2RMR6
- ProtClustDB:   PRK11824
- BioCyc:   RRUB269796:RRU_A3785-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76110; Mature: 75979

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFNVFRKEIEWGGRKLVLETGRIARQADGAVMASLGDTTVLCTAVGAKTKSKFDFFPL
CCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCEEEECCCCEEEEEECCCCCCCCCEEEEE
TVNYQEKTFAAGKIPGGFFKREGRPSEKETLVSRLIDRPIRPLFVHGYKNETQLVCTVLC
EEECCHHEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
HDLENNPDIVAIVGASAALTLSGLPFLGPIGAARVGLIDGAFVLNPTRDQMADSVLDLVV
HHCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHEEEECCEEEECCCHHHHHHHHHHHHH
AGTREGVLMVESEAHELSEQQMLDAVMFGHEGYQPVIDAIIALAEQCAREPRAIEPPAPE
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AEAVAAKVREVGEAGLRDAYKEADKMVRHDKVDAVRDAVTAAFAGDDAALALAGAAFKDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EKDIVRGSILDTGLRIDGRDTKTVRPIEIYPGILPRAHGSALFTRGETQALVTTTLGTGQ
HHHHHHCHHHHCCCEECCCCCCEECEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCC
DEQIIDALEGEYRENFMLHYNFPPYSVGEAGRMGSPGRREIGHGKLAWRAIHPMMPAKDA
CHHHHHHHCCHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
FPYTVRVVSEITESNGSSSMATVCGTSLALMDAGVPLKNAVAGIAMGLIKEDERFAVLSD
CCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
ILGDEDHLGDMDFKVAGTANGITSLQMDIKITSITKEIMNVALNQAKDGRIHILGEMTKA
HCCCCCCCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
LGNARSDVSDFAPRITTIKVPPQKVREVIGSGGKVIREITEVTGTKIDIEDDGTIKIASA
HCCCCCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEEC
DAEATQRAVDWIKGIVAEPEIGVVYTGKVVKIMDFGAFVNFLGTRDGLVHISELSQDRVK
CHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHHHHH
KVGDVVNVGDQVKVKCVGFDDRGKIKLSMKQVDQVTGADLSTQAPAEE
HHHHHHCCCCCEEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SLFNVFRKEIEWGGRKLVLETGRIARQADGAVMASLGDTTVLCTAVGAKTKSKFDFFPL
CHHHHHHHHHHCCCCEEEEECCCHHHHCCCCEEEECCCCEEEEEECCCCCCCCCEEEEE
TVNYQEKTFAAGKIPGGFFKREGRPSEKETLVSRLIDRPIRPLFVHGYKNETQLVCTVLC
EEECCHHEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
HDLENNPDIVAIVGASAALTLSGLPFLGPIGAARVGLIDGAFVLNPTRDQMADSVLDLVV
HHCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHEEEECCEEEECCCHHHHHHHHHHHHH
AGTREGVLMVESEAHELSEQQMLDAVMFGHEGYQPVIDAIIALAEQCAREPRAIEPPAPE
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AEAVAAKVREVGEAGLRDAYKEADKMVRHDKVDAVRDAVTAAFAGDDAALALAGAAFKDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EKDIVRGSILDTGLRIDGRDTKTVRPIEIYPGILPRAHGSALFTRGETQALVTTTLGTGQ
HHHHHHCHHHHCCCEECCCCCCEECEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCC
DEQIIDALEGEYRENFMLHYNFPPYSVGEAGRMGSPGRREIGHGKLAWRAIHPMMPAKDA
CHHHHHHHCCHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
FPYTVRVVSEITESNGSSSMATVCGTSLALMDAGVPLKNAVAGIAMGLIKEDERFAVLSD
CCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
ILGDEDHLGDMDFKVAGTANGITSLQMDIKITSITKEIMNVALNQAKDGRIHILGEMTKA
HCCCCCCCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
LGNARSDVSDFAPRITTIKVPPQKVREVIGSGGKVIREITEVTGTKIDIEDDGTIKIASA
HCCCCCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEEC
DAEATQRAVDWIKGIVAEPEIGVVYTGKVVKIMDFGAFVNFLGTRDGLVHISELSQDRVK
CHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHHHHH
KVGDVVNVGDQVKVKCVGFDDRGKIKLSMKQVDQVTGADLSTQAPAEE
HHHHHHCCCCCEEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA