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The map label for this gene is truB

Identifier: 83595112

GI number: 83595112

Start: 4333312

End: 4334238

Strand: Direct

Name: truB

Synonym: Rru_A3783

Alternate gene names: 83595112

Gene position: 4333312-4334238 (Clockwise)

Preceding gene: 83595111

Following gene: 83595113

Centisome position: 99.55

GC content: 64.4

Gene sequence:

>927_bases
ATGAGCCGGCGCCGGCGCGGTCAGGCGATCCACGGCTGGCTTGCCATCGACAAGCCCGTCGGCATCACCAGCACCGATGT
GGTCAACCGGCTGCGGCGGACGATGGACGCGGCCAAGGTCGGCCATGGCGGCACCCTTGATCCGTTGGCCAGCGGCGTTC
TGCCCATCGCCTTTGGCGAGGCGACCAAGACCGTGTCCTATGTGATGGACGGCACCAAGGTCTATCACATGGTCGTCGGC
TGGGGTGAAGCCCGCGATACCGACGATCGCGAAGGCAAGGTCATCGACACCAGCGATGTCCGTCCCGATGCCCAGGCGAT
CACCGCCATGCTGGCGCGCTTCACCGGCGAGATCGAACAGGTGCCGCCGATCTATTCGGCCGTCAAGGTCGAGGGGCGGC
GCTCCTATGATCTGGCGCGCGCCGATCAGGCCGTGGAATTGAAGTCGCGCAAGATCCTGATCAAGGATCTGACGCTTCTC
GAGACCTCGACCCCGGATTTTGGCCATTTCCGCATCGTTTCGGGCAAGGGCGCCTATATGCGCTCGCTGGCCCGCGACCT
GGCCTTGGCCCTGGGAACGGTCGGCCATGTCGTCGCCCTGCGTCGTCTCGCATGCGGTCCGTTCACCGCCGAAACGGCGA
TTTCTCTGGATATGGTAGACGCCCTCGGGCATGATGCGCCCCATTCTGAAAGCCTGCTTCCCATCGAGGCCGCGCTGGCC
GACATCCCGGCCGTGCCTTTGACGGAAGCGGAGGCCCGTCGCCTGTCCCAAGGACAGCCCGTGTCCCTCTTGCATCTGGC
AAGCCGGACCCCCCTGAACGGACTCGCCCAAGGCGAAACCGTTCAGGCGCTGCTCAATGGAAAACTGGTGGCTTTGGCAA
AAATCGTAGAGGGCGAAATCCGCCCCTTGCGCGTCGTCAACCTCTAG

Upstream 100 bases:

>100_bases
CGGGCGAGGATGACGGGGATGACGAGGCGGACGATGAAGACGACGACGGCGGCGAAGAGGGCCCCGGCGCGGCCGCCCCG
CCGCCGGCGGACGAGGGACG

Downstream 100 bases:

>100_bases
GCCGATCCCCACGCGGGACGGCGCTGGAAACAAGGAGATCATACCGATGTCGATCACGCCCGAGCGCAAGCAGGAAATGA
TCAAGGATTACGCCACGAAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSRRRRGQAIHGWLAIDKPVGITSTDVVNRLRRTMDAAKVGHGGTLDPLASGVLPIAFGEATKTVSYVMDGTKVYHMVVG
WGEARDTDDREGKVIDTSDVRPDAQAITAMLARFTGEIEQVPPIYSAVKVEGRRSYDLARADQAVELKSRKILIKDLTLL
ETSTPDFGHFRIVSGKGAYMRSLARDLALALGTVGHVVALRRLACGPFTAETAISLDMVDALGHDAPHSESLLPIEAALA
DIPAVPLTEAEARRLSQGQPVSLLHLASRTPLNGLAQGETVQALLNGKLVALAKIVEGEIRPLRVVNL

Sequences:

>Translated_308_residues
MSRRRRGQAIHGWLAIDKPVGITSTDVVNRLRRTMDAAKVGHGGTLDPLASGVLPIAFGEATKTVSYVMDGTKVYHMVVG
WGEARDTDDREGKVIDTSDVRPDAQAITAMLARFTGEIEQVPPIYSAVKVEGRRSYDLARADQAVELKSRKILIKDLTLL
ETSTPDFGHFRIVSGKGAYMRSLARDLALALGTVGHVVALRRLACGPFTAETAISLDMVDALGHDAPHSESLLPIEAALA
DIPAVPLTEAEARRLSQGQPVSLLHLASRTPLNGLAQGETVQALLNGKLVALAKIVEGEIRPLRVVNL
>Mature_307_residues
SRRRRGQAIHGWLAIDKPVGITSTDVVNRLRRTMDAAKVGHGGTLDPLASGVLPIAFGEATKTVSYVMDGTKVYHMVVGW
GEARDTDDREGKVIDTSDVRPDAQAITAMLARFTGEIEQVPPIYSAVKVEGRRSYDLARADQAVELKSRKILIKDLTLLE
TSTPDFGHFRIVSGKGAYMRSLARDLALALGTVGHVVALRRLACGPFTAETAISLDMVDALGHDAPHSESLLPIEAALAD
IPAVPLTEAEARRLSQGQPVSLLHLASRTPLNGLAQGETVQALLNGKLVALAKIVEGEIRPLRVVNL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=252, Percent_Identity=34.5238095238095, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI2367200, Length=320, Percent_Identity=35.3125, Blast_Score=185, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=29.2307692307692, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=236, Percent_Identity=28.3898305084746, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364189, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364187, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364185, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364183, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62471759, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17975520, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_RHORT (Q2RMR8)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428864.1
- ProteinModelPortal:   Q2RMR8
- SMR:   Q2RMR8
- STRING:   Q2RMR8
- GeneID:   3837240
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3783
- NMPDR:   fig|1085.1.peg.1132
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q2RMR8
- ProtClustDB:   PRK05389
- BioCyc:   RRUB269796:RRU_A3783-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33086; Mature: 32954

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRRGQAIHGWLAIDKPVGITSTDVVNRLRRTMDAAKVGHGGTLDPLASGVLPIAFGE
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECC
ATKTVSYVMDGTKVYHMVVGWGEARDTDDREGKVIDTSDVRPDAQAITAMLARFTGEIEQ
HHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCHHH
VPPIYSAVKVEGRRSYDLARADQAVELKSRKILIKDLTLLETSTPDFGHFRIVSGKGAYM
CCCHHHHEEECCCCCCCHHHHCHHHHHHCCEEEEEEEEEEECCCCCCCCEEEECCCCHHH
RSLARDLALALGTVGHVVALRRLACGPFTAETAISLDMVDALGHDAPHSESLLPIEAALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DIPAVPLTEAEARRLSQGQPVSLLHLASRTPLNGLAQGETVQALLNGKLVALAKIVEGEI
HCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCC
RPLRVVNL
CCEEEECC
>Mature Secondary Structure 
SRRRRGQAIHGWLAIDKPVGITSTDVVNRLRRTMDAAKVGHGGTLDPLASGVLPIAFGE
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECC
ATKTVSYVMDGTKVYHMVVGWGEARDTDDREGKVIDTSDVRPDAQAITAMLARFTGEIEQ
HHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCHHH
VPPIYSAVKVEGRRSYDLARADQAVELKSRKILIKDLTLLETSTPDFGHFRIVSGKGAYM
CCCHHHHEEECCCCCCCHHHHCHHHHHHCCEEEEEEEEEEECCCCCCCCEEEECCCCHHH
RSLARDLALALGTVGHVVALRRLACGPFTAETAISLDMVDALGHDAPHSESLLPIEAALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DIPAVPLTEAEARRLSQGQPVSLLHLASRTPLNGLAQGETVQALLNGKLVALAKIVEGEI
HCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCC
RPLRVVNL
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA