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The map label for this gene is trmB

Identifier: 83595106

GI number: 83595106

Start: 4325901

End: 4326668

Strand: Direct

Name: trmB

Synonym: Rru_A3777

Alternate gene names: 83595106

Gene position: 4325901-4326668 (Clockwise)

Preceding gene: 83595105

Following gene: 83595107

Centisome position: 99.38

GC content: 69.27

Gene sequence:

>768_bases
GTGCCTGAAAAACCGACAGAGGACGCGCGCTTTTACGGCCGACGCAAGGGCAAGCCGCTTCGCGCCACTCGTCAGCGTCT
TCTCGACACCATGCTGCCCGCCCTCACCTTGCCCCTCGCCGGGCTGGGCGAGGGCGAGCGACTCGACCCGCGTGGACTGT
TTCCGCGACCGGTCGATCAGCTGTGGCTTGAAATCGGCTTTGGCGGGGGCGAGCATCTGGTCGCCCAGGCCGCCGCCCAT
CCCGAGGTCGGCCTGATCGGCTCGGAGGTCTTCGCCTATGGAGTCGGCAAGGCGCTCAGCCAGATCGACGAAACGGGGGT
CGATAATATCCGGCTGTGGCCCGAGGACGTGCGTCAGGTCTTGCCCGCCCTGCCCGACGGCTGCCTTCAGCGGCTGTTCG
TGCTGTTTCCCGATCCCTGGCCCAAGCGCCGCCATGCCCGGCGGCGGATGATCCAGCCCGCCCGCCTTGACGATTTCGCC
CGGCTGCTGGCCGATGGCGGCGAGTTGCGGGTGGCCAGCGATGACATGGGCTATGTCCGCTGGACCCTGATGCATGTCAC
CGCCCATCCGGCCTTCCGCTGGACCGCCCAGGGGCCGACCGACTGGCGCGAGCGGCCGGCCGATTGGGTGGAGACCCGCT
ATGAGGCCAAGGCGCTGCAGGCCGGCCGCAAACCGGCCTATCTGATCTTTCGCCGCCGTCCCCGCGCGGCGGACCCCGGG
ACCCTCGCCGAAGCCCCGGCCGGGGAAGGCGCCGATAACAATCCTTGA

Upstream 100 bases:

>100_bases
CGCACCGCCGCCTATGGCCATTTCGGGCGCAAGCCTGAAAAGGATGGCGGCTTCTCGTGGGAGCGCACCGATCTGGTCGC
CGGTTTGAAGACCGCCTTCG

Downstream 100 bases:

>100_bases
GATTGGTCGAAACTGGCCTTATAAAGACCGTGACATCTTCTTGACATGGCCTTGTGGGGCTGGCGGGGGGTGGGCCGTCG
GCCCACCTTTTTTGTTTTCG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPEKPTEDARFYGRRKGKPLRATRQRLLDTMLPALTLPLAGLGEGERLDPRGLFPRPVDQLWLEIGFGGGEHLVAQAAAH
PEVGLIGSEVFAYGVGKALSQIDETGVDNIRLWPEDVRQVLPALPDGCLQRLFVLFPDPWPKRRHARRRMIQPARLDDFA
RLLADGGELRVASDDMGYVRWTLMHVTAHPAFRWTAQGPTDWRERPADWVETRYEAKALQAGRKPAYLIFRRRPRAADPG
TLAEAPAGEGADNNP

Sequences:

>Translated_255_residues
MPEKPTEDARFYGRRKGKPLRATRQRLLDTMLPALTLPLAGLGEGERLDPRGLFPRPVDQLWLEIGFGGGEHLVAQAAAH
PEVGLIGSEVFAYGVGKALSQIDETGVDNIRLWPEDVRQVLPALPDGCLQRLFVLFPDPWPKRRHARRRMIQPARLDDFA
RLLADGGELRVASDDMGYVRWTLMHVTAHPAFRWTAQGPTDWRERPADWVETRYEAKALQAGRKPAYLIFRRRPRAADPG
TLAEAPAGEGADNNP
>Mature_254_residues
PEKPTEDARFYGRRKGKPLRATRQRLLDTMLPALTLPLAGLGEGERLDPRGLFPRPVDQLWLEIGFGGGEHLVAQAAAHP
EVGLIGSEVFAYGVGKALSQIDETGVDNIRLWPEDVRQVLPALPDGCLQRLFVLFPDPWPKRRHARRRMIQPARLDDFAR
LLADGGELRVASDDMGYVRWTLMHVTAHPAFRWTAQGPTDWRERPADWVETRYEAKALQAGRKPAYLIFRRRPRAADPGT
LAEAPAGEGADNNP

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=230, Percent_Identity=30.8695652173913, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_RHORT (Q2RMS4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428858.1
- ProteinModelPortal:   Q2RMS4
- SMR:   Q2RMS4
- STRING:   Q2RMS4
- GeneID:   3837234
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3777
- NMPDR:   fig|1085.1.peg.1138
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   YEAKALR
- PhylomeDB:   Q2RMS4
- ProtClustDB:   PRK00121
- BioCyc:   RRUB269796:RRU_A3777-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28578; Mature: 28447

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: BINDING 64-64 BINDING 89-89 BINDING 116-116 BINDING 138-138 BINDING 142-142 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKPTEDARFYGRRKGKPLRATRQRLLDTMLPALTLPLAGLGEGERLDPRGLFPRPVDQ
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
LWLEIGFGGGEHLVAQAAAHPEVGLIGSEVFAYGVGKALSQIDETGVDNIRLWPEDVRQV
HHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHH
LPALPDGCLQRLFVLFPDPWPKRRHARRRMIQPARLDDFARLLADGGELRVASDDMGYVR
HHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCHHE
WTLMHVTAHPAFRWTAQGPTDWRERPADWVETRYEAKALQAGRKPAYLIFRRRPRAADPG
EEEEHEECCCCEEEECCCCCCHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
TLAEAPAGEGADNNP
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
PEKPTEDARFYGRRKGKPLRATRQRLLDTMLPALTLPLAGLGEGERLDPRGLFPRPVDQ
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
LWLEIGFGGGEHLVAQAAAHPEVGLIGSEVFAYGVGKALSQIDETGVDNIRLWPEDVRQV
HHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHH
LPALPDGCLQRLFVLFPDPWPKRRHARRRMIQPARLDDFARLLADGGELRVASDDMGYVR
HHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCHHE
WTLMHVTAHPAFRWTAQGPTDWRERPADWVETRYEAKALQAGRKPAYLIFRRRPRAADPG
EEEEHEECCCCEEEECCCCCCHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
TLAEAPAGEGADNNP
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA