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The map label for this gene is metK2

Identifier: 83595105

GI number: 83595105

Start: 4324739

End: 4325908

Strand: Direct

Name: metK2

Synonym: Rru_A3776

Alternate gene names: 83595105

Gene position: 4324739-4325908 (Clockwise)

Preceding gene: 83595104

Following gene: 83595106

Centisome position: 99.35

GC content: 64.87

Gene sequence:

>1170_bases
GTGGCCCAGTCCGAATACCTTTTCACCAGCGAGTCCGTGTCCGAAGGGCATCCCGACAAGGTTTGCGACCGGATTTCGGA
CGCGATCGTCGATGCTTTTCTTGCCGAGGATCCCCATTCCCGGGTCGCCCTGGAAACCATGGCGACGACCAATTTCGTGG
TGCTGGCCGGCGAGGTCCGCGGGCCCGACAGCCTGACTCACGACCGGCTGAAGGAGATCGCCCGCGAGGCGATCAAGGAT
ATCGGCTATGAGCAGCGCGGCTTCCATTGGAAGGACGCCGAGATCGTCTCCCACGTCCATTCCCAGTCCGCCGATATCGC
CGTCGGCGTTGACGCCGCCGGCAATAAGGACGAGGGCGCGGGCGATCAGGGCATCATGTTCGGCTATGCCTGCACCGAAA
CCGAAGAGCTGATGCCGGCGCCGATCGCCCTCTCCCACGCGATCCTCAAGTCGCTGGCCGAGTTCCGCCACGGCGGCGAC
ACCAGCTTCGGCCCGGATTCCAAAAGCCAGGTCACCCTGCGCTATGTCGATGGCAAGCCGGTGGGCGCCGCCAGCGTCGT
CGTCTCGACCCAGCACGCCGGTAATCTTAGCCAGGACGAGGTCCGCGAACTGGTGCGGCCGCATGTGCTGAAGGTTCTGC
CCGAAGGCTGGATGTGTCCCGAAGAGGAATTCTACGTCAATCCGACCGGCCGCTTCGTCATCGGCGGACCGGACGGCGAT
TGCGGACTGACCGGGCGCAAGATCATCGTCGACACCTATGGCGGCGCGGCTCCCCATGGCGGCGGCGCCTTCTCGGGCAA
GGACCCGACCAAAGTCGACCGTTCGGCCGCCTATGCCGCGCGCTATCTGGCCAAGAACGTCGTCGCCGCCGGGCTGGCCG
AGAAATGCGTGATCCAGGTGTCCTATGCCATCGGCGTGTCCAAGCCGCTGTCGGTCTATGTCAACACCCAGGGCACCGGT
CAGGTCGACGAGCAGCGTCTGGCCGTCGTTCTTCAGCAGTTGATGGATCTGAGCCCGCGCGGCATCCGCCAGCATCTGCA
GCTGTCGCGTCCGATCTACGCCCGCACCGCCGCCTATGGCCATTTCGGGCGCAAGCCTGAAAAGGATGGCGGCTTCTCGT
GGGAGCGCACCGATCTGGTCGCCGGTTTGAAGACCGCCTTCGGTGCCTGA

Upstream 100 bases:

>100_bases
GCCTTCCCAGGGCCGACATGCCAAAAAACCCCCTTCAATCGTTGGGAGAAGGACGCCGTCCGCGCCCTTCTCCCAATCCG
ATCCGGGGTTGGAGGAAACA

Downstream 100 bases:

>100_bases
AAAACCGACAGAGGACGCGCGCTTTTACGGCCGACGCAAGGGCAAGCCGCTTCGCGCCACTCGTCAGCGTCTTCTCGACA
CCATGCTGCCCGCCCTCACC

Product: s-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase 2; MAT 2; Methionine adenosyltransferase 2

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAQSEYLFTSESVSEGHPDKVCDRISDAIVDAFLAEDPHSRVALETMATTNFVVLAGEVRGPDSLTHDRLKEIAREAIKD
IGYEQRGFHWKDAEIVSHVHSQSADIAVGVDAAGNKDEGAGDQGIMFGYACTETEELMPAPIALSHAILKSLAEFRHGGD
TSFGPDSKSQVTLRYVDGKPVGAASVVVSTQHAGNLSQDEVRELVRPHVLKVLPEGWMCPEEEFYVNPTGRFVIGGPDGD
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAEKCVIQVSYAIGVSKPLSVYVNTQGTG
QVDEQRLAVVLQQLMDLSPRGIRQHLQLSRPIYARTAAYGHFGRKPEKDGGFSWERTDLVAGLKTAFGA

Sequences:

>Translated_389_residues
MAQSEYLFTSESVSEGHPDKVCDRISDAIVDAFLAEDPHSRVALETMATTNFVVLAGEVRGPDSLTHDRLKEIAREAIKD
IGYEQRGFHWKDAEIVSHVHSQSADIAVGVDAAGNKDEGAGDQGIMFGYACTETEELMPAPIALSHAILKSLAEFRHGGD
TSFGPDSKSQVTLRYVDGKPVGAASVVVSTQHAGNLSQDEVRELVRPHVLKVLPEGWMCPEEEFYVNPTGRFVIGGPDGD
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAEKCVIQVSYAIGVSKPLSVYVNTQGTG
QVDEQRLAVVLQQLMDLSPRGIRQHLQLSRPIYARTAAYGHFGRKPEKDGGFSWERTDLVAGLKTAFGA
>Mature_388_residues
AQSEYLFTSESVSEGHPDKVCDRISDAIVDAFLAEDPHSRVALETMATTNFVVLAGEVRGPDSLTHDRLKEIAREAIKDI
GYEQRGFHWKDAEIVSHVHSQSADIAVGVDAAGNKDEGAGDQGIMFGYACTETEELMPAPIALSHAILKSLAEFRHGGDT
SFGPDSKSQVTLRYVDGKPVGAASVVVSTQHAGNLSQDEVRELVRPHVLKVLPEGWMCPEEEFYVNPTGRFVIGGPDGDC
GLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAEKCVIQVSYAIGVSKPLSVYVNTQGTGQ
VDEQRLAVVLQQLMDLSPRGIRQHLQLSRPIYARTAAYGHFGRKPEKDGGFSWERTDLVAGLKTAFGA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=384, Percent_Identity=48.9583333333333, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI4557737, Length=384, Percent_Identity=47.3958333333333, Blast_Score=329, Evalue=3e-90,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=53.2133676092545, Blast_Score=403, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17538498, Length=385, Percent_Identity=49.8701298701299, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17538494, Length=385, Percent_Identity=49.3506493506493, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=49.3506493506493, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17551082, Length=382, Percent_Identity=47.9057591623037, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565905, Length=383, Percent_Identity=46.2140992167102, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32565911, Length=334, Percent_Identity=47.3053892215569, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25145635, Length=334, Percent_Identity=47.0059880239521, Blast_Score=261, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=48.8294314381271, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI32565909, Length=100, Percent_Identity=48, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32565907, Length=43, Percent_Identity=72.0930232558139, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=380, Percent_Identity=47.1052631578947, Blast_Score=324, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=46.6321243523316, Blast_Score=319, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24580527, Length=379, Percent_Identity=48.5488126649077, Blast_Score=321, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24580525, Length=379, Percent_Identity=48.5488126649077, Blast_Score=321, Evalue=5e-88,
Organism=Drosophila melanogaster, GI45552159, Length=379, Percent_Identity=48.2849604221636, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580529, Length=379, Percent_Identity=48.2849604221636, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580537, Length=379, Percent_Identity=48.2849604221636, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580533, Length=379, Percent_Identity=48.2849604221636, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580535, Length=379, Percent_Identity=48.2849604221636, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580531, Length=379, Percent_Identity=48.2849604221636, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580541, Length=380, Percent_Identity=46.3157894736842, Blast_Score=298, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=45.4545454545455, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK2_RHORT (Q2RMS5)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428857.1
- ProteinModelPortal:   Q2RMS5
- SMR:   Q2RMS5
- STRING:   Q2RMS5
- GeneID:   3837233
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3776
- NMPDR:   fig|1085.1.peg.1139
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   PRK05250
- BioCyc:   RRUB269796:RRU_A3776-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41753; Mature: 41622

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSEYLFTSESVSEGHPDKVCDRISDAIVDAFLAEDPHSRVALETMATTNFVVLAGEVR
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHCCCCEEEEEECCC
GPDSLTHDRLKEIAREAIKDIGYEQRGFHWKDAEIVSHVHSQSADIAVGVDAAGNKDEGA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCC
GDQGIMFGYACTETEELMPAPIALSHAILKSLAEFRHGGDTSFGPDSKSQVTLRYVDGKP
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCC
VGAASVVVSTQHAGNLSQDEVRELVRPHVLKVLPEGWMCPEEEFYVNPTGRFVIGGPDGD
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECCCCC
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAEKCVIQV
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
SYAIGVSKPLSVYVNTQGTGQVDEQRLAVVLQQLMDLSPRGIRQHLQLSRPIYARTAAYG
EHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
HFGRKPEKDGGFSWERTDLVAGLKTAFGA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQSEYLFTSESVSEGHPDKVCDRISDAIVDAFLAEDPHSRVALETMATTNFVVLAGEVR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHCCCCEEEEEECCC
GPDSLTHDRLKEIAREAIKDIGYEQRGFHWKDAEIVSHVHSQSADIAVGVDAAGNKDEGA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCC
GDQGIMFGYACTETEELMPAPIALSHAILKSLAEFRHGGDTSFGPDSKSQVTLRYVDGKP
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCC
VGAASVVVSTQHAGNLSQDEVRELVRPHVLKVLPEGWMCPEEEFYVNPTGRFVIGGPDGD
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECCCCC
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAEKCVIQV
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
SYAIGVSKPLSVYVNTQGTGQVDEQRLAVVLQQLMDLSPRGIRQHLQLSRPIYARTAAYG
EHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
HFGRKPEKDGGFSWERTDLVAGLKTAFGA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA