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The map label for this gene is miaB

Identifier: 83595099

GI number: 83595099

Start: 4318290

End: 4319693

Strand: Direct

Name: miaB

Synonym: Rru_A3770

Alternate gene names: 83595099

Gene position: 4318290-4319693 (Clockwise)

Preceding gene: 83595098

Following gene: 83595100

Centisome position: 99.21

GC content: 66.17

Gene sequence:

>1404_bases
TTGGCGAACAGAAAACTCTTCATAAAGTCTTACGGCTGTCAGATGAATGTTTATGATGCCGGCCGCATGGCCGATGTCAT
GGCGCCGCTGGGCTATGATCTCGTTTCGGAGGCCGATGGCGCCGATCTGGTGATCCTCAATACCTGCCATATCCGCGAAA
AAGCCGCCGAGAAGGTGTTTTCCGATCTTGGCCGCCTGCGTCCCTTCAAGGAGGCGATGGCCGCCGAAGGCCGGCGCATG
CTGGTCGCCGTCGCCGGATGCGTCGCCCAGGCCGAAGGCGCTGAATTGATGGCGCGGGCCAAGATCGTCGATATGGTCGT
CGGGCCCCAGGCCTATCACCGCCTGCCCGAAATGGTCGCCAAGGTCGAACGCGGCGCTGGCCGGGTGCTCGATACCGATT
TCCCGATCGAGCCGAAGTTCGATTTCCTGCCCGCGCCCCAGGCCCATGGCCCCTCGGCCTTCCTGTCGGTGCAAGAGGGC
TGCGACAAGTTCTGCACCTTCTGCGTCGTGCCCTATACGCGCGGCGCCGAATATTCCCGCCCGGTGGCCGATGTCCTGCG
CGAAGCCGCCTTGCTGGCCGAAGGCGGGGTGCGCGAAATCACCCTGCTTGGCCAGAACGTCAACGCCTATCACGGCGAAG
CCCCCGATGGCCGGACCTGGGGGCTGGGCCGGCTGGCCCGCGCCCTGGCCGAGATTCCCGGCGTCGCCCGTTTGCGCTAC
ACCACCAGCCATCCCCGCGATGTCGATGACGATCTGATCGCCGCCCACCGCGAGGTCGCGGCCTTGACGCCGTTTATCCA
CCTGCCCGTTCAGTCCGGTTCGGACCGTATTCTGGCGGCGATGAACCGCGGCCATGATGTTGAGGGCTATCGGCGGATCA
TCGACCGGCTGCGCGCCGCCCGCGATGACATGGCCTTCTCCTCGGATTTCATCGTCGGCTTCCCCGGCGAGAGCGAGCGG
GATTTCCAGGCCACCCTCGCCCTGATCGACGAGGTGCGCTTCATTCAGGCTTATTCGTTCAAATATAGCCCGCGACCGGG
AACCCCGGCCGCCGCCCTGTCCGCGCAACTGCCCGAGGCCGAGAAATCGGCGCGGCTGATCGCCTTGCAGGCCCGGCTGG
TCGAGATCCAGCAGGCGTTCAATCAGGCTTGCGTCGGCCGGCCGATGGACGTTCTCCTCGATCGTCCCGGCCGCCATGCC
GGTCAGCTGGTCGGCCGCAGCCCGTGGATGCAGCCCGTGCATCTCGAGGCGCCGGCGGCGCTGCTTGGCACCCTTGCCCC
CGTGGTCGTCGAGACGGCCACGTCCAACAGTCTGGCGGCGCGCCTTGTGGAAGGGAACTCATCCATGTCCTCCGATCGGG
TTCTTGCAGAGGAAGCGCCGCCGCCCGCGCGCATCCGGGCGTGA

Upstream 100 bases:

>100_bases
GGTCAAAACGGTCGCGCCGCCGGCGGGCCCTTGACTCGCGGTCCGGCGATGGTAGCTTGATTTTTTAGACTTCCACGGAT
GGCCCTCGGGCCGGAAAGCC

Downstream 100 bases:

>100_bases
GCGACGAAGAAACCCGCAGCACCCGGTCCTATGACGATAACGGGGCCCTGCAGATCCTGATCGGCGCCGCCGGCGACAAT
CTCGATCGTCTTGAACAGGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MANRKLFIKSYGCQMNVYDAGRMADVMAPLGYDLVSEADGADLVILNTCHIREKAAEKVFSDLGRLRPFKEAMAAEGRRM
LVAVAGCVAQAEGAELMARAKIVDMVVGPQAYHRLPEMVAKVERGAGRVLDTDFPIEPKFDFLPAPQAHGPSAFLSVQEG
CDKFCTFCVVPYTRGAEYSRPVADVLREAALLAEGGVREITLLGQNVNAYHGEAPDGRTWGLGRLARALAEIPGVARLRY
TTSHPRDVDDDLIAAHREVAALTPFIHLPVQSGSDRILAAMNRGHDVEGYRRIIDRLRAARDDMAFSSDFIVGFPGESER
DFQATLALIDEVRFIQAYSFKYSPRPGTPAAALSAQLPEAEKSARLIALQARLVEIQQAFNQACVGRPMDVLLDRPGRHA
GQLVGRSPWMQPVHLEAPAALLGTLAPVVVETATSNSLAARLVEGNSSMSSDRVLAEEAPPPARIRA

Sequences:

>Translated_467_residues
MANRKLFIKSYGCQMNVYDAGRMADVMAPLGYDLVSEADGADLVILNTCHIREKAAEKVFSDLGRLRPFKEAMAAEGRRM
LVAVAGCVAQAEGAELMARAKIVDMVVGPQAYHRLPEMVAKVERGAGRVLDTDFPIEPKFDFLPAPQAHGPSAFLSVQEG
CDKFCTFCVVPYTRGAEYSRPVADVLREAALLAEGGVREITLLGQNVNAYHGEAPDGRTWGLGRLARALAEIPGVARLRY
TTSHPRDVDDDLIAAHREVAALTPFIHLPVQSGSDRILAAMNRGHDVEGYRRIIDRLRAARDDMAFSSDFIVGFPGESER
DFQATLALIDEVRFIQAYSFKYSPRPGTPAAALSAQLPEAEKSARLIALQARLVEIQQAFNQACVGRPMDVLLDRPGRHA
GQLVGRSPWMQPVHLEAPAALLGTLAPVVVETATSNSLAARLVEGNSSMSSDRVLAEEAPPPARIRA
>Mature_466_residues
ANRKLFIKSYGCQMNVYDAGRMADVMAPLGYDLVSEADGADLVILNTCHIREKAAEKVFSDLGRLRPFKEAMAAEGRRML
VAVAGCVAQAEGAELMARAKIVDMVVGPQAYHRLPEMVAKVERGAGRVLDTDFPIEPKFDFLPAPQAHGPSAFLSVQEGC
DKFCTFCVVPYTRGAEYSRPVADVLREAALLAEGGVREITLLGQNVNAYHGEAPDGRTWGLGRLARALAEIPGVARLRYT
TSHPRDVDDDLIAAHREVAALTPFIHLPVQSGSDRILAAMNRGHDVEGYRRIIDRLRAARDDMAFSSDFIVGFPGESERD
FQATLALIDEVRFIQAYSFKYSPRPGTPAAALSAQLPEAEKSARLIALQARLVEIQQAFNQACVGRPMDVLLDRPGRHAG
QLVGRSPWMQPVHLEAPAALLGTLAPVVVETATSNSLAARLVEGNSSMSSDRVLAEEAPPPARIRA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=431, Percent_Identity=35.7308584686775, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI28872782, Length=432, Percent_Identity=35.6481481481481, Blast_Score=254, Evalue=9e-68,
Organism=Homo sapiens, GI93277076, Length=438, Percent_Identity=28.0821917808219, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=47.4387527839644, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1787057, Length=424, Percent_Identity=31.3679245283019, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=476, Percent_Identity=31.9327731092437, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=281, Percent_Identity=30.9608540925267, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356207, Length=370, Percent_Identity=33.7837837837838, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=27.9475982532751, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_RHORT (Q2RMT1)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428851.1
- ProteinModelPortal:   Q2RMT1
- SMR:   Q2RMT1
- STRING:   Q2RMT1
- GeneID:   3837227
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3770
- NMPDR:   fig|1085.1.peg.1145
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q2RMT1
- ProtClustDB:   PRK14325
- BioCyc:   RRUB269796:RRU_A3770-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50720; Mature: 50588

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRKLFIKSYGCQMNVYDAGRMADVMAPLGYDLVSEADGADLVILNTCHIREKAAEKVF
CCCCEEEEEECCCEEEECCCCHHHHHHHHCCCHHHHCCCCCCEEEEECCHHHHHHHHHHH
SDLGRLRPFKEAMAAEGRRMLVAVAGCVAQAEGAELMARAKIVDMVVGPQAYHRLPEMVA
HHHHHCCHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
KVERGAGRVLDTDFPIEPKFDFLPAPQAHGPSAFLSVQEGCDKFCTFCVVPYTRGAEYSR
HHHHCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PVADVLREAALLAEGGVREITLLGQNVNAYHGEAPDGRTWGLGRLARALAEIPGVARLRY
CHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
TTSHPRDVDDDLIAAHREVAALTPFIHLPVQSGSDRILAAMNRGHDVEGYRRIIDRLRAA
ECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
RDDMAFSSDFIVGFPGESERDFQATLALIDEVRFIQAYSFKYSPRPGTPAAALSAQLPEA
HHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHCCCCH
EKSARLIALQARLVEIQQAFNQACVGRPMDVLLDRPGRHAGQLVGRSPWMQPVHLEAPAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHCCCCCCCCCEECCCHHH
LLGTLAPVVVETATSNSLAARLVEGNSSMSSDRVLAEEAPPPARIRA
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
ANRKLFIKSYGCQMNVYDAGRMADVMAPLGYDLVSEADGADLVILNTCHIREKAAEKVF
CCCEEEEEECCCEEEECCCCHHHHHHHHCCCHHHHCCCCCCEEEEECCHHHHHHHHHHH
SDLGRLRPFKEAMAAEGRRMLVAVAGCVAQAEGAELMARAKIVDMVVGPQAYHRLPEMVA
HHHHHCCHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
KVERGAGRVLDTDFPIEPKFDFLPAPQAHGPSAFLSVQEGCDKFCTFCVVPYTRGAEYSR
HHHHCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PVADVLREAALLAEGGVREITLLGQNVNAYHGEAPDGRTWGLGRLARALAEIPGVARLRY
CHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
TTSHPRDVDDDLIAAHREVAALTPFIHLPVQSGSDRILAAMNRGHDVEGYRRIIDRLRAA
ECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
RDDMAFSSDFIVGFPGESERDFQATLALIDEVRFIQAYSFKYSPRPGTPAAALSAQLPEA
HHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHCCCCH
EKSARLIALQARLVEIQQAFNQACVGRPMDVLLDRPGRHAGQLVGRSPWMQPVHLEAPAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHCCCCCCCCCEECCCHHH
LLGTLAPVVVETATSNSLAARLVEGNSSMSSDRVLAEEAPPPARIRA
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA