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The map label for this gene is argG [H]

Identifier: 83595080

GI number: 83595080

Start: 4299276

End: 4300535

Strand: Direct

Name: argG [H]

Synonym: Rru_A3751

Alternate gene names: 83595080

Gene position: 4299276-4300535 (Clockwise)

Preceding gene: 83595079

Following gene: 83595081

Centisome position: 98.77

GC content: 62.54

Gene sequence:

>1260_bases
TTGGGTTCCGGCTGCATTCCTCCCATGGTCAAGAAGGCGCTTTCCATGAAGAAAGGTGACGTGAAGAAGGTTGTTCTCGC
CTATTCCGGCGGTTTGGACACCTCGATCATCTTGCGTTGGCTGCAAGACGAATACGACTGCGAGGTGGTGACCTTCACCG
CCGATATCGGTCAGGGCGAGGAACTGGAGCCGGCGCGCCAGAAGGCCGAGATGATGGGGATCAAGGAGATCTACATCGAG
GACCTGCGCGAGGAATTCGTCCGCGACTACGTTTTCCCGATGTTCCGCGCCAATACCCTTTATGAAGGCGTCTATCTGCT
GGGCACCTCGATCGCCCGGCCGCTGATCGGCAAGCGTCTGGTCGAGATCGCCGAGGCGACCGGCGCCGACGCCGTCAGCC
ACGGCGCGACCGGCAAGGGCAACGATCAGGTGCGGTTCGAGCTGACCGCCTATGCGCTCAAGCCCGACATCAAGATCATC
GCGCCTTGGCGCACCTGGGATCTGCACAGCCGCACCAAGCTGATCGAATACGCCATGCGCCACCAGATCCCCGTGCCCAA
GGACAAGCACGGCGAGGCGCCCTATTCGATGGACGCCAATCTTCTGCACATCTCCTATGAAGGCAAGGCCCTGGAGAACC
CCTGGACCGAGCCCAGCGAGGACATGTTCCGCCTGACGGTCAGCCCGGAAGCCGCCCCCGACAAGGCCCAGTACATCGAG
GTCGATTTCGAGCGCGGCGATGCCGTGGCCATCGATGGCGAAAAGCTGACCCCGGCCGCCCTGCTCGCCAAGCTCAACGA
GATCGGCGGCCGCCATGGCGTGGGCCGTCTGGATCTGGTCGAAAACCGCTATGTCGGCATGAAGTCGCGCGGCGTTTATG
AAACCCCGGGCGGCACCATCTTGCAGGTCGCCCACCGCGCCGTGGAAAGCCTGACCCTTGATCGCGAGGTCATGCACCTG
CGCGACGAGCTGATGCCGCGCTATGCCAAGCTGATCTACAACGGCTTCTGGTTCGCCCCCGAGCGCCTGATGCTGCAGGC
CGCCATCGATCAGACCCAGCAGACGGTGACCGGCACCGCCCGCCTGAAGCTCTACAAGGGCAATGTCAGCGTCGTCGGCC
GCAAGGCGGCGAAAAGCCTCTATCGCATGGACTATGTCACCTTCGAGGAAGACACCGTCTATGACCAGCACGACGCCGAA
GGCTTCATCAAACTGAACGCCCTGCGTCTGCGTCTGGGCAAGATGGCCCGCGACAGCTGA

Upstream 100 bases:

>100_bases
TCAAGACCAAAATGGATCACCCCAAAAGGGACCCCCGATGACCTTGTCCGGCGCGTTCCTCGGTTTTTTGCTTTTTCCCT
TTGTACCCGGGACCTTATGA

Downstream 100 bases:

>100_bases
TCGCGGTCGCTGCGTGCCAGACTTAAGCAAAGGCCCCTCTCGCGAGGGGCCTTTCGTCTGACCGGGTTCACAGGGCGCCC
CGCCTGATGTAAATTAGACC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MGSGCIPPMVKKALSMKKGDVKKVVLAYSGGLDTSIILRWLQDEYDCEVVTFTADIGQGEELEPARQKAEMMGIKEIYIE
DLREEFVRDYVFPMFRANTLYEGVYLLGTSIARPLIGKRLVEIAEATGADAVSHGATGKGNDQVRFELTAYALKPDIKII
APWRTWDLHSRTKLIEYAMRHQIPVPKDKHGEAPYSMDANLLHISYEGKALENPWTEPSEDMFRLTVSPEAAPDKAQYIE
VDFERGDAVAIDGEKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTILQVAHRAVESLTLDREVMHL
RDELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTARLKLYKGNVSVVGRKAAKSLYRMDYVTFEEDTVYDQHDAE
GFIKLNALRLRLGKMARDS

Sequences:

>Translated_419_residues
MGSGCIPPMVKKALSMKKGDVKKVVLAYSGGLDTSIILRWLQDEYDCEVVTFTADIGQGEELEPARQKAEMMGIKEIYIE
DLREEFVRDYVFPMFRANTLYEGVYLLGTSIARPLIGKRLVEIAEATGADAVSHGATGKGNDQVRFELTAYALKPDIKII
APWRTWDLHSRTKLIEYAMRHQIPVPKDKHGEAPYSMDANLLHISYEGKALENPWTEPSEDMFRLTVSPEAAPDKAQYIE
VDFERGDAVAIDGEKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTILQVAHRAVESLTLDREVMHL
RDELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTARLKLYKGNVSVVGRKAAKSLYRMDYVTFEEDTVYDQHDAE
GFIKLNALRLRLGKMARDS
>Mature_418_residues
GSGCIPPMVKKALSMKKGDVKKVVLAYSGGLDTSIILRWLQDEYDCEVVTFTADIGQGEELEPARQKAEMMGIKEIYIED
LREEFVRDYVFPMFRANTLYEGVYLLGTSIARPLIGKRLVEIAEATGADAVSHGATGKGNDQVRFELTAYALKPDIKIIA
PWRTWDLHSRTKLIEYAMRHQIPVPKDKHGEAPYSMDANLLHISYEGKALENPWTEPSEDMFRLTVSPEAAPDKAQYIEV
DFERGDAVAIDGEKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTILQVAHRAVESLTLDREVMHLR
DELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTARLKLYKGNVSVVGRKAAKSLYRMDYVTFEEDTVYDQHDAEG
FIKLNALRLRLGKMARDS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=47.2361809045226, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=47.2361809045226, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1789563, Length=405, Percent_Identity=27.1604938271605, Blast_Score=138, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=411, Percent_Identity=45.0121654501217, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21358151, Length=401, Percent_Identity=46.3840399002494, Blast_Score=348, Evalue=3e-96,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 47111; Mature: 46979

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSGCIPPMVKKALSMKKGDVKKVVLAYSGGLDTSIILRWLQDEYDCEVVTFTADIGQGE
CCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCC
ELEPARQKAEMMGIKEIYIEDLREEFVRDYVFPMFRANTLYEGVYLLGTSIARPLIGKRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEIAEATGADAVSHGATGKGNDQVRFELTAYALKPDIKIIAPWRTWDLHSRTKLIEYAMR
HHHHHHCCCCHHHCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHH
HQIPVPKDKHGEAPYSMDANLLHISYEGKALENPWTEPSEDMFRLTVSPEAAPDKAQYIE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEE
VDFERGDAVAIDGEKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTI
EEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECCCCHH
LQVAHRAVESLTLDREVMHLRDELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHEEEE
RLKLYKGNVSVVGRKAAKSLYRMDYVTFEEDTVYDQHDAEGFIKLNALRLRLGKMARDS
EEEEEECCCEEEEHHHHHHHHHHHCEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
GSGCIPPMVKKALSMKKGDVKKVVLAYSGGLDTSIILRWLQDEYDCEVVTFTADIGQGE
CCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCC
ELEPARQKAEMMGIKEIYIEDLREEFVRDYVFPMFRANTLYEGVYLLGTSIARPLIGKRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEIAEATGADAVSHGATGKGNDQVRFELTAYALKPDIKIIAPWRTWDLHSRTKLIEYAMR
HHHHHHCCCCHHHCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHH
HQIPVPKDKHGEAPYSMDANLLHISYEGKALENPWTEPSEDMFRLTVSPEAAPDKAQYIE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEE
VDFERGDAVAIDGEKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTI
EEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECCCCHH
LQVAHRAVESLTLDREVMHLRDELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHEEEE
RLKLYKGNVSVVGRKAAKSLYRMDYVTFEEDTVYDQHDAEGFIKLNALRLRLGKMARDS
EEEEEECCCEEEEHHHHHHHHHHHCEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA