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The map label for this gene is acsA1 [H]

Identifier: 83595024

GI number: 83595024

Start: 4240734

End: 4242740

Strand: Direct

Name: acsA1 [H]

Synonym: Rru_A3695

Alternate gene names: 83595024

Gene position: 4240734-4242740 (Clockwise)

Preceding gene: 83595023

Following gene: 83595031

Centisome position: 97.42

GC content: 66.42

Gene sequence:

>2007_bases
GTGACAGAAAGCGAGCAGGCGGTGATTTCGGGTTTTGCCAAGGGCGAGCCGGCGCCGCCGCTGTGGACTCCCTCGGCCGA
GCGCATCGCCGCCACCCGGCTTGACGCCTTCCGCCGCCGCATCGAAGAGCGCCACGGCGTCGTCCTGGCCGATTACGATG
CCCTGTGGCAATGGTCGATCGATGCCCCGGCCGCCTTCTGGACCGAGGTCTGGGATTTTTGCGGGGTCATCGGCGATAAG
GGCGACGGGCCGGTGGTCGATGATCCGGGCAAGCTTCCCGGCGCGCGCTGGTTCGCCGGCGCCCGGCTCAACGCCGCCGA
AACCCTGCTCGCCCCCCATGCCCAGCGCCCGGGCGACGCCGTGGTCTTCCGCGTCGAGAACCATCCGACCCAACGCCTTG
ATCTGCCGACGCTGCGCGCCCGGGTGTCGCTGGTTCAACAAGGCTTGCGCCGCGCTGGCGTCGGGGTTGGCGACCGGGTG
GCGGGCTATCTGCCCAATATGCCCGAGGCCCTGATCGCCATGCTGGCGACCGCCGGCCTGGGGGCGGTGTGGTCTTCGGC
CTCGCCGGATTTCGGCGCCCAGGGGGTGATCGACCGCTTCGGCCAGATCGAGCCCAAGGTGCTGTTCACGGTCGATGGCT
ATTTTTACAACGGCAAGGTCCATGACTGCCGCGAGAAGGTCGCCACCCTGGTCAAGGCGGTGCCGTCGATCACCCAGGTG
GTGGAAGTGGCCTTCGCCGGCAAGGGCGATGCGCCCGCTGCCATCCCCCGCGCCACGTCATGGGCGGCGTTCATCGGCGA
TTTCACCGCCGGCGAGCCGGACTTCCTGCGGCTGCCCTTCGATCATCCGCTGTATATCCTGTATTCCAGCGGCACCACCG
GCGTGCCAAAATGCATCGTTCACGGCCATGGCGGCACCTTGCTTCAGCAACTGAAGGAGCATGTGCTGCATGCCGATGCC
CACCCCGGCGACCGGGTGTTCTATTTCACCACCTGCGGCTGGATGATGTGGAACTGGCTGGCCGCGGCCTTGGGGGCGGG
CTGCACCGTGCTGCTTTATGACGGATCGCCGTTTTATCCCGATGGCGCCGTGCTGTGGGATTACGCCCAGGCCGAGGGCT
GCACCTTCTTTGGCACCTCGGCCAAATGGATCGACGCCCAGCGCAAGGCCAGGTTGCGGCCTAGCGATACCCATGATCTG
TCGACGGTGCGGGTGATCGCCTCCACCGGCTCGCCGCTCGCCCCGGAAAGCTTCGATTACGTCTACGACGCCATCAAAGG
CGATGTGCAACTGGCCTCGATCTCGGGCGGCACCGATATCTTGTCGTGTTTCATGCTGGGCAGCCCAATTGATTCGGTGT
TTCGCGGCGAGATCCAGAAGCGCGGCCTTGGCATGGCGGTGGCGGTTTTCGACGACGAGGGCCGGCCGCTGGTCGGCCAG
AAGGGCGAACTGGTCTGCACCCGGGCCTTTCCCTCCAGCCCCATTGGGTTCTGGAACGATGCCGACGGGGCGAAGTTCAA
GGCCGCCTATTTCGAGCGCTTCCCCGGGGTCTGGACCCATGGCGACTGGGTGGAATTGACCCCCCACGGCGGCATCGTCG
TTTATGGCCGCTCGGATGCGACGCTCAATCCCGGCGGCGTGCGCATCGGCACCGCCGAGATCTATCGGCAGGTTGAAACC
CTTGACGAGGTGCGCGAGGCCATCGTCATCGGCCAGGACTGGCAAGGCGATGTGCGGGTGGTGCTGTTCGTCGTGCTGCG
CGACGGACTGACGCTGACGCCCGAGCTTGAGGACAAAATCAAGGCGCGGATCCGTCAATCCTGCACGCCGCGCCACGTGC
CGGCCAAGGTGGTTCAGGTCAACGATATTCCCCGCACCAAATCCAACAAGATCGTGGAACTGGCGGTGCGCGAGGTCGTC
CACGGCCGGCCGGTGCGCAACCGCGAGGCCCTGGCCAACCCCGAGGCGCTCGACGACTTCGCCGGACGCCCCGAACTGGC
GGGATAG

Upstream 100 bases:

>100_bases
TAAAACCCCAGGCTGTATTGCATTCACGACGGGGCGATACAATCTCCCTGACGGGGATGGCGCGGGTCGGGCCGCCCCCC
ACCGTCAGGGAGGAGCGAAC

Downstream 100 bases:

>100_bases
GGCTTAGCCCTTTGTTGGCGGGTCGATATGCACGATCACCGGCACATGGTCCGAGGGCGGGGTCCAGTCGCGGGTGTCGT
CCATCACCTCCAGGCCGCGC

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MTESEQAVISGFAKGEPAPPLWTPSAERIAATRLDAFRRRIEERHGVVLADYDALWQWSIDAPAAFWTEVWDFCGVIGDK
GDGPVVDDPGKLPGARWFAGARLNAAETLLAPHAQRPGDAVVFRVENHPTQRLDLPTLRARVSLVQQGLRRAGVGVGDRV
AGYLPNMPEALIAMLATAGLGAVWSSASPDFGAQGVIDRFGQIEPKVLFTVDGYFYNGKVHDCREKVATLVKAVPSITQV
VEVAFAGKGDAPAAIPRATSWAAFIGDFTAGEPDFLRLPFDHPLYILYSSGTTGVPKCIVHGHGGTLLQQLKEHVLHADA
HPGDRVFYFTTCGWMMWNWLAAALGAGCTVLLYDGSPFYPDGAVLWDYAQAEGCTFFGTSAKWIDAQRKARLRPSDTHDL
STVRVIASTGSPLAPESFDYVYDAIKGDVQLASISGGTDILSCFMLGSPIDSVFRGEIQKRGLGMAVAVFDDEGRPLVGQ
KGELVCTRAFPSSPIGFWNDADGAKFKAAYFERFPGVWTHGDWVELTPHGGIVVYGRSDATLNPGGVRIGTAEIYRQVET
LDEVREAIVIGQDWQGDVRVVLFVVLRDGLTLTPELEDKIKARIRQSCTPRHVPAKVVQVNDIPRTKSNKIVELAVREVV
HGRPVRNREALANPEALDDFAGRPELAG

Sequences:

>Translated_668_residues
MTESEQAVISGFAKGEPAPPLWTPSAERIAATRLDAFRRRIEERHGVVLADYDALWQWSIDAPAAFWTEVWDFCGVIGDK
GDGPVVDDPGKLPGARWFAGARLNAAETLLAPHAQRPGDAVVFRVENHPTQRLDLPTLRARVSLVQQGLRRAGVGVGDRV
AGYLPNMPEALIAMLATAGLGAVWSSASPDFGAQGVIDRFGQIEPKVLFTVDGYFYNGKVHDCREKVATLVKAVPSITQV
VEVAFAGKGDAPAAIPRATSWAAFIGDFTAGEPDFLRLPFDHPLYILYSSGTTGVPKCIVHGHGGTLLQQLKEHVLHADA
HPGDRVFYFTTCGWMMWNWLAAALGAGCTVLLYDGSPFYPDGAVLWDYAQAEGCTFFGTSAKWIDAQRKARLRPSDTHDL
STVRVIASTGSPLAPESFDYVYDAIKGDVQLASISGGTDILSCFMLGSPIDSVFRGEIQKRGLGMAVAVFDDEGRPLVGQ
KGELVCTRAFPSSPIGFWNDADGAKFKAAYFERFPGVWTHGDWVELTPHGGIVVYGRSDATLNPGGVRIGTAEIYRQVET
LDEVREAIVIGQDWQGDVRVVLFVVLRDGLTLTPELEDKIKARIRQSCTPRHVPAKVVQVNDIPRTKSNKIVELAVREVV
HGRPVRNREALANPEALDDFAGRPELAG
>Mature_667_residues
TESEQAVISGFAKGEPAPPLWTPSAERIAATRLDAFRRRIEERHGVVLADYDALWQWSIDAPAAFWTEVWDFCGVIGDKG
DGPVVDDPGKLPGARWFAGARLNAAETLLAPHAQRPGDAVVFRVENHPTQRLDLPTLRARVSLVQQGLRRAGVGVGDRVA
GYLPNMPEALIAMLATAGLGAVWSSASPDFGAQGVIDRFGQIEPKVLFTVDGYFYNGKVHDCREKVATLVKAVPSITQVV
EVAFAGKGDAPAAIPRATSWAAFIGDFTAGEPDFLRLPFDHPLYILYSSGTTGVPKCIVHGHGGTLLQQLKEHVLHADAH
PGDRVFYFTTCGWMMWNWLAAALGAGCTVLLYDGSPFYPDGAVLWDYAQAEGCTFFGTSAKWIDAQRKARLRPSDTHDLS
TVRVIASTGSPLAPESFDYVYDAIKGDVQLASISGGTDILSCFMLGSPIDSVFRGEIQKRGLGMAVAVFDDEGRPLVGQK
GELVCTRAFPSSPIGFWNDADGAKFKAAYFERFPGVWTHGDWVELTPHGGIVVYGRSDATLNPGGVRIGTAEIYRQVETL
DEVREAIVIGQDWQGDVRVVLFVVLRDGLTLTPELEDKIKARIRQSCTPRHVPAKVVQVNDIPRTKSNKIVELAVREVVH
GRPVRNREALANPEALDDFAGRPELAG

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=660, Percent_Identity=42.4242424242424, Blast_Score=568, Evalue=1e-162,
Organism=Homo sapiens, GI8923896, Length=683, Percent_Identity=25.7686676427526, Blast_Score=173, Evalue=5e-43,
Organism=Homo sapiens, GI28416953, Length=618, Percent_Identity=26.3754045307443, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI253970489, Length=696, Percent_Identity=25.2873563218391, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI13375727, Length=583, Percent_Identity=24.6998284734134, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1790505, Length=625, Percent_Identity=26.56, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1786529, Length=634, Percent_Identity=24.1324921135647, Blast_Score=123, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17569507, Length=655, Percent_Identity=37.4045801526718, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71983001, Length=652, Percent_Identity=25.7668711656442, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71982997, Length=652, Percent_Identity=25.7668711656442, Blast_Score=142, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=641, Percent_Identity=26.3650546021841, Blast_Score=183, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6323182, Length=656, Percent_Identity=26.0670731707317, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24667955, Length=661, Percent_Identity=25.4160363086233, Blast_Score=176, Evalue=6e-44,
Organism=Drosophila melanogaster, GI62472339, Length=574, Percent_Identity=25.7839721254355, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=25.1417769376182, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21355225, Length=642, Percent_Identity=24.6105919003115, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 72593; Mature: 72462

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESEQAVISGFAKGEPAPPLWTPSAERIAATRLDAFRRRIEERHGVVLADYDALWQWSI
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHEEEECC
DAPAAFWTEVWDFCGVIGDKGDGPVVDDPGKLPGARWFAGARLNAAETLLAPHAQRPGDA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCE
VVFRVENHPTQRLDLPTLRARVSLVQQGLRRAGVGVGDRVAGYLPNMPEALIAMLATAGL
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHCC
GAVWSSASPDFGAQGVIDRFGQIEPKVLFTVDGYFYNGKVHDCREKVATLVKAVPSITQV
HHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHH
VEVAFAGKGDAPAAIPRATSWAAFIGDFTAGEPDFLRLPFDHPLYILYSSGTTGVPKCIV
HHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCCCCEEEEECCCCCCCCEEEE
HGHGGTLLQQLKEHVLHADAHPGDRVFYFTTCGWMMWNWLAAALGAGCTVLLYDGSPFYP
ECCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCC
DGAVLWDYAQAEGCTFFGTSAKWIDAQRKARLRPSDTHDLSTVRVIASTGSPLAPESFDY
CCCEEEEECCCCCCEEECCCCHHHCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHH
VYDAIKGDVQLASISGGTDILSCFMLGSPIDSVFRGEIQKRGLGMAVAVFDDEGRPLVGQ
HHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
KGELVCTRAFPSSPIGFWNDADGAKFKAAYFERFPGVWTHGDWVELTPHGGIVVYGRSDA
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCC
TLNPGGVRIGTAEIYRQVETLDEVREAIVIGQDWQGDVRVVLFVVLRDGLTLTPELEDKI
EECCCCEEECHHHHHHHHHHHHHHHHHEEECCCCCCCHHHEEEEEHHCCCEECCCHHHHH
KARIRQSCTPRHVPAKVVQVNDIPRTKSNKIVELAVREVVHGRPVRNREALANPEALDDF
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
AGRPELAG
CCCCCCCC
>Mature Secondary Structure 
TESEQAVISGFAKGEPAPPLWTPSAERIAATRLDAFRRRIEERHGVVLADYDALWQWSI
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHEEEECC
DAPAAFWTEVWDFCGVIGDKGDGPVVDDPGKLPGARWFAGARLNAAETLLAPHAQRPGDA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCE
VVFRVENHPTQRLDLPTLRARVSLVQQGLRRAGVGVGDRVAGYLPNMPEALIAMLATAGL
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHCC
GAVWSSASPDFGAQGVIDRFGQIEPKVLFTVDGYFYNGKVHDCREKVATLVKAVPSITQV
HHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHH
VEVAFAGKGDAPAAIPRATSWAAFIGDFTAGEPDFLRLPFDHPLYILYSSGTTGVPKCIV
HHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCCCCEEEEECCCCCCCCEEEE
HGHGGTLLQQLKEHVLHADAHPGDRVFYFTTCGWMMWNWLAAALGAGCTVLLYDGSPFYP
ECCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCC
DGAVLWDYAQAEGCTFFGTSAKWIDAQRKARLRPSDTHDLSTVRVIASTGSPLAPESFDY
CCCEEEEECCCCCCEEECCCCHHHCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHH
VYDAIKGDVQLASISGGTDILSCFMLGSPIDSVFRGEIQKRGLGMAVAVFDDEGRPLVGQ
HHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
KGELVCTRAFPSSPIGFWNDADGAKFKAAYFERFPGVWTHGDWVELTPHGGIVVYGRSDA
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCC
TLNPGGVRIGTAEIYRQVETLDEVREAIVIGQDWQGDVRVVLFVVLRDGLTLTPELEDKI
EECCCCEEECHHHHHHHHHHHHHHHHHEEECCCCCCCHHHEEEEEHHCCCEECCCHHHHH
KARIRQSCTPRHVPAKVVQVNDIPRTKSNKIVELAVREVVHGRPVRNREALANPEALDDF
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
AGRPELAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]