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The map label for this gene is rsmB [H]

Identifier: 83594989

GI number: 83594989

Start: 4201854

End: 4203170

Strand: Reverse

Name: rsmB [H]

Synonym: Rru_A3660

Alternate gene names: 83594989

Gene position: 4203170-4201854 (Counterclockwise)

Preceding gene: 83594990

Following gene: 83594988

Centisome position: 96.56

GC content: 73.04

Gene sequence:

>1317_bases
ATGAAGGATACCGCCACCGCCACGCGGGCCGCCGCGCTCGACCTCCTCTCGGCCGTTCTCGATCACGGCCGGCCCCTGGA
CGACGTTTTCGACGCGGCGACGCGCAAGCTGGAGCCGCGCGACCGGGCCTTCGCCCGCCTGCTCGCCGCCACCACGCTGC
GACGGATGGGTCGCCTGGAAAAGGTCATCAAGACTTTCCTCGACCGGCCGCTGCCGCGCCGGGCGGTCGGCGCCAAGCAT
ATCCTGCGCCTGGGCGCCGCCCAGATGCTGTTCCTTGACACGCCCCCCCATGCCGCCGTCGCCACCTCGGTCGATGTCGC
CCGCGTGCGCCGGCTCGCCCCCTATGCCGGGCTGATCAACGCGGTTTTGCGCAAGGTCGCCGATCACGGCGCCGCCCGGC
TGGCCGGCTTTGACGATCCCTTGGCCGATCTGCCGCGCTGGCTGGGTCAAAGCTGGATCGCCGCCTATGGGGCCGAGACG
GCGGCCCGTCTGGCCGAGGCCTGCGGCCGCGAGGCGCCCCTTGATCTGACGCCGCGCGACGGCGATGGCGGGGCGTTGGC
CGAGCTGGTCGGCGGGCTGGCCCTGCCCGGCGGTTCGGTCCGCCTGCCCACCGCCACCGCCGTGCGCGAGTTGGCGGGCT
TCACCGAGGGCCAATGGTGGGTGCAAGACGCCGCCGCCGCCCTGCCGGTGCTGCTGGCCGGGGTGCCGCTCAAGGGCGCC
CGCGTCGTTGATTTCTGCGCCGCCCCCGGTGGCAAGACCCTTCAACTGGCCGCCGCCGGCGCCCAAGTCTGGGCGGTCGA
CCGCTCGGCGACCCGCCTCGCCCGCCTGCGCGAGAACCTGGAACGCACCGGCCTGGGCGACGCGGTGAACGTGGTCGAGG
GCGACGCCCTGACCTTCACCCTTACGGACGGCCAGCAAGCCGATGTGGTGTTGGTCGATGCGCCCTGCACGGCCACCGGC
ACCTTCCGCCGCCATCCCGACGCGCCCTGGCTCAAGCGGCCGGGCGACGCCGCGGCGCTGGCCGCCCTTCAGGACTCGCT
GCTTGACGCCGCCGTGCGGCTGGTACGTCCGGGCGGCGTGATCGTTTACTGCGTCTGCTCGCTTCAACCCGAGGAGGCCG
CCCCGCGCATCGAAGCCCTGCTCGCCCGCAGCCCCGAGATCAGCCGCAAGCCGATCACCGCCGGCGAAGGCGGGGTGAGC
GACGAGATGATCACCGCCGAGGGCGATGTCCGGCTGTTTCCCTTTCACCTCGACGCCGTGGGCGGCCTTGATGGCTTCTT
CATCGCCCGGCTGGTTCGCGGGGACGCGCCGCGGTGA

Upstream 100 bases:

>100_bases
CAAGACCCGCGCCCCCCGGGGCTGACCCCGGCCCTTTCCCCCCGTCCTTTCCCGCCCATTTCCATTGCCTACCCCCGAAG
GGCGGCGTACAAACCGCCCC

Downstream 100 bases:

>100_bases
GCCGCCGCCGTCGCGGGCCCGGGTTTACCGGCGCGCTGGCGCGTCCGGCTTTGGCCGTCGACAGGGGGCTGCTTGGCCAT
CCCTGGCGGCTGCTGGTCTT

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKDTATATRAAALDLLSAVLDHGRPLDDVFDAATRKLEPRDRAFARLLAATTLRRMGRLEKVIKTFLDRPLPRRAVGAKH
ILRLGAAQMLFLDTPPHAAVATSVDVARVRRLAPYAGLINAVLRKVADHGAARLAGFDDPLADLPRWLGQSWIAAYGAET
AARLAEACGREAPLDLTPRDGDGGALAELVGGLALPGGSVRLPTATAVRELAGFTEGQWWVQDAAAALPVLLAGVPLKGA
RVVDFCAAPGGKTLQLAAAGAQVWAVDRSATRLARLRENLERTGLGDAVNVVEGDALTFTLTDGQQADVVLVDAPCTATG
TFRRHPDAPWLKRPGDAAALAALQDSLLDAAVRLVRPGGVIVYCVCSLQPEEAAPRIEALLARSPEISRKPITAGEGGVS
DEMITAEGDVRLFPFHLDAVGGLDGFFIARLVRGDAPR

Sequences:

>Translated_438_residues
MKDTATATRAAALDLLSAVLDHGRPLDDVFDAATRKLEPRDRAFARLLAATTLRRMGRLEKVIKTFLDRPLPRRAVGAKH
ILRLGAAQMLFLDTPPHAAVATSVDVARVRRLAPYAGLINAVLRKVADHGAARLAGFDDPLADLPRWLGQSWIAAYGAET
AARLAEACGREAPLDLTPRDGDGGALAELVGGLALPGGSVRLPTATAVRELAGFTEGQWWVQDAAAALPVLLAGVPLKGA
RVVDFCAAPGGKTLQLAAAGAQVWAVDRSATRLARLRENLERTGLGDAVNVVEGDALTFTLTDGQQADVVLVDAPCTATG
TFRRHPDAPWLKRPGDAAALAALQDSLLDAAVRLVRPGGVIVYCVCSLQPEEAAPRIEALLARSPEISRKPITAGEGGVS
DEMITAEGDVRLFPFHLDAVGGLDGFFIARLVRGDAPR
>Mature_438_residues
MKDTATATRAAALDLLSAVLDHGRPLDDVFDAATRKLEPRDRAFARLLAATTLRRMGRLEKVIKTFLDRPLPRRAVGAKH
ILRLGAAQMLFLDTPPHAAVATSVDVARVRRLAPYAGLINAVLRKVADHGAARLAGFDDPLADLPRWLGQSWIAAYGAET
AARLAEACGREAPLDLTPRDGDGGALAELVGGLALPGGSVRLPTATAVRELAGFTEGQWWVQDAAAALPVLLAGVPLKGA
RVVDFCAAPGGKTLQLAAAGAQVWAVDRSATRLARLRENLERTGLGDAVNVVEGDALTFTLTDGQQADVVLVDAPCTATG
TFRRHPDAPWLKRPGDAAALAALQDSLLDAAVRLVRPGGVIVYCVCSLQPEEAAPRIEALLARSPEISRKPITAGEGGVS
DEMITAEGDVRLFPFHLDAVGGLDGFFIARLVRGDAPR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=233, Percent_Identity=30.9012875536481, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI76150623, Length=233, Percent_Identity=30.9012875536481, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI40316918, Length=160, Percent_Identity=37.5, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI32698918, Length=179, Percent_Identity=29.0502793296089, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11545785, Length=177, Percent_Identity=32.2033898305085, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270288818, Length=192, Percent_Identity=32.2916666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI8922322, Length=192, Percent_Identity=32.2916666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI270288816, Length=192, Percent_Identity=32.2916666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI23199998, Length=192, Percent_Identity=32.2916666666667, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=439, Percent_Identity=33.0296127562642, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081985, Length=242, Percent_Identity=32.2314049586777, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=234, Percent_Identity=28.6324786324786, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71998419, Length=172, Percent_Identity=33.7209302325581, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=172, Percent_Identity=33.7209302325581, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=230, Percent_Identity=30, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI22024126, Length=226, Percent_Identity=30.0884955752212, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920494, Length=161, Percent_Identity=32.9192546583851, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46187; Mature: 46187

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTATATRAAALDLLSAVLDHGRPLDDVFDAATRKLEPRDRAFARLLAATTLRRMGRLE
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KVIKTFLDRPLPRRAVGAKHILRLGAAQMLFLDTPPHAAVATSVDVARVRRLAPYAGLIN
HHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AVLRKVADHGAARLAGFDDPLADLPRWLGQSWIAAYGAETAARLAEACGREAPLDLTPRD
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
GDGGALAELVGGLALPGGSVRLPTATAVRELAGFTEGQWWVQDAAAALPVLLAGVPLKGA
CCCHHHHHHHCCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
RVVDFCAAPGGKTLQLAAAGAQVWAVDRSATRLARLRENLERTGLGDAVNVVEGDALTFT
CEEHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHEECCCEEEEE
LTDGQQADVVLVDAPCTATGTFRRHPDAPWLKRPGDAAALAALQDSLLDAAVRLVRPGGV
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
IVYCVCSLQPEEAAPRIEALLARSPEISRKPITAGEGGVSDEMITAEGDVRLFPFHLDAV
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEHHH
GGLDGFFIARLVRGDAPR
CCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKDTATATRAAALDLLSAVLDHGRPLDDVFDAATRKLEPRDRAFARLLAATTLRRMGRLE
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KVIKTFLDRPLPRRAVGAKHILRLGAAQMLFLDTPPHAAVATSVDVARVRRLAPYAGLIN
HHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AVLRKVADHGAARLAGFDDPLADLPRWLGQSWIAAYGAETAARLAEACGREAPLDLTPRD
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
GDGGALAELVGGLALPGGSVRLPTATAVRELAGFTEGQWWVQDAAAALPVLLAGVPLKGA
CCCHHHHHHHCCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
RVVDFCAAPGGKTLQLAAAGAQVWAVDRSATRLARLRENLERTGLGDAVNVVEGDALTFT
CEEHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHEECCCEEEEE
LTDGQQADVVLVDAPCTATGTFRRHPDAPWLKRPGDAAALAALQDSLLDAAVRLVRPGGV
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
IVYCVCSLQPEEAAPRIEALLARSPEISRKPITAGEGGVSDEMITAEGDVRLFPFHLDAV
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEHHH
GGLDGFFIARLVRGDAPR
CCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA